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The map label for this gene is tesA [H]

Identifier: 183220370

GI number: 183220370

Start: 1002234

End: 1002896

Strand: Direct

Name: tesA [H]

Synonym: LEPBI_I0966

Alternate gene names: 183220370

Gene position: 1002234-1002896 (Clockwise)

Preceding gene: 183220369

Following gene: 183220371

Centisome position: 27.84

GC content: 41.03

Gene sequence:

>663_bases
ATGCCGTATTTTTTAACCTTTCTTTCTTTATTCATTGCCCTTGGTTGTGGGAAACCTTCCGACCAAACACAAGATCCAAA
AACTCAATCGATAGAATCAAACCAAACCAAACGGATTTTGTATTTTGGTGATTCCTTGACCGCTGGGTATGGCCTAAGCA
ATTATGAAGATGCTTGGCCCCATGTTCTCACAAACCGCATCAATGCGGAAGGTTATTTTTATCAAATGACAAATGCGGGA
GTATCAGGTGATACAACAAGTGGAGGGCTTGGAAGGTTGGAATGGGTGCTTGCGGAAAAACCTTCCATCTTTGTGCTCGA
GTTAGGTGCCAATGATATGTTGCGAGGGATCAGTCCTTCCGTCACAAAAGAAAACTTACGTTCCATGATCCGCCAAATCA
AATCTTTATACCCAAACACCAAAATATTGTTAGTTGGTATGATGGCCACTCCCAATATGGGGAAAAAATATGCGAATGCA
TTCAATTCAATTTATCCAGATCTTGCCAAAGAAGAGAATGTCCCTCTAGTTCCCTTTATCTTAGAAAAAGTGGCCACAAT
TCGAAAACTCAATCAAAAGGATGGAATCCATCCTACAGATGCCGGCCATAAATTGGTAGCGGACACTGTGTACCCTTACA
TAAAACCACTACTAGAAAAATAG

Upstream 100 bases:

>100_bases
ACACTCAAACGTTTGATCCTTGCTTGGATTGTGAAGTTTGGGTGGTGAATTGATTTAAAAATGTTTGATTTTTCCTGAAT
CGTAACCAATCTAATCAGGG

Downstream 100 bases:

>100_bases
GAAACATATCTCATGCAAAGTCCATTCCCCATTCCAACGTTTGCCCTTGAATGTAAAACATTTGCCAAAGATTCGTTTGG
TGCTTCTTTTACCCATCCTT

Product: putative arylesterase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MPYFLTFLSLFIALGCGKPSDQTQDPKTQSIESNQTKRILYFGDSLTAGYGLSNYEDAWPHVLTNRINAEGYFYQMTNAG
VSGDTTSGGLGRLEWVLAEKPSIFVLELGANDMLRGISPSVTKENLRSMIRQIKSLYPNTKILLVGMMATPNMGKKYANA
FNSIYPDLAKEENVPLVPFILEKVATIRKLNQKDGIHPTDAGHKLVADTVYPYIKPLLEK

Sequences:

>Translated_220_residues
MPYFLTFLSLFIALGCGKPSDQTQDPKTQSIESNQTKRILYFGDSLTAGYGLSNYEDAWPHVLTNRINAEGYFYQMTNAG
VSGDTTSGGLGRLEWVLAEKPSIFVLELGANDMLRGISPSVTKENLRSMIRQIKSLYPNTKILLVGMMATPNMGKKYANA
FNSIYPDLAKEENVPLVPFILEKVATIRKLNQKDGIHPTDAGHKLVADTVYPYIKPLLEK
>Mature_219_residues
PYFLTFLSLFIALGCGKPSDQTQDPKTQSIESNQTKRILYFGDSLTAGYGLSNYEDAWPHVLTNRINAEGYFYQMTNAGV
SGDTTSGGLGRLEWVLAEKPSIFVLELGANDMLRGISPSVTKENLRSMIRQIKSLYPNTKILLVGMMATPNMGKKYANAF
NSIYPDLAKEENVPLVPFILEKVATIRKLNQKDGIHPTDAGHKLVADTVYPYIKPLLEK

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=181, Percent_Identity=38.6740331491713, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 24455; Mature: 24324

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYFLTFLSLFIALGCGKPSDQTQDPKTQSIESNQTKRILYFGDSLTAGYGLSNYEDAWP
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
HVLTNRINAEGYFYQMTNAGVSGDTTSGGLGRLEWVLAEKPSIFVLELGANDMLRGISPS
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHCCCCC
VTKENLRSMIRQIKSLYPNTKILLVGMMATPNMGKKYANAFNSIYPDLAKEENVPLVPFI
CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCHHCCCCCCCHHHH
LEKVATIRKLNQKDGIHPTDAGHKLVADTVYPYIKPLLEK
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PYFLTFLSLFIALGCGKPSDQTQDPKTQSIESNQTKRILYFGDSLTAGYGLSNYEDAWP
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
HVLTNRINAEGYFYQMTNAGVSGDTTSGGLGRLEWVLAEKPSIFVLELGANDMLRGISPS
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHCCCCC
VTKENLRSMIRQIKSLYPNTKILLVGMMATPNMGKKYANAFNSIYPDLAKEENVPLVPFI
CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCHHCCCCCCCHHHH
LEKVATIRKLNQKDGIHPTDAGHKLVADTVYPYIKPLLEK
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]