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The map label for this gene is purB [H]

Identifier: 187736423

GI number: 187736423

Start: 2355681

End: 2357105

Strand: Reverse

Name: purB [H]

Synonym: Amuc_1941

Alternate gene names: 187736423

Gene position: 2357105-2355681 (Counterclockwise)

Preceding gene: 187736424

Following gene: 187736422

Centisome position: 88.48

GC content: 59.86

Gene sequence:

>1425_bases
ATGAGCGTGATTCCCAATGTCCTGGCTGAAAGGTACGCCTCCGCCGCCATGAAATCCATCTGGTCCGCAGAGGGCCGCAT
CATTCTGGAACGCGAATTCTGGATTGCCGTGATGAAAGCCCAGAAGGATCTGGGGCTGGATATTCCGGACGGGGTGATTG
AGGCCTATGAACGGGTGAAAGACCAGGTGAACCTTCCCTCCATTGACGCACGCGAACGCGTGACGCGCCACGATGTGAAG
GCGCGCATAGAAGAATTTTGCGAGCTGGCCGGATGCGAGCATATCCATAAGGGAATGACCTCCCGGGACCTGACGGAAAA
TGTGGAACAGCTCCAGATTTGGAAGTCCATGCAAATCATCCGGGACAAGGCCGTGGCCGTGTTGAACCGCATGAGCGCCC
TGGCTGAACAGTGGAAGCACGTGACCATTGCCGGACGCACGCACAATGTAGCCGCGCAGACCACAACCATGGGCAAGCGT
GTCGCCATGTTCGGGGAAGACATCGTGCACGGCCTGGGGGCCTGGATTTCCCTCATGGACCGTTACAGCGTGCGTGGACT
GAAGGGCGCCGTTGGAACCCAGCTTGACCAGCTTTCCCTCTTCGGGCAGGATGCCGTCCGCGTGCTGGAACTGGAAGACC
GCGTGTGCGCCCACCTGGGCATCCCTGCCAAGTGGATGAATGTGGGGCAGGTATATCCGCGTTCTCTGGACTTTTCCGTG
GTTTCCGGGCTGGTGGAACTCACCTCCGGCTGCTCCTCCTTCGCCAAGACCCTGCGCCTGATGGCCGGCCATGAACTGGC
CACGGAGGGGTTCGCCAAGGGGCAGACTGGCTCCAGCGCCATGCCGCACAAGATGAACGCCCGCTCCTGCGAGCGCGTCA
ACGGCTTCCACGTCATTTTGAAAGGCTTCCTGATGATGATTTCCGGGCTGGCCGGGGATCAGTGGAATGAAGGGGACGTT
TCCTGCTCCGTGGTGCGCCGCGTAGTCATGCCTGATTCCTTTTACGCTGCGGACGGCCTGTTTGAAACCTTCCTGACCGT
GTTGAACCAGATGGGCGTGTATGAGGCCGTGGTGGCTGCGGAATTGCGCCGCTACCTGCCTTTCCTGATGACAACCACCA
TACTGATGGAGGCCGTCAAGCGCGGCATTGGCCGGGAGACCGCCCATGAGGTGATCAAGGAACATGCCGTGGCTGTCTCC
AATGACCTGCGCGCCGGGAAAATCATGGAAAACAATTTGCTGGACCGCCTGGCGGAAGACGGGCGCCTGGGAATAGACCG
GGCGGACCTTCAGCGCATTTTTGATTCCAATTCCTCCGCTACGGGCATGGCTGACGCCCAGGTGGAAGCATTCCGCTCCA
TGGTGAAGGAAGTGACGGACAGGTTTCCGGACGGGGCGGGCTACCAGCCCGGGGATATCCTGTAA

Upstream 100 bases:

>100_bases
CTAATCCGTGAAGCTGCGGGAAAGTGCTTGCTTTTTTCCCTTCCGTCATGATCATGGCGGCTCACCGTTTTTGATAACCC
CCGCATATACACATTTTGAT

Downstream 100 bases:

>100_bases
AAAGAACCTCGGAAAGAAGCCGGAACTCCCGGCGCACGCGTTTTTTCCAGACGCCGCCCTTTCGGACGGCGTCTGTATTT
TTTATGCCGTCCGCATACGT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSVIPNVLAERYASAAMKSIWSAEGRIILEREFWIAVMKAQKDLGLDIPDGVIEAYERVKDQVNLPSIDARERVTRHDVK
ARIEEFCELAGCEHIHKGMTSRDLTENVEQLQIWKSMQIIRDKAVAVLNRMSALAEQWKHVTIAGRTHNVAAQTTTMGKR
VAMFGEDIVHGLGAWISLMDRYSVRGLKGAVGTQLDQLSLFGQDAVRVLELEDRVCAHLGIPAKWMNVGQVYPRSLDFSV
VSGLVELTSGCSSFAKTLRLMAGHELATEGFAKGQTGSSAMPHKMNARSCERVNGFHVILKGFLMMISGLAGDQWNEGDV
SCSVVRRVVMPDSFYAADGLFETFLTVLNQMGVYEAVVAAELRRYLPFLMTTTILMEAVKRGIGRETAHEVIKEHAVAVS
NDLRAGKIMENNLLDRLAEDGRLGIDRADLQRIFDSNSSATGMADAQVEAFRSMVKEVTDRFPDGAGYQPGDIL

Sequences:

>Translated_474_residues
MSVIPNVLAERYASAAMKSIWSAEGRIILEREFWIAVMKAQKDLGLDIPDGVIEAYERVKDQVNLPSIDARERVTRHDVK
ARIEEFCELAGCEHIHKGMTSRDLTENVEQLQIWKSMQIIRDKAVAVLNRMSALAEQWKHVTIAGRTHNVAAQTTTMGKR
VAMFGEDIVHGLGAWISLMDRYSVRGLKGAVGTQLDQLSLFGQDAVRVLELEDRVCAHLGIPAKWMNVGQVYPRSLDFSV
VSGLVELTSGCSSFAKTLRLMAGHELATEGFAKGQTGSSAMPHKMNARSCERVNGFHVILKGFLMMISGLAGDQWNEGDV
SCSVVRRVVMPDSFYAADGLFETFLTVLNQMGVYEAVVAAELRRYLPFLMTTTILMEAVKRGIGRETAHEVIKEHAVAVS
NDLRAGKIMENNLLDRLAEDGRLGIDRADLQRIFDSNSSATGMADAQVEAFRSMVKEVTDRFPDGAGYQPGDIL
>Mature_473_residues
SVIPNVLAERYASAAMKSIWSAEGRIILEREFWIAVMKAQKDLGLDIPDGVIEAYERVKDQVNLPSIDARERVTRHDVKA
RIEEFCELAGCEHIHKGMTSRDLTENVEQLQIWKSMQIIRDKAVAVLNRMSALAEQWKHVTIAGRTHNVAAQTTTMGKRV
AMFGEDIVHGLGAWISLMDRYSVRGLKGAVGTQLDQLSLFGQDAVRVLELEDRVCAHLGIPAKWMNVGQVYPRSLDFSVV
SGLVELTSGCSSFAKTLRLMAGHELATEGFAKGQTGSSAMPHKMNARSCERVNGFHVILKGFLMMISGLAGDQWNEGDVS
CSVVRRVVMPDSFYAADGLFETFLTVLNQMGVYEAVVAAELRRYLPFLMTTTILMEAVKRGIGRETAHEVIKEHAVAVSN
DLRAGKIMENNLLDRLAEDGRLGIDRADLQRIFDSNSSATGMADAQVEAFRSMVKEVTDRFPDGAGYQPGDIL

Specific function: Unknown

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=449, Percent_Identity=30.2895322939866, Blast_Score=195, Evalue=1e-49,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=30.4461942257218, Blast_Score=177, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17508577, Length=396, Percent_Identity=28.5353535353535, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32564234, Length=343, Percent_Identity=29.4460641399417, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323391, Length=453, Percent_Identity=31.7880794701987, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24647570, Length=462, Percent_Identity=29.6536796536797, Blast_Score=183, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 52423; Mature: 52292

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIPNVLAERYASAAMKSIWSAEGRIILEREFWIAVMKAQKDLGLDIPDGVIEAYERVK
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
DQVNLPSIDARERVTRHDVKARIEEFCELAGCEHIHKGMTSRDLTENVEQLQIWKSMQII
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RDKAVAVLNRMSALAEQWKHVTIAGRTHNVAAQTTTMGKRVAMFGEDIVHGLGAWISLMD
HHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYSVRGLKGAVGTQLDQLSLFGQDAVRVLELEDRVCAHLGIPAKWMNVGQVYPRSLDFSV
HHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHH
VSGLVELTSGCSSFAKTLRLMAGHELATEGFAKGQTGSSAMPHKMNARSCERVNGFHVIL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KGFLMMISGLAGDQWNEGDVSCSVVRRVVMPDSFYAADGLFETFLTVLNQMGVYEAVVAA
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELRRYLPFLMTTTILMEAVKRGIGRETAHEVIKEHAVAVSNDLRAGKIMENNLLDRLAED
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
GRLGIDRADLQRIFDSNSSATGMADAQVEAFRSMVKEVTDRFPDGAGYQPGDIL
CCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SVIPNVLAERYASAAMKSIWSAEGRIILEREFWIAVMKAQKDLGLDIPDGVIEAYERVK
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
DQVNLPSIDARERVTRHDVKARIEEFCELAGCEHIHKGMTSRDLTENVEQLQIWKSMQII
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RDKAVAVLNRMSALAEQWKHVTIAGRTHNVAAQTTTMGKRVAMFGEDIVHGLGAWISLMD
HHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYSVRGLKGAVGTQLDQLSLFGQDAVRVLELEDRVCAHLGIPAKWMNVGQVYPRSLDFSV
HHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHH
VSGLVELTSGCSSFAKTLRLMAGHELATEGFAKGQTGSSAMPHKMNARSCERVNGFHVIL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KGFLMMISGLAGDQWNEGDVSCSVVRRVVMPDSFYAADGLFETFLTVLNQMGVYEAVVAA
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELRRYLPFLMTTTILMEAVKRGIGRETAHEVIKEHAVAVSNDLRAGKIMENNLLDRLAED
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
GRLGIDRADLQRIFDSNSSATGMADAQVEAFRSMVKEVTDRFPDGAGYQPGDIL
CCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]