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The map label for this gene is pleD [H]

Identifier: 183219920

GI number: 183219920

Start: 499916

End: 500311

Strand: Reverse

Name: pleD [H]

Synonym: LEPBI_I0502

Alternate gene names: 183219920

Gene position: 500311-499916 (Counterclockwise)

Preceding gene: 183219921

Following gene: 183219918

Centisome position: 13.9

GC content: 39.14

Gene sequence:

>396_bases
ATGAGCATACAGGCCTTAAAACACGTGTTAATCGTGGAAGATGAAGAAGACATTGTCGAAATCCTTAGGATCGCACTTGC
ATTTAACTCAAGTTATGAAGTGAGTTTTGCAAAAACTGGTCCGGAAGGATTACAAAAAGCCATCATCCTGCAACCTGATT
TGATCCTCTTGGATGTACTTATGCCAGGTATGAATGGGATGGAACTCATTGAAGAATTAAAAATTTTCCCAGAGACAAAA
AACATCCCTGTTGCATTTATCACGTCACGCGTGTTAAAGAATGAAATTCTGGAATACCAAAAAAGAGGGGGCATTGGTGT
GATTGAAAAACCCTTTGCCCCTCTTGAAATCTCAGAAAAAATCCAAACCCTATGGGAGGATTTTCATCATCAGTAA

Upstream 100 bases:

>100_bases
TGAATGGGAAAATTTACTTTTTTTCTGATGATTCGGGAACTGTTTTTACCATTGAACTACCAATTGTAATACCAGGACAA
ATTCCTGTTGGGATGAACCA

Downstream 100 bases:

>100_bases
TTTCTTTTTTAAATTACTGATCTTGGAAACCGGTTAAAATTTCTTCTTGGCGGCCCATAAGTTTTACAGATAACCTTGGT
CCCACCTCACAAACGATACG

Product: putative two-component response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MSIQALKHVLIVEDEEDIVEILRIALAFNSSYEVSFAKTGPEGLQKAIILQPDLILLDVLMPGMNGMELIEELKIFPETK
NIPVAFITSRVLKNEILEYQKRGGIGVIEKPFAPLEISEKIQTLWEDFHHQ

Sequences:

>Translated_131_residues
MSIQALKHVLIVEDEEDIVEILRIALAFNSSYEVSFAKTGPEGLQKAIILQPDLILLDVLMPGMNGMELIEELKIFPETK
NIPVAFITSRVLKNEILEYQKRGGIGVIEKPFAPLEISEKIQTLWEDFHHQ
>Mature_130_residues
SIQALKHVLIVEDEEDIVEILRIALAFNSSYEVSFAKTGPEGLQKAIILQPDLILLDVLMPGMNGMELIEELKIFPETKN
IPVAFITSRVLKNEILEYQKRGGIGVIEKPFAPLEISEKIQTLWEDFHHQ

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=32.2033898305085, Blast_Score=59, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 14870; Mature: 14738

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQALKHVLIVEDEEDIVEILRIALAFNSSYEVSFAKTGPEGLQKAIILQPDLILLDVL
CCHHHHHHHHEEECHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHH
MPGMNGMELIEELKIFPETKNIPVAFITSRVLKNEILEYQKRGGIGVIEKPFAPLEISEK
HCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
IQTLWEDFHHQ
HHHHHHHHCCC
>Mature Secondary Structure 
SIQALKHVLIVEDEEDIVEILRIALAFNSSYEVSFAKTGPEGLQKAIILQPDLILLDVL
CHHHHHHHHEEECHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHH
MPGMNGMELIEELKIFPETKNIPVAFITSRVLKNEILEYQKRGGIGVIEKPFAPLEISEK
HCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
IQTLWEDFHHQ
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA