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The map label for this gene is ykuI [H]

Identifier: 183221304

GI number: 183221304

Start: 2009804

End: 2011042

Strand: Reverse

Name: ykuI [H]

Synonym: LEPBI_I1921

Alternate gene names: 183221304

Gene position: 2011042-2009804 (Counterclockwise)

Preceding gene: 183221305

Following gene: 183221303

Centisome position: 55.87

GC content: 43.26

Gene sequence:

>1239_bases
ATGTTCACAACGGAATCAACGGAAGGAAACCAGTTTTGGAACGAGACATACTTTGTCCCTCATTTCCAACCCATCGTCAA
TGCCATCAATCGCTCCATTTCTGCCTACGAGGTACTCGGCCGGCAGTTCAATCCTGAGGAGAACACCTACCAATCTTTAG
GTGGACTCTTCCACAACCGCGAACACGACCCGGTTCCCATTTATAATATTGACCGGATCTTACGAGAGAAGGCGGTCCAG
ACTCTAAAAGAGAGTTCCCTGCGGACTAAACTATTTTTTAATATGATGCCGAACTTCCTCTCACGAGTCCATCATACCGA
TTTATTTGCCGAAAATTTCCATATCATCCAACTGATCGAAAAGTATGGGATTGATCGCAATCAGGTTGTGATTGAAATCA
CCGAGGATGAATTTGATGGATCCATAGAACGCCTCATCCAAATTGTGCAAATCTTTCGGGATTATGGCTTAAAAATAGCC
ATTGATGATTTGGGGACTGGATTTTCCAATTTGGAACGAATTGGTTACCTCCACCCCGACATCATGAAAGTGGACATTCG
GATCATGCGAGAGAGTTTGAATAAAAACTCTTTCAAACAGGTCCTAGGTGCCATTTCCGAAATGAGCCAAAAATTAGGGA
GCCAACTGCTGTTTGAAGGGATTGAAACGGAGGAAGAGGTGAATTTAGCCCTTTCGATGGGAGCCAATCTCTTACAAGGG
TATTATTTTTCCACACCGAACCCCCATTTTTTAAATCGGAATACGTTTTCCGATAAAATGAAATCCGTCCTCGAAACCTT
TTCCAGCGTTCGTTCTCGGGAGCTCCGAGAAAAGGGAATCCGGGAACAGAAGATCATCGACCAATTGCAAGATTTGTTCT
ATGAGCTTTCGGATTCAAAGGAAGAAGACTTTCCCTTTCGTTTTGGGCAAATCCTTGGTTCTCTCCCACGAGAAATCCTA
AAGGTGTTTGTCTGTGATGCAGAAGGTTACCAAATCACTCCTACGTATGACTTGGATCGGATGAACGGTGGGTATTTGGA
ACGGTCGAGACAAATTGGAAACAATTATGCTTGGAAACCTTATTTTTTAAAACACAAAGAGGAGTCCGAACGTTTCCGTA
AGAAATGGGGTGTGACTTACCCTTTGTATGACATCAATAACCAAAACCAATATGTGATTTTTACCTTTTCTCTGATGGCA
GGGAAGATCCTTGTGGCACAAGTGAGTTGGTCGGAATAG

Upstream 100 bases:

>100_bases
TGTGGATTCTGTTGGTCAAAAATGAGTCCATTTTGTTCCAAAAATGTGGAATTTTTCTTGCCAGTTTGGAACTGAGTTCT
AGGTTGGAAGAAAGGGAACC

Downstream 100 bases:

>100_bases
AATCTTGTTTTTTTTGACTGGTCGTTTTTCCTGGTAGAAGGGATTCGTCTATAATATAGAATCATTCTAAATAAAAAAAC
AGGATACGACCTTGTCCGCA

Product: putative diguanylate phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MFTTESTEGNQFWNETYFVPHFQPIVNAINRSISAYEVLGRQFNPEENTYQSLGGLFHNREHDPVPIYNIDRILREKAVQ
TLKESSLRTKLFFNMMPNFLSRVHHTDLFAENFHIIQLIEKYGIDRNQVVIEITEDEFDGSIERLIQIVQIFRDYGLKIA
IDDLGTGFSNLERIGYLHPDIMKVDIRIMRESLNKNSFKQVLGAISEMSQKLGSQLLFEGIETEEEVNLALSMGANLLQG
YYFSTPNPHFLNRNTFSDKMKSVLETFSSVRSRELREKGIREQKIIDQLQDLFYELSDSKEEDFPFRFGQILGSLPREIL
KVFVCDAEGYQITPTYDLDRMNGGYLERSRQIGNNYAWKPYFLKHKEESERFRKKWGVTYPLYDINNQNQYVIFTFSLMA
GKILVAQVSWSE

Sequences:

>Translated_412_residues
MFTTESTEGNQFWNETYFVPHFQPIVNAINRSISAYEVLGRQFNPEENTYQSLGGLFHNREHDPVPIYNIDRILREKAVQ
TLKESSLRTKLFFNMMPNFLSRVHHTDLFAENFHIIQLIEKYGIDRNQVVIEITEDEFDGSIERLIQIVQIFRDYGLKIA
IDDLGTGFSNLERIGYLHPDIMKVDIRIMRESLNKNSFKQVLGAISEMSQKLGSQLLFEGIETEEEVNLALSMGANLLQG
YYFSTPNPHFLNRNTFSDKMKSVLETFSSVRSRELREKGIREQKIIDQLQDLFYELSDSKEEDFPFRFGQILGSLPREIL
KVFVCDAEGYQITPTYDLDRMNGGYLERSRQIGNNYAWKPYFLKHKEESERFRKKWGVTYPLYDINNQNQYVIFTFSLMA
GKILVAQVSWSE
>Mature_412_residues
MFTTESTEGNQFWNETYFVPHFQPIVNAINRSISAYEVLGRQFNPEENTYQSLGGLFHNREHDPVPIYNIDRILREKAVQ
TLKESSLRTKLFFNMMPNFLSRVHHTDLFAENFHIIQLIEKYGIDRNQVVIEITEDEFDGSIERLIQIVQIFRDYGLKIA
IDDLGTGFSNLERIGYLHPDIMKVDIRIMRESLNKNSFKQVLGAISEMSQKLGSQLLFEGIETEEEVNLALSMGANLLQG
YYFSTPNPHFLNRNTFSDKMKSVLETFSSVRSRELREKGIREQKIIDQLQDLFYELSDSKEEDFPFRFGQILGSLPREIL
KVFVCDAEGYQITPTYDLDRMNGGYLERSRQIGNNYAWKPYFLKHKEESERFRKKWGVTYPLYDINNQNQYVIFTFSLMA
GKILVAQVSWSE

Specific function: Unknown

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1790496, Length=149, Percent_Identity=32.2147651006711, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081921, Length=155, Percent_Identity=30.9677419354839, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082096, Length=154, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788849, Length=132, Percent_Identity=28.7878787878788, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI226510982, Length=252, Percent_Identity=23.8095238095238, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786507, Length=253, Percent_Identity=23.7154150197628, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI87081845, Length=252, Percent_Identity=25, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788502, Length=233, Percent_Identity=25.7510729613734, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787055, Length=233, Percent_Identity=26.6094420600858, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081980, Length=232, Percent_Identity=25.8620689655172, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787541, Length=234, Percent_Identity=22.6495726495727, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87081743, Length=131, Percent_Identity=30.5343511450382, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001633
- InterPro:   IPR018842 [H]

Pfam domain/function: PF00563 EAL; PF10388 YkuI_C [H]

EC number: NA

Molecular weight: Translated: 48249; Mature: 48249

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTTESTEGNQFWNETYFVPHFQPIVNAINRSISAYEVLGRQFNPEENTYQSLGGLFHNR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHCCC
EHDPVPIYNIDRILREKAVQTLKESSLRTKLFFNMMPNFLSRVHHTDLFAENFHIIQLIE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KYGIDRNQVVIEITEDEFDGSIERLIQIVQIFRDYGLKIAIDDLGTGFSNLERIGYLHPD
HHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCCH
IMKVDIRIMRESLNKNSFKQVLGAISEMSQKLGSQLLFEGIETEEEVNLALSMGANLLQG
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHC
YYFSTPNPHFLNRNTFSDKMKSVLETFSSVRSRELREKGIREQKIIDQLQDLFYELSDSK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
EEDFPFRFGQILGSLPREILKVFVCDAEGYQITPTYDLDRMNGGYLERSRQIGNNYAWKP
CCCCCHHHHHHHHHHHHHHHHHHEECCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCCC
YFLKHKEESERFRKKWGVTYPLYDINNQNQYVIFTFSLMAGKILVAQVSWSE
CEECCCHHHHHHHHHCCCCCEEEECCCCCCEEEEEEHHHHCEEEEEEECCCC
>Mature Secondary Structure
MFTTESTEGNQFWNETYFVPHFQPIVNAINRSISAYEVLGRQFNPEENTYQSLGGLFHNR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHCCC
EHDPVPIYNIDRILREKAVQTLKESSLRTKLFFNMMPNFLSRVHHTDLFAENFHIIQLIE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KYGIDRNQVVIEITEDEFDGSIERLIQIVQIFRDYGLKIAIDDLGTGFSNLERIGYLHPD
HHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCCH
IMKVDIRIMRESLNKNSFKQVLGAISEMSQKLGSQLLFEGIETEEEVNLALSMGANLLQG
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHC
YYFSTPNPHFLNRNTFSDKMKSVLETFSSVRSRELREKGIREQKIIDQLQDLFYELSDSK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
EEDFPFRFGQILGSLPREILKVFVCDAEGYQITPTYDLDRMNGGYLERSRQIGNNYAWKP
CCCCCHHHHHHHHHHHHHHHHHHEECCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCCC
YFLKHKEESERFRKKWGVTYPLYDINNQNQYVIFTFSLMAGKILVAQVSWSE
CEECCCHHHHHHHHHCCCCCEEEECCCCCCEEEEEEHHHHCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]