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The map label for this gene is ecoRIIM [H]

Identifier: 186476891

GI number: 186476891

Start: 2402371

End: 2403657

Strand: Direct

Name: ecoRIIM [H]

Synonym: Bphy_2140

Alternate gene names: 186476891

Gene position: 2402371-2403657 (Clockwise)

Preceding gene: 186476890

Following gene: 186476892

Centisome position: 69.05

GC content: 64.88

Gene sequence:

>1287_bases
GTGACCCAGGCTTCACCGCTTGAACTGCTGAAGCAGGCGCGCACCCGCTTCACACAAAAAGAAATCGCCGCGCATGTCGG
CAAGGACATCAAGACGGTGCGCCGCTGGGAAAAGGGCGAAACGCCGTGTCCCGCGATGCTCGAGCCAGCGCTGCACGCGC
TACTGCACGCAGCGCCGGCGCGCAACCGGGACCCAGGCGACGGATCGCGCGTGCACTTCATCGACCTGTTCGCCGGCATC
GGCGGCATCCGCATGGGCTTCGAGGCACACGGCGGCCAATGCGTGTTCACCAGCGAGTGGAACGTCTTCTCCCAGAAAAC
CTACCGGGAAAACTTCGGCGAACACTGCGGCGATGCCGCGCCCCAGCACACGCTCATCGGCGACATCGTCACGTTTCCTG
CCGAAGCCGTTCCCGAGCACGACATCCTGCTCGGCGGCTTTCCGTGCCAGCCGTTTTCGATCGCGGGCGTCAGCAAGAAA
AACGCGCTCGGCCGGCCGCACGGGTTCGAATGCGCGACGCAAGGCACGCTGTTCTTCGATGTCGCGCGCATCATCGCCGC
GCGCCGTCCTGCTGCATTTCTGCTCGAAAACGTGAAGAACCTGCTGTCACACGACAAGGGCCGCACCTTCGATGTGATCC
TGCAGACGCTTCGCGACGAACTGGGCTACGAGGTGCACTACCGGGTGATCGACGGCGCGCATTTCACGCCGCAGCATCGC
GAACGGATCATCATGATCGGCTTTCGCGCACGAACCTCCTTTTCGTGGGACGACCTGCGCCTGCCCGAAGAAGGCCCTCG
GCTCGCGTCGATCCTGCATCGCACGGACGGCACGGAACCCGTGCTGCCGTGGGACGGCGACCGCTTCTTCGATCACGCTG
CGCGCCATGTGCAACCGAAATACACGCTGACGCCTAAGCTCTGGACCTATCTGCAGAATTACGCGGACAAGCATCGCGCG
GCGGGCAACGGATTCGGCTATGGCATGGCGTATCCGCAAAGCGTGACGCGCACGCTGTCGGCCCGCTATCACAAGGACGG
CAGCGAAATTCTGGTCTACCAAGGGGACAAGCAGCGCCCGCGCCGCCTGACGCCGCGCGAGTGCGCACGGCTGATGGGCT
TTCCCGACACCTATCGCATCCCCGTCAGCGACACGCAGGCGTACCGCCAGTTTGGCAACAGCGTGGTGGTGCCCGTCATG
CGCGAGGTCGCGCGCATCATGCTGCCGCATGTGCTCGCGCTGACGCGCGCGGCGCACGACGCCAGCGCTGCAGAACTGGC
GGCCTGA

Upstream 100 bases:

>100_bases
TCTCATTCCCACGGGCAATTCGCCCCCAAACAGCACACGCTCTCGAATTGGGGACATTCATAGGTCATAATGTCCGCAAA
AATACCCAGCAAGGACTCCT

Downstream 100 bases:

>100_bases
TGGTCGACATTGTCGACGCGGTCACGCGCAGCCGGATGATGTCCGGCATTCGCGGCCGCAACACCAAGCCCGAAATCCTG
ATCCGCAGCCTGCTTCACCG

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.EcoRII; Cytosine-specific methyltransferase EcoRII [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTQASPLELLKQARTRFTQKEIAAHVGKDIKTVRRWEKGETPCPAMLEPALHALLHAAPARNRDPGDGSRVHFIDLFAGI
GGIRMGFEAHGGQCVFTSEWNVFSQKTYRENFGEHCGDAAPQHTLIGDIVTFPAEAVPEHDILLGGFPCQPFSIAGVSKK
NALGRPHGFECATQGTLFFDVARIIAARRPAAFLLENVKNLLSHDKGRTFDVILQTLRDELGYEVHYRVIDGAHFTPQHR
ERIIMIGFRARTSFSWDDLRLPEEGPRLASILHRTDGTEPVLPWDGDRFFDHAARHVQPKYTLTPKLWTYLQNYADKHRA
AGNGFGYGMAYPQSVTRTLSARYHKDGSEILVYQGDKQRPRRLTPRECARLMGFPDTYRIPVSDTQAYRQFGNSVVVPVM
REVARIMLPHVLALTRAAHDASAAELAA

Sequences:

>Translated_428_residues
MTQASPLELLKQARTRFTQKEIAAHVGKDIKTVRRWEKGETPCPAMLEPALHALLHAAPARNRDPGDGSRVHFIDLFAGI
GGIRMGFEAHGGQCVFTSEWNVFSQKTYRENFGEHCGDAAPQHTLIGDIVTFPAEAVPEHDILLGGFPCQPFSIAGVSKK
NALGRPHGFECATQGTLFFDVARIIAARRPAAFLLENVKNLLSHDKGRTFDVILQTLRDELGYEVHYRVIDGAHFTPQHR
ERIIMIGFRARTSFSWDDLRLPEEGPRLASILHRTDGTEPVLPWDGDRFFDHAARHVQPKYTLTPKLWTYLQNYADKHRA
AGNGFGYGMAYPQSVTRTLSARYHKDGSEILVYQGDKQRPRRLTPRECARLMGFPDTYRIPVSDTQAYRQFGNSVVVPVM
REVARIMLPHVLALTRAAHDASAAELAA
>Mature_427_residues
TQASPLELLKQARTRFTQKEIAAHVGKDIKTVRRWEKGETPCPAMLEPALHALLHAAPARNRDPGDGSRVHFIDLFAGIG
GIRMGFEAHGGQCVFTSEWNVFSQKTYRENFGEHCGDAAPQHTLIGDIVTFPAEAVPEHDILLGGFPCQPFSIAGVSKKN
ALGRPHGFECATQGTLFFDVARIIAARRPAAFLLENVKNLLSHDKGRTFDVILQTLRDELGYEVHYRVIDGAHFTPQHRE
RIIMIGFRARTSFSWDDLRLPEEGPRLASILHRTDGTEPVLPWDGDRFFDHAARHVQPKYTLTPKLWTYLQNYADKHRAA
GNGFGYGMAYPQSVTRTLSARYHKDGSEILVYQGDKQRPRRLTPRECARLMGFPDTYRIPVSDTQAYRQFGNSVVVPVMR
EVARIMLPHVLALTRAAHDASAAELAA

Specific function: This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=391, Percent_Identity=54.2199488491049, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 47997; Mature: 47866

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQASPLELLKQARTRFTQKEIAAHVGKDIKTVRRWEKGETPCPAMLEPALHALLHAAPA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
RNRDPGDGSRVHFIDLFAGIGGIRMGFEAHGGQCVFTSEWNVFSQKTYRENFGEHCGDAA
CCCCCCCCCEEEEEEHHHCCCHHEECEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCC
PQHTLIGDIVTFPAEAVPEHDILLGGFPCQPFSIAGVSKKNALGRPHGFECATQGTLFFD
CCHHHHHHHHHCCHHHCCCCCEEECCCCCCCCEECCCCCCCCCCCCCCCCHHCCCHHHHH
VARIIAARRPAAFLLENVKNLLSHDKGRTFDVILQTLRDELGYEVHYRVIDGAHFTPQHR
HHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHC
ERIIMIGFRARTSFSWDDLRLPEEGPRLASILHRTDGTEPVLPWDGDRFFDHAARHVQPK
CEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCC
YTLTPKLWTYLQNYADKHRAAGNGFGYGMAYPQSVTRTLSARYHKDGSEILVYQGDKQRP
EEECHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCC
RRLTPRECARLMGFPDTYRIPVSDTQAYRQFGNSVVVPVMREVARIMLPHVLALTRAAHD
CCCCHHHHHHHHCCCCCEECCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCC
ASAAELAA
CCHHHHCC
>Mature Secondary Structure 
TQASPLELLKQARTRFTQKEIAAHVGKDIKTVRRWEKGETPCPAMLEPALHALLHAAPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
RNRDPGDGSRVHFIDLFAGIGGIRMGFEAHGGQCVFTSEWNVFSQKTYRENFGEHCGDAA
CCCCCCCCCEEEEEEHHHCCCHHEECEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCC
PQHTLIGDIVTFPAEAVPEHDILLGGFPCQPFSIAGVSKKNALGRPHGFECATQGTLFFD
CCHHHHHHHHHCCHHHCCCCCEEECCCCCCCCEECCCCCCCCCCCCCCCCHHCCCHHHHH
VARIIAARRPAAFLLENVKNLLSHDKGRTFDVILQTLRDELGYEVHYRVIDGAHFTPQHR
HHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHC
ERIIMIGFRARTSFSWDDLRLPEEGPRLASILHRTDGTEPVLPWDGDRFFDHAARHVQPK
CEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCC
YTLTPKLWTYLQNYADKHRAAGNGFGYGMAYPQSVTRTLSARYHKDGSEILVYQGDKQRP
EEECHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCC
RRLTPRECARLMGFPDTYRIPVSDTQAYRQFGNSVVVPVMREVARIMLPHVLALTRAAHD
CCCCHHHHHHHHCCCCCEECCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCC
ASAAELAA
CCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029675 [H]