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The map label for this gene is recQ2 [H]

Identifier: 183221869

GI number: 183221869

Start: 2587224

End: 2588669

Strand: Reverse

Name: recQ2 [H]

Synonym: LEPBI_I2503

Alternate gene names: 183221869

Gene position: 2588669-2587224 (Counterclockwise)

Preceding gene: 183221870

Following gene: 183221867

Centisome position: 71.91

GC content: 37.76

Gene sequence:

>1446_bases
ATGAAATTGAATTTACCACTGCAAACCTTTGGCATTTCCGAGTTTCGAGGGAACCAAAAATCTGTCATTGAACATGTATT
AAATGGTGGTAATGCGCTCGTACTCATGCCAACAGGTATGGGGAAGTCTGTTTGTTACCAAATCCCGGCCTTGGAAATGC
CAGGGACATGTATCGTTATATCTCCTCTCATTGCACTAATGAAAGACCAAGTGGATTCTTTGCAAAAAAAAGGGATCAAT
GCCACCTATATCAATTCGAGTTTGAGTCGTTCCGAAAGAATGGAGCGTTATGAAAGATTAAACTCTGGTGGATGGAAAAT
TGTATATGTTTCTCCCGAACGGTTTCAAAAAAAAGAATTTTTGGAAGTGATCCAATCCATTCCAATCTCTTTACTTGCCA
TAGACGAAGCACATTGTATCAGTCAATGGGGGCATGATTTTCGCCCCGATTATACCAAAATTAGGTGGTTCCGTGAAATT
TTAAAATTTCCACCCACCATTGCGCTAACGGCAACCGCTTCCCAACGCGTGCAAAATGATATTCTGGAACAAATGGGTAT
TTTGCCGAAAGACATTCAAATTTTTGATGATGGATTGTTTCGTCCCAATTTACATTTATCAGTTACGGAATGTTTTGATA
CGGATACCAAATACAAATTGATATTAGAAGAATTAAAGTCGAACCAAGAGGTTTCGATTTTATACTTTAGTTTGATCCAA
GATTTAGAAAAAATGAGTCATTGGTTGGATACCAAACGAATACGGCATTTGGTTTACCACGGAAAACGATCCAATCAAGA
TAGAAGTGCCACACTCTCTAAGTTCCTTACATCTGATCAAGTGATCCTACTTGCAACGAATGCATTTGGAATGGGTGTCG
ATAAAAAAAATGTCCGCAATGTTTACCATGTACAAATTCCAGGTAGTATCGAAGCCTATTACCAAGAAATAGGTAGAGCA
GGTCGGGACGGAAAACCATCTCAATGTAAACTATTTTATACGGAAGATGATTTAGCGATTCAAATGGATTTTATTGATTG
GCAAAATCCAGATCTTGGATATTTAAAAAAATTATACCAATTGATGGTGCAAAAAGAAAACCAACTGTCTGCATTGGATT
ACCAAACCATCCAGTCTTTTATGACTTATAAAAATAAATCAGACCACCGCGTACAGACAGCAATCAATTTGTTATGTCGT
GTGGGACTCGTTTCTGGTGATTTAGAGAAAGGTACCTTACAAATAGAAAATCCTTGGGATCCATCCCTCTTTGTACAAGA
AGACCTGGAAATCAAAAAAAAAGAAGGTGGAAAACGCCTCTACCAAATGCTTCAATATACCAAGACGGAAGATTGTAGAA
GAAAGTTTATCCACCAATATTTTGATTCACCATTTACCTCTTGCAACAATTGTGATCGATGTCTCAATGCATCGATCTCA
ACTTAG

Upstream 100 bases:

>100_bases
TTTTACAATTGGTTTGAGAAGTGATTCTTTTTGGATGAATTCATTTCAAACGAAAAATTGTCTCTAAATTATCCAGTATG
TTGCAGAGTGGCGAAAGCTC

Downstream 100 bases:

>100_bases
GCTGCTTTGGATTGATGAAATACTGTATGGTTCGGTTTATAGTAGTACCTTGCTTTTTCTCGAGAAAGGATTTGGATCGA
TGTGGCTACATAATTTTCTT

Product: ATP-dependent DNA helicase RecQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MKLNLPLQTFGISEFRGNQKSVIEHVLNGGNALVLMPTGMGKSVCYQIPALEMPGTCIVISPLIALMKDQVDSLQKKGIN
ATYINSSLSRSERMERYERLNSGGWKIVYVSPERFQKKEFLEVIQSIPISLLAIDEAHCISQWGHDFRPDYTKIRWFREI
LKFPPTIALTATASQRVQNDILEQMGILPKDIQIFDDGLFRPNLHLSVTECFDTDTKYKLILEELKSNQEVSILYFSLIQ
DLEKMSHWLDTKRIRHLVYHGKRSNQDRSATLSKFLTSDQVILLATNAFGMGVDKKNVRNVYHVQIPGSIEAYYQEIGRA
GRDGKPSQCKLFYTEDDLAIQMDFIDWQNPDLGYLKKLYQLMVQKENQLSALDYQTIQSFMTYKNKSDHRVQTAINLLCR
VGLVSGDLEKGTLQIENPWDPSLFVQEDLEIKKKEGGKRLYQMLQYTKTEDCRRKFIHQYFDSPFTSCNNCDRCLNASIS
T

Sequences:

>Translated_481_residues
MKLNLPLQTFGISEFRGNQKSVIEHVLNGGNALVLMPTGMGKSVCYQIPALEMPGTCIVISPLIALMKDQVDSLQKKGIN
ATYINSSLSRSERMERYERLNSGGWKIVYVSPERFQKKEFLEVIQSIPISLLAIDEAHCISQWGHDFRPDYTKIRWFREI
LKFPPTIALTATASQRVQNDILEQMGILPKDIQIFDDGLFRPNLHLSVTECFDTDTKYKLILEELKSNQEVSILYFSLIQ
DLEKMSHWLDTKRIRHLVYHGKRSNQDRSATLSKFLTSDQVILLATNAFGMGVDKKNVRNVYHVQIPGSIEAYYQEIGRA
GRDGKPSQCKLFYTEDDLAIQMDFIDWQNPDLGYLKKLYQLMVQKENQLSALDYQTIQSFMTYKNKSDHRVQTAINLLCR
VGLVSGDLEKGTLQIENPWDPSLFVQEDLEIKKKEGGKRLYQMLQYTKTEDCRRKFIHQYFDSPFTSCNNCDRCLNASIS
T
>Mature_481_residues
MKLNLPLQTFGISEFRGNQKSVIEHVLNGGNALVLMPTGMGKSVCYQIPALEMPGTCIVISPLIALMKDQVDSLQKKGIN
ATYINSSLSRSERMERYERLNSGGWKIVYVSPERFQKKEFLEVIQSIPISLLAIDEAHCISQWGHDFRPDYTKIRWFREI
LKFPPTIALTATASQRVQNDILEQMGILPKDIQIFDDGLFRPNLHLSVTECFDTDTKYKLILEELKSNQEVSILYFSLIQ
DLEKMSHWLDTKRIRHLVYHGKRSNQDRSATLSKFLTSDQVILLATNAFGMGVDKKNVRNVYHVQIPGSIEAYYQEIGRA
GRDGKPSQCKLFYTEDDLAIQMDFIDWQNPDLGYLKKLYQLMVQKENQLSALDYQTIQSFMTYKNKSDHRVQTAINLLCR
VGLVSGDLEKGTLQIENPWDPSLFVQEDLEIKKKEGGKRLYQMLQYTKTEDCRRKFIHQYFDSPFTSCNNCDRCLNASIS
T

Specific function: Probable DNA helicase. Required for DNA repair and intramolecular recombination; probably has overlapping function with recS (AC P50729). It probably acts to help generate ss-DNA from ds-DNA breaks [H]

COG id: COG0514

COG function: function code L; Superfamily II DNA helicase

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Localized throughout the nucleoid in the presence or absence of DNA double- strand breaks [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HRDC domain [H]

Homologues:

Organism=Homo sapiens, GI51242943, Length=398, Percent_Identity=36.1809045226131, Blast_Score=226, Evalue=5e-59,
Organism=Homo sapiens, GI51242947, Length=398, Percent_Identity=36.1809045226131, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI148612816, Length=443, Percent_Identity=34.9887133182844, Blast_Score=225, Evalue=9e-59,
Organism=Homo sapiens, GI4557365, Length=342, Percent_Identity=39.766081871345, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI284005309, Length=377, Percent_Identity=31.5649867374005, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI14591902, Length=342, Percent_Identity=35.9649122807018, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI14591904, Length=342, Percent_Identity=35.9649122807018, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI110735439, Length=344, Percent_Identity=35.4651162790698, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI169218196, Length=344, Percent_Identity=35.4651162790698, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI222352149, Length=344, Percent_Identity=24.1279069767442, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI48994977, Length=334, Percent_Identity=43.1137724550898, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1787016, Length=325, Percent_Identity=22.7692307692308, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788930, Length=313, Percent_Identity=22.0447284345048, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32564293, Length=347, Percent_Identity=39.193083573487, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI71987997, Length=352, Percent_Identity=36.3636363636364, Blast_Score=219, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI71987993, Length=352, Percent_Identity=36.3636363636364, Blast_Score=219, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI25145561, Length=383, Percent_Identity=34.9869451697128, Blast_Score=216, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17552866, Length=326, Percent_Identity=35.5828220858896, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI32565321, Length=376, Percent_Identity=23.6702127659574, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323844, Length=378, Percent_Identity=35.978835978836, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321360, Length=337, Percent_Identity=23.7388724035608, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324217, Length=343, Percent_Identity=23.9067055393586, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646066, Length=344, Percent_Identity=39.8255813953488, Blast_Score=222, Evalue=4e-58,
Organism=Drosophila melanogaster, GI62472181, Length=355, Percent_Identity=36.6197183098592, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24664230, Length=355, Percent_Identity=36.6197183098592, Blast_Score=205, Evalue=6e-53,
Organism=Drosophila melanogaster, GI24664226, Length=355, Percent_Identity=36.6197183098592, Blast_Score=204, Evalue=9e-53,
Organism=Drosophila melanogaster, GI21358123, Length=356, Percent_Identity=33.7078651685393, Blast_Score=181, Evalue=9e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004589
- InterPro:   IPR006293
- InterPro:   IPR002121
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR010997
- InterPro:   IPR018982 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF00570 HRDC; PF09382 RQC [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55408; Mature: 55408

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNLPLQTFGISEFRGNQKSVIEHVLNGGNALVLMPTGMGKSVCYQIPALEMPGTCIVI
CEECCCHHHCCCHHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHHEECCCCCCCCCEEHH
SPLIALMKDQVDSLQKKGINATYINSSLSRSERMERYERLNSGGWKIVYVSPERFQKKEF
HHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHH
LEVIQSIPISLLAIDEAHCISQWGHDFRPDYTKIRWFREILKFPPTIALTATASQRVQND
HHHHHHCCEEEEEECHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHH
ILEQMGILPKDIQIFDDGLFRPNLHLSVTECFDTDTKYKLILEELKSNQEVSILYFSLIQ
HHHHHCCCCCCHHHCCCCCCCCCCCEEEHHHHCCCCHHHHHHHHHHCCCCEEHHHHHHHH
DLEKMSHWLDTKRIRHLVYHGKRSNQDRSATLSKFLTSDQVILLATNAFGMGVDKKNVRN
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
VYHVQIPGSIEAYYQEIGRAGRDGKPSQCKLFYTEDDLAIQMDFIDWQNPDLGYLKKLYQ
EEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHH
LMVQKENQLSALDYQTIQSFMTYKNKSDHRVQTAINLLCRVGLVSGDLEKGTLQIENPWD
HHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
PSLFVQEDLEIKKKEGGKRLYQMLQYTKTEDCRRKFIHQYFDSPFTSCNNCDRCLNASIS
CCEEECCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCC
T
C
>Mature Secondary Structure
MKLNLPLQTFGISEFRGNQKSVIEHVLNGGNALVLMPTGMGKSVCYQIPALEMPGTCIVI
CEECCCHHHCCCHHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHHEECCCCCCCCCEEHH
SPLIALMKDQVDSLQKKGINATYINSSLSRSERMERYERLNSGGWKIVYVSPERFQKKEF
HHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHH
LEVIQSIPISLLAIDEAHCISQWGHDFRPDYTKIRWFREILKFPPTIALTATASQRVQND
HHHHHHCCEEEEEECHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHH
ILEQMGILPKDIQIFDDGLFRPNLHLSVTECFDTDTKYKLILEELKSNQEVSILYFSLIQ
HHHHHCCCCCCHHHCCCCCCCCCCCEEEHHHHCCCCHHHHHHHHHHCCCCEEHHHHHHHH
DLEKMSHWLDTKRIRHLVYHGKRSNQDRSATLSKFLTSDQVILLATNAFGMGVDKKNVRN
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
VYHVQIPGSIEAYYQEIGRAGRDGKPSQCKLFYTEDDLAIQMDFIDWQNPDLGYLKKLYQ
EEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHH
LMVQKENQLSALDYQTIQSFMTYKNKSDHRVQTAINLLCRVGLVSGDLEKGTLQIENPWD
HHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
PSLFVQEDLEIKKKEGGKRLYQMLQYTKTEDCRRKFIHQYFDSPFTSCNNCDRCLNASIS
CCEEECCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9642195 [H]