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The map label for this gene is vsr [H]

Identifier: 186476892

GI number: 186476892

Start: 2403657

End: 2404103

Strand: Direct

Name: vsr [H]

Synonym: Bphy_2141

Alternate gene names: 186476892

Gene position: 2403657-2404103 (Clockwise)

Preceding gene: 186476891

Following gene: 186476893

Centisome position: 69.09

GC content: 66.0

Gene sequence:

>447_bases
ATGGTCGACATTGTCGACGCGGTCACGCGCAGCCGGATGATGTCCGGCATTCGCGGCCGCAACACCAAGCCCGAAATCCT
GATCCGCAGCCTGCTTCACCGGCGCGGATTTCGTTTCCGGCTCGACGCACGCGATCTGCCCGGCCGGCCCGACATCGTGT
TGCCGCGCTATCGCGCCGTCGTCTTCATCCATGGCTGCTTCTGGCACGGTCACGACTGTCATCTCTTCAAATGGCCGCAG
ACACGGCCTGAATTCTGGCGCGACAAGATCGGCCGCAATCGCGCGAACGATGCGAAAGCGCAGGCCGCGCTGCTCGATAG
CGGCTGGCGCGTCGCGACCGTGTGGGAATGCGCCTTGCGCGGAGCGAACCGGGACATCGACGGCGTGCTGCAACGGCTCG
TCGATTGGCTGCACGGCGACGCGCCGCTGCACGAAGAGCGCGCCTGA

Upstream 100 bases:

>100_bases
TGGTGCCCGTCATGCGCGAGGTCGCGCGCATCATGCTGCCGCATGTGCTCGCGCTGACGCGCGCGGCGCACGACGCCAGC
GCTGCAGAACTGGCGGCCTG

Downstream 100 bases:

>100_bases
CATCGTGCCGCGCGACGGGACGGCAGGCTCGTGAGCGCCTTCGGCGCGGCGAACATTGCAACGCGCCAGTGCTACACTCG
ACTGGCTCACTGGAGCTTCG

Product: DNA mismatch endonuclease Vsr

Products: DNA [C]

Alternate protein names: DNA mismatch endonuclease; V.EcoKDcm; Vsr mismatch endonuclease [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MVDIVDAVTRSRMMSGIRGRNTKPEILIRSLLHRRGFRFRLDARDLPGRPDIVLPRYRAVVFIHGCFWHGHDCHLFKWPQ
TRPEFWRDKIGRNRANDAKAQAALLDSGWRVATVWECALRGANRDIDGVLQRLVDWLHGDAPLHEERA

Sequences:

>Translated_148_residues
MVDIVDAVTRSRMMSGIRGRNTKPEILIRSLLHRRGFRFRLDARDLPGRPDIVLPRYRAVVFIHGCFWHGHDCHLFKWPQ
TRPEFWRDKIGRNRANDAKAQAALLDSGWRVATVWECALRGANRDIDGVLQRLVDWLHGDAPLHEERA
>Mature_148_residues
MVDIVDAVTRSRMMSGIRGRNTKPEILIRSLLHRRGFRFRLDARDLPGRPDIVLPRYRAVVFIHGCFWHGHDCHLFKWPQ
TRPEFWRDKIGRNRANDAKAQAALLDSGWRVATVWECALRGANRDIDGVLQRLVDWLHGDAPLHEERA

Specific function: Deamination of 5-methylcytosine in DNA results in T/G mismatches. If unrepaired, these mismatches can lead to C-to-C transition mutations. The very short patch (VSP) repair process in E.coli counteracts the mutagenic process by repairing the mismatches in

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=143, Percent_Identity=48.2517482517483, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 17241; Mature: 17241

Theoretical pI: Translated: 11.00; Mature: 11.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDIVDAVTRSRMMSGIRGRNTKPEILIRSLLHRRGFRFRLDARDLPGRPDIVLPRYRAV
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEECCCCEEE
VFIHGCFWHGHDCHLFKWPQTRPEFWRDKIGRNRANDAKAQAALLDSGWRVATVWECALR
EEEEEEEECCCCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHC
GANRDIDGVLQRLVDWLHGDAPLHEERA
CCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVDIVDAVTRSRMMSGIRGRNTKPEILIRSLLHRRGFRFRLDARDLPGRPDIVLPRYRAV
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEECCCCEEE
VFIHGCFWHGHDCHLFKWPQTRPEFWRDKIGRNRANDAKAQAALLDSGWRVATVWECALR
EEEEEEEECCCCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHC
GANRDIDGVLQRLVDWLHGDAPLHEERA
CCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2527357; 2198248; 9097040; 9278503; 1944537; 10871403; 10612397; 10360178 [H]