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The map label for this gene is mdh

Identifier: 187735927

GI number: 187735927

Start: 1722304

End: 1723293

Strand: Direct

Name: mdh

Synonym: Amuc_1436

Alternate gene names: 187735927

Gene position: 1722304-1723293 (Clockwise)

Preceding gene: 187735925

Following gene: 187735928

Centisome position: 64.65

GC content: 58.38

Gene sequence:

>990_bases
ATGAAAACACCTATCACCGTTACCGTTACAGGAGCCGCCGGCCAAATTGCCTATTCCCTTCTGTTCCGTATTGCCTCCGG
CAGCATGCTGGGGCCGGATCAGCCGATCAACCTGCGCCTGCTGGAAATTCCCCCGGCCATGAATGCTCTGGAAGGGGTGG
TGATGGAGTTGCGTGACGCCGCGTTCCCGCTGGTTAATGAAATTGTCCCGACCAGCGATCCTGATGAAGCGTTCGCCGGC
GCCAACTGGTGCCTGCTGGTAGGCTCCGTTCCCCGCAAGGCCGGTATGGAACGCAAGGACCTGCTGGATATCAACGGCAA
GGTGTTCATCGGCCAGGGGCAGGCCATTGCCCGCAGCGCCGCCAAGGATGTGCGTGTGCTGGTGGTCGGCAACCCCTGCA
ATACGAATGCCCTTATTGCCATGCACAATGCGAGCGGCGTTCCCTCCGACCGCTTTTTTGCCATGACTCGCCTGGATGAA
AACCGTGCCAAGAGCCAGCTTGCGGAAAAGGCCGGCGTCCATGTGACGGAAGTGACCAACATGGCCATCTGGGGCAATCA
TTCCTCCACCCAGTACCCTGATTTCACCAACGCCAGGATTGGCGGAAAGCCTGTGACGGAAGTCATCAAGGATACGGAAT
GGCTTAAGGGCGATTTCATTACCACCGTGCAGCAGCGCGGTGCCGCCATTATCAAGGCACGCGGCGCTTCTTCCGCCGCT
TCCGCCGCTTCCGCAGCCGTGGATACCGTCCGCAGCCTGGCTACCCAGACTCCGGAAGGCGACTGGTATTCCGTGGCCGT
TTGCTCCGACGGTTCCTACGGCATTGAAAAAGGTCTTATCTGCTCCTTCCCGGTCCGCACCACCAAGGATGGCGGCTGGG
AAATCGTGCAGGGGCTGCCGGTTGATGCGTTCTCCCGTGAAAAAATTGACGCTACCGTTAATGAACTGAAGGAAGAACGT
GACGCTGTTTCCTCTTTGTTGAAGCATTAA

Upstream 100 bases:

>100_bases
TTTTCTGTCAAAATAAAAAGTTCCTGATTGAGCGCCTCCTTTCCTTGTGGTATAACCGTGACCAGACAGGAAAATATCTG
TTTATTTTAATTTGAACGTT

Downstream 100 bases:

>100_bases
TGTTGATTTGCTCTTGCGCCGGGTTTCCCGTTTTCGGGAAGCCCGGTTTTTTATGTCCTGAAGATTCCATAAACCTCATT
TTCTGCGGGACAGAAGTTTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKTPITVTVTGAAGQIAYSLLFRIASGSMLGPDQPINLRLLEIPPAMNALEGVVMELRDAAFPLVNEIVPTSDPDEAFAG
ANWCLLVGSVPRKAGMERKDLLDINGKVFIGQGQAIARSAAKDVRVLVVGNPCNTNALIAMHNASGVPSDRFFAMTRLDE
NRAKSQLAEKAGVHVTEVTNMAIWGNHSSTQYPDFTNARIGGKPVTEVIKDTEWLKGDFITTVQQRGAAIIKARGASSAA
SAASAAVDTVRSLATQTPEGDWYSVAVCSDGSYGIEKGLICSFPVRTTKDGGWEIVQGLPVDAFSREKIDATVNELKEER
DAVSSLLKH

Sequences:

>Translated_329_residues
MKTPITVTVTGAAGQIAYSLLFRIASGSMLGPDQPINLRLLEIPPAMNALEGVVMELRDAAFPLVNEIVPTSDPDEAFAG
ANWCLLVGSVPRKAGMERKDLLDINGKVFIGQGQAIARSAAKDVRVLVVGNPCNTNALIAMHNASGVPSDRFFAMTRLDE
NRAKSQLAEKAGVHVTEVTNMAIWGNHSSTQYPDFTNARIGGKPVTEVIKDTEWLKGDFITTVQQRGAAIIKARGASSAA
SAASAAVDTVRSLATQTPEGDWYSVAVCSDGSYGIEKGLICSFPVRTTKDGGWEIVQGLPVDAFSREKIDATVNELKEER
DAVSSLLKH
>Mature_329_residues
MKTPITVTVTGAAGQIAYSLLFRIASGSMLGPDQPINLRLLEIPPAMNALEGVVMELRDAAFPLVNEIVPTSDPDEAFAG
ANWCLLVGSVPRKAGMERKDLLDINGKVFIGQGQAIARSAAKDVRVLVVGNPCNTNALIAMHNASGVPSDRFFAMTRLDE
NRAKSQLAEKAGVHVTEVTNMAIWGNHSSTQYPDFTNARIGGKPVTEVIKDTEWLKGDFITTVQQRGAAIIKARGASSAA
SAASAAVDTVRSLATQTPEGDWYSVAVCSDGSYGIEKGLICSFPVRTTKDGGWEIVQGLPVDAFSREKIDATVNELKEER
DAVSSLLKH

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=332, Percent_Identity=53.9156626506024, Blast_Score=348, Evalue=3e-96,
Organism=Homo sapiens, GI89886456, Length=330, Percent_Identity=24.8484848484848, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1789632, Length=326, Percent_Identity=26.9938650306748, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17561064, Length=327, Percent_Identity=51.6819571865443, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI32566798, Length=269, Percent_Identity=49.0706319702602, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI32566800, Length=172, Percent_Identity=52.9069767441861, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24583394, Length=331, Percent_Identity=53.4743202416918, Blast_Score=322, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24647881, Length=321, Percent_Identity=24.2990654205607, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_AKKM8 (B2UKY5)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001878039.1
- ProteinModelPortal:   B2UKY5
- SMR:   B2UKY5
- GeneID:   6274593
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_1436
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35097; Mature: 35097

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 187-187 BINDING 92-92 BINDING 98-98 BINDING 105-105 BINDING 112-112 BINDING 131-131 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPITVTVTGAAGQIAYSLLFRIASGSMLGPDQPINLRLLEIPPAMNALEGVVMELRDA
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
AFPLVNEIVPTSDPDEAFAGANWCLLVGSVPRKAGMERKDLLDINGKVFIGQGQAIARSA
HHHHHHHHCCCCCCCHHHCCCCEEEEEECCCHHHCCCHHHEEECCCEEEEECCHHHHHHH
AKDVRVLVVGNPCNTNALIAMHNASGVPSDRFFAMTRLDENRAKSQLAEKAGVHVTEVTN
CCCEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHCCEEEEEEE
MAIWGNHSSTQYPDFTNARIGGKPVTEVIKDTEWLKGDFITTVQQRGAAIIKARGASSAA
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCHH
SAASAAVDTVRSLATQTPEGDWYSVAVCSDGSYGIEKGLICSFPVRTTKDGGWEIVQGLP
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCCCCEEEEECCC
VDAFSREKIDATVNELKEERDAVSSLLKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTPITVTVTGAAGQIAYSLLFRIASGSMLGPDQPINLRLLEIPPAMNALEGVVMELRDA
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
AFPLVNEIVPTSDPDEAFAGANWCLLVGSVPRKAGMERKDLLDINGKVFIGQGQAIARSA
HHHHHHHHCCCCCCCHHHCCCCEEEEEECCCHHHCCCHHHEEECCCEEEEECCHHHHHHH
AKDVRVLVVGNPCNTNALIAMHNASGVPSDRFFAMTRLDENRAKSQLAEKAGVHVTEVTN
CCCEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHCCEEEEEEE
MAIWGNHSSTQYPDFTNARIGGKPVTEVIKDTEWLKGDFITTVQQRGAAIIKARGASSAA
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCHH
SAASAAVDTVRSLATQTPEGDWYSVAVCSDGSYGIEKGLICSFPVRTTKDGGWEIVQGLP
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCCCCEEEEECCC
VDAFSREKIDATVNELKEERDAVSSLLKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA