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The map label for this gene is pyrG [H]

Identifier: 183221032

GI number: 183221032

Start: 1719975

End: 1721597

Strand: Direct

Name: pyrG [H]

Synonym: LEPBI_I1645

Alternate gene names: 183221032

Gene position: 1719975-1721597 (Clockwise)

Preceding gene: 183221031

Following gene: 183221033

Centisome position: 47.78

GC content: 39.19

Gene sequence:

>1623_bases
TTGTCCAAGACCAGATATATATTCATTACCGGTGGCGTTTCTTCTTCTTTAGGGAAAGGTGTCACTGTTGCCGCTCTCGG
TTGTTTGCTTGAAGCGAGAGGTTATACCGTCTCTTTACAAAAAATGGATCCTTATATCAACATTGACCCAGGTACTATGA
GCCCGTACCAACATGGCGAAGTGTATGTCACCGAAGATGGTGCCGAAACCGATTTGGATTTAGGATACTATGAACGATTT
ACCAAATCTAAATTTTCACGAAAAAATTCTGTATCCACTGGGCAAATTTACCATGCCGTCATCGAGAGAGAAAGAAAAGG
GGATTATTTAGGTAGAACCGTACAAGTTGTACCACATATCACGAATGAAATTCGAAGCCGTATTTATAACCTAACACGGG
ACCAAGAAACTGACTTTGTCATTGTTGAAATTGGTGGAACTGTGGGAGACATTGAGTCAATTCCATTTTTGGAAGCCATT
AGACAAATGCGTTACGAACATGGTAACCAACAGGTATTATTTCTCCACTTAACGCTTGTCCCAACCATTACTGCAGCTGG
TGAAGCAAAAACAAAACCAACCCAACACTCTGTAAAAGAGTTACTTGCCCTTGGAATCCAACCAGATGTTTTGATCTGCC
GTATCAATAAACCAATGTCCAAGGAGATGAAAAATAAAATCTCTCTTTTTTGTAATGTAAAAGAACAAAATGTAATCTCG
GCGGTTGATATCACTACGTCTATCTATGAAATCCCTCTTATGTATCGGGAAGATAAATTGGATGAAGTGGTTTTGAATGC
TCTTGGAATGGATTTACGAAAACTCAATTTTTCCCAATGGGAAAATATGGTTAAAAAAATCAGGAATACAAAAAAGACCG
TTAAAGTTGCGTTAATCGGGAAATACATTTCACTCCAAGATGCTTATCGTTCCGTATATGAATCTTTGGCACATGGCGGA
ATCGCAAATGATGTCGAAGTGAGTGTCGTCAAAATCAATCCGGAAGATATCGATTCTAAAAATATCAAAGAATTGTTAAA
AGGTGTTCATGGAGTTCTTGTTCCTGGTGGTTTTGGAGAACGTGGCATCGAAGGAAAAATTGCAGCAATTCAATACGCAA
GAACCAAAAACATTCCATTTTTTGGAATTTGTTTAGGAATGCAGTGCGCGGTGATTGAATTTGCAAGGAATGTCCTAGGA
TTCAAAGATGCCAATTCGACTGAATTCAAACCAAATGTGCAATACCCTGTCATTTCGATGATAGAAGAACAAAAGGAAAT
CGAACGAATGGGTGGAACCATGAGACTTGGTGCATATCCTTGTCTTGTTAAAAAAGGAAGCCTTGCCCATTCGGAATACA
AATCGGATCGAATTTCGGAACGTCATCGTCACCGTTTTGAATTTACACTTCGTTATAAGGATGATTTTGAGAAAAAGGGA
ATGAATCTCACTGGGTTTTCGCCTGATGGCAGTTTGGCTGAGATTGTTGAGATTCCAAACCATCCTTGGTTTATCGGTGT
ACAATTCCATCCAGAATTCCAATCGAAACCAACAGACCCACATCCACTCTTTGCAGGTTTTATCAAAGCTGCATCGAAAT
TAGCAAAAAAAACAGAGGATTAG

Upstream 100 bases:

>100_bases
AACCAAGATTTTAGAAAAAGCGAAATCTCCTTCCTAAACGTATTGATTTTGGACCCAAATCCCGACAGTCTGTAAAAAAC
ATTCTTTTTTGAGGTCCAGT

Downstream 100 bases:

>100_bases
TATGTACGATTTAATTGAAGAAAGAGAATTTTTTGGAAAAAAAATCGGAGGTCGTCAGCCGTTTTTTCTTATCTCAGGGC
CTTGTGTGATGGAGAACAAG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSKTRYIFITGGVSSSLGKGVTVAALGCLLEARGYTVSLQKMDPYINIDPGTMSPYQHGEVYVTEDGAETDLDLGYYERF
TKSKFSRKNSVSTGQIYHAVIERERKGDYLGRTVQVVPHITNEIRSRIYNLTRDQETDFVIVEIGGTVGDIESIPFLEAI
RQMRYEHGNQQVLFLHLTLVPTITAAGEAKTKPTQHSVKELLALGIQPDVLICRINKPMSKEMKNKISLFCNVKEQNVIS
AVDITTSIYEIPLMYREDKLDEVVLNALGMDLRKLNFSQWENMVKKIRNTKKTVKVALIGKYISLQDAYRSVYESLAHGG
IANDVEVSVVKINPEDIDSKNIKELLKGVHGVLVPGGFGERGIEGKIAAIQYARTKNIPFFGICLGMQCAVIEFARNVLG
FKDANSTEFKPNVQYPVISMIEEQKEIERMGGTMRLGAYPCLVKKGSLAHSEYKSDRISERHRHRFEFTLRYKDDFEKKG
MNLTGFSPDGSLAEIVEIPNHPWFIGVQFHPEFQSKPTDPHPLFAGFIKAASKLAKKTED

Sequences:

>Translated_540_residues
MSKTRYIFITGGVSSSLGKGVTVAALGCLLEARGYTVSLQKMDPYINIDPGTMSPYQHGEVYVTEDGAETDLDLGYYERF
TKSKFSRKNSVSTGQIYHAVIERERKGDYLGRTVQVVPHITNEIRSRIYNLTRDQETDFVIVEIGGTVGDIESIPFLEAI
RQMRYEHGNQQVLFLHLTLVPTITAAGEAKTKPTQHSVKELLALGIQPDVLICRINKPMSKEMKNKISLFCNVKEQNVIS
AVDITTSIYEIPLMYREDKLDEVVLNALGMDLRKLNFSQWENMVKKIRNTKKTVKVALIGKYISLQDAYRSVYESLAHGG
IANDVEVSVVKINPEDIDSKNIKELLKGVHGVLVPGGFGERGIEGKIAAIQYARTKNIPFFGICLGMQCAVIEFARNVLG
FKDANSTEFKPNVQYPVISMIEEQKEIERMGGTMRLGAYPCLVKKGSLAHSEYKSDRISERHRHRFEFTLRYKDDFEKKG
MNLTGFSPDGSLAEIVEIPNHPWFIGVQFHPEFQSKPTDPHPLFAGFIKAASKLAKKTED
>Mature_539_residues
SKTRYIFITGGVSSSLGKGVTVAALGCLLEARGYTVSLQKMDPYINIDPGTMSPYQHGEVYVTEDGAETDLDLGYYERFT
KSKFSRKNSVSTGQIYHAVIERERKGDYLGRTVQVVPHITNEIRSRIYNLTRDQETDFVIVEIGGTVGDIESIPFLEAIR
QMRYEHGNQQVLFLHLTLVPTITAAGEAKTKPTQHSVKELLALGIQPDVLICRINKPMSKEMKNKISLFCNVKEQNVISA
VDITTSIYEIPLMYREDKLDEVVLNALGMDLRKLNFSQWENMVKKIRNTKKTVKVALIGKYISLQDAYRSVYESLAHGGI
ANDVEVSVVKINPEDIDSKNIKELLKGVHGVLVPGGFGERGIEGKIAAIQYARTKNIPFFGICLGMQCAVIEFARNVLGF
KDANSTEFKPNVQYPVISMIEEQKEIERMGGTMRLGAYPCLVKKGSLAHSEYKSDRISERHRHRFEFTLRYKDDFEKKGM
NLTGFSPDGSLAEIVEIPNHPWFIGVQFHPEFQSKPTDPHPLFAGFIKAASKLAKKTED

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=549, Percent_Identity=44.4444444444444, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI28559085, Length=552, Percent_Identity=43.4782608695652, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI28559083, Length=552, Percent_Identity=43.4782608695652, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI221316689, Length=552, Percent_Identity=43.4782608695652, Blast_Score=470, Evalue=1e-132,
Organism=Escherichia coli, GI1789142, Length=544, Percent_Identity=53.3088235294118, Blast_Score=594, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI25148299, Length=605, Percent_Identity=36.0330578512397, Blast_Score=402, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322563, Length=567, Percent_Identity=41.7989417989418, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6319432, Length=562, Percent_Identity=40.3914590747331, Blast_Score=432, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24664469, Length=549, Percent_Identity=44.2622950819672, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI21357815, Length=494, Percent_Identity=42.914979757085, Blast_Score=393, Evalue=1e-109,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60704; Mature: 60572

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTRYIFITGGVSSSLGKGVTVAALGCLLEARGYTVSLQKMDPYINIDPGTMSPYQHGE
CCCEEEEEEECCCCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCE
VYVTEDGAETDLDLGYYERFTKSKFSRKNSVSTGQIYHAVIERERKGDYLGRTVQVVPHI
EEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHH
TNEIRSRIYNLTRDQETDFVIVEIGGTVGDIESIPFLEAIRQMRYEHGNQQVLFLHLTLV
HHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEH
PTITAAGEAKTKPTQHSVKELLALGIQPDVLICRINKPMSKEMKNKISLFCNVKEQNVIS
HHEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCEEEEEECCCCCCEE
AVDITTSIYEIPLMYREDKLDEVVLNALGMDLRKLNFSQWENMVKKIRNTKKTVKVALIG
EEHHHHHHHHCCEEECCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCHHEEEEEEEE
KYISLQDAYRSVYESLAHGGIANDVEVSVVKINPEDIDSKNIKELLKGVHGVLVPGGFGE
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECHHCCCHHHHHHHHHHHCEEECCCCCCC
RGIEGKIAAIQYARTKNIPFFGICLGMQCAVIEFARNVLGFKDANSTEFKPNVQYPVISM
CCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
IEEQKEIERMGGTMRLGAYPCLVKKGSLAHSEYKSDRISERHRHRFEFTLRYKDDFEKKG
HHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEEECCCHHHCC
MNLTGFSPDGSLAEIVEIPNHPWFIGVQFHPEFQSKPTDPHPLFAGFIKAASKLAKKTED
CEEECCCCCCCHHHHEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKTRYIFITGGVSSSLGKGVTVAALGCLLEARGYTVSLQKMDPYINIDPGTMSPYQHGE
CCEEEEEEECCCCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCE
VYVTEDGAETDLDLGYYERFTKSKFSRKNSVSTGQIYHAVIERERKGDYLGRTVQVVPHI
EEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHH
TNEIRSRIYNLTRDQETDFVIVEIGGTVGDIESIPFLEAIRQMRYEHGNQQVLFLHLTLV
HHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEH
PTITAAGEAKTKPTQHSVKELLALGIQPDVLICRINKPMSKEMKNKISLFCNVKEQNVIS
HHEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCEEEEEECCCCCCEE
AVDITTSIYEIPLMYREDKLDEVVLNALGMDLRKLNFSQWENMVKKIRNTKKTVKVALIG
EEHHHHHHHHCCEEECCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCHHEEEEEEEE
KYISLQDAYRSVYESLAHGGIANDVEVSVVKINPEDIDSKNIKELLKGVHGVLVPGGFGE
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECHHCCCHHHHHHHHHHHCEEECCCCCCC
RGIEGKIAAIQYARTKNIPFFGICLGMQCAVIEFARNVLGFKDANSTEFKPNVQYPVISM
CCCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
IEEQKEIERMGGTMRLGAYPCLVKKGSLAHSEYKSDRISERHRHRFEFTLRYKDDFEKKG
HHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEEECCCHHHCC
MNLTGFSPDGSLAEIVEIPNHPWFIGVQFHPEFQSKPTDPHPLFAGFIKAASKLAKKTED
CEEECCCCCCCHHHHEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA