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The map label for this gene is glkA [H]

Identifier: 187735589

GI number: 187735589

Start: 1305302

End: 1306300

Strand: Direct

Name: glkA [H]

Synonym: Amuc_1094

Alternate gene names: 187735589

Gene position: 1305302-1306300 (Clockwise)

Preceding gene: 187735581

Following gene: 187735590

Centisome position: 49.0

GC content: 58.26

Gene sequence:

>999_bases
ATGAGCTTTTCAGAACCCTGTGCCTTGGCCGTTGATTTCGGCGGCACGAGCATCAAAATGGGCGTAACGGCGGGGGATCG
TATTCTGGCGACGGCCGACCGCATCCCTACTGCCATGTTCGAAAGCCCGCAGGCAATCATTGATGCCATGATTGCGTCCG
CCCGCACCCTGCGCGGACAATTCCCCTCCGCCTGTGTGATGGGCATGGGAATGCCGGGATGGTGTGATTACCAGCGGGGA
GTGCTTTACCAGCTTACCAATGTGAGGGTCTGGGATAGGGAAATTCCGGTGAAAGAGATGATGGAGCAGGCCCTGGGCCT
CCCCGTCGTGCTGGATAATGACGCCAACTGCATGGCTTATGCGGAATGGAAGCTTGGCGCCGGGCGCGGCATGTCCAGCC
TGGTGTGCCTGACGATGGGAACGGGGATAGGCGGGGGAATCGTGGTGCATGACCGCATGCTGCGGGGAAGGCGGCTTTCC
GCTGCGGAACTGGGCCAGACCAGCATTCATTACCAGGGGAAAACGGGACCGTTCGGCAGCCGGGGAGCCATTGAGGAATA
CATCGGCAACAACGAACTGGCGGCGGAGGCGGTTAAACGGTATGCCGGGGCGGGAATCATCAAGACGGTGGATGAATGCA
CGCCCAGGCATCTGGACGAGGCTGCCCGGTCCGGATGTCCTATAGCCCTTCAATTATGGGAAGATACGGCGGAAATGCTG
GGCTGCCTGATCATGAACCTGATGTATACGCTGGTGCCGGACGCCTTCATCATCGGGGGCGGTGTGGCCAAGGCAGGGGA
TTTGTTGATGAAGCCGCTGCTGGAGAACCTCAGGAAACAGTTGTTTCCTCTCCTGATGGAGGATTTGAAAATTCTGCCTG
CCAGATTTGGAGCGGAGGCGGGGTTGCTGGGAGCGGGAGCCATGGCGATGGATGAATTCATGGGGCTGGGGATTTTGGAA
CGGTTTAAGAACCAGAAATCAACGCAGACTTTTTGTTAG

Upstream 100 bases:

>100_bases
TATTGTGTTTTATACGGTAAGTGCGCCATGCCGCGGAAAGGGGTGGGGATGAATGCGTTTTTACGTTGATTCCGGAGAAT
GGGTGGCATAAGTATGGACT

Downstream 100 bases:

>100_bases
TTTGGGCTAATATAGGGCTTGCCATGCCGGAGAGGCAGGCGTACAAGCGGAACAGGTCATGAGCACGGAACATTCCCACG
AACATTTTCCGGAAGGTGCT

Product: ROK family protein

Products: NA

Alternate protein names: Glucose kinase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSFSEPCALAVDFGGTSIKMGVTAGDRILATADRIPTAMFESPQAIIDAMIASARTLRGQFPSACVMGMGMPGWCDYQRG
VLYQLTNVRVWDREIPVKEMMEQALGLPVVLDNDANCMAYAEWKLGAGRGMSSLVCLTMGTGIGGGIVVHDRMLRGRRLS
AAELGQTSIHYQGKTGPFGSRGAIEEYIGNNELAAEAVKRYAGAGIIKTVDECTPRHLDEAARSGCPIALQLWEDTAEML
GCLIMNLMYTLVPDAFIIGGGVAKAGDLLMKPLLENLRKQLFPLLMEDLKILPARFGAEAGLLGAGAMAMDEFMGLGILE
RFKNQKSTQTFC

Sequences:

>Translated_332_residues
MSFSEPCALAVDFGGTSIKMGVTAGDRILATADRIPTAMFESPQAIIDAMIASARTLRGQFPSACVMGMGMPGWCDYQRG
VLYQLTNVRVWDREIPVKEMMEQALGLPVVLDNDANCMAYAEWKLGAGRGMSSLVCLTMGTGIGGGIVVHDRMLRGRRLS
AAELGQTSIHYQGKTGPFGSRGAIEEYIGNNELAAEAVKRYAGAGIIKTVDECTPRHLDEAARSGCPIALQLWEDTAEML
GCLIMNLMYTLVPDAFIIGGGVAKAGDLLMKPLLENLRKQLFPLLMEDLKILPARFGAEAGLLGAGAMAMDEFMGLGILE
RFKNQKSTQTFC
>Mature_331_residues
SFSEPCALAVDFGGTSIKMGVTAGDRILATADRIPTAMFESPQAIIDAMIASARTLRGQFPSACVMGMGMPGWCDYQRGV
LYQLTNVRVWDREIPVKEMMEQALGLPVVLDNDANCMAYAEWKLGAGRGMSSLVCLTMGTGIGGGIVVHDRMLRGRRLSA
AELGQTSIHYQGKTGPFGSRGAIEEYIGNNELAAEAVKRYAGAGIIKTVDECTPRHLDEAARSGCPIALQLWEDTAEMLG
CLIMNLMYTLVPDAFIIGGGVAKAGDLLMKPLLENLRKQLFPLLMEDLKILPARFGAEAGLLGAGAMAMDEFMGLGILER
FKNQKSTQTFC

Specific function: Required for glucose repression of many different genes [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI190014632, Length=316, Percent_Identity=28.4810126582279, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI4885285, Length=316, Percent_Identity=28.4810126582279, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI298566315, Length=313, Percent_Identity=28.1150159744409, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI298566325, Length=313, Percent_Identity=28.1150159744409, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1787363, Length=315, Percent_Identity=26.6666666666667, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787878, Length=259, Percent_Identity=22.007722007722, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081733, Length=318, Percent_Identity=26.1006289308176, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR004654 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 35758; Mature: 35627

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSEPCALAVDFGGTSIKMGVTAGDRILATADRIPTAMFESPQAIIDAMIASARTLRGQ
CCCCCCCEEEEECCCCEEEEECCCCCCEEEEHHHCCHHHHCCHHHHHHHHHHHHHHHHCC
FPSACVMGMGMPGWCDYQRGVLYQLTNVRVWDREIPVKEMMEQALGLPVVLDNDANCMAY
CCCHHHHCCCCCCCCCCCCCCEEEECCEEEECCCCCHHHHHHHHCCCCEEECCCCCEEEE
AEWKLGAGRGMSSLVCLTMGTGIGGGIVVHDRMLRGRRLSAAELGQTSIHYQGKTGPFGS
EEEECCCCCCHHHEEEEEECCCCCCCEEEEHHHHCCCCCCHHHCCCCEEEECCCCCCCCC
RGAIEEYIGNNELAAEAVKRYAGAGIIKTVDECTPRHLDEAARSGCPIALQLWEDTAEML
CCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCEEEEHHHHHHHHH
GCLIMNLMYTLVPDAFIIGGGVAKAGDLLMKPLLENLRKQLFPLLMEDLKILPARFGAEA
HHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLLGAGAMAMDEFMGLGILERFKNQKSTQTFC
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SFSEPCALAVDFGGTSIKMGVTAGDRILATADRIPTAMFESPQAIIDAMIASARTLRGQ
CCCCCCEEEEECCCCEEEEECCCCCCEEEEHHHCCHHHHCCHHHHHHHHHHHHHHHHCC
FPSACVMGMGMPGWCDYQRGVLYQLTNVRVWDREIPVKEMMEQALGLPVVLDNDANCMAY
CCCHHHHCCCCCCCCCCCCCCEEEECCEEEECCCCCHHHHHHHHCCCCEEECCCCCEEEE
AEWKLGAGRGMSSLVCLTMGTGIGGGIVVHDRMLRGRRLSAAELGQTSIHYQGKTGPFGS
EEEECCCCCCHHHEEEEEECCCCCCCEEEEHHHHCCCCCCHHHCCCCEEEECCCCCCCCC
RGAIEEYIGNNELAAEAVKRYAGAGIIKTVDECTPRHLDEAARSGCPIALQLWEDTAEML
CCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCEEEEHHHHHHHHH
GCLIMNLMYTLVPDAFIIGGGVAKAGDLLMKPLLENLRKQLFPLLMEDLKILPARFGAEA
HHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLLGAGAMAMDEFMGLGILERFKNQKSTQTFC
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1435260; 12000953 [H]