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The map label for this gene is dnaN [H]

Identifier: 183219429

GI number: 183219429

Start: 1713

End: 2831

Strand: Direct

Name: dnaN [H]

Synonym: LEPBI_I0002

Alternate gene names: 183219429

Gene position: 1713-2831 (Clockwise)

Preceding gene: 183219428

Following gene: 183219430

Centisome position: 0.05

GC content: 36.46

Gene sequence:

>1119_bases
ATGAAATTCACTGTCAATACTACAGAATTCCTAAAAGCAATTAACTCAGTGGATGGAGTGATCTCAGTACGTGAGATCAA
GTCGGCTCTTTCCAATTTAAAAATCCAAACAGGCGAAAACGAAGTGTATCTTTCAGCGACTGATTTAGAGATCGCAATCA
AAACTTCGGTTCCTTCTACCATTGGAGAAAAAGGAACAGCATCACTTCCTGCAAAACAGCTCTCTAGTATTTTTAAAAAT
TTAAACTTTGATACAAGTTTGCTGACGACAACGGAAAATTCTGAGAATTCAGAAACCACAATCACTGATGCTTCTGGGAA
AATGGACACAAAGTTCAAAGTGAATGGAATCGATTCAGAAGACATCAAAACCATTCCGAAAGTAGATGAGGCAAGTGTGG
TTGAATTTCCTTGCCAAACCATTCGTGAAATGTTCCGTAAAACTTCATATGCAATGGCAATTGAAGAAACTCGATTTGTA
TTCAATGGTTTGTTTTTAAAACCAGATAACACGGATTTAATTGTTGTAGGAACCGATGGGCGTCGTTTATCAAAAATTGT
TCGTAAGTTTCCAAAACAATTTCCATTTAAAAATGGTGTGATCATTCCTCACAAAGCAGTGCGTGAGATGCTTAAAATGA
TGGAAGGAAAAGAAACAGCAAAAATTGGTTTTGTGGAAGAACAAATTTATGTTTCTTCTGGAAACGTAGAACTCCTATTT
AAATTAATAGATGGAAATTTTCCTGATTATGAACAAGTGATTCCAAAACAAACAACCGAATCTGTTCGAGTGGTGAAAGC
AGACTTCCTTACATTTTTAAAACAAGCTTTGATCTCAGCCGAAGAACCATCGAAACAAATTCGTTTGGCTTTTAGCAAAG
GTAATGTAAATATTAGTTCTTCGAATCCAGGAACGATGATGTTTGATCACAACATGCCGATTGAATACAATGGAGAAACA
ATCACCATTGCTTTCAAAGGAGATTATTTGAGTGATGTTGTGAAAGCTGTGGATGACCCAGAAGTGATTTTGGAATTCAC
CACTTCAAGTGCTCCCGTACTGTTTAAGGATCCTTCGGACAGTGACTTTGTTTCTGTCATTATGCCAATGAAACTTTAA

Upstream 100 bases:

>100_bases
AAAATTGAACTTTCCGGGCTTTATCAACATACTGACAGAACCAACGGAAACGACATAATATATATAAAGATATATAATAT
TAATAAGAAGAAGAGGAAAA

Downstream 100 bases:

>100_bases
TGTTTCTAAAAAAGATTTACATAAAGAATTTTCGTAACCACGAAGAAACTCAACTTACATTTAAATCACGTCTTGTCTTC
TTCATTGGTAACAATGGGGA

Product: DNA polymerase IIIsubunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKFTVNTTEFLKAINSVDGVISVREIKSALSNLKIQTGENEVYLSATDLEIAIKTSVPSTIGEKGTASLPAKQLSSIFKN
LNFDTSLLTTTENSENSETTITDASGKMDTKFKVNGIDSEDIKTIPKVDEASVVEFPCQTIREMFRKTSYAMAIEETRFV
FNGLFLKPDNTDLIVVGTDGRRLSKIVRKFPKQFPFKNGVIIPHKAVREMLKMMEGKETAKIGFVEEQIYVSSGNVELLF
KLIDGNFPDYEQVIPKQTTESVRVVKADFLTFLKQALISAEEPSKQIRLAFSKGNVNISSSNPGTMMFDHNMPIEYNGET
ITIAFKGDYLSDVVKAVDDPEVILEFTTSSAPVLFKDPSDSDFVSVIMPMKL

Sequences:

>Translated_372_residues
MKFTVNTTEFLKAINSVDGVISVREIKSALSNLKIQTGENEVYLSATDLEIAIKTSVPSTIGEKGTASLPAKQLSSIFKN
LNFDTSLLTTTENSENSETTITDASGKMDTKFKVNGIDSEDIKTIPKVDEASVVEFPCQTIREMFRKTSYAMAIEETRFV
FNGLFLKPDNTDLIVVGTDGRRLSKIVRKFPKQFPFKNGVIIPHKAVREMLKMMEGKETAKIGFVEEQIYVSSGNVELLF
KLIDGNFPDYEQVIPKQTTESVRVVKADFLTFLKQALISAEEPSKQIRLAFSKGNVNISSSNPGTMMFDHNMPIEYNGET
ITIAFKGDYLSDVVKAVDDPEVILEFTTSSAPVLFKDPSDSDFVSVIMPMKL
>Mature_372_residues
MKFTVNTTEFLKAINSVDGVISVREIKSALSNLKIQTGENEVYLSATDLEIAIKTSVPSTIGEKGTASLPAKQLSSIFKN
LNFDTSLLTTTENSENSETTITDASGKMDTKFKVNGIDSEDIKTIPKVDEASVVEFPCQTIREMFRKTSYAMAIEETRFV
FNGLFLKPDNTDLIVVGTDGRRLSKIVRKFPKQFPFKNGVIIPHKAVREMLKMMEGKETAKIGFVEEQIYVSSGNVELLF
KLIDGNFPDYEQVIPKQTTESVRVVKADFLTFLKQALISAEEPSKQIRLAFSKGNVNISSSNPGTMMFDHNMPIEYNGET
ITIAFKGDYLSDVVKAVDDPEVILEFTTSSAPVLFKDPSDSDFVSVIMPMKL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=378, Percent_Identity=27.2486772486773, Blast_Score=144, Evalue=7e-36,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41317; Mature: 41317

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTVNTTEFLKAINSVDGVISVREIKSALSNLKIQTGENEVYLSATDLEIAIKTSVPST
CEEECCHHHHHHHHHHHCCEEEHHHHHHHHHCCEEEECCCEEEEEEECEEEEEEECCCHH
IGEKGTASLPAKQLSSIFKNLNFDTSLLTTTENSENSETTITDASGKMDTKFKVNGIDSE
HCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEECCCCHH
DIKTIPKVDEASVVEFPCQTIREMFRKTSYAMAIEETRFVFNGLFLKPDNTDLIVVGTDG
HHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHEEHHHHEEEHEEEEECCCCCCEEEECCCC
RRLSKIVRKFPKQFPFKNGVIIPHKAVREMLKMMEGKETAKIGFVEEQIYVSSGNVELLF
HHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCEEEE
KLIDGNFPDYEQVIPKQTTESVRVVKADFLTFLKQALISAEEPSKQIRLAFSKGNVNISS
EEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEC
SNPGTMMFDHNMPIEYNGETITIAFKGDYLSDVVKAVDDPEVILEFTTSSAPVLFKDPSD
CCCCEEEEECCCCEEECCCEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCEEEECCCC
SDFVSVIMPMKL
CCEEEEEEECCC
>Mature Secondary Structure
MKFTVNTTEFLKAINSVDGVISVREIKSALSNLKIQTGENEVYLSATDLEIAIKTSVPST
CEEECCHHHHHHHHHHHCCEEEHHHHHHHHHCCEEEECCCEEEEEEECEEEEEEECCCHH
IGEKGTASLPAKQLSSIFKNLNFDTSLLTTTENSENSETTITDASGKMDTKFKVNGIDSE
HCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEECCCCHH
DIKTIPKVDEASVVEFPCQTIREMFRKTSYAMAIEETRFVFNGLFLKPDNTDLIVVGTDG
HHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHEEHHHHEEEHEEEEECCCCCCEEEECCCC
RRLSKIVRKFPKQFPFKNGVIIPHKAVREMLKMMEGKETAKIGFVEEQIYVSSGNVELLF
HHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCEEEE
KLIDGNFPDYEQVIPKQTTESVRVVKADFLTFLKQALISAEEPSKQIRLAFSKGNVNISS
EEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEC
SNPGTMMFDHNMPIEYNGETITIAFKGDYLSDVVKAVDDPEVILEFTTSSAPVLFKDPSD
CCCCEEEEECCCCEEECCCEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCEEEECCCC
SDFVSVIMPMKL
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9784136 [H]