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The map label for this gene is pdhC [H]

Identifier: 187735448

GI number: 187735448

Start: 1127732

End: 1128772

Strand: Reverse

Name: pdhC [H]

Synonym: Amuc_0947

Alternate gene names: 187735448

Gene position: 1128772-1127732 (Counterclockwise)

Preceding gene: 187735449

Following gene: 187735447

Centisome position: 42.37

GC content: 65.51

Gene sequence:

>1041_bases
ATGGCTATTACCATTGAAATGCCCCGCCTGTCCGACTCCATGCACGAAGGCACCGTCCTGAGATGGCTCAAAAAAACAGG
GGACTTCGTAAAAGTCGGCGACCATCTGGCCGACATTGAGACGGATAAGGCCCATGTGGAGCTCCAGGCCTGCGAGGACG
GCACCCTAACGGAAATTCTGGTGCCGGAGGGAGGTTCCGCCGCGGCGGGAGCGCCCATTGCGCTGCTTCAGCCGGAATTC
GGCGCAGCCGCCTGCGGCGGCCCCCCGCGCCCGTCCGCAACATGCAGCCCGCTGGCCGCCAGGCTGGCGGCGGAAGCCGG
CCTGAACCCCGCCACGCTGCGCGGCACCGGCCCCCGGGGCAAAATCATGGCCGCGGATGTACGGGCCGCCCTGCGCCCCG
CGGACGGCCCCGCCCGCCGGGTGCAGACCCCGCTGGCGCCCCGGGACACGCGCCACGCCACGCGCGTAGACAACTTTTAC
CTGTATTCCCTGGAGGCGAACATGGCTTACCTGGCCGCCATCAGCACGCCCATCGCCGTCCAGTGCGAAAAACTCATCGG
CGGCCGCTACAGCCTCTTTGACTACGTGGTGCGCGCGGCGGTGAAAGCATGCGCCAGCGAGCCGGAATGGCTGGCCGGAG
ACGCCGCCGCAGAACTGCTCATGGTGCTGGACAAGGGGGAAAAATACGTCTTCATTGAAAACGCCTCCGGCAAGACGATT
TACAACATCGCCATGGAACGCCTGGCCGCCCCCGCGGCAGGCCCTCTCCCCGCGGGCAGCGTCGCGCCCAACATCCTTCT
TTGCGACAGCGGCGTGAACGTGGCAGCCCAGCGCGCCGTGCTGCCGGAAATGCCCCACAGCATCATCAGCATAGGAGGCA
CCACGCCGAAAACGGGCATTGAAGCGGGGCGCCCCGTGTCCAAACTCATCCTGCCCGTCACCCTGTACGTGAACGCCGGA
ATTCTGCCGGAATGCAAGGCTTCCAACATTGCCGCCGAATTCAAAACCCTGCTGGAAAACCCGGTTCTTCTCCTCCTGTA
G

Upstream 100 bases:

>100_bases
AAAATACAGCGCGTAACCGCGTTTCCGCGCGCCGGAAGGAGACGTCCCGCCCCTTCCGGCGCGCCTTCCTCCCTTTTACC
ATATTTCAACATTCACCTCC

Downstream 100 bases:

>100_bases
GGGTACCGGAACATGCTGCTCTTTTCCTTGAACGTTCCTCTTCCTCCCCCTGTCTTACCAACCATGAAAAAAGTCGCCAT
ACTTGCCGCCCCCGGATTTG

Product: biotin/lipoyl attachment domain-containing protein

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MAITIEMPRLSDSMHEGTVLRWLKKTGDFVKVGDHLADIETDKAHVELQACEDGTLTEILVPEGGSAAAGAPIALLQPEF
GAAACGGPPRPSATCSPLAARLAAEAGLNPATLRGTGPRGKIMAADVRAALRPADGPARRVQTPLAPRDTRHATRVDNFY
LYSLEANMAYLAAISTPIAVQCEKLIGGRYSLFDYVVRAAVKACASEPEWLAGDAAAELLMVLDKGEKYVFIENASGKTI
YNIAMERLAAPAAGPLPAGSVAPNILLCDSGVNVAAQRAVLPEMPHSIISIGGTTPKTGIEAGRPVSKLILPVTLYVNAG
ILPECKASNIAAEFKTLLENPVLLLL

Sequences:

>Translated_346_residues
MAITIEMPRLSDSMHEGTVLRWLKKTGDFVKVGDHLADIETDKAHVELQACEDGTLTEILVPEGGSAAAGAPIALLQPEF
GAAACGGPPRPSATCSPLAARLAAEAGLNPATLRGTGPRGKIMAADVRAALRPADGPARRVQTPLAPRDTRHATRVDNFY
LYSLEANMAYLAAISTPIAVQCEKLIGGRYSLFDYVVRAAVKACASEPEWLAGDAAAELLMVLDKGEKYVFIENASGKTI
YNIAMERLAAPAAGPLPAGSVAPNILLCDSGVNVAAQRAVLPEMPHSIISIGGTTPKTGIEAGRPVSKLILPVTLYVNAG
ILPECKASNIAAEFKTLLENPVLLLL
>Mature_345_residues
AITIEMPRLSDSMHEGTVLRWLKKTGDFVKVGDHLADIETDKAHVELQACEDGTLTEILVPEGGSAAAGAPIALLQPEFG
AAACGGPPRPSATCSPLAARLAAEAGLNPATLRGTGPRGKIMAADVRAALRPADGPARRVQTPLAPRDTRHATRVDNFYL
YSLEANMAYLAAISTPIAVQCEKLIGGRYSLFDYVVRAAVKACASEPEWLAGDAAAELLMVLDKGEKYVFIENASGKTIY
NIAMERLAAPAAGPLPAGSVAPNILLCDSGVNVAAQRAVLPEMPHSIISIGGTTPKTGIEAGRPVSKLILPVTLYVNAGI
LPECKASNIAAEFKTLLENPVLLLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=439, Percent_Identity=24.8291571753986, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI203098816, Length=62, Percent_Identity=54.8387096774194, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI203098753, Length=62, Percent_Identity=54.8387096774194, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25146366, Length=95, Percent_Identity=37.8947368421053, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 36247; Mature: 36116

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITIEMPRLSDSMHEGTVLRWLKKTGDFVKVGDHLADIETDKAHVELQACEDGTLTEIL
CEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCHHHCCCCCCEEEEEEECCCCCEEEEE
VPEGGSAAAGAPIALLQPEFGAAACGGPPRPSATCSPLAARLAAEAGLNPATLRGTGPRG
ECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCC
KIMAADVRAALRPADGPARRVQTPLAPRDTRHATRVDNFYLYSLEANMAYLAAISTPIAV
CEEEECHHHHHCCCCCCHHHCCCCCCCCCCCCHHCCCCEEEEEEECCEEEEEECCCCHHE
QCEKLIGGRYSLFDYVVRAAVKACASEPEWLAGDAAAELLMVLDKGEKYVFIENASGKTI
EHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEECCCEEEEEECCCCCCH
YNIAMERLAAPAAGPLPAGSVAPNILLCDSGVNVAAQRAVLPEMPHSIISIGGTTPKTGI
HHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCHHHEEECCCCCCCCC
EAGRPVSKLILPVTLYVNAGILPECKASNIAAEFKTLLENPVLLLL
CCCCCHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
AITIEMPRLSDSMHEGTVLRWLKKTGDFVKVGDHLADIETDKAHVELQACEDGTLTEIL
EEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCHHHCCCCCCEEEEEEECCCCCEEEEE
VPEGGSAAAGAPIALLQPEFGAAACGGPPRPSATCSPLAARLAAEAGLNPATLRGTGPRG
ECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCC
KIMAADVRAALRPADGPARRVQTPLAPRDTRHATRVDNFYLYSLEANMAYLAAISTPIAV
CEEEECHHHHHCCCCCCHHHCCCCCCCCCCCCHHCCCCEEEEEEECCEEEEEECCCCHHE
QCEKLIGGRYSLFDYVVRAAVKACASEPEWLAGDAAAELLMVLDKGEKYVFIENASGKTI
EHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEECCCEEEEEECCCCCCH
YNIAMERLAAPAAGPLPAGSVAPNILLCDSGVNVAAQRAVLPEMPHSIISIGGTTPKTGI
HHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCHHHEEECCCCCCCCC
EAGRPVSKLILPVTLYVNAGILPECKASNIAAEFKTLLENPVLLLL
CCCCCHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]