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The map label for this gene is eno [H]

Identifier: 187735678

GI number: 187735678

Start: 1418697

End: 1419977

Strand: Reverse

Name: eno [H]

Synonym: Amuc_1184

Alternate gene names: 187735678

Gene position: 1419977-1418697 (Counterclockwise)

Preceding gene: 187735682

Following gene: 187735677

Centisome position: 53.3

GC content: 56.13

Gene sequence:

>1281_bases
ATGGAAATCATTGACGTCCGCGGCCGTGAAATCATCGACTCACGGGGCAATCCCACCGTGGAAGTGGACGTGGCCCTCGC
CGGGGGAGCTATCGGACGCGCATCCGTCCCCAGCGGAGCCTCCACCGGAGAACATGAAGCCTGGGAACTCCGGGATGGAG
ACGCCAAGCGCTACGGAGGCAAGGGCGTGCTTAAAGCCGTGGAAAACATCAATAAAATCATTGCCCCGGAAATATCAGGG
CACGATGCCACCCTGCAGCCGGCCATTGACAAAATCATGATCGACCTGGACGGCACGCCCAATAAATCCCGCCTGGGGGC
CAACGCCATCCTGGGCGTCTCCCTGGCCGTGGCAAAAGCCGCCGCCATTCAGCTCAACCTTCCCCTTTTCAAATACCTGG
GTGGACCGAACGCCAAGGTTTTGCCCGTCCCCATGATGAACATAATCAATGGTGGAGCCCATTCGGACTCCCCCATCGAC
TTTCAGGAATTCATGATCATGCCCAAGGGGGCTCCCACTTTCCGGGAATCCCTGCGCTACGGGGCGGAAGTTTTCCATGC
GCTCAAGGATGTGCTGCATGACCGCGGCCTTTCCACTGCTGTGGGGGATGAAGGGGGATTCGCCCCGGCCCTGAAATCAG
CAGACGACGCCCTGGAATGCATTGCACAGGCCGTAAAACGGGCCGGGTACACCCTGGGCACGGATATCTTCATTGCCCTG
GACGTGGCTTCCTCCGAATTCTACGATCCCTCCCGGAACCTGTACGTCTTCAAAAAATCAGACGGCCTGGGCAGAACAGC
GGAGGAACTGACGGCTTACTATCAGGAACTCCAGAAAAAATACCCCATCATTTCCATTGAGGACGGCTGTGCGGAAAACG
ACTGGCTGGGCTGGGAACAGCTAACTAAAGCCATGGGCGGCAATACCCAGCTGGTAGGGGACGACCTGTTCGTGACGAAT
GTGGAATTCCTGAATCAGGGCATTTCCCGCCATGTAGCGAATGCCGTCCTGGTGAAAGTCAACCAGATAGGTTCCCTGAC
GGAAACGCTGGATACGGTGGAACTGGCCAAGGATAACAAATACTCCGCCATCATCTCCCACCGCTCCGGGGAAACGGAAG
ACGCCACGATCGCGGATATTGCCGTAGCGACGAACGCGGGGCAAATCAAAACCGGCTCTCTAAGCCGTTCCGACCGCATG
GCCAAATACAATCAATTGCTTAGAATTGAAGAAGAACTGGGAAATGACGCAGTTTATGGTGGTAAAATTCATATTCTATG
A

Upstream 100 bases:

>100_bases
GAAACCGGAACTTCTAGGGACATGACATGCTTCTCCGGAACGGGAAAGCCTGTTTCCCTGTCTAAAAAGGACAAAAAGAA
ATCTTATGATCATGAATGAA

Downstream 100 bases:

>100_bases
TCAAAAATATGTTGTTTGACTGCAAGGAATGCTCTGGAGACGCCGCTACTACCACCGTTTTACCCTGGCGGAGCTTGAAA
TCCGCCGGGAGAAACGTGCC

Product: Phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MEIIDVRGREIIDSRGNPTVEVDVALAGGAIGRASVPSGASTGEHEAWELRDGDAKRYGGKGVLKAVENINKIIAPEISG
HDATLQPAIDKIMIDLDGTPNKSRLGANAILGVSLAVAKAAAIQLNLPLFKYLGGPNAKVLPVPMMNIINGGAHSDSPID
FQEFMIMPKGAPTFRESLRYGAEVFHALKDVLHDRGLSTAVGDEGGFAPALKSADDALECIAQAVKRAGYTLGTDIFIAL
DVASSEFYDPSRNLYVFKKSDGLGRTAEELTAYYQELQKKYPIISIEDGCAENDWLGWEQLTKAMGGNTQLVGDDLFVTN
VEFLNQGISRHVANAVLVKVNQIGSLTETLDTVELAKDNKYSAIISHRSGETEDATIADIAVATNAGQIKTGSLSRSDRM
AKYNQLLRIEEELGNDAVYGGKIHIL

Sequences:

>Translated_426_residues
MEIIDVRGREIIDSRGNPTVEVDVALAGGAIGRASVPSGASTGEHEAWELRDGDAKRYGGKGVLKAVENINKIIAPEISG
HDATLQPAIDKIMIDLDGTPNKSRLGANAILGVSLAVAKAAAIQLNLPLFKYLGGPNAKVLPVPMMNIINGGAHSDSPID
FQEFMIMPKGAPTFRESLRYGAEVFHALKDVLHDRGLSTAVGDEGGFAPALKSADDALECIAQAVKRAGYTLGTDIFIAL
DVASSEFYDPSRNLYVFKKSDGLGRTAEELTAYYQELQKKYPIISIEDGCAENDWLGWEQLTKAMGGNTQLVGDDLFVTN
VEFLNQGISRHVANAVLVKVNQIGSLTETLDTVELAKDNKYSAIISHRSGETEDATIADIAVATNAGQIKTGSLSRSDRM
AKYNQLLRIEEELGNDAVYGGKIHIL
>Mature_426_residues
MEIIDVRGREIIDSRGNPTVEVDVALAGGAIGRASVPSGASTGEHEAWELRDGDAKRYGGKGVLKAVENINKIIAPEISG
HDATLQPAIDKIMIDLDGTPNKSRLGANAILGVSLAVAKAAAIQLNLPLFKYLGGPNAKVLPVPMMNIINGGAHSDSPID
FQEFMIMPKGAPTFRESLRYGAEVFHALKDVLHDRGLSTAVGDEGGFAPALKSADDALECIAQAVKRAGYTLGTDIFIAL
DVASSEFYDPSRNLYVFKKSDGLGRTAEELTAYYQELQKKYPIISIEDGCAENDWLGWEQLTKAMGGNTQLVGDDLFVTN
VEFLNQGISRHVANAVLVKVNQIGSLTETLDTVELAKDNKYSAIISHRSGETEDATIADIAVATNAGQIKTGSLSRSDRM
AKYNQLLRIEEELGNDAVYGGKIHIL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=50.9302325581395, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=50.1160092807425, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=49.7685185185185, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=49.7685185185185, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=44.6511627906977, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI169201331, Length=346, Percent_Identity=26.5895953757225, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI169201757, Length=346, Percent_Identity=26.5895953757225, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI239744207, Length=346, Percent_Identity=26.5895953757225, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1789141, Length=428, Percent_Identity=59.5794392523364, Blast_Score=489, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=52.4361948955916, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=52.4361948955916, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=49.2063492063492, Blast_Score=175, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=48.4988452655889, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=48.4988452655889, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=48.4988452655889, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321693, Length=436, Percent_Identity=48.8532110091743, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321968, Length=436, Percent_Identity=47.7064220183486, Blast_Score=345, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=50, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=50, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=50, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=50, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=50, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=50, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45683; Mature: 45683

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIIDVRGREIIDSRGNPTVEVDVALAGGAIGRASVPSGASTGEHEAWELRDGDAKRYGG
CCEEECCCCHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCHHCCC
KGVLKAVENINKIIAPEISGHDATLQPAIDKIMIDLDGTPNKSRLGANAILGVSLAVAKA
HHHHHHHHHHHHHCCCCCCCCCCCHHCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHH
AAIQLNLPLFKYLGGPNAKVLPVPMMNIINGGAHSDSPIDFQEFMIMPKGAPTFRESLRY
EEEEECCCHHHHCCCCCCEEEECCHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHHHHH
GAEVFHALKDVLHDRGLSTAVGDEGGFAPALKSADDALECIAQAVKRAGYTLGTDIFIAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCEECCEEEEEE
DVASSEFYDPSRNLYVFKKSDGLGRTAEELTAYYQELQKKYPIISIEDGCAENDWLGWEQ
EECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHH
LTKAMGGNTQLVGDDLFVTNVEFLNQGISRHVANAVLVKVNQIGSLTETLDTVELAKDNK
HHHHHCCCCEEECCCEEEEEHHHHHHHHHHHHHHHHEEEEHHHCCHHHHHHHHHHHCCCC
YSAIISHRSGETEDATIADIAVATNAGQIKTGSLSRSDRMAKYNQLLRIEEELGNDAVYG
EEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEC
GKIHIL
CEEEEC
>Mature Secondary Structure
MEIIDVRGREIIDSRGNPTVEVDVALAGGAIGRASVPSGASTGEHEAWELRDGDAKRYGG
CCEEECCCCHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCHHCCC
KGVLKAVENINKIIAPEISGHDATLQPAIDKIMIDLDGTPNKSRLGANAILGVSLAVAKA
HHHHHHHHHHHHHCCCCCCCCCCCHHCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHH
AAIQLNLPLFKYLGGPNAKVLPVPMMNIINGGAHSDSPIDFQEFMIMPKGAPTFRESLRY
EEEEECCCHHHHCCCCCCEEEECCHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHHHHH
GAEVFHALKDVLHDRGLSTAVGDEGGFAPALKSADDALECIAQAVKRAGYTLGTDIFIAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCEECCEEEEEE
DVASSEFYDPSRNLYVFKKSDGLGRTAEELTAYYQELQKKYPIISIEDGCAENDWLGWEQ
EECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHH
LTKAMGGNTQLVGDDLFVTNVEFLNQGISRHVANAVLVKVNQIGSLTETLDTVELAKDNK
HHHHHCCCCEEECCCEEEEEHHHHHHHHHHHHHHHHEEEEHHHCCHHHHHHHHHHHCCCC
YSAIISHRSGETEDATIADIAVATNAGQIKTGSLSRSDRMAKYNQLLRIEEELGNDAVYG
EEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEC
GKIHIL
CEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA