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The map label for this gene is metF [H]

Identifier: 183222620

GI number: 183222620

Start: 3387975

End: 3388862

Strand: Direct

Name: metF [H]

Synonym: LEPBI_I3275

Alternate gene names: 183222620

Gene position: 3387975-3388862 (Clockwise)

Preceding gene: 183222619

Following gene: 183222622

Centisome position: 94.12

GC content: 40.43

Gene sequence:

>888_bases
ATGCACATTTCTGAGATCCTTGGTAAAAAACAGACGACGATCAGCTTCGAATTTTTCCCCCCTAAAAATGAGGAAGCTTC
CGAGGATTTGTTTCGAAATATCCAAGAACTTTCCCAAATGAACCCAGCTTACGTAAGTGTCACTTATGGAGCAGGCGGAT
CCACTAGACACTTAACACATGACTTGGTTGTCAAATTACAAAACGAAACGGGATTAACAATTGTTAGTCACCTTACCTGC
GTCGGTTCCACAAAGGAAGAGATTGGTGATATTCTAAAACGTTACGACAAAAGTGGGATTCACAATATTATGGCTCTCCG
AGGTGACCCACCCAAGGGGCAACAAGACTTCCAACAAACAGAGAATGGGTTTGCATATGCCGGCGAGTTGGTAGGTTTTA
TCAAAAAAGAATTTCCTAAAATGGGAATTGGAGTAGCTGGATTTCCAGAAGGACATCCCTCCACACCAAATCGATTGAAA
GAAATTGAATACCTAAAATGGAAAGTAGACCAAGGTGCGGATTATATTTGTACCCAAATGTTTTTTGATAACAACCAATT
TTATGATTTTGTAGAACGTTGTGAAATTGCTGGAATCAAAGTTCCAATCATCGCAGGAATTATGCCAGTCACTTCCAGAA
AGGGAATGGCACGTATGGCTGAACTTTCCTTAGGCACCAATTTCCCTGCAAAATTATTGAAATCTTTATCTCGTGCAGAA
GATGATGTTTATGCTGAGAATGTGGGCATCCACTGGGCTACAGAACAGGTGCGAGATTTATTGGATCACAAAATCGCAGG
CATTCACATGTATACATTAAACAAATCGAAAGCCACTCGTAAAATTTACGAATCACTTGGGGTTCGAAATTTCGATCGTA
TCGTTTGA

Upstream 100 bases:

>100_bases
ATTTTCCCTATGGCTTGCTGTGAAAACAAAAGCTTCAAAAGGTTGGTTACTCGCCAACAAGGGACTTGAGAAAAATATTC
CTGTTTAGAGGATAGGTTTT

Downstream 100 bases:

>100_bases
TTCTTGTTTGGGCTTAAATCCGATGAATGAATTTTGGTGCCAATAACCTTCAAGTACCAAACTTCCCTTAGCATTTGTGA
TTTTTGCCTTTCCTGACAAT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MHISEILGKKQTTISFEFFPPKNEEASEDLFRNIQELSQMNPAYVSVTYGAGGSTRHLTHDLVVKLQNETGLTIVSHLTC
VGSTKEEIGDILKRYDKSGIHNIMALRGDPPKGQQDFQQTENGFAYAGELVGFIKKEFPKMGIGVAGFPEGHPSTPNRLK
EIEYLKWKVDQGADYICTQMFFDNNQFYDFVERCEIAGIKVPIIAGIMPVTSRKGMARMAELSLGTNFPAKLLKSLSRAE
DDVYAENVGIHWATEQVRDLLDHKIAGIHMYTLNKSKATRKIYESLGVRNFDRIV

Sequences:

>Translated_295_residues
MHISEILGKKQTTISFEFFPPKNEEASEDLFRNIQELSQMNPAYVSVTYGAGGSTRHLTHDLVVKLQNETGLTIVSHLTC
VGSTKEEIGDILKRYDKSGIHNIMALRGDPPKGQQDFQQTENGFAYAGELVGFIKKEFPKMGIGVAGFPEGHPSTPNRLK
EIEYLKWKVDQGADYICTQMFFDNNQFYDFVERCEIAGIKVPIIAGIMPVTSRKGMARMAELSLGTNFPAKLLKSLSRAE
DDVYAENVGIHWATEQVRDLLDHKIAGIHMYTLNKSKATRKIYESLGVRNFDRIV
>Mature_295_residues
MHISEILGKKQTTISFEFFPPKNEEASEDLFRNIQELSQMNPAYVSVTYGAGGSTRHLTHDLVVKLQNETGLTIVSHLTC
VGSTKEEIGDILKRYDKSGIHNIMALRGDPPKGQQDFQQTENGFAYAGELVGFIKKEFPKMGIGVAGFPEGHPSTPNRLK
EIEYLKWKVDQGADYICTQMFFDNNQFYDFVERCEIAGIKVPIIAGIMPVTSRKGMARMAELSLGTNFPAKLLKSLSRAE
DDVYAENVGIHWATEQVRDLLDHKIAGIHMYTLNKSKATRKIYESLGVRNFDRIV

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=286, Percent_Identity=34.6153846153846, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1790377, Length=288, Percent_Identity=36.8055555555556, Blast_Score=186, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI25147436, Length=305, Percent_Identity=31.4754098360656, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI25147441, Length=305, Percent_Identity=31.4754098360656, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321313, Length=302, Percent_Identity=35.0993377483444, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6325234, Length=284, Percent_Identity=36.2676056338028, Blast_Score=172, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24662658, Length=242, Percent_Identity=25.2066115702479, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33153; Mature: 33153

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHISEILGKKQTTISFEFFPPKNEEASEDLFRNIQELSQMNPAYVSVTYGAGGSTRHLTH
CCHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHH
DLVVKLQNETGLTIVSHLTCVGSTKEEIGDILKRYDKSGIHNIMALRGDPPKGQQDFQQT
HHHEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEEEECCCCCCCHHHHHHH
ENGFAYAGELVGFIKKEFPKMGIGVAGFPEGHPSTPNRLKEIEYLKWKVDQGADYICTQM
HCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCEEHHH
FFDNNQFYDFVERCEIAGIKVPIIAGIMPVTSRKGMARMAELSLGTNFPAKLLKSLSRAE
HCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCC
DDVYAENVGIHWATEQVRDLLDHKIAGIHMYTLNKSKATRKIYESLGVRNFDRIV
HHHHHHCCCCEEHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure
MHISEILGKKQTTISFEFFPPKNEEASEDLFRNIQELSQMNPAYVSVTYGAGGSTRHLTH
CCHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHH
DLVVKLQNETGLTIVSHLTCVGSTKEEIGDILKRYDKSGIHNIMALRGDPPKGQQDFQQT
HHHEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEEEECCCCCCCHHHHHHH
ENGFAYAGELVGFIKKEFPKMGIGVAGFPEGHPSTPNRLKEIEYLKWKVDQGADYICTQM
HCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCEEHHH
FFDNNQFYDFVERCEIAGIKVPIIAGIMPVTSRKGMARMAELSLGTNFPAKLLKSLSRAE
HCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCC
DDVYAENVGIHWATEQVRDLLDHKIAGIHMYTLNKSKATRKIYESLGVRNFDRIV
HHHHHHCCCCEEHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]