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The map label for this gene is cysK [H]

Identifier: 187735794

GI number: 187735794

Start: 1577395

End: 1578324

Strand: Reverse

Name: cysK [H]

Synonym: Amuc_1301

Alternate gene names: 187735794

Gene position: 1578324-1577395 (Counterclockwise)

Preceding gene: 187735795

Following gene: 187735793

Centisome position: 59.24

GC content: 51.94

Gene sequence:

>930_bases
ATGAACATTTACACCTCCATATCGGAACTGGTCGGGAAAACACCTCTTCTGGAGCCAAGGAATTATGCTCAGGCACATCG
TCTGAATGCCCGGCTCCTGCTCAAGCTGGACCACTTGAACCCGGCAGGCAGCATGAAAGACCGTATCGCACTCGCCATGA
TCAACGAGGCAGAAGCATCGGGCCAACTCAAATCGGGATCTGTCATCATTGAACCAACCAGCGGCAACACGGGCATTGGC
CTGGCGGCTATTGCTGCTTCCCGGGGATACCGCATCATTCTTACCATGCCGGAAAGCATGAGCGTTGAACGACGAAATAT
TCTAAAAGCCTACGGAGCCGATATTGTCCTTACAGAAGACAGCCAAGGAATGAAAGGAGCCATCGCCAAAGCCCAGGATC
TGGCGGCCACAACTCCGGGCTCCTTCATTCCCAGTCAATTCACCAACCCAACCAATCCCAGAGCCCACTATGAAACAACA
GGGGTTGAAATATGGGAAGATACGGACGGAGAAGTAGATATCGTCGTAGCCGGAGTAGGCACCGGGGGAACAATAACCGG
CATAGGCAGACTGCTCAAGGAACGCAAACCCGGAGGAATCACCGTCATTGCCGTGGAACCGGCAGATTCCCCCGTATTAT
CCGCCGGCCAGGCCGGAAGCCATCAAATTCAAGGAATAGGAGCTGGCTTTATCCCAGAAACCCTGGATACGGATATCTAT
GATGAAGTCATCACAGTTTCCAACGAAGATGCCTTTTCCACAGCTAAAGAATTGGGCGTCAAAGAAGGCATTCTAACTGG
GATTTCCAGCGGAGCTGCCCTATGGGCTGCCGTCCAGGTTGCTAAAAGAAAAGAGAACACAGGGAAAAATGTAGTAGTGA
TCCTCCCCGATTCAGCGGATCGGTACTACTCAACTCCTCTGTTCATCTGA

Upstream 100 bases:

>100_bases
AATTCACATTGACACTTCCAGAATATAAGAGTAACTAATTCCTATTATTCCTATCGGAATACAAAAGAAATCTAAATCCA
TCCCTGTAAAGTACGACACG

Downstream 100 bases:

>100_bases
CATCCCGTTCTTTTCTACCGGATACGGCTATCCACCTTCAACCAGCGCCAACATTCATGAACGCTCATTCCCCAACCGCT
ACAGACACGTTGCGACAAGC

Product: cysteine synthase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNIYTSISELVGKTPLLEPRNYAQAHRLNARLLLKLDHLNPAGSMKDRIALAMINEAEASGQLKSGSVIIEPTSGNTGIG
LAAIAASRGYRIILTMPESMSVERRNILKAYGADIVLTEDSQGMKGAIAKAQDLAATTPGSFIPSQFTNPTNPRAHYETT
GVEIWEDTDGEVDIVVAGVGTGGTITGIGRLLKERKPGGITVIAVEPADSPVLSAGQAGSHQIQGIGAGFIPETLDTDIY
DEVITVSNEDAFSTAKELGVKEGILTGISSGAALWAAVQVAKRKENTGKNVVVILPDSADRYYSTPLFI

Sequences:

>Translated_309_residues
MNIYTSISELVGKTPLLEPRNYAQAHRLNARLLLKLDHLNPAGSMKDRIALAMINEAEASGQLKSGSVIIEPTSGNTGIG
LAAIAASRGYRIILTMPESMSVERRNILKAYGADIVLTEDSQGMKGAIAKAQDLAATTPGSFIPSQFTNPTNPRAHYETT
GVEIWEDTDGEVDIVVAGVGTGGTITGIGRLLKERKPGGITVIAVEPADSPVLSAGQAGSHQIQGIGAGFIPETLDTDIY
DEVITVSNEDAFSTAKELGVKEGILTGISSGAALWAAVQVAKRKENTGKNVVVILPDSADRYYSTPLFI
>Mature_309_residues
MNIYTSISELVGKTPLLEPRNYAQAHRLNARLLLKLDHLNPAGSMKDRIALAMINEAEASGQLKSGSVIIEPTSGNTGIG
LAAIAASRGYRIILTMPESMSVERRNILKAYGADIVLTEDSQGMKGAIAKAQDLAATTPGSFIPSQFTNPTNPRAHYETT
GVEIWEDTDGEVDIVVAGVGTGGTITGIGRLLKERKPGGITVIAVEPADSPVLSAGQAGSHQIQGIGAGFIPETLDTDIY
DEVITVSNEDAFSTAKELGVKEGILTGISSGAALWAAVQVAKRKENTGKNVVVILPDSADRYYSTPLFI

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=314, Percent_Identity=42.6751592356688, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI295821200, Length=314, Percent_Identity=42.6751592356688, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI4557415, Length=314, Percent_Identity=42.6751592356688, Blast_Score=234, Evalue=9e-62,
Organism=Escherichia coli, GI1788754, Length=316, Percent_Identity=51.8987341772152, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI2367138, Length=305, Percent_Identity=42.2950819672131, Blast_Score=230, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI17535051, Length=301, Percent_Identity=49.8338870431894, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17562970, Length=299, Percent_Identity=47.4916387959866, Blast_Score=283, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI115535073, Length=300, Percent_Identity=52.6666666666667, Blast_Score=281, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=47.7815699658703, Blast_Score=277, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17561720, Length=299, Percent_Identity=47.8260869565217, Blast_Score=255, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=47.4178403755869, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17534315, Length=315, Percent_Identity=41.2698412698413, Blast_Score=187, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI25147552, Length=317, Percent_Identity=38.4858044164038, Blast_Score=174, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17561716, Length=119, Percent_Identity=46.218487394958, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71996324, Length=317, Percent_Identity=25.5520504731861, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=313, Percent_Identity=40.5750798722045, Blast_Score=198, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6321449, Length=327, Percent_Identity=34.5565749235474, Blast_Score=142, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24643623, Length=311, Percent_Identity=39.5498392282958, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI20129101, Length=311, Percent_Identity=39.5498392282958, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32558; Mature: 32558

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYTSISELVGKTPLLEPRNYAQAHRLNARLLLKLDHLNPAGSMKDRIALAMINEAEAS
CCHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEEEEEECCCCCCCCHHEEEEEEEECCCCC
GQLKSGSVIIEPTSGNTGIGLAAIAASRGYRIILTMPESMSVERRNILKAYGADIVLTED
CCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEECC
SQGMKGAIAKAQDLAATTPGSFIPSQFTNPTNPRAHYETTGVEIWEDTDGEVDIVVAGVG
CCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCEEEECCEEEEECCCCCEEEEEEECC
TGGTITGIGRLLKERKPGGITVIAVEPADSPVLSAGQAGSHQIQGIGAGFIPETLDTDIY
CCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHH
DEVITVSNEDAFSTAKELGVKEGILTGISSGAALWAAVQVAKRKENTGKNVVVILPDSAD
HHEEEECCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCC
RYYSTPLFI
CEECCCCCC
>Mature Secondary Structure
MNIYTSISELVGKTPLLEPRNYAQAHRLNARLLLKLDHLNPAGSMKDRIALAMINEAEAS
CCHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEEEEEECCCCCCCCHHEEEEEEEECCCCC
GQLKSGSVIIEPTSGNTGIGLAAIAASRGYRIILTMPESMSVERRNILKAYGADIVLTED
CCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEECC
SQGMKGAIAKAQDLAATTPGSFIPSQFTNPTNPRAHYETTGVEIWEDTDGEVDIVVAGVG
CCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCEEEECCEEEEECCCCCEEEEEEECC
TGGTITGIGRLLKERKPGGITVIAVEPADSPVLSAGQAGSHQIQGIGAGFIPETLDTDIY
CCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHH
DEVITVSNEDAFSTAKELGVKEGILTGISSGAALWAAVQVAKRKENTGKNVVVILPDSAD
HHEEEECCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCC
RYYSTPLFI
CEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]