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The map label for this gene is pepT [H]

Identifier: 187736424

GI number: 187736424

Start: 2357359

End: 2358612

Strand: Reverse

Name: pepT [H]

Synonym: Amuc_1942

Alternate gene names: 187736424

Gene position: 2358612-2357359 (Counterclockwise)

Preceding gene: 187736430

Following gene: 187736423

Centisome position: 88.53

GC content: 58.69

Gene sequence:

>1254_bases
ATGAACATCTCCGTTTTAGACCGTTTTTTGAAGTATGTCTCCGTGGGCTCTCAGTCGGACGCGGATTCCGCCACCGTGCC
ATCCACCCCCGGCCAGACGGAGCTGGCCCGGCTGTTGGCCGGGGAATTGAAGGACATGGGAGCGCAGGCGGAACTGGATG
ATGATTCCGGAATTGTTTACGCCTCCATCCCTTCCAACGTGGAGGGGAATGTTCCCGTCATCGGCTGGGTGGCCCATGTG
GATACGGCTCCGGGAGTCTCCGGCAGCGGAGTGAAGCCCCGCATCGTGCGCTCGTACGACGGCGGAGACATCCTGCTCAA
TCCGGAACAGGGAATGGTGCTTTCCCCCGCCGTTTTTCCCGAACTGACGGATTATTTGGGCCAGGACCTGGTCGTGACGG
ATGGAACTACCCTGCTTGGCGCGGACGACAAGGCCGGTGTGGCGGAAATCATGGATATGGCAGCCTCCTTCCTGCTGAAC
CCCGAACGGCCTCACGGCGAGATACGGATCGCCTTCACGCCGGATGAGGAAATAGGACGCGGAACGGATGCCTTTGACGT
GGCGCGATTTGGTGCGGATTTCGCCTATACCGTGGACGGAGGCGCCCTGGGGGAAATTGAATACGAAAATTTCAATGCGG
CTTCCGCAGTGGTGACGGTGCAGGGCTCTTCCATTCATCCCGGAAGCGCCAAGGGCAGGATGTTGAATGCCTGCCTGGTT
CTCATGGAATTCCAGGGAATGCTGCCCGCGTTCCAGAATCCCGCTTTTACGGAAGGGTACGAGGGCTTTTATCATTTGGA
TTCACTCCGGGGAGATGTGGAACGGGCGGCTGCGGAGTACCTCATCAGGGATCATGACAGGGAGGCCTTTGAGCGCAAGA
AGGAATTCATGCAGGAATGTGCGGCCCTGCTGAACCGTAAATACGGGGAGGGGACGGTAAAGGCGGAAATTACGGATTCC
TATTACAACATGAAGGAAAAGATACTTCCGCACATGCACCTGGTGGAACATGCCCGCAGGGCCATGGAAGCCGTGGGAGT
GAGGCCGGAAATCGTCCCTGTGCGCGGAGGCACGGACGGAGCCCACCTCTCCTTCATGGGGCTGCCGTGCCCCAACCTGT
GCGCCGGAGGCCACAACTTCCATGGAAAGTATGAATACGTCCCCGTCCGTTCCCTGGAAAAAATTTCCGCCATCCTTCAG
GAAATCGTCTCCGGTTATGCCCGGTACGGCCTGGAGCCGCCGCCAAGGGAATGA

Upstream 100 bases:

>100_bases
TACATACGGCACACCGTCCTGAATCGCAAGAATAAACCGTGTCTTGTGCTTGTGTTCGCGGGGGCGGGTGGTAGACTCTT
GCCAATCCTTGATTTTTCCC

Downstream 100 bases:

>100_bases
GGGAACATGGCGGTAAGCGGAGGTGTTGCGATTCCGTTATTCGGGAATAAGAATTTTTGCCTGTTCTTGAAATTTTTATT
GGCGGGGAGGCGCAATGCCG

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNISVLDRFLKYVSVGSQSDADSATVPSTPGQTELARLLAGELKDMGAQAELDDDSGIVYASIPSNVEGNVPVIGWVAHV
DTAPGVSGSGVKPRIVRSYDGGDILLNPEQGMVLSPAVFPELTDYLGQDLVVTDGTTLLGADDKAGVAEIMDMAASFLLN
PERPHGEIRIAFTPDEEIGRGTDAFDVARFGADFAYTVDGGALGEIEYENFNAASAVVTVQGSSIHPGSAKGRMLNACLV
LMEFQGMLPAFQNPAFTEGYEGFYHLDSLRGDVERAAAEYLIRDHDREAFERKKEFMQECAALLNRKYGEGTVKAEITDS
YYNMKEKILPHMHLVEHARRAMEAVGVRPEIVPVRGGTDGAHLSFMGLPCPNLCAGGHNFHGKYEYVPVRSLEKISAILQ
EIVSGYARYGLEPPPRE

Sequences:

>Translated_417_residues
MNISVLDRFLKYVSVGSQSDADSATVPSTPGQTELARLLAGELKDMGAQAELDDDSGIVYASIPSNVEGNVPVIGWVAHV
DTAPGVSGSGVKPRIVRSYDGGDILLNPEQGMVLSPAVFPELTDYLGQDLVVTDGTTLLGADDKAGVAEIMDMAASFLLN
PERPHGEIRIAFTPDEEIGRGTDAFDVARFGADFAYTVDGGALGEIEYENFNAASAVVTVQGSSIHPGSAKGRMLNACLV
LMEFQGMLPAFQNPAFTEGYEGFYHLDSLRGDVERAAAEYLIRDHDREAFERKKEFMQECAALLNRKYGEGTVKAEITDS
YYNMKEKILPHMHLVEHARRAMEAVGVRPEIVPVRGGTDGAHLSFMGLPCPNLCAGGHNFHGKYEYVPVRSLEKISAILQ
EIVSGYARYGLEPPPRE
>Mature_417_residues
MNISVLDRFLKYVSVGSQSDADSATVPSTPGQTELARLLAGELKDMGAQAELDDDSGIVYASIPSNVEGNVPVIGWVAHV
DTAPGVSGSGVKPRIVRSYDGGDILLNPEQGMVLSPAVFPELTDYLGQDLVVTDGTTLLGADDKAGVAEIMDMAASFLLN
PERPHGEIRIAFTPDEEIGRGTDAFDVARFGADFAYTVDGGALGEIEYENFNAASAVVTVQGSSIHPGSAKGRMLNACLV
LMEFQGMLPAFQNPAFTEGYEGFYHLDSLRGDVERAAAEYLIRDHDREAFERKKEFMQECAALLNRKYGEGTVKAEITDS
YYNMKEKILPHMHLVEHARRAMEAVGVRPEIVPVRGGTDGAHLSFMGLPCPNLCAGGHNFHGKYEYVPVRSLEKISAILQ
EIVSGYARYGLEPPPRE

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=404, Percent_Identity=50.4950495049505, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 45119; Mature: 45119

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISVLDRFLKYVSVGSQSDADSATVPSTPGQTELARLLAGELKDMGAQAELDDDSGIVY
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
ASIPSNVEGNVPVIGWVAHVDTAPGVSGSGVKPRIVRSYDGGDILLNPEQGMVLSPAVFP
EECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCEEEECCCCCCEECCHHHH
ELTDYLGQDLVVTDGTTLLGADDKAGVAEIMDMAASFLLNPERPHGEIRIAFTPDEEIGR
HHHHHCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHCC
GTDAFDVARFGADFAYTVDGGALGEIEYENFNAASAVVTVQGSSIHPGSAKGRMLNACLV
CCCHHHHHHHCCCEEEEECCCEECEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
LMEFQGMLPAFQNPAFTEGYEGFYHLDSLRGDVERAAAEYLIRDHDREAFERKKEFMQEC
HHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AALLNRKYGEGTVKAEITDSYYNMKEKILPHMHLVEHARRAMEAVGVRPEIVPVRGGTDG
HHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
AHLSFMGLPCPNLCAGGHNFHGKYEYVPVRSLEKISAILQEIVSGYARYGLEPPPRE
CEEEEECCCCCHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNISVLDRFLKYVSVGSQSDADSATVPSTPGQTELARLLAGELKDMGAQAELDDDSGIVY
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
ASIPSNVEGNVPVIGWVAHVDTAPGVSGSGVKPRIVRSYDGGDILLNPEQGMVLSPAVFP
EECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCEEEECCCCCCEECCHHHH
ELTDYLGQDLVVTDGTTLLGADDKAGVAEIMDMAASFLLNPERPHGEIRIAFTPDEEIGR
HHHHHCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHCC
GTDAFDVARFGADFAYTVDGGALGEIEYENFNAASAVVTVQGSSIHPGSAKGRMLNACLV
CCCHHHHHHHCCCEEEEECCCEECEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
LMEFQGMLPAFQNPAFTEGYEGFYHLDSLRGDVERAAAEYLIRDHDREAFERKKEFMQEC
HHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AALLNRKYGEGTVKAEITDSYYNMKEKILPHMHLVEHARRAMEAVGVRPEIVPVRGGTDG
HHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
AHLSFMGLPCPNLCAGGHNFHGKYEYVPVRSLEKISAILQEIVSGYARYGLEPPPRE
CEEEEECCCCCHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA