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The map label for this gene is purA [H]

Identifier: 187918288

GI number: 187918288

Start: 443226

End: 444509

Strand: Direct

Name: purA [H]

Synonym: BH0421B

Alternate gene names: 187918288

Gene position: 443226-444509 (Clockwise)

Preceding gene: 187918287

Following gene: 187918289

Centisome position: 48.06

GC content: 31.31

Gene sequence:

>1284_bases
ATGTCAATTTACGCAGTTATTGGAACTCAATGGGGTGATGAAGGTAAGGGAAAAATTATAGATTTTCTCTCATCAAAGGC
GGATTATGTTGTAAGATTCAACGGAGGAAATAACGCTGGACATACAATCGTCGTCAATAATAAAAAATTCATTTTTAATC
TACTACCATCAGGTGTTTTACAAGGAGCAAAATGCATACTTGGCCCTGGCATAGTAATTGATCCATTAATTTTAATTAAA
GAACTTGAAACGCTAAAGCACAATAATATAAAGACAGAAATATTCATAAGCGATAAAGCGCATATAATAATGCCTTATCA
CATTAAACTCGACGAGCTTAGTGAACAAAAAAAGGGTACTTACAAAATCGGAACTACAAAACGAGGAATTGGACCTTGTT
ATGCTGACAAGATCAACAGAACAGGCATAAGAGCTACTGATTTACTTAACATGGAAACTTTTGAAAGAAAACTAAAAATA
AATTTAGATGAAAAAAATGAAATTATAGAAAAAATATATAACGATAAACCTTTCAATTACGATGATATTTTAAGTAAATA
CAAAGAATATATAGCAATACTCCAATCTGCTATCACCAATACAGAAGCAATATTAAATCATGCCATAAATTCAGGAAAAA
CTATCTTAATAGAAGGTGCTCAAGGCACAATGCTTGATATTGAACATGGAACATTTCCATTTGTAACATCAAGCAATACA
TTAATTACAGCAGCAACAGGATGTGGTATTCCTATCTCAAAAATCAAGCAAAAAATTGGCATAATAAAAGCATTCTCATC
AAGAGTTGGTTCAGGACCTTTTGTAACTGAAATCCTAGAACCTATTGGAGATAAGATTAGAGAAAAGGGGCAGGAATATG
GCTCAACAACAAAAAGACCTAGAAGAATTGGTTGGCTTGATCTCTTAACAATAAAAAAATCGATAAATCTTAACGAACTA
AACCATTTAGTCTTAACTAAACTAGACATACTAAATGACATTGAAGAACTTAAGATTTGCACAGCTTATGAATTTCAAGG
AAAAATATACGACTATATACCTGCTTCTTGTGAAATACTTGAAAAAGCTAAACCTATATACAAAGTCTTTAAGGGATTTA
AACAAGATATTAGAAATATTAGCTATTATGACGATTTGCCTATTGAGGCTAGAGAATACATTGAATTTATAGAAAGAGAA
GTGGGGGTTCAAATTTCAATGCTATCTCTTGGAGCAGAGAGAGAAAAAACTATTTTTAGGAATAAAGAATGGATCAATAT
ATAA

Upstream 100 bases:

>100_bases
TGAAATTGAAAATGATTTCATAGTCGGATATGGTATTGATTTTAATGAAAAACACAGAACTTTAAAGGATGTAGCAAAAA
TAAACAAATAGGAGATATGA

Downstream 100 bases:

>100_bases
ACCCTTTAAAATCAAGATATGCCAGTAAAGCAATGCTTTACATTTTTTCACCAAAATTTAAGTACACCACATGGAGAAAG
CTATGGTACAACTTAGCTTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSIYAVIGTQWGDEGKGKIIDFLSSKADYVVRFNGGNNAGHTIVVNNKKFIFNLLPSGVLQGAKCILGPGIVIDPLILIK
ELETLKHNNIKTEIFISDKAHIIMPYHIKLDELSEQKKGTYKIGTTKRGIGPCYADKINRTGIRATDLLNMETFERKLKI
NLDEKNEIIEKIYNDKPFNYDDILSKYKEYIAILQSAITNTEAILNHAINSGKTILIEGAQGTMLDIEHGTFPFVTSSNT
LITAATGCGIPISKIKQKIGIIKAFSSRVGSGPFVTEILEPIGDKIREKGQEYGSTTKRPRRIGWLDLLTIKKSINLNEL
NHLVLTKLDILNDIEELKICTAYEFQGKIYDYIPASCEILEKAKPIYKVFKGFKQDIRNISYYDDLPIEAREYIEFIERE
VGVQISMLSLGAEREKTIFRNKEWINI

Sequences:

>Translated_427_residues
MSIYAVIGTQWGDEGKGKIIDFLSSKADYVVRFNGGNNAGHTIVVNNKKFIFNLLPSGVLQGAKCILGPGIVIDPLILIK
ELETLKHNNIKTEIFISDKAHIIMPYHIKLDELSEQKKGTYKIGTTKRGIGPCYADKINRTGIRATDLLNMETFERKLKI
NLDEKNEIIEKIYNDKPFNYDDILSKYKEYIAILQSAITNTEAILNHAINSGKTILIEGAQGTMLDIEHGTFPFVTSSNT
LITAATGCGIPISKIKQKIGIIKAFSSRVGSGPFVTEILEPIGDKIREKGQEYGSTTKRPRRIGWLDLLTIKKSINLNEL
NHLVLTKLDILNDIEELKICTAYEFQGKIYDYIPASCEILEKAKPIYKVFKGFKQDIRNISYYDDLPIEAREYIEFIERE
VGVQISMLSLGAEREKTIFRNKEWINI
>Mature_426_residues
SIYAVIGTQWGDEGKGKIIDFLSSKADYVVRFNGGNNAGHTIVVNNKKFIFNLLPSGVLQGAKCILGPGIVIDPLILIKE
LETLKHNNIKTEIFISDKAHIIMPYHIKLDELSEQKKGTYKIGTTKRGIGPCYADKINRTGIRATDLLNMETFERKLKIN
LDEKNEIIEKIYNDKPFNYDDILSKYKEYIAILQSAITNTEAILNHAINSGKTILIEGAQGTMLDIEHGTFPFVTSSNTL
ITAATGCGIPISKIKQKIGIIKAFSSRVGSGPFVTEILEPIGDKIREKGQEYGSTTKRPRRIGWLDLLTIKKSINLNELN
HLVLTKLDILNDIEELKICTAYEFQGKIYDYIPASCEILEKAKPIYKVFKGFKQDIRNISYYDDLPIEAREYIEFIEREV
GVQISMLSLGAEREKTIFRNKEWINI

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=38.9671361502347, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=38.0841121495327, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=37.8172588832487, Blast_Score=260, Evalue=1e-69,
Organism=Escherichia coli, GI1790620, Length=418, Percent_Identity=40.4306220095694, Blast_Score=343, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI25146555, Length=436, Percent_Identity=37.8440366972477, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI25146553, Length=436, Percent_Identity=37.8440366972477, Blast_Score=296, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=40.8775981524249, Blast_Score=318, Evalue=1e-87,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=40.1891252955083, Blast_Score=306, Evalue=3e-83,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 48216; Mature: 48084

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIYAVIGTQWGDEGKGKIIDFLSSKADYVVRFNGGNNAGHTIVVNNKKFIFNLLPSGVL
CEEEEEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEECCCEEEEECCCHHHH
QGAKCILGPGIVIDPLILIKELETLKHNNIKTEIFISDKAHIIMPYHIKLDELSEQKKGT
CCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEEHHHHHHHCCCC
YKIGTTKRGIGPCYADKINRTGIRATDLLNMETFERKLKINLDEKNEIIEKIYNDKPFNY
EEECCCCCCCCCHHHHHCCCCCCCHHHCCCHHHHHHEEEECCCHHHHHHHHHHCCCCCCH
DDILSKYKEYIAILQSAITNTEAILNHAINSGKTILIEGAQGTMLDIEHGTFPFVTSSNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCEEECCCE
LITAATGCGIPISKIKQKIGIIKAFSSRVGSGPFVTEILEPIGDKIREKGQEYGSTTKRP
EEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
RRIGWLDLLTIKKSINLNELNHLVLTKLDILNDIEELKICTAYEFQGKIYDYIPASCEIL
CCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHCCHHHHHEEEEEEECCCEEECCCCHHHHH
EKAKPIYKVFKGFKQDIRNISYYDDLPIEAREYIEFIEREVGVQISMLSLGAEREKTIFR
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHC
NKEWINI
CCCCCCC
>Mature Secondary Structure 
SIYAVIGTQWGDEGKGKIIDFLSSKADYVVRFNGGNNAGHTIVVNNKKFIFNLLPSGVL
EEEEEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEECCCEEEEECCCHHHH
QGAKCILGPGIVIDPLILIKELETLKHNNIKTEIFISDKAHIIMPYHIKLDELSEQKKGT
CCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEEHHHHHHHCCCC
YKIGTTKRGIGPCYADKINRTGIRATDLLNMETFERKLKINLDEKNEIIEKIYNDKPFNY
EEECCCCCCCCCHHHHHCCCCCCCHHHCCCHHHHHHEEEECCCHHHHHHHHHHCCCCCCH
DDILSKYKEYIAILQSAITNTEAILNHAINSGKTILIEGAQGTMLDIEHGTFPFVTSSNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCEEECCCE
LITAATGCGIPISKIKQKIGIIKAFSSRVGSGPFVTEILEPIGDKIREKGQEYGSTTKRP
EEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
RRIGWLDLLTIKKSINLNELNHLVLTKLDILNDIEELKICTAYEFQGKIYDYIPASCEIL
CCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHCCHHHHHEEEEEEECCCEEECCCCHHHHH
EKAKPIYKVFKGFKQDIRNISYYDDLPIEAREYIEFIEREVGVQISMLSLGAEREKTIFR
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHC
NKEWINI
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA