Click here to switch to the map view.

The map label for this gene is rnfB [H]

Identifier: 186476850

GI number: 186476850

Start: 2355312

End: 2356187

Strand: Direct

Name: rnfB [H]

Synonym: Bphy_2096

Alternate gene names: 186476850

Gene position: 2355312-2356187 (Clockwise)

Preceding gene: 186476848

Following gene: 186476851

Centisome position: 67.7

GC content: 66.1

Gene sequence:

>876_bases
GTGACAGAGCCCAAAACATTCGCGGATCTCATCGAAGATCTGCTGCCGCAGACACAATGCACGAAGTGCGGCTACCCTGA
CTGCCGCGCTTACGCCGAGGCCATCGCGCAAGGCGAGGCCAGCTACAACCAGTGCCCGCCGGGCGGAGCCGAGGGCGTCG
CGCGCCTCGCGAAGCTGCTCGGCAAGCCCGTGATACCCCTCAATCCCGAGAACGGCGTCGAGCGCCCGAGGCCCGTCGCG
TTCATCGACGAAAACCTGTGCATCGGCTGCACGCTCTGCATGCAGGCCTGTCCCGTCGACGCCATCGTCGGTGCGCCGAA
ACAGATGCACACGATCGTCGCCGAGCTCTGCACAGGTTGCGACCTGTGCGTGCCGCCCTGCCCCGTCGACTGCATCGCAA
TGATCCCGGTGACGGGCGACAAGACGGGCTGGGACGCGTGGACCCAGTCGCAGGCGAACGCGGCACGCGAGCGTCACGAT
GCTCGCCGCGCGCGTCTTGCAGCGGAACGCGAGGCCGCGGAGGCACGCGCGGCCGCGCGGCGCGCATCGTCGACGGCGGC
TCGAAGTTCAGCGGGTTCGGCAGCGCCGGCCGGTAACGTGGCCCCGGTGGAACCATCGACGCAAGCGGCTGCCGCTCCCG
TCAACGACGCGGAAGCGAAAAAACGCGCGATCATCCAGGCCGCGCTCGAACGCGCGCGCAAGAAAAAGGAAGACAACGCG
GCGCAAGGCCTGGGACCGAAGAACACGACCAACATCAGTGCAGACATGCAGGCACAGATCGATGAAGTCGAAGCGCGCCG
TCGCCGTCTAGGCATTGCCACAGACATTTCCGACTCAACGTCCAAGCAGAACGACAATCAATCCGACAAGCCCTGA

Upstream 100 bases:

>100_bases
CTTCGCACAATGCCAGCAGTGCAGTTGAAACCGCATCCATTGAACCGCCGACATTCGCCGCGGCGATCGACGGCAACAGC
AAGTCCCCCACGTCCCGACC

Downstream 100 bases:

>100_bases
CTGCACCGCATGAACGCGACCAAACGACGTGCGATCTACGAGACGCTCCAAAGCCTGAATCCCCATCCGAAGACGGAACT
GGAATACACAACGCCGTTCG

Product: ferredoxin

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTEPKTFADLIEDLLPQTQCTKCGYPDCRAYAEAIAQGEASYNQCPPGGAEGVARLAKLLGKPVIPLNPENGVERPRPVA
FIDENLCIGCTLCMQACPVDAIVGAPKQMHTIVAELCTGCDLCVPPCPVDCIAMIPVTGDKTGWDAWTQSQANAARERHD
ARRARLAAEREAAEARAAARRASSTAARSSAGSAAPAGNVAPVEPSTQAAAAPVNDAEAKKRAIIQAALERARKKKEDNA
AQGLGPKNTTNISADMQAQIDEVEARRRRLGIATDISDSTSKQNDNQSDKP

Sequences:

>Translated_291_residues
MTEPKTFADLIEDLLPQTQCTKCGYPDCRAYAEAIAQGEASYNQCPPGGAEGVARLAKLLGKPVIPLNPENGVERPRPVA
FIDENLCIGCTLCMQACPVDAIVGAPKQMHTIVAELCTGCDLCVPPCPVDCIAMIPVTGDKTGWDAWTQSQANAARERHD
ARRARLAAEREAAEARAAARRASSTAARSSAGSAAPAGNVAPVEPSTQAAAAPVNDAEAKKRAIIQAALERARKKKEDNA
AQGLGPKNTTNISADMQAQIDEVEARRRRLGIATDISDSTSKQNDNQSDKP
>Mature_290_residues
TEPKTFADLIEDLLPQTQCTKCGYPDCRAYAEAIAQGEASYNQCPPGGAEGVARLAKLLGKPVIPLNPENGVERPRPVAF
IDENLCIGCTLCMQACPVDAIVGAPKQMHTIVAELCTGCDLCVPPCPVDCIAMIPVTGDKTGWDAWTQSQANAARERHDA
RRARLAAEREAAEARAAARRASSTAARSSAGSAAPAGNVAPVEPSTQAAAAPVNDAEAKKRAIIQAALERARKKKEDNAA
QGLGPKNTTNISADMQAQIDEVEARRRRLGIATDISDSTSKQNDNQSDKP

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=152, Percent_Identity=51.3157894736842, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 30742; Mature: 30611

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPKTFADLIEDLLPQTQCTKCGYPDCRAYAEAIAQGEASYNQCPPGGAEGVARLAKLL
CCCCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
GKPVIPLNPENGVERPRPVAFIDENLCIGCTLCMQACPVDAIVGAPKQMHTIVAELCTGC
CCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCC
DLCVPPCPVDCIAMIPVTGDKTGWDAWTQSQANAARERHDARRARLAAEREAAEARAAAR
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RASSTAARSSAGSAAPAGNVAPVEPSTQAAAAPVNDAEAKKRAIIQAALERARKKKEDNA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AQGLGPKNTTNISADMQAQIDEVEARRRRLGIATDISDSTSKQNDNQSDKP
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEPKTFADLIEDLLPQTQCTKCGYPDCRAYAEAIAQGEASYNQCPPGGAEGVARLAKLL
CCCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
GKPVIPLNPENGVERPRPVAFIDENLCIGCTLCMQACPVDAIVGAPKQMHTIVAELCTGC
CCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCC
DLCVPPCPVDCIAMIPVTGDKTGWDAWTQSQANAARERHDARRARLAAEREAAEARAAAR
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RASSTAARSSAGSAAPAGNVAPVEPSTQAAAAPVNDAEAKKRAIIQAALERARKKKEDNA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AQGLGPKNTTNISADMQAQIDEVEARRRRLGIATDISDSTSKQNDNQSDKP
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA