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The map label for this gene is argG

Identifier: 183219514

GI number: 183219514

Start: 86865

End: 88073

Strand: Reverse

Name: argG

Synonym: LEPBI_I0087

Alternate gene names: 183219514

Gene position: 88073-86865 (Counterclockwise)

Preceding gene: 183219518

Following gene: 183219513

Centisome position: 2.45

GC content: 41.77

Gene sequence:

>1209_bases
ATGAAAGAGAAACCTGCTCCCAAAAAAATCGTTCTCGCTTACTCAGGTGGACTCGACACCTCCGTGATCCTTGCTTGGTT
GAAAGATACCTATGGTTGTGAAGTCATCGCTTTTTGTGCCGATGTGGGTCAAAAAGAAGAATTAACAGGCTTAGAAGAAA
AAGGAAAAAACACGGGTGCCTCCAAAGTTTACATCCAAGACCTACGTTTGGAATTTGCACGTGATTTTATTTACCCTGCG
ATCCGCGGAAACGCCATTTATGAAATGCGATATTTACTCGGCACCTCGCTTGCGAGACCACTCATTGCAAAGGCAATGGC
GGATGTAGCCAAAAAAGAAGGCGCTGATGCTTTTTCTCATGGTGCCACTGGAAAAGGAAACGACCAAGTACGATTTGAAC
TCACTTTCAAAGCTCTTTCGCCTAACTTACAAATCATTGCTCCATGGAGGACATGGGATTTTGGTGGTCGAGCTGATCTC
ATAGAATATGCAAAGAAAAAAGGAATCCCAGTTCCAGTAACAGCTGCTAAACCATATAGTATGGATAGAAACCTGATGCA
CCTTTCCTTTGAAGGTGGAATTTTAGAAGACCCATACAACGAACCAAAAGAAGATATGTTTATCCTCACCGTTTCACCTG
AAAAAGCACCTGACAAACCAACGTATTTAGAATTGGATTTTGAAAATGGAGACTGTGTTGCCATTGATGGAAAAAAAATG
AATCCTCTCGAAGTGATGGAAACCTTGAATGATTTAGGCGGAAAGAATGGAGTGGGTCGTGTGGACATCGTGGAAAACAG
ACTTGTTGGGATTAAATCTCGTGGAGTCTATGAAACTCCTGGTGGAACCATTCTTCATATTGCTCATCGTGACCTTGAGT
CCATCACTCTTGACCGCGACACCCAACACAAAAAAGACGAACTCTCCCAAGAGTTTGCTCGATACATTTACAATGGCCAA
TGGTATTCCAACCAAATGAATGCACTCAGAGCCTATATGGACTACACTCAAAAGTATGTGAATGGAACCGTTCGTATCAA
ATTGTACAAAGGTAATTGTACTGTGGTTGGTCGAAAATCCAACAAATCATTGTACAACGCTGGACTTTCTACATTTGAAA
AAGAAGAATTGTACAACCAGTACGATGCAGAAGGTTTCATCAATTTGTATGGCCTTCCAATGAAAGAATGGGCAAGGGTA
AACCAATAA

Upstream 100 bases:

>100_bases
AGGCGACAAAAAAACGCATCCACCAAATCTACTACACAACTTCGTTTCAGACAATATTTAATTGTGTTCTTAGTTTTTTC
TTTTATTTTGACCTTATTCT

Downstream 100 bases:

>100_bases
GATGAAAAGTATCGCCGTATACCCTGGTTCCTTTGATCCATTCACCAATGGACATTTAGATATCATACGGCGAGCCCATC
CACTGTTTGAAGAAATCATC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKEKPAPKKIVLAYSGGLDTSVILAWLKDTYGCEVIAFCADVGQKEELTGLEEKGKNTGASKVYIQDLRLEFARDFIYPA
IRGNAIYEMRYLLGTSLARPLIAKAMADVAKKEGADAFSHGATGKGNDQVRFELTFKALSPNLQIIAPWRTWDFGGRADL
IEYAKKKGIPVPVTAAKPYSMDRNLMHLSFEGGILEDPYNEPKEDMFILTVSPEKAPDKPTYLELDFENGDCVAIDGKKM
NPLEVMETLNDLGGKNGVGRVDIVENRLVGIKSRGVYETPGGTILHIAHRDLESITLDRDTQHKKDELSQEFARYIYNGQ
WYSNQMNALRAYMDYTQKYVNGTVRIKLYKGNCTVVGRKSNKSLYNAGLSTFEKEELYNQYDAEGFINLYGLPMKEWARV
NQ

Sequences:

>Translated_402_residues
MKEKPAPKKIVLAYSGGLDTSVILAWLKDTYGCEVIAFCADVGQKEELTGLEEKGKNTGASKVYIQDLRLEFARDFIYPA
IRGNAIYEMRYLLGTSLARPLIAKAMADVAKKEGADAFSHGATGKGNDQVRFELTFKALSPNLQIIAPWRTWDFGGRADL
IEYAKKKGIPVPVTAAKPYSMDRNLMHLSFEGGILEDPYNEPKEDMFILTVSPEKAPDKPTYLELDFENGDCVAIDGKKM
NPLEVMETLNDLGGKNGVGRVDIVENRLVGIKSRGVYETPGGTILHIAHRDLESITLDRDTQHKKDELSQEFARYIYNGQ
WYSNQMNALRAYMDYTQKYVNGTVRIKLYKGNCTVVGRKSNKSLYNAGLSTFEKEELYNQYDAEGFINLYGLPMKEWARV
NQ
>Mature_402_residues
MKEKPAPKKIVLAYSGGLDTSVILAWLKDTYGCEVIAFCADVGQKEELTGLEEKGKNTGASKVYIQDLRLEFARDFIYPA
IRGNAIYEMRYLLGTSLARPLIAKAMADVAKKEGADAFSHGATGKGNDQVRFELTFKALSPNLQIIAPWRTWDFGGRADL
IEYAKKKGIPVPVTAAKPYSMDRNLMHLSFEGGILEDPYNEPKEDMFILTVSPEKAPDKPTYLELDFENGDCVAIDGKKM
NPLEVMETLNDLGGKNGVGRVDIVENRLVGIKSRGVYETPGGTILHIAHRDLESITLDRDTQHKKDELSQEFARYIYNGQ
WYSNQMNALRAYMDYTQKYVNGTVRIKLYKGNCTVVGRKSNKSLYNAGLSTFEKEELYNQYDAEGFINLYGLPMKEWARV
NQ

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=401, Percent_Identity=48.6284289276808, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI16950633, Length=401, Percent_Identity=48.6284289276808, Blast_Score=382, Evalue=1e-106,
Organism=Escherichia coli, GI1789563, Length=366, Percent_Identity=30.0546448087432, Blast_Score=156, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324514, Length=404, Percent_Identity=47.2772277227723, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21358151, Length=407, Percent_Identity=46.9287469287469, Blast_Score=358, Evalue=4e-99,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_LEPBA (B0S9J6)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961211.1
- ProteinModelPortal:   B0S9J6
- SMR:   B0S9J6
- GeneID:   6387061
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0087
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- ProtClustDB:   PRK00509
- BioCyc:   LBIF355278:LBF_0087-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45184; Mature: 45184

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 40-40 BINDING 91-91 BINDING 96-96 BINDING 121-121 BINDING 123-123 BINDING 127-127 BINDING 127-127 BINDING 128-128 BINDING 131-131 BINDING 180-180 BINDING 189-189 BINDING 265-265 BINDING 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKPAPKKIVLAYSGGLDTSVILAWLKDTYGCEVIAFCADVGQKEELTGLEEKGKNTGA
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCHHHCCCCCCC
SKVYIQDLRLEFARDFIYPAIRGNAIYEMRYLLGTSLARPLIAKAMADVAKKEGADAFSH
CEEEHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
GATGKGNDQVRFELTFKALSPNLQIIAPWRTWDFGGRADLIEYAKKKGIPVPVTAAKPYS
CCCCCCCCEEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEECCCCCC
MDRNLMHLSFEGGILEDPYNEPKEDMFILTVSPEKAPDKPTYLELDFENGDCVAIDGKKM
CCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEECCCCC
NPLEVMETLNDLGGKNGVGRVDIVENRLVGIKSRGVYETPGGTILHIAHRDLESITLDRD
CHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCEEECCCCCEEEEECCCCHHEECCCC
TQHKKDELSQEFARYIYNGQWYSNQMNALRAYMDYTQKYVNGTVRIKLYKGNCTVVGRKS
CCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEECC
NKSLYNAGLSTFEKEELYNQYDAEGFINLYGLPMKEWARVNQ
CCHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCC
>Mature Secondary Structure
MKEKPAPKKIVLAYSGGLDTSVILAWLKDTYGCEVIAFCADVGQKEELTGLEEKGKNTGA
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCHHHCCCCCCC
SKVYIQDLRLEFARDFIYPAIRGNAIYEMRYLLGTSLARPLIAKAMADVAKKEGADAFSH
CEEEHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
GATGKGNDQVRFELTFKALSPNLQIIAPWRTWDFGGRADLIEYAKKKGIPVPVTAAKPYS
CCCCCCCCEEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEECCCCCC
MDRNLMHLSFEGGILEDPYNEPKEDMFILTVSPEKAPDKPTYLELDFENGDCVAIDGKKM
CCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEECCCCC
NPLEVMETLNDLGGKNGVGRVDIVENRLVGIKSRGVYETPGGTILHIAHRDLESITLDRD
CHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCEEECCCCCEEEEECCCCHHEECCCC
TQHKKDELSQEFARYIYNGQWYSNQMNALRAYMDYTQKYVNGTVRIKLYKGNCTVVGRKS
CCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEECC
NKSLYNAGLSTFEKEELYNQYDAEGFINLYGLPMKEWARVNQ
CCHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA