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The map label for this gene is zraS [H]

Identifier: 187735360

GI number: 187735360

Start: 1022954

End: 1024396

Strand: Reverse

Name: zraS [H]

Synonym: Amuc_0858

Alternate gene names: 187735360

Gene position: 1024396-1022954 (Counterclockwise)

Preceding gene: 187735361

Following gene: 187735359

Centisome position: 38.45

GC content: 53.92

Gene sequence:

>1443_bases
ATGATCATTGAACAGGAAGAATTCCAGAAAAAACTAAAACGCATCAATCTGGCGTACTTCTCCGATCCGGACCGCATTAT
TACCATCAGCAGGGGAAACAGCGTCGTCCGCCAGGGGGAACAGAACAAAAGGCTTTACCTGATTTTATCCGGCAGCGTGG
TTTCCTACCGCCACGTCGTTAAAGAAGACCCGGAAACAGCTTCCACTTCCCACAAAATCTACAAGGCGTTCCGGGCCGGT
CCCGGAGACTATTTGGGAGTGCAGAGTTTCTTTTCCGGATGGTTCCGCAGCTCCTCCGATCTCATTGCGGAAACTGATTT
GGAACTGGCCTATATCGACACCGCCACCCCTGCCGTGGACGTGGAACACTACGGCACGCTCCTGGAACAGTTTGTTCCCG
CCATTGTCCATGAACTGGCGGCGCGCAATACCCGCGTCTTTGACCGCACTGCGGAAAAGGAAGAAGCGCTGCGGATGCTC
CACCGTTCTGAAATGGCCGCCACCCTGGGGCAATTATCCGCTGGAATAGCCCATGAGCTCAACAATTCCCTGGGAGTCCT
GTCCCGTAAAACAGACTTTGTTCTGGAAACCCTGGACAACATGTTCCGCCAGGAAAACGCCTTCCGCTACACCCTGTACA
ACAAAGGGTTGGAAGGGGCTTCCTCCTCATCCACTTCCGAACAGCGCAGGCTGGCGCGCCAGTTTGAAAAAAATTACGGG
CTTTCCCAGGACGTGGCCAAGGCCCTGGCCCTGATTCCGGACATACAGAATCAGCCGGAGCTGCTGAACAAGCCATTCCT
CAGCCAGCTGGAACAAAACCTCCCTTATTGGGAATTGGGACACGACCTGCACGATATGAAACTGGCTGTCAGGCATGCTA
CAGCCATTGTCCGTTCCGTTCGCCTGCTGGGAGGAGGCAACACCAGACGGGAAGAAGGCGTAAACGTGACGGACTCCCTT
ACGGAAGCTGTTGCTCTGGTCAAGTCCAGTCTGCGAGGCGTTGATTTTGAAATGGTCCTGCCTCCCAGAGACTCCGTGCC
CGGCATCTTCGCAGATATGACGGAGCTCGTTCAAATTTGGATCAACCTGCTTCAAAACGCCTGCGACGCGCTGAAAATAG
AAAATACCCCCAACCCTTCCATCCACGTAGAATGCCGGGTGGAAAAAATCAGCAGCAAGGACCAGACATCCCATACCACC
CGTGAAATTCTGGTCTCCGTCACAGACAATGGCCCCGGCGTCCCAAAGGACCTGCATGAAAAAATTTTCCGTCCCAACTT
CACTACCAAAAAGAAAGGTCTTTCCTTCGGCCTGGGGCTGGGGCTCACCATCGTCCGGCGCATTGTGGACAGCTACGGCG
GCCGCATCCGCCTGGAGAGCGTTCCGGGCCATACCGTTTTTACGATAGTCATACCAACCATCAACAATCATGGAAAAAAT
TAA

Upstream 100 bases:

>100_bases
ATGATGATTCTTGCCCAATGCCATGCTTTTTAAGTAAGGTAATGTTCCATGCCGGGACATGTTGCCCCGGCATGGCCAAC
ATCAACGGGAAAAGCGCCCT

Downstream 100 bases:

>100_bases
CATCATTTGCGTGGACGACCAGCCGGAAGTCCTGGACAGCGTTTTGCGCGACCTTCGTCCGCTGAATTCCTGCTTCCGCC
TGGAAGGGGTGGAATCCGCA

Product: cyclic nucleotide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MIIEQEEFQKKLKRINLAYFSDPDRIITISRGNSVVRQGEQNKRLYLILSGSVVSYRHVVKEDPETASTSHKIYKAFRAG
PGDYLGVQSFFSGWFRSSSDLIAETDLELAYIDTATPAVDVEHYGTLLEQFVPAIVHELAARNTRVFDRTAEKEEALRML
HRSEMAATLGQLSAGIAHELNNSLGVLSRKTDFVLETLDNMFRQENAFRYTLYNKGLEGASSSSTSEQRRLARQFEKNYG
LSQDVAKALALIPDIQNQPELLNKPFLSQLEQNLPYWELGHDLHDMKLAVRHATAIVRSVRLLGGGNTRREEGVNVTDSL
TEAVALVKSSLRGVDFEMVLPPRDSVPGIFADMTELVQIWINLLQNACDALKIENTPNPSIHVECRVEKISSKDQTSHTT
REILVSVTDNGPGVPKDLHEKIFRPNFTTKKKGLSFGLGLGLTIVRRIVDSYGGRIRLESVPGHTVFTIVIPTINNHGKN

Sequences:

>Translated_480_residues
MIIEQEEFQKKLKRINLAYFSDPDRIITISRGNSVVRQGEQNKRLYLILSGSVVSYRHVVKEDPETASTSHKIYKAFRAG
PGDYLGVQSFFSGWFRSSSDLIAETDLELAYIDTATPAVDVEHYGTLLEQFVPAIVHELAARNTRVFDRTAEKEEALRML
HRSEMAATLGQLSAGIAHELNNSLGVLSRKTDFVLETLDNMFRQENAFRYTLYNKGLEGASSSSTSEQRRLARQFEKNYG
LSQDVAKALALIPDIQNQPELLNKPFLSQLEQNLPYWELGHDLHDMKLAVRHATAIVRSVRLLGGGNTRREEGVNVTDSL
TEAVALVKSSLRGVDFEMVLPPRDSVPGIFADMTELVQIWINLLQNACDALKIENTPNPSIHVECRVEKISSKDQTSHTT
REILVSVTDNGPGVPKDLHEKIFRPNFTTKKKGLSFGLGLGLTIVRRIVDSYGGRIRLESVPGHTVFTIVIPTINNHGKN
>Mature_480_residues
MIIEQEEFQKKLKRINLAYFSDPDRIITISRGNSVVRQGEQNKRLYLILSGSVVSYRHVVKEDPETASTSHKIYKAFRAG
PGDYLGVQSFFSGWFRSSSDLIAETDLELAYIDTATPAVDVEHYGTLLEQFVPAIVHELAARNTRVFDRTAEKEEALRML
HRSEMAATLGQLSAGIAHELNNSLGVLSRKTDFVLETLDNMFRQENAFRYTLYNKGLEGASSSSTSEQRRLARQFEKNYG
LSQDVAKALALIPDIQNQPELLNKPFLSQLEQNLPYWELGHDLHDMKLAVRHATAIVRSVRLLGGGNTRREEGVNVTDSL
TEAVALVKSSLRGVDFEMVLPPRDSVPGIFADMTELVQIWINLLQNACDALKIENTPNPSIHVECRVEKISSKDQTSHTT
REILVSVTDNGPGVPKDLHEKIFRPNFTTKKKGLSFGLGLGLTIVRRIVDSYGGRIRLESVPGHTVFTIVIPTINNHGKN

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=131, Percent_Identity=32.0610687022901, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788549, Length=131, Percent_Identity=35.1145038167939, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790436, Length=163, Percent_Identity=28.2208588957055, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53740; Mature: 53740

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50042 CNMP_BINDING_3 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIEQEEFQKKLKRINLAYFSDPDRIITISRGNSVVRQGEQNKRLYLILSGSVVSYRHVV
CCCCHHHHHHHHHHCCCEEECCCCCEEEEECCCHHHHCCCCCCEEEEEECCCHHHHHHHH
KEDPETASTSHKIYKAFRAGPGDYLGVQSFFSGWFRSSSDLIAETDLELAYIDTATPAVD
HCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCC
VEHYGTLLEQFVPAIVHELAARNTRVFDRTAEKEEALRMLHRSEMAATLGQLSAGIAHEL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNSLGVLSRKTDFVLETLDNMFRQENAFRYTLYNKGLEGASSSSTSEQRRLARQFEKNYG
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
LSQDVAKALALIPDIQNQPELLNKPFLSQLEQNLPYWELGHDLHDMKLAVRHATAIVRSV
CCHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHH
RLLGGGNTRREEGVNVTDSLTEAVALVKSSLRGVDFEMVLPPRDSVPGIFADMTELVQIW
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHH
INLLQNACDALKIENTPNPSIHVECRVEKISSKDQTSHTTREILVSVTDNGPGVPKDLHE
HHHHHHHHHHEEECCCCCCCEEEEEEEEECCCCCCCHHHHHHEEEEECCCCCCCCHHHHH
KIFRPNFTTKKKGLSFGLGLGLTIVRRIVDSYGGRIRLESVPGHTVFTIVIPTINNHGKN
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure
MIIEQEEFQKKLKRINLAYFSDPDRIITISRGNSVVRQGEQNKRLYLILSGSVVSYRHVV
CCCCHHHHHHHHHHCCCEEECCCCCEEEEECCCHHHHCCCCCCEEEEEECCCHHHHHHHH
KEDPETASTSHKIYKAFRAGPGDYLGVQSFFSGWFRSSSDLIAETDLELAYIDTATPAVD
HCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCC
VEHYGTLLEQFVPAIVHELAARNTRVFDRTAEKEEALRMLHRSEMAATLGQLSAGIAHEL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNSLGVLSRKTDFVLETLDNMFRQENAFRYTLYNKGLEGASSSSTSEQRRLARQFEKNYG
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
LSQDVAKALALIPDIQNQPELLNKPFLSQLEQNLPYWELGHDLHDMKLAVRHATAIVRSV
CCHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHH
RLLGGGNTRREEGVNVTDSLTEAVALVKSSLRGVDFEMVLPPRDSVPGIFADMTELVQIW
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHH
INLLQNACDALKIENTPNPSIHVECRVEKISSKDQTSHTTREILVSVTDNGPGVPKDLHE
HHHHHHHHHHEEECCCCCCCEEEEEEEEECCCCCCCHHHHHHEEEEECCCCCCCCHHHHH
KIFRPNFTTKKKGLSFGLGLGLTIVRRIVDSYGGRIRLESVPGHTVFTIVIPTINNHGKN
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]