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The map label for this gene is pdxJ

Identifier: 187735701

GI number: 187735701

Start: 1443246

End: 1443986

Strand: Direct

Name: pdxJ

Synonym: Amuc_1207

Alternate gene names: 187735701

Gene position: 1443246-1443986 (Clockwise)

Preceding gene: 187735700

Following gene: 187735702

Centisome position: 54.17

GC content: 59.78

Gene sequence:

>741_bases
ATGCTGCTAGGTGTAAATATAGACCACATCGCCACGTTGAGGCAGGCCCGCTACGCGACCATGCTGGATTCCTTCAATGT
GGAGCCGAGTGTTTTGGACGCCGCTTATGCGGCGCAAAGGGGCGGGGCGGATTCCATCACCCTCCATGTCCGGGGCGACC
GCCGCCACATGCAGGATGCGGATGCCCTGAGCGTCCGGGAGAGCGTGGCCCTTCCCCTGAACCTGGAAATGGGAAATACC
CCGGAGATGGTGGATTTTGCCCTGCGGCTGAAACCGGACTATATCTGCATGGTTCCGGAGAAGCGCGAGGAAATCACTAC
GGAAGGCGGCCTGGACGCCGTTTTTCATGAAAAGGATCTGGCTCCCACCATGGCAAGAATGGCCGACAACGGCATTCAGG
TGAGCCTGTTCATTGATCCGGAAGTGCCCCAGGTGGAGGCTGCCGCCCGCCTGGGCGCCCCCATGATTGAGTTGCATACC
GGATGTTTTGCCAACCATTCCGGCAGGGAGCGCACGGAGGAACTGGCCCGTTTGAAACGCGCCGCGGAACTGGCCCATTC
CCTGGGTATCCAGGTGAATGCGGGCCATGGCATCAATTACCAGAATCTGGAACAGCTGCTGGCTGGCGTCCCTTACCTGC
ATGAGCTGAACATCGGCCATACGATTGTATCCCGCGCTCTTTTTGTGGGGATGGAGCAGGCCGTGAGGGAAATGCGCCAG
GCGATCGACCGCCTGAGCTGA

Upstream 100 bases:

>100_bases
CGCCGCTGATGCCGCGGGAATTGCCATGGTATTTACGGGACACCGCCATTTCCTCCATTAATTTGAATGTTCGTCCCCGC
CCCTTTGTCTTAAAACCGAT

Downstream 100 bases:

>100_bases
TTGCGCCGTTTCAATTGATCCCGGTTGCCGGAGTATGAGCCGCATTGCAGGATTGGGAATGGATTTGGCGGATATGGAAC
GCGTACGCCAGGCCCTGCGG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MLLGVNIDHIATLRQARYATMLDSFNVEPSVLDAAYAAQRGGADSITLHVRGDRRHMQDADALSVRESVALPLNLEMGNT
PEMVDFALRLKPDYICMVPEKREEITTEGGLDAVFHEKDLAPTMARMADNGIQVSLFIDPEVPQVEAAARLGAPMIELHT
GCFANHSGRERTEELARLKRAAELAHSLGIQVNAGHGINYQNLEQLLAGVPYLHELNIGHTIVSRALFVGMEQAVREMRQ
AIDRLS

Sequences:

>Translated_246_residues
MLLGVNIDHIATLRQARYATMLDSFNVEPSVLDAAYAAQRGGADSITLHVRGDRRHMQDADALSVRESVALPLNLEMGNT
PEMVDFALRLKPDYICMVPEKREEITTEGGLDAVFHEKDLAPTMARMADNGIQVSLFIDPEVPQVEAAARLGAPMIELHT
GCFANHSGRERTEELARLKRAAELAHSLGIQVNAGHGINYQNLEQLLAGVPYLHELNIGHTIVSRALFVGMEQAVREMRQ
AIDRLS
>Mature_246_residues
MLLGVNIDHIATLRQARYATMLDSFNVEPSVLDAAYAAQRGGADSITLHVRGDRRHMQDADALSVRESVALPLNLEMGNT
PEMVDFALRLKPDYICMVPEKREEITTEGGLDAVFHEKDLAPTMARMADNGIQVSLFIDPEVPQVEAAARLGAPMIELHT
GCFANHSGRERTEELARLKRAAELAHSLGIQVNAGHGINYQNLEQLLAGVPYLHELNIGHTIVSRALFVGMEQAVREMRQ
AIDRLS

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=241, Percent_Identity=49.3775933609959, Blast_Score=239, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_AKKM8 (B2URE7)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877813.1
- ProteinModelPortal:   B2URE7
- SMR:   B2URE7
- GeneID:   6273747
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_1207
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 27114; Mature: 27114

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: ACT_SITE 49-49 ACT_SITE 76-76 ACT_SITE 196-196 BINDING 6-6 BINDING 17-17 BINDING 51-51 BINDING 56-56 BINDING 106-106 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLGVNIDHIATLRQARYATMLDSFNVEPSVLDAAYAAQRGGADSITLHVRGDRRHMQDA
CEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHCCC
DALSVRESVALPLNLEMGNTPEMVDFALRLKPDYICMVPEKREEITTEGGLDAVFHEKDL
HHHHHHHHEECEEEECCCCCCHHHHHHEEECCCEEEECCCHHHHHCCCCCCCHHHCCCCC
APTMARMADNGIQVSLFIDPEVPQVEAAARLGAPMIELHTGCFANHSGRERTEELARLKR
HHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCEEHHCCCCCCCCCHHHHHHHHHHHH
AAELAHSLGIQVNAGHGINYQNLEQLLAGVPYLHELNIGHTIVSRALFVGMEQAVREMRQ
HHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
AIDRLS
HHHHCC
>Mature Secondary Structure
MLLGVNIDHIATLRQARYATMLDSFNVEPSVLDAAYAAQRGGADSITLHVRGDRRHMQDA
CEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHCCC
DALSVRESVALPLNLEMGNTPEMVDFALRLKPDYICMVPEKREEITTEGGLDAVFHEKDL
HHHHHHHHEECEEEECCCCCCHHHHHHEEECCCEEEECCCHHHHHCCCCCCCHHHCCCCC
APTMARMADNGIQVSLFIDPEVPQVEAAARLGAPMIELHTGCFANHSGRERTEELARLKR
HHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCEEHHCCCCCCCCCHHHHHHHHHHHH
AAELAHSLGIQVNAGHGINYQNLEQLLAGVPYLHELNIGHTIVSRALFVGMEQAVREMRQ
HHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
AIDRLS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA