Click here to switch to the map view.

The map label for this gene is hldE [H]

Identifier: 183221031

GI number: 183221031

Start: 1719423

End: 1719911

Strand: Direct

Name: hldE [H]

Synonym: LEPBI_I1644

Alternate gene names: 183221031

Gene position: 1719423-1719911 (Clockwise)

Preceding gene: 183221030

Following gene: 183221032

Centisome position: 47.77

GC content: 39.06

Gene sequence:

>489_bases
ATGAGTTTTTATCAAGATTTACAAAACAAAATCATTCCATTTGATGCGATAGAATCCAAACGAAAGTCGTTAGAAGGCAA
AAAGATCGTTTTTACAAATGGTTGTTTTGATATTTTACACCCAGGTCATGTTTCATACTTAGCCCAAGCTCGCGATTTAG
GTGACTTACTTTGGATTGGAGTGAACTCTGATGATAGTGTTAGGCGACTGAAAGGAGAATCCAGGCCCATCAATTCTTGC
GAAGACAGGATGTTGGTGCTTGCTGCTTTATCGTCTGTCAATTTTGTATCGAGTTTTTCCGAAGACACACCCCTAACAAT
TCTAAAGAAGGTACAACCATCCATCCATTCCAAGGGTGGGGATTACCAAGTGGAAACCCTCCCAGAATACCAAATTTTAA
AGGAGATGGGTGCGGATATTCAAATTTTACCTTTTGTCTCAGGAAAATCCACAACCAAGATTTTAGAAAAAGCGAAATCT
CCTTCCTAA

Upstream 100 bases:

>100_bases
TCAGTGCCGGGATCGTCGTCGGCAAATTGGGAGCGGCAACGGTCACACAACCCGAAATTGAAGATGCACTTCGAACTCTT
GGTTATTTGGATGAGGCAGT

Downstream 100 bases:

>100_bases
ACGTATTGATTTTGGACCCAAATCCCGACAGTCTGTAAAAAACATTCTTTTTTGAGGTCCAGTTTGTCCAAGACCAGATA
TATATTCATTACCGGTGGCG

Product: nucleotidyltransferase RfaE domain-containing protein

Products: diphosphate; CDPglycerol

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MSFYQDLQNKIIPFDAIESKRKSLEGKKIVFTNGCFDILHPGHVSYLAQARDLGDLLWIGVNSDDSVRRLKGESRPINSC
EDRMLVLAALSSVNFVSSFSEDTPLTILKKVQPSIHSKGGDYQVETLPEYQILKEMGADIQILPFVSGKSTTKILEKAKS
PS

Sequences:

>Translated_162_residues
MSFYQDLQNKIIPFDAIESKRKSLEGKKIVFTNGCFDILHPGHVSYLAQARDLGDLLWIGVNSDDSVRRLKGESRPINSC
EDRMLVLAALSSVNFVSSFSEDTPLTILKKVQPSIHSKGGDYQVETLPEYQILKEMGADIQILPFVSGKSTTKILEKAKS
PS
>Mature_161_residues
SFYQDLQNKIIPFDAIESKRKSLEGKKIVFTNGCFDILHPGHVSYLAQARDLGDLLWIGVNSDDSVRRLKGESRPINSCE
DRMLVLAALSSVNFVSSFSEDTPLTILKKVQPSIHSKGGDYQVETLPEYQILKEMGADIQILPFVSGKSTTKILEKAKSP
S

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=142, Percent_Identity=50, Blast_Score=142, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI193203447, Length=69, Percent_Identity=40.5797101449275, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17509873, Length=69, Percent_Identity=40.5797101449275, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.7.39

Molecular weight: Translated: 18018; Mature: 17887

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFYQDLQNKIIPFDAIESKRKSLEGKKIVFTNGCFDILHPGHVSYLAQARDLGDLLWIG
CCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCHHHCCCCHHHHHHHHHCCCCEEEEE
VNSDDSVRRLKGESRPINSCEDRMLVLAALSSVNFVSSFSEDTPLTILKKVQPSIHSKGG
ECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHCCCCC
DYQVETLPEYQILKEMGADIQILPFVSGKSTTKILEKAKSPS
CEEEEECCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SFYQDLQNKIIPFDAIESKRKSLEGKKIVFTNGCFDILHPGHVSYLAQARDLGDLLWIG
CHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCHHHCCCCHHHHHHHHHCCCCEEEEE
VNSDDSVRRLKGESRPINSCEDRMLVLAALSSVNFVSSFSEDTPLTILKKVQPSIHSKGG
ECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHCCCCC
DYQVETLPEYQILKEMGADIQILPFVSGKSTTKILEKAKSPS
CEEEEECCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; sn-glycerol 3-phosphate

Specific reaction: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA