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The map label for this gene is mscS [H]

Identifier: 186475979

GI number: 186475979

Start: 1368828

End: 1369745

Strand: Reverse

Name: mscS [H]

Synonym: Bphy_1220

Alternate gene names: 186475979

Gene position: 1369745-1368828 (Counterclockwise)

Preceding gene: 186475982

Following gene: 186475978

Centisome position: 39.37

GC content: 65.36

Gene sequence:

>918_bases
ATGGCAACCGTTCGGGGGCCGATACCGCCTGCATCGAGACACTTTCAAACCCTGGCTATCATGGATGATCTGATTGCTCG
CCTGTTTCACCTGCCGCCTTCGCTGATCGTGTCGCTCACGCATGACCTGATGCATGCGGGCGTGGCGATCCTGATGCTGA
TCGTCGGTTGGTGGCTGTCCAACCGGATCGGCACGTTGTTGTCGACGGCAATGTCCCGCACGCGCGCGGATCCGACGCTC
GCGCCGATGGTGAATGCAATCGGCGCATGGACCATACGCGTGCTCGTACTCTTCGCTGCCCTCAGCGAGGTCGGCGTCGC
GACCGCGAGCGTGCTGGCCGTGCTCGGTGCAGCCGGCCTCGCAATCGGACTTGCGCTGCAAGGCACGCTGCAGAATATTG
CCGCCGGCATCATGTTGCTGATGCTGCGGCCGTTTCGTGCCGGTGACCTGATAGAAGGTACGGGAGCAACGGCCGGCATC
GTGCGCGAAGTGGGACTCTTTACGACGCGCATCGAACGCAGCGACGGCAATGCCGTGTTCGTTCCGAACAGCCAGATCTG
GTCCAACCCAGTGATCAACTACAGCAGTGGTGGCACGCAGCGCGTCGAAGTGGAAGTGGCGATCGCGCAGCGCAAGGACG
TCGCATGCGCGATCGATGCGCTGAAAAAGATGATCGCCGGCGATCCGCGCGTGCTCGGCGGCACTACGCTCGCGCCCGTG
GTGACGGCGGCCGACTACCGGCGCGGCGGGGGCGCGGCGGTCCGCCTCGCCGTATGGGTGAGCGGAGCGGACGCGCAACT
CGCCGGCGAGGACATGCGCGAGCATGCGCGGTCCGTACTCCAGGAGGCAGGCTGCGAAATGGCGGAAGCGCCTCGCGGGC
AAGCTGTGCAGCGCCCTCCTCAGACGCAGCACGCCTGA

Upstream 100 bases:

>100_bases
GCCAAGTGTAGAGCAAGTGAGTCGCGCCTGCCCGTCTGCATTTCACGACACTCAAGCGCGGCACGTTACACGATGTTACC
AAGCCCGCGCAGCGACTCAC

Downstream 100 bases:

>100_bases
CAAGCGTCCTTCTGCTCTGCGTTGCGATGCACGATCGTGCAATGGACTGAATGCCGGTTCATGCACCGCACTGCAACGCA
CGACAGTTCATCGCCGAGGC

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MATVRGPIPPASRHFQTLAIMDDLIARLFHLPPSLIVSLTHDLMHAGVAILMLIVGWWLSNRIGTLLSTAMSRTRADPTL
APMVNAIGAWTIRVLVLFAALSEVGVATASVLAVLGAAGLAIGLALQGTLQNIAAGIMLLMLRPFRAGDLIEGTGATAGI
VREVGLFTTRIERSDGNAVFVPNSQIWSNPVINYSSGGTQRVEVEVAIAQRKDVACAIDALKKMIAGDPRVLGGTTLAPV
VTAADYRRGGGAAVRLAVWVSGADAQLAGEDMREHARSVLQEAGCEMAEAPRGQAVQRPPQTQHA

Sequences:

>Translated_305_residues
MATVRGPIPPASRHFQTLAIMDDLIARLFHLPPSLIVSLTHDLMHAGVAILMLIVGWWLSNRIGTLLSTAMSRTRADPTL
APMVNAIGAWTIRVLVLFAALSEVGVATASVLAVLGAAGLAIGLALQGTLQNIAAGIMLLMLRPFRAGDLIEGTGATAGI
VREVGLFTTRIERSDGNAVFVPNSQIWSNPVINYSSGGTQRVEVEVAIAQRKDVACAIDALKKMIAGDPRVLGGTTLAPV
VTAADYRRGGGAAVRLAVWVSGADAQLAGEDMREHARSVLQEAGCEMAEAPRGQAVQRPPQTQHA
>Mature_304_residues
ATVRGPIPPASRHFQTLAIMDDLIARLFHLPPSLIVSLTHDLMHAGVAILMLIVGWWLSNRIGTLLSTAMSRTRADPTLA
PMVNAIGAWTIRVLVLFAALSEVGVATASVLAVLGAAGLAIGLALQGTLQNIAAGIMLLMLRPFRAGDLIEGTGATAGIV
REVGLFTTRIERSDGNAVFVPNSQIWSNPVINYSSGGTQRVEVEVAIAQRKDVACAIDALKKMIAGDPRVLGGTTLAPVV
TAADYRRGGGAAVRLAVWVSGADAQLAGEDMREHARSVLQEAGCEMAEAPRGQAVQRPPQTQHA

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=259, Percent_Identity=32.4324324324324, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1787591, Length=158, Percent_Identity=29.1139240506329, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 32042; Mature: 31911

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVRGPIPPASRHFQTLAIMDDLIARLFHLPPSLIVSLTHDLMHAGVAILMLIVGWWLS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRIGTLLSTAMSRTRADPTLAPMVNAIGAWTIRVLVLFAALSEVGVATASVLAVLGAAGL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIGLALQGTLQNIAAGIMLLMLRPFRAGDLIEGTGATAGIVREVGLFTTRIERSDGNAVF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCEEE
VPNSQIWSNPVINYSSGGTQRVEVEVAIAQRKDVACAIDALKKMIAGDPRVLGGTTLAPV
CCCCHHHCCCCEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEECCCHHHHH
VTAADYRRGGGAAVRLAVWVSGADAQLAGEDMREHARSVLQEAGCEMAEAPRGQAVQRPP
HHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCC
QTQHA
CCCCC
>Mature Secondary Structure 
ATVRGPIPPASRHFQTLAIMDDLIARLFHLPPSLIVSLTHDLMHAGVAILMLIVGWWLS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRIGTLLSTAMSRTRADPTLAPMVNAIGAWTIRVLVLFAALSEVGVATASVLAVLGAAGL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIGLALQGTLQNIAAGIMLLMLRPFRAGDLIEGTGATAGIVREVGLFTTRIERSDGNAVF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCEEE
VPNSQIWSNPVINYSSGGTQRVEVEVAIAQRKDVACAIDALKKMIAGDPRVLGGTTLAPV
CCCCHHHCCCCEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEECCCHHHHH
VTAADYRRGGGAAVRLAVWVSGADAQLAGEDMREHARSVLQEAGCEMAEAPRGQAVQRPP
HHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCC
QTQHA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]