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The map label for this gene is cysD

Identifier: 187735792

GI number: 187735792

Start: 1575725

End: 1576636

Strand: Reverse

Name: cysD

Synonym: Amuc_1299

Alternate gene names: 187735792

Gene position: 1576636-1575725 (Counterclockwise)

Preceding gene: 187735793

Following gene: 187735791

Centisome position: 59.18

GC content: 47.15

Gene sequence:

>912_bases
ATGAACAGCATTTACAGACTCAGCCAGCTAAAACAGTTGGAGGCAGAAAGCATTCACATTTTCCGGGATGCCATCAGCCA
GTTTGAAAATCCCGTACTTCTTTATTCCATCGGGAAAGATTCCTCTGTACTGGTCCACTTAGCCAAAAAGGCATTCTATC
CAGGCAGACTCCCATTCAAGCTTCTTCATATAGACTCCACATTCAAATTTCGCGAGATGATAGAATTCCGGGACAGATTC
GTTCAGGAAAATGATCTGGATCTGATTGTCCACAGTAACCAGGAAGGCATTTCCGCCGGCGTCAATCCTTTTACTTACGG
CAGCCGCAAATACACGGATATTATGAAAACACAGGCATTGATTCAGGCGTTGACCGCAGGAAAATATGATGCCGTATTCG
GAGGCGCCCGCCGCGACGAAGAAAAATCACGGGCCAAAGAACGAATTTTCTCTTTCCGGGATAAATTCAACCAATGGGAT
CCCAAAAATCAACGGCCGGAATTATGGAACATTTATAACGGCCGTATCAATCCGGGAGAAAGCGTCCGTATTTTTCCTCT
GTCCAATTGGACGGAACTGGATATCTGGCAATACATCCGCCTGGAAAAAATACAGGTTGTAGACCTGTATTTTGCTAGAC
CCCGTCCCATTGTGGAACGTGACGGCTCCCTTATTATGGCGGACGACGACCGCCTGCCTTTGAAACCGGGGGAAACACCG
CAGATGAGAACCGTCCGTTTCCGTACACTGGGCTGTTACCCTCTAACTGGAGCCATAGAATCTACAGCTCGCACTGTAGA
AGAGATTGTAGCGGAAATGCTGGAAACCAGGCTGAGCGAGCGTTCTTCTCGTATAATTGACCACGACGGTGATGCCTCCA
TGGAACAGAAAAAACGAGAAGGATATTTTTAA

Upstream 100 bases:

>100_bases
AGAAGATGTACGCGACGGACGATGGTGGTGGGAAGAGAAAGAACACAAGGAGTGCGGTCTACACCGCAAACCAAGACATT
GATAACCAAAAATATACAAA

Downstream 100 bases:

>100_bases
CCCCTATTATGAACCATTCTGACGATACCTCTTATTTTTACCATTATGGACATCGACTCATACCTGAACGAACACGAAAA
TAAAAGCCTGCTACGCGTAC

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNSIYRLSQLKQLEAESIHIFRDAISQFENPVLLYSIGKDSSVLVHLAKKAFYPGRLPFKLLHIDSTFKFREMIEFRDRF
VQENDLDLIVHSNQEGISAGVNPFTYGSRKYTDIMKTQALIQALTAGKYDAVFGGARRDEEKSRAKERIFSFRDKFNQWD
PKNQRPELWNIYNGRINPGESVRIFPLSNWTELDIWQYIRLEKIQVVDLYFARPRPIVERDGSLIMADDDRLPLKPGETP
QMRTVRFRTLGCYPLTGAIESTARTVEEIVAEMLETRLSERSSRIIDHDGDASMEQKKREGYF

Sequences:

>Translated_303_residues
MNSIYRLSQLKQLEAESIHIFRDAISQFENPVLLYSIGKDSSVLVHLAKKAFYPGRLPFKLLHIDSTFKFREMIEFRDRF
VQENDLDLIVHSNQEGISAGVNPFTYGSRKYTDIMKTQALIQALTAGKYDAVFGGARRDEEKSRAKERIFSFRDKFNQWD
PKNQRPELWNIYNGRINPGESVRIFPLSNWTELDIWQYIRLEKIQVVDLYFARPRPIVERDGSLIMADDDRLPLKPGETP
QMRTVRFRTLGCYPLTGAIESTARTVEEIVAEMLETRLSERSSRIIDHDGDASMEQKKREGYF
>Mature_303_residues
MNSIYRLSQLKQLEAESIHIFRDAISQFENPVLLYSIGKDSSVLVHLAKKAFYPGRLPFKLLHIDSTFKFREMIEFRDRF
VQENDLDLIVHSNQEGISAGVNPFTYGSRKYTDIMKTQALIQALTAGKYDAVFGGARRDEEKSRAKERIFSFRDKFNQWD
PKNQRPELWNIYNGRINPGESVRIFPLSNWTELDIWQYIRLEKIQVVDLYFARPRPIVERDGSLIMADDDRLPLKPGETP
QMRTVRFRTLGCYPLTGAIESTARTVEEIVAEMLETRLSERSSRIIDHDGDASMEQKKREGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=298, Percent_Identity=65.4362416107383, Blast_Score=424, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_AKKM8 (B2URN8)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877904.1
- ProteinModelPortal:   B2URN8
- SMR:   B2URN8
- GeneID:   6275588
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_1299
- HOGENOM:   HBG758022
- OMA:   HYGFDAA
- ProtClustDB:   PRK05253
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 35311; Mature: 35311

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIYRLSQLKQLEAESIHIFRDAISQFENPVLLYSIGKDSSVLVHLAKKAFYPGRLPFK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEE
LLHIDSTFKFREMIEFRDRFVQENDLDLIVHSNQEGISAGVNPFTYGSRKYTDIMKTQAL
EEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHCCCCCCCCCCCHHHHHHHHHHH
IQALTAGKYDAVFGGARRDEEKSRAKERIFSFRDKFNQWDPKNQRPELWNIYNGRINPGE
HHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCC
SVRIFPLSNWTELDIWQYIRLEKIQVVDLYFARPRPIVERDGSLIMADDDRLPLKPGETP
CEEEEECCCCCCCHHHHHHHHCEEEEEEEEECCCCCHHCCCCCEEEECCCCCCCCCCCCC
QMRTVRFRTLGCYPLTGAIESTARTVEEIVAEMLETRLSERSSRIIDHDGDASMEQKKRE
CEEEEEEEEECCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHC
GYF
CCC
>Mature Secondary Structure
MNSIYRLSQLKQLEAESIHIFRDAISQFENPVLLYSIGKDSSVLVHLAKKAFYPGRLPFK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEE
LLHIDSTFKFREMIEFRDRFVQENDLDLIVHSNQEGISAGVNPFTYGSRKYTDIMKTQAL
EEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHCCCCCCCCCCCHHHHHHHHHHH
IQALTAGKYDAVFGGARRDEEKSRAKERIFSFRDKFNQWDPKNQRPELWNIYNGRINPGE
HHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCC
SVRIFPLSNWTELDIWQYIRLEKIQVVDLYFARPRPIVERDGSLIMADDDRLPLKPGETP
CEEEEECCCCCCCHHHHHHHHCEEEEEEEEECCCCCHHCCCCCEEEECCCCCCCCCCCCC
QMRTVRFRTLGCYPLTGAIESTARTVEEIVAEMLETRLSERSSRIIDHDGDASMEQKKRE
CEEEEEEEEECCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHC
GYF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA