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The map label for this gene is 186476848

Identifier: 186476848

GI number: 186476848

Start: 2352877

End: 2354157

Strand: Direct

Name: 186476848

Synonym: Bphy_2094

Alternate gene names: NA

Gene position: 2352877-2354157 (Clockwise)

Preceding gene: 186476847

Following gene: 186476850

Centisome position: 67.63

GC content: 61.12

Gene sequence:

>1281_bases
ATGCTCTATCAATTCCACGAATTCCAGCGGGCGCTTTTGAGCCCGCTCACCGCCTGGGCTCAGGCCGCCTCGAAATCGTT
TGCGAATCCGGCCAGTCCGTTCGCCTATGTGCCAGGCGCGACACGCCTCTCCGCCGGCTACGAACTGCTCTACCGTCTCG
GCAAGGACTACGAGAAACCCGAGTTCAACCTGCATCAGATCGTCAAGGACGGCCGCAACATCCCCATCGTCGAGCAGACG
ATTGTCGAAAAACCGTTCTGCCGCCTGCTGCGCTTCAAGCGCTATTCGGACGACAGCGACGCTGTCACCCAACTCAAGGA
AGAGCCCGTGGTGCTGGTCTGCGCGCCGCTGTCGGGCCACCATTCCACGCTGCTGCGCGACACCGTGCGCACGCTGCTGC
AGGATCACAAGGTGTACATCACCGACTGGATCGACGCCCGTATGGTGCCGATCGAAGACGGCCCATTCGACCTCGACGAT
TACATCGCTTACATCCAGGAATTCATCCGCCACATCGGTGCGAAGAATCTGCACGTGATCTCGGTCTGCCAGCCGACGGT
GCCCGTGCTCGCCGCCATTTCGCTGATGGCGAGCCGCGGTGAAGACACGCCGCGCACCATGACGATGATGGGCGGCCCGA
TCGACGCGCGTAAGAGCCCGACGGCCGTCAATTCGCTGGCGACGCAGCACTCGATCGAATGGTTCGAGAACAACGTGATC
TACAACGTGCCGTCGAACTATCCGGGCTTCGGCCGCAAGGTGTATCCGGGCTTTTTGCAGCACACGGGCTTTGTCGCGAT
GAATCCGGAGCGTCATGCAGCGTCGCACTGGGACTTCTACCAGAGCATGCTGCGCGGCGACGAAGAAGATGCGGAAGCAC
ATCGCCGCTTCTACGACGAATACAACGCGGTGCTCGACATGGCTGCCGAGTACTATCTGCAGACCATCCGCATCGTGTTC
CAGGAATTCAGCCTCGCCGAAGGCACGTGGGAAGTGAACGGCCAGCCCGTGCGCCCGCAGGACATCAGGAAGACCGCGCT
CTTCACGATCGAAGGCGAACTCGACGACATTTCCGGCAGCGGCCAGACGCGCGCCGCCCATGAGCTGTGCACGGGCATTC
CAGAGCAAGATCGTCGTCACTTCACTGCCGAGAAGTGCGGGCACTACGGTATTTTCTCGGGCCGCCGCTGGCGCACAATC
ATCTATCCGCAACTGCGCGACTTCATTCTCGAGCACACGCAGAAGGCAACGCGCAAGGCGGAAGAACGCGTCGAAGCGTA
A

Upstream 100 bases:

>100_bases
ATCCATCACCAACCATCCACACGTAACCAGAAAACGGCCCGCCTCGTCGCTGCGCGTCAAACACGCTGCCCCTGCGCGAA
CCGCTAACGCTGGAGCACCC

Downstream 100 bases:

>100_bases
GGGGCGGGATCGGCACGCAACGCGTGTTCGGCAGGCGACGCGAGAGGTTGCGCCTGCATCCGCCCGACATGAAGACTGAT
GAATGCAAAAAACGGCCTCC

Product: polyhydroxyalkanoate depolymerase, intracellular

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; PHB De-Polymerase; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLYQFHEFQRALLSPLTAWAQAASKSFANPASPFAYVPGATRLSAGYELLYRLGKDYEKPEFNLHQIVKDGRNIPIVEQT
IVEKPFCRLLRFKRYSDDSDAVTQLKEEPVVLVCAPLSGHHSTLLRDTVRTLLQDHKVYITDWIDARMVPIEDGPFDLDD
YIAYIQEFIRHIGAKNLHVISVCQPTVPVLAAISLMASRGEDTPRTMTMMGGPIDARKSPTAVNSLATQHSIEWFENNVI
YNVPSNYPGFGRKVYPGFLQHTGFVAMNPERHAASHWDFYQSMLRGDEEDAEAHRRFYDEYNAVLDMAAEYYLQTIRIVF
QEFSLAEGTWEVNGQPVRPQDIRKTALFTIEGELDDISGSGQTRAAHELCTGIPEQDRRHFTAEKCGHYGIFSGRRWRTI
IYPQLRDFILEHTQKATRKAEERVEA

Sequences:

>Translated_426_residues
MLYQFHEFQRALLSPLTAWAQAASKSFANPASPFAYVPGATRLSAGYELLYRLGKDYEKPEFNLHQIVKDGRNIPIVEQT
IVEKPFCRLLRFKRYSDDSDAVTQLKEEPVVLVCAPLSGHHSTLLRDTVRTLLQDHKVYITDWIDARMVPIEDGPFDLDD
YIAYIQEFIRHIGAKNLHVISVCQPTVPVLAAISLMASRGEDTPRTMTMMGGPIDARKSPTAVNSLATQHSIEWFENNVI
YNVPSNYPGFGRKVYPGFLQHTGFVAMNPERHAASHWDFYQSMLRGDEEDAEAHRRFYDEYNAVLDMAAEYYLQTIRIVF
QEFSLAEGTWEVNGQPVRPQDIRKTALFTIEGELDDISGSGQTRAAHELCTGIPEQDRRHFTAEKCGHYGIFSGRRWRTI
IYPQLRDFILEHTQKATRKAEERVEA
>Mature_426_residues
MLYQFHEFQRALLSPLTAWAQAASKSFANPASPFAYVPGATRLSAGYELLYRLGKDYEKPEFNLHQIVKDGRNIPIVEQT
IVEKPFCRLLRFKRYSDDSDAVTQLKEEPVVLVCAPLSGHHSTLLRDTVRTLLQDHKVYITDWIDARMVPIEDGPFDLDD
YIAYIQEFIRHIGAKNLHVISVCQPTVPVLAAISLMASRGEDTPRTMTMMGGPIDARKSPTAVNSLATQHSIEWFENNVI
YNVPSNYPGFGRKVYPGFLQHTGFVAMNPERHAASHWDFYQSMLRGDEEDAEAHRRFYDEYNAVLDMAAEYYLQTIRIVF
QEFSLAEGTWEVNGQPVRPQDIRKTALFTIEGELDDISGSGQTRAAHELCTGIPEQDRRHFTAEKCGHYGIFSGRRWRTI
IYPQLRDFILEHTQKATRKAEERVEA

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.1.-

Molecular weight: Translated: 48722; Mature: 48722

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYQFHEFQRALLSPLTAWAQAASKSFANPASPFAYVPGATRLSAGYELLYRLGKDYEKP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCC
EFNLHQIVKDGRNIPIVEQTIVEKPFCRLLRFKRYSDDSDAVTQLKEEPVVLVCAPLSGH
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCC
HSTLLRDTVRTLLQDHKVYITDWIDARMVPIEDGPFDLDDYIAYIQEFIRHIGAKNLHVI
HHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
SVCQPTVPVLAAISLMASRGEDTPRTMTMMGGPIDARKSPTAVNSLATQHSIEWFENNVI
EECCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEE
YNVPSNYPGFGRKVYPGFLQHTGFVAMNPERHAASHWDFYQSMLRGDEEDAEAHRRFYDE
EECCCCCCCCCCCCCCHHHHHCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YNAVLDMAAEYYLQTIRIVFQEFSLAEGTWEVNGQPVRPQDIRKTALFTIEGELDDISGS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHEEEEEEECCCCCCCCC
GQTRAAHELCTGIPEQDRRHFTAEKCGHYGIFSGRRWRTIIYPQLRDFILEHTQKATRKA
CCHHHHHHHHCCCCCHHHHHCCHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHH
EERVEA
HHHCCC
>Mature Secondary Structure
MLYQFHEFQRALLSPLTAWAQAASKSFANPASPFAYVPGATRLSAGYELLYRLGKDYEKP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCC
EFNLHQIVKDGRNIPIVEQTIVEKPFCRLLRFKRYSDDSDAVTQLKEEPVVLVCAPLSGH
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCC
HSTLLRDTVRTLLQDHKVYITDWIDARMVPIEDGPFDLDDYIAYIQEFIRHIGAKNLHVI
HHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
SVCQPTVPVLAAISLMASRGEDTPRTMTMMGGPIDARKSPTAVNSLATQHSIEWFENNVI
EECCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEE
YNVPSNYPGFGRKVYPGFLQHTGFVAMNPERHAASHWDFYQSMLRGDEEDAEAHRRFYDE
EECCCCCCCCCCCCCCHHHHHCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YNAVLDMAAEYYLQTIRIVFQEFSLAEGTWEVNGQPVRPQDIRKTALFTIEGELDDISGS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHEEEEEEECCCCCCCCC
GQTRAAHELCTGIPEQDRRHFTAEKCGHYGIFSGRRWRTIIYPQLRDFILEHTQKATRKA
CCHHHHHHHHCCCCCHHHHHCCHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHH
EERVEA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA