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The map label for this gene is dus1 [H]

Identifier: 187736076

GI number: 187736076

Start: 1909268

End: 1910272

Strand: Reverse

Name: dus1 [H]

Synonym: Amuc_1587

Alternate gene names: 187736076

Gene position: 1910272-1909268 (Counterclockwise)

Preceding gene: 187736077

Following gene: 187736075

Centisome position: 71.7

GC content: 56.92

Gene sequence:

>1005_bases
ATGACTTTTTCCAACCGTCCCGCTCCCCTCCCGTTCTTCAAAACGGATTTCCCGCTGTTCCTGGCTCCAATGGCTGGAGT
GACGGACCCCATTTTCCGCACTATCTGCAAGGAGCTTGGAGTGGATGTAATGGTAACGGAATTCGTCTCTGCGGAAGGAA
TTCTCCAGGCATGGGAAAGAAACCGCAGGTACACAAAGATAGAAAATGCCCACCGCCCCATAGGCATCCAGCTCTTCGGA
GCGGATGGGAACCACATGGGAGAGGCGGCCAGAATTATCCTGGACCATGAAAAGCCGGATTTTATCGATATCAACTTCGG
CTGCCCGGTTCCCAAGGTGGTTGGCAAAAATGGAGGTTCCTCCCTCCTGAAAGACCTTCCCCTGCTGGCTTCCGTTGCCG
GAGGCGTCGTGAAAGCCGTAGGAGAGAGAATCCCCGTCACGGCCAAAATACGTATTGGCTGGGATTTTCAGAGCCTGTGC
GCCATGGACGCGTGCCGCCTGCTGGAGAATGAGGGAATTTCCATGATCACCATTCACGGGCGCACCCGCTCCCAGCAGTA
TTCCGGAACGGCCAACTGGGACATTATTGACGAATGCGCGCGGGCCATGTCCGTGCCGGTCATCGGGAACGGGGATATTT
CCACCCCGCAGGCCGTAGCTTATGTAAAAGAAAATACGGCCGTCAGCGGCGTCATGATTGGCCGTGCGGCCATGGAAAAC
CCATGGATTTTCGGAGACGCCAAGTATTATCTGCTGCACGGAACCATGCCGCCGGCCAGAGACCCGCAGGAAAAGGCGGC
CCTGATTTTGCGGCATGCCCGGATGGCCCTGGAATCCCGCCATTATGGGGACGAACTTCACACCATGCGCCATATGAGGT
CACGAATCCTGGCTTATACGAAGGGATTTCCCGGCGCCAAGGAAATGCGTTCCCGCCTGGTGCGGGTATCCAGCATGCCG
GAGCTGGAAGACCTGCTTTCCAAGCTCCCCCGCCAGAAGCCCTGA

Upstream 100 bases:

>100_bases
GTTTGCACAGCACCGGCATCTTGCACCTTCCCTTCTTCGCCAGAACAGGCAAGATGCTTGAATGGGACGGGAACCCGTGC
TAGCCTCATCCTAAGGAATC

Downstream 100 bases:

>100_bases
GTACAGGGAAAATCCCGCTTTCCGGAAGATTCCGCAGGGTTTGGAAAGCGGCGGAGAGGGAAGGAGGTTCTTCCCGCCTC
CGGACACGCCCGAAAAAAAA

Product: TIM-barrel protein, nifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTFSNRPAPLPFFKTDFPLFLAPMAGVTDPIFRTICKELGVDVMVTEFVSAEGILQAWERNRRYTKIENAHRPIGIQLFG
ADGNHMGEAARIILDHEKPDFIDINFGCPVPKVVGKNGGSSLLKDLPLLASVAGGVVKAVGERIPVTAKIRIGWDFQSLC
AMDACRLLENEGISMITIHGRTRSQQYSGTANWDIIDECARAMSVPVIGNGDISTPQAVAYVKENTAVSGVMIGRAAMEN
PWIFGDAKYYLLHGTMPPARDPQEKAALILRHARMALESRHYGDELHTMRHMRSRILAYTKGFPGAKEMRSRLVRVSSMP
ELEDLLSKLPRQKP

Sequences:

>Translated_334_residues
MTFSNRPAPLPFFKTDFPLFLAPMAGVTDPIFRTICKELGVDVMVTEFVSAEGILQAWERNRRYTKIENAHRPIGIQLFG
ADGNHMGEAARIILDHEKPDFIDINFGCPVPKVVGKNGGSSLLKDLPLLASVAGGVVKAVGERIPVTAKIRIGWDFQSLC
AMDACRLLENEGISMITIHGRTRSQQYSGTANWDIIDECARAMSVPVIGNGDISTPQAVAYVKENTAVSGVMIGRAAMEN
PWIFGDAKYYLLHGTMPPARDPQEKAALILRHARMALESRHYGDELHTMRHMRSRILAYTKGFPGAKEMRSRLVRVSSMP
ELEDLLSKLPRQKP
>Mature_333_residues
TFSNRPAPLPFFKTDFPLFLAPMAGVTDPIFRTICKELGVDVMVTEFVSAEGILQAWERNRRYTKIENAHRPIGIQLFGA
DGNHMGEAARIILDHEKPDFIDINFGCPVPKVVGKNGGSSLLKDLPLLASVAGGVVKAVGERIPVTAKIRIGWDFQSLCA
MDACRLLENEGISMITIHGRTRSQQYSGTANWDIIDECARAMSVPVIGNGDISTPQAVAYVKENTAVSGVMIGRAAMENP
WIFGDAKYYLLHGTMPPARDPQEKAALILRHARMALESRHYGDELHTMRHMRSRILAYTKGFPGAKEMRSRLVRVSSMPE
LEDLLSKLPRQKP

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=231, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI239788462, Length=231, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=29.646017699115, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=27.8260869565217, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=27.7551020408163, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789660, Length=258, Percent_Identity=38.7596899224806, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=32.3651452282158, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17543114, Length=242, Percent_Identity=32.2314049586777, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=29.6803652968037, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=237, Percent_Identity=28.6919831223629, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17510279, Length=243, Percent_Identity=26.3374485596708, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=27.7533039647577, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323433, Length=328, Percent_Identity=23.4756097560976, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=234, Percent_Identity=26.0683760683761, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24585320, Length=234, Percent_Identity=36.7521367521368, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24580595, Length=249, Percent_Identity=29.718875502008, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19920448, Length=249, Percent_Identity=29.718875502008, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19921524, Length=234, Percent_Identity=25.6410256410256, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI45549423, Length=246, Percent_Identity=26.8292682926829, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36999; Mature: 36868

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSNRPAPLPFFKTDFPLFLAPMAGVTDPIFRTICKELGVDVMVTEFVSAEGILQAWER
CCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHC
NRRYTKIENAHRPIGIQLFGADGNHMGEAARIILDHEKPDFIDINFGCPVPKVVGKNGGS
CCCEEECCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCCHHHCCCCCH
SLLKDLPLLASVAGGVVKAVGERIPVTAKIRIGWDFQSLCAMDACRLLENEGISMITIHG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEC
RTRSQQYSGTANWDIIDECARAMSVPVIGNGDISTPQAVAYVKENTAVSGVMIGRAAMEN
CCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCHHEEEEECCCCCCEEEECHHHCCC
PWIFGDAKYYLLHGTMPPARDPQEKAALILRHARMALESRHYGDELHTMRHMRSRILAYT
CEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KGFPGAKEMRSRLVRVSSMPELEDLLSKLPRQKP
CCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure 
TFSNRPAPLPFFKTDFPLFLAPMAGVTDPIFRTICKELGVDVMVTEFVSAEGILQAWER
CCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHC
NRRYTKIENAHRPIGIQLFGADGNHMGEAARIILDHEKPDFIDINFGCPVPKVVGKNGGS
CCCEEECCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCCHHHCCCCCH
SLLKDLPLLASVAGGVVKAVGERIPVTAKIRIGWDFQSLCAMDACRLLENEGISMITIHG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEC
RTRSQQYSGTANWDIIDECARAMSVPVIGNGDISTPQAVAYVKENTAVSGVMIGRAAMEN
CCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCCCCCHHEEEEECCCCCCEEEECHHHCCC
PWIFGDAKYYLLHGTMPPARDPQEKAALILRHARMALESRHYGDELHTMRHMRSRILAYT
CEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KGFPGAKEMRSRLVRVSSMPELEDLLSKLPRQKP
CCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]