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The map label for this gene is recF [H]

Identifier: 187918304

GI number: 187918304

Start: 463093

End: 464160

Strand: Direct

Name: recF [H]

Synonym: BH0438A

Alternate gene names: 187918304

Gene position: 463093-464160 (Clockwise)

Preceding gene: 187918303

Following gene: 187918305

Centisome position: 50.21

GC content: 28.56

Gene sequence:

>1068_bases
ATGATAAAGAAAATTGAGTTTTTTAATTTTAAGAATATAAAAAACCAGGTTATTAATTTTGATTTTAATAATATTTATTT
TTATGGAGAAAATGGCTCAGGCAAGACTAATATTCTTGATGCTATTTATTGTCTTGCTTTTGCATCTTCATTTCTAGTTA
ATACCGATAGAGAACTTATTACATATGGCAAGACTGAGTTTTATTTAAAGTGTTTTTATAAAACTAGAGAAAAGGACGCA
GAGATTAGTTTATCATTTAGGAATGGGAAGAAAGAAATAAAGGTTAATAATAGCCTTATAAAGGATAGGAAAGATTTGAT
ATTAAATATTCCGGCTATTGTTTTTTCAAATTATGATACTGACTTTATCATTGGAGAGCCTGCTAAGAAGAGATGGTTTT
TTGATCAGGCAATAAGTCTTGTTTCTTTAAGTTATTTAGATTCTCTTAGGAAATATAGAAGGATTTTGAAGCAAAGAAAT
TTGATCTTAAAACAAGGTAATAGAGATTTGCTTAAAGTTTATAATGAAGCATTTGTTGATTATGCTCTTGAGATAATAAG
AATGAGGGAAAACTTTATCAAGCATTTTTATGAATTTTTTAAATACTATTATTCATTGATTTTTGATGTTTCTTATAATT
TAGAGATTAAATATTTGCCTTCTGTTGCATATTGTGGGAGGGATGAATTTTTGCATCTTTTGTTTTTAAAAGAAAAAGAT
GAATTTTTAAATGAGAGCACTTTAATAGGTCCGCACAGAGATTTATATGAGATATTAAGTGGTGAGAGAGTGTTTACCCA
TCATTCTTCAACAGGAGAGACCAGGGCACTTGCTTTGATCTATAGGCTTGTTCAGGTCATTATTTTCAATAAGCGATTTG
GTATAGCTCCCATTTTGCTTTTTGACGATGTATTTTTGGAATTGGATAGTACGAGACGGAAGAGAGTTTTTGATATTCTG
CCAAAGGATTCTCAATGTTTCTTTACGTTTGTAGATGAATGTTATGATATTAAACAAAAGAGTAATTTTATAGTATATAA
GGTTAAGAATGGAAAGATTGAGCTTTAA

Upstream 100 bases:

>100_bases
AGTGGAGGAGGTATATTAAAATTGAATGAACCTGATAATTTTGACTTCATTCATTTAATAATGCCCATGATACTAAACTA
AAATTCTTTCTATTGGCAAG

Downstream 100 bases:

>100_bases
AAAAGCCGCTGATGTACTTAAAGAATATTTAGATTCAGATATATGTGCTAATGAAAATCTTAATGCTAGTTTTATACTGT
CTCAAAGTTGGCAAATTATA

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MIKKIEFFNFKNIKNQVINFDFNNIYFYGENGSGKTNILDAIYCLAFASSFLVNTDRELITYGKTEFYLKCFYKTREKDA
EISLSFRNGKKEIKVNNSLIKDRKDLILNIPAIVFSNYDTDFIIGEPAKKRWFFDQAISLVSLSYLDSLRKYRRILKQRN
LILKQGNRDLLKVYNEAFVDYALEIIRMRENFIKHFYEFFKYYYSLIFDVSYNLEIKYLPSVAYCGRDEFLHLLFLKEKD
EFLNESTLIGPHRDLYEILSGERVFTHHSSTGETRALALIYRLVQVIIFNKRFGIAPILLFDDVFLELDSTRRKRVFDIL
PKDSQCFFTFVDECYDIKQKSNFIVYKVKNGKIEL

Sequences:

>Translated_355_residues
MIKKIEFFNFKNIKNQVINFDFNNIYFYGENGSGKTNILDAIYCLAFASSFLVNTDRELITYGKTEFYLKCFYKTREKDA
EISLSFRNGKKEIKVNNSLIKDRKDLILNIPAIVFSNYDTDFIIGEPAKKRWFFDQAISLVSLSYLDSLRKYRRILKQRN
LILKQGNRDLLKVYNEAFVDYALEIIRMRENFIKHFYEFFKYYYSLIFDVSYNLEIKYLPSVAYCGRDEFLHLLFLKEKD
EFLNESTLIGPHRDLYEILSGERVFTHHSSTGETRALALIYRLVQVIIFNKRFGIAPILLFDDVFLELDSTRRKRVFDIL
PKDSQCFFTFVDECYDIKQKSNFIVYKVKNGKIEL
>Mature_355_residues
MIKKIEFFNFKNIKNQVINFDFNNIYFYGENGSGKTNILDAIYCLAFASSFLVNTDRELITYGKTEFYLKCFYKTREKDA
EISLSFRNGKKEIKVNNSLIKDRKDLILNIPAIVFSNYDTDFIIGEPAKKRWFFDQAISLVSLSYLDSLRKYRRILKQRN
LILKQGNRDLLKVYNEAFVDYALEIIRMRENFIKHFYEFFKYYYSLIFDVSYNLEIKYLPSVAYCGRDEFLHLLFLKEKD
EFLNESTLIGPHRDLYEILSGERVFTHHSSTGETRALALIYRLVQVIIFNKRFGIAPILLFDDVFLELDSTRRKRVFDIL
PKDSQCFFTFVDECYDIKQKSNFIVYKVKNGKIEL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=360, Percent_Identity=23.8888888888889, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 42259; Mature: 42259

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIEFFNFKNIKNQVINFDFNNIYFYGENGSGKTNILDAIYCLAFASSFLVNTDRELI
CCCEEEECCCCCCHHHEEEEEECEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHE
TYGKTEFYLKCFYKTREKDAEISLSFRNGKKEIKVNNSLIKDRKDLILNIPAIVFSNYDT
EECCHHEEEEEEEHHCCCCCEEEEEECCCCEEEEECHHHHHCCHHHEEECCHHEECCCCC
DFIIGEPAKKRWFFDQAISLVSLSYLDSLRKYRRILKQRNLILKQGNRDLLKVYNEAFVD
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
YALEIIRMRENFIKHFYEFFKYYYSLIFDVSYNLEIKYLPSVAYCGRDEFLHLLFLKEKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECEEEEECCCEECCCCHHHEEEEEEECHH
EFLNESTLIGPHRDLYEILSGERVFTHHSSTGETRALALIYRLVQVIIFNKRFGIAPILL
HHCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
FDDVFLELDSTRRKRVFDILPKDSQCFFTFVDECYDIKQKSNFIVYKVKNGKIEL
HHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEECC
>Mature Secondary Structure
MIKKIEFFNFKNIKNQVINFDFNNIYFYGENGSGKTNILDAIYCLAFASSFLVNTDRELI
CCCEEEECCCCCCHHHEEEEEECEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHE
TYGKTEFYLKCFYKTREKDAEISLSFRNGKKEIKVNNSLIKDRKDLILNIPAIVFSNYDT
EECCHHEEEEEEEHHCCCCCEEEEEECCCCEEEEECHHHHHCCHHHEEECCHHEECCCCC
DFIIGEPAKKRWFFDQAISLVSLSYLDSLRKYRRILKQRNLILKQGNRDLLKVYNEAFVD
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
YALEIIRMRENFIKHFYEFFKYYYSLIFDVSYNLEIKYLPSVAYCGRDEFLHLLFLKEKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECEEEEECCCEECCCCHHHEEEEEEECHH
EFLNESTLIGPHRDLYEILSGERVFTHHSSTGETRALALIYRLVQVIIFNKRFGIAPILL
HHCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
FDDVFLELDSTRRKRVFDILPKDSQCFFTFVDECYDIKQKSNFIVYKVKNGKIEL
HHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]