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The map label for this gene is purB [H]

Identifier: 187918289

GI number: 187918289

Start: 444494

End: 445888

Strand: Direct

Name: purB [H]

Synonym: BH0421C

Alternate gene names: 187918289

Gene position: 444494-445888 (Clockwise)

Preceding gene: 187918288

Following gene: 187918294

Centisome position: 48.19

GC content: 30.47

Gene sequence:

>1395_bases
ATGGATCAATATATAAACCCTTTAAAATCAAGATATGCCAGTAAAGCAATGCTTTACATTTTTTCACCAAAATTTAAGTA
CACCACATGGAGAAAGCTATGGTACAACTTAGCTTTAGTGCAAAAAGAATTGGGAATAGATATTAGCAATAATCAACTCA
ATAATTTATCCAAACACATTGAAGATATTGATTTTGAACTTGTAGAAAAATATGAGGCAAAATTTAAACATGAAGTCATG
GCACATCTTTATGCTTATGCCGACTTAGCTGGTGATGATGCTAGAAAAATTCTACACCTTGGTGTTACAAGTGCATATTT
AATGGATAACACAGACTTAATCCAAATCAAAGAAGCTCTATTACTTATTGAAAGTAAACTGATAAAACTTATTAAAACTT
TAAGAGAATTTTCAATAAAACATAAAGATTTGGCAACACTTGCATATACACATCTACAAGAAGCACAATTAACAACTCTT
GGAAAAAGAAGTAGCTTATGGCTTCAAAGTTTGATTTTTGACTTTCAAGAACTTAAGTTCATTATGTCTAACATGTGCTT
TAGAGGAGTAAAGGGAACTATTGGAAATCAAAATAGCTTTAAAGAACTGTTTGCATCTAACTTTGAAAAGGCAAAAGCCT
TAGACATCAATCTTGCAAAAAAAATGGGGTTTGACAAAGTTTATAAAATGACTAGTCAAACTTATGATCGCAAATTTGAT
TCATCAATATTAAATTTTTTAAGCAATTTAGCTCAAAGTGCACATAAGATTACTAATGATATCAGGTTCATGCAACATCT
TAAAGAACTTGAAGAACATTTTGCAGAGCACCAAATAGGTTCATCTGCAATGCCTTACAAAAAAAACCCTATTTACAGTG
AAAGAGTAGCTTCCCTTGCTAAGTTTACAATGAGCCTACAATCAAGTGGCGGATTTATAGCTGCAACTCAATGGCTTGAG
AGAACTCTAGATGATTCAGCTTGCAAAAGACTAAACATTCCTCAAGCATTCTTAGCTACTGATGCTGCCCTGATATTACT
AGATAAAATATTCAACAATATCAGGGTAAACAAAAAAATAATTGAAAACCATGTTAAAACAGAAATGCCATTTATATTAA
CAGAAGACATATTAATGAAAGCAACAAAAAATGGTGGGGATAGACAAATATTACATGAAAAGATAAGAATTTATTCAATG
CAAGTAAGGGAAAATCTTTATTCAGGGGCAACTGAGAACGGCTTAATTAAACTAATCCTTAACGATAAAAGTTTTAGATT
AACATCTAAAGACATAGATGAAATCTTAAATCCAAATGAAAATATAGGTTTTGCCTCATACCAAGTTGAAGATTTCATTA
AAGAAATAATTGATCCTATTCTTAAAAATAAATAA

Upstream 100 bases:

>100_bases
CTAGAGAATACATTGAATTTATAGAAAGAGAAGTGGGGGTTCAAATTTCAATGCTATCTCTTGGAGCAGAGAGAGAAAAA
ACTATTTTTAGGAATAAAGA

Downstream 100 bases:

>100_bases
GGAATACTAATATTTAGAAACAAATCTATTACCCCTTATGTAAAATCGCCAAAGCTTATTCGCATACTCCTCACCTGCAT
AATCAACATTTATTCTCTTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MDQYINPLKSRYASKAMLYIFSPKFKYTTWRKLWYNLALVQKELGIDISNNQLNNLSKHIEDIDFELVEKYEAKFKHEVM
AHLYAYADLAGDDARKILHLGVTSAYLMDNTDLIQIKEALLLIESKLIKLIKTLREFSIKHKDLATLAYTHLQEAQLTTL
GKRSSLWLQSLIFDFQELKFIMSNMCFRGVKGTIGNQNSFKELFASNFEKAKALDINLAKKMGFDKVYKMTSQTYDRKFD
SSILNFLSNLAQSAHKITNDIRFMQHLKELEEHFAEHQIGSSAMPYKKNPIYSERVASLAKFTMSLQSSGGFIAATQWLE
RTLDDSACKRLNIPQAFLATDAALILLDKIFNNIRVNKKIIENHVKTEMPFILTEDILMKATKNGGDRQILHEKIRIYSM
QVRENLYSGATENGLIKLILNDKSFRLTSKDIDEILNPNENIGFASYQVEDFIKEIIDPILKNK

Sequences:

>Translated_464_residues
MDQYINPLKSRYASKAMLYIFSPKFKYTTWRKLWYNLALVQKELGIDISNNQLNNLSKHIEDIDFELVEKYEAKFKHEVM
AHLYAYADLAGDDARKILHLGVTSAYLMDNTDLIQIKEALLLIESKLIKLIKTLREFSIKHKDLATLAYTHLQEAQLTTL
GKRSSLWLQSLIFDFQELKFIMSNMCFRGVKGTIGNQNSFKELFASNFEKAKALDINLAKKMGFDKVYKMTSQTYDRKFD
SSILNFLSNLAQSAHKITNDIRFMQHLKELEEHFAEHQIGSSAMPYKKNPIYSERVASLAKFTMSLQSSGGFIAATQWLE
RTLDDSACKRLNIPQAFLATDAALILLDKIFNNIRVNKKIIENHVKTEMPFILTEDILMKATKNGGDRQILHEKIRIYSM
QVRENLYSGATENGLIKLILNDKSFRLTSKDIDEILNPNENIGFASYQVEDFIKEIIDPILKNK
>Mature_464_residues
MDQYINPLKSRYASKAMLYIFSPKFKYTTWRKLWYNLALVQKELGIDISNNQLNNLSKHIEDIDFELVEKYEAKFKHEVM
AHLYAYADLAGDDARKILHLGVTSAYLMDNTDLIQIKEALLLIESKLIKLIKTLREFSIKHKDLATLAYTHLQEAQLTTL
GKRSSLWLQSLIFDFQELKFIMSNMCFRGVKGTIGNQNSFKELFASNFEKAKALDINLAKKMGFDKVYKMTSQTYDRKFD
SSILNFLSNLAQSAHKITNDIRFMQHLKELEEHFAEHQIGSSAMPYKKNPIYSERVASLAKFTMSLQSSGGFIAATQWLE
RTLDDSACKRLNIPQAFLATDAALILLDKIFNNIRVNKKIIENHVKTEMPFILTEDILMKATKNGGDRQILHEKIRIYSM
QVRENLYSGATENGLIKLILNDKSFRLTSKDIDEILNPNENIGFASYQVEDFIKEIIDPILKNK

Specific function: Unknown

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=461, Percent_Identity=43.6008676789588, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI183227688, Length=389, Percent_Identity=44.987146529563, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17508577, Length=463, Percent_Identity=36.7170626349892, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI32564234, Length=362, Percent_Identity=39.5027624309392, Blast_Score=253, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6323391, Length=464, Percent_Identity=43.3189655172414, Blast_Score=395, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24647570, Length=458, Percent_Identity=44.9781659388646, Blast_Score=392, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 53548; Mature: 53548

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQYINPLKSRYASKAMLYIFSPKFKYTTWRKLWYNLALVQKELGIDISNNQLNNLSKHI
CCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EDIDFELVEKYEAKFKHEVMAHLYAYADLAGDDARKILHLGVTSAYLMDNTDLIQIKEAL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCCHHHHHHHH
LLIESKLIKLIKTLREFSIKHKDLATLAYTHLQEAQLTTLGKRSSLWLQSLIFDFQELKF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IMSNMCFRGVKGTIGNQNSFKELFASNFEKAKALDINLAKKMGFDKVYKMTSQTYDRKFD
HHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
SSILNFLSNLAQSAHKITNDIRFMQHLKELEEHFAEHQIGSSAMPYKKNPIYSERVASLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
KFTMSLQSSGGFIAATQWLERTLDDSACKRLNIPQAFLATDAALILLDKIFNNIRVNKKI
HHHHHHHCCCCCHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IENHVKTEMPFILTEDILMKATKNGGDRQILHEKIRIYSMQVRENLYSGATENGLIKLIL
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
NDKSFRLTSKDIDEILNPNENIGFASYQVEDFIKEIIDPILKNK
CCCCCEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDQYINPLKSRYASKAMLYIFSPKFKYTTWRKLWYNLALVQKELGIDISNNQLNNLSKHI
CCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EDIDFELVEKYEAKFKHEVMAHLYAYADLAGDDARKILHLGVTSAYLMDNTDLIQIKEAL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCCCCHHHHHHHH
LLIESKLIKLIKTLREFSIKHKDLATLAYTHLQEAQLTTLGKRSSLWLQSLIFDFQELKF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IMSNMCFRGVKGTIGNQNSFKELFASNFEKAKALDINLAKKMGFDKVYKMTSQTYDRKFD
HHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
SSILNFLSNLAQSAHKITNDIRFMQHLKELEEHFAEHQIGSSAMPYKKNPIYSERVASLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
KFTMSLQSSGGFIAATQWLERTLDDSACKRLNIPQAFLATDAALILLDKIFNNIRVNKKI
HHHHHHHCCCCCHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IENHVKTEMPFILTEDILMKATKNGGDRQILHEKIRIYSMQVRENLYSGATENGLIKLIL
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
NDKSFRLTSKDIDEILNPNENIGFASYQVEDFIKEIIDPILKNK
CCCCCEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]