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The map label for this gene is hldE [H]

Identifier: 183221030

GI number: 183221030

Start: 1718407

End: 1719426

Strand: Direct

Name: hldE [H]

Synonym: LEPBI_I1643

Alternate gene names: 183221030

Gene position: 1718407-1719426 (Clockwise)

Preceding gene: 183221028

Following gene: 183221031

Centisome position: 47.74

GC content: 40.88

Gene sequence:

>1020_bases
TTGTTGAAAATCAAAAAATCTTTGCTACACCAAACTTTTGCCAAACTTTCCCAAATCAAAGTTCTCGTCATTGGCGATTT
GATATTGGATGAATATCTCATTGGTTCGGTCGAAAGAATTTCTCCTGAAGCACCCGTTCCTGTGGTTTGGGTTCGGAACG
AAAAACAAACTTTAGGTGGATCAGGGAACGTCGTACAAAACCTTTCTTCCATTGGTGTGAATGGGATCGTCTATGGTCGA
ATTGGAAACGATAAGGCAGGTGAGAGTTTAGAAAGAATATTACTTTCCAACTCGGTAGCAAAGGAGGATTTGGTTTTACT
CAAATCTAAAACCTTACCAACCATTTTGAAAACTAGAATCATTGCTTCTCACCAACAAGTTTGCCGTGTGGACAGAGAAG
AAATTGTTCCTCTGACGGAAGCAGAAGAGGCAACCATTTTAGAAACTTTAAAACAGAAAATCAAAGAATCTTCTGCGGTG
ATCCTTTCTGATTATGATAAAGGTTATTTAACTCCATCACTCATTCAATCGGTGATCTCACTTTGTAATGCGGAAGAGAA
AATTGTCACTGTAGATCCGCAGGTCAGCCATTTTTTCCTTTATAAAAACATTCATATCATGACACCAAATCATCATGAAG
CAGGAAAAGCTTTAGGAAAAAAATTGGCAAGTGATTCTGAAATTGAATCCGCTTGCCGTGAAATTTCTGAAAGAATCACA
CCCGATGCAATGATGATCACACGAGGGGAGAAAGGGATGTCTATATATGAACGTAAAACCAATACATTTTACCATATCCC
CACAGTGGCAAAGGAAGTGTTTGATGTGACGGGAGCGGGAGACACAGTGATTACCACTTACACAGCGTTTGTTGCCAGTG
GGATGAGTATCAAAGAAGCAGCCCTTGTTTCGAATGTCAGTGCCGGGATCGTCGTCGGCAAATTGGGAGCGGCAACGGTC
ACACAACCCGAAATTGAAGATGCACTTCGAACTCTTGGTTATTTGGATGAGGCAGTATGA

Upstream 100 bases:

>100_bases
GAAACACATCAGGTAAGGGAAAGAGTGGTAAGGGGAAGGTTGACACAGAGAAAGGTTTTAGATTCTATGTTTAGTTGTAA
ACCAAATCAAGGATTCGGCT

Downstream 100 bases:

>100_bases
GTTTTTATCAAGATTTACAAAACAAAATCATTCCATTTGATGCGATAGAATCCAAACGAAAGTCGTTAGAAGGCAAAAAG
ATCGTTTTTACAAATGGTTG

Product: carbohydrate kinase subunit RfaE

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MLKIKKSLLHQTFAKLSQIKVLVIGDLILDEYLIGSVERISPEAPVPVVWVRNEKQTLGGSGNVVQNLSSIGVNGIVYGR
IGNDKAGESLERILLSNSVAKEDLVLLKSKTLPTILKTRIIASHQQVCRVDREEIVPLTEAEEATILETLKQKIKESSAV
ILSDYDKGYLTPSLIQSVISLCNAEEKIVTVDPQVSHFFLYKNIHIMTPNHHEAGKALGKKLASDSEIESACREISERIT
PDAMMITRGEKGMSIYERKTNTFYHIPTVAKEVFDVTGAGDTVITTYTAFVASGMSIKEAALVSNVSAGIVVGKLGAATV
TQPEIEDALRTLGYLDEAV

Sequences:

>Translated_339_residues
MLKIKKSLLHQTFAKLSQIKVLVIGDLILDEYLIGSVERISPEAPVPVVWVRNEKQTLGGSGNVVQNLSSIGVNGIVYGR
IGNDKAGESLERILLSNSVAKEDLVLLKSKTLPTILKTRIIASHQQVCRVDREEIVPLTEAEEATILETLKQKIKESSAV
ILSDYDKGYLTPSLIQSVISLCNAEEKIVTVDPQVSHFFLYKNIHIMTPNHHEAGKALGKKLASDSEIESACREISERIT
PDAMMITRGEKGMSIYERKTNTFYHIPTVAKEVFDVTGAGDTVITTYTAFVASGMSIKEAALVSNVSAGIVVGKLGAATV
TQPEIEDALRTLGYLDEAV
>Mature_339_residues
MLKIKKSLLHQTFAKLSQIKVLVIGDLILDEYLIGSVERISPEAPVPVVWVRNEKQTLGGSGNVVQNLSSIGVNGIVYGR
IGNDKAGESLERILLSNSVAKEDLVLLKSKTLPTILKTRIIASHQQVCRVDREEIVPLTEAEEATILETLKQKIKESSAV
ILSDYDKGYLTPSLIQSVISLCNAEEKIVTVDPQVSHFFLYKNIHIMTPNHHEAGKALGKKLASDSEIESACREISERIT
PDAMMITRGEKGMSIYERKTNTFYHIPTVAKEVFDVTGAGDTVITTYTAFVASGMSIKEAALVSNVSAGIVVGKLGAATV
TQPEIEDALRTLGYLDEAV

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=319, Percent_Identity=37.9310344827586, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 36947; Mature: 36947

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIKKSLLHQTFAKLSQIKVLVIGDLILDEYLIGSVERISPEAPVPVVWVRNEKQTLGG
CCCHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHCCHHHCCCCCCCCEEEECCCHHHCCC
SGNVVQNLSSIGVNGIVYGRIGNDKAGESLERILLSNSVAKEDLVLLKSKTLPTILKTRI
CCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHECCCCCHHHHHHHH
IASHQQVCRVDREEIVPLTEAEEATILETLKQKIKESSAVILSDYDKGYLTPSLIQSVIS
HHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHH
LCNAEEKIVTVDPQVSHFFLYKNIHIMTPNHHEAGKALGKKLASDSEIESACREISERIT
HHCCCCCEEEECCCCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
PDAMMITRGEKGMSIYERKTNTFYHIPTVAKEVFDVTGAGDTVITTYTAFVASGMSIKEA
CCEEEEECCCCCCCHHHHCCCCEEECHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHH
ALVSNVSAGIVVGKLGAATVTQPEIEDALRTLGYLDEAV
HHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKIKKSLLHQTFAKLSQIKVLVIGDLILDEYLIGSVERISPEAPVPVVWVRNEKQTLGG
CCCHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHCCHHHCCCCCCCCEEEECCCHHHCCC
SGNVVQNLSSIGVNGIVYGRIGNDKAGESLERILLSNSVAKEDLVLLKSKTLPTILKTRI
CCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHECCCCCHHHHHHHH
IASHQQVCRVDREEIVPLTEAEEATILETLKQKIKESSAVILSDYDKGYLTPSLIQSVIS
HHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHH
LCNAEEKIVTVDPQVSHFFLYKNIHIMTPNHHEAGKALGKKLASDSEIESACREISERIT
HHCCCCCEEEECCCCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
PDAMMITRGEKGMSIYERKTNTFYHIPTVAKEVFDVTGAGDTVITTYTAFVASGMSIKEA
CCEEEEECCCCCCCHHHHCCCCEEECHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHH
ALVSNVSAGIVVGKLGAATVTQPEIEDALRTLGYLDEAV
HHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA