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The map label for this gene is dnaA

Identifier: 183219428

GI number: 183219428

Start: 141

End: 1466

Strand: Direct

Name: dnaA

Synonym: LEPBI_I0001

Alternate gene names: 183219428

Gene position: 141-1466 (Clockwise)

Preceding gene: NA

Following gene: 183219429

Centisome position: 0.0

GC content: 39.44

Gene sequence:

>1326_bases
TTGGACATACGTTGGGAAGAAATTTTAGAAGAAATATCGAAACAGATACCTCCCAAGTACTTTTCCAATTTCATTGCACC
ACTTCGATTTGATAAGTGGGAGAACCAAGTGGTTCACTTAATGGCTCCATCGGGTGGAATCAAACGCCATGTGGAAACCA
AATACATCGGTTTCATTGAAGATGCGGTTTACCAAGTCGTAGGGGATCGTTTCAAAGTATCCATTCTGACTGAATCGGAA
ACATCATCACATGTTTTAAAAGAAGTCATCCAATCGAAGTTTGATGATTCAGACTCAGATCTCAATCCAGAATATATCTT
TAGCAATTATATCACTTCTGATTCCAACCGAATTGCGTTCACAGCAGCCAAATCAGTGGTGGAACAACCTGGTAAGTACA
ATCCTTTGTATTTGTTTGGACCCGTTGGTGTGGGCAAAACACATCTTCTTCATTCCATAGGTAACGAGATCAAAAAAAAA
GATCCATGGAAAACAGTTCGTTATGTCAATAGTACATCTTTTTTGAATGAGTTTATTTTTACCGTAAGACAAAACAATCG
TGAGTCTCTCGAATCTTTTAAAATCCGTTACCAGTCTTATAATGTTTTACTCTTTGATGACATTCAGTTTTTAAATGGTG
GTGCCGAAAAAACCCAGGAAGAGTTTTTTGCTCTTTTTAATTTTTTGTACGATCGCAAACGCCAAATCGTCATCGCATCG
GATCGCCCAAGTTATGAACTCCCACTCCATGACCGACTCAAATCTCGGTTTGTACATGGTTTGCAAGCAGATATCAAATC
GCATGATTTGGAACTACGAAAATCATTACTCAAATCCAATTTCTCTGAATTCAATATCCCTGCCAGTGACAATTTACTCC
ATTGGCTCGCCGAACGATTAGAAGGTGATTCAAGAGCCCTCATTGGGATTGTAAACGATTTGGTAATGTACAAAAAAGCC
TATGAGTATTTCCTACTCACGGAAGACAAAATCAAAGAGATTGCCGAAGCACGTTTCCTCACGAATAAAAAACGAATTGG
ATTCAGTCCTGATATGGTAATCGATTTGGTCTGTGAACGGACGAATGTGGCGAGAAAGGATTTACTAGGAAAAAGCCGCA
AAGCCGATTTCATCCCTCCGCGACACCTTTGTATGTTACTGTTACACGATGTCTTGAATGTTCCGAAAGCCCAAATCGGA
AGGATTTTTTCGACGACACATTCGACTGTGATCCATGGGATCGATAAATTTAAAGAGCGGATGAAATCGGAAGCACAATG
GGAAGATCTTTTCCACACCATCAAACACAAGATTAGCTTTCAATAA

Upstream 100 bases:

>100_bases
GACTATTTTTAACAGTTTGGATCGTTTTCCGATTTTTCATTTGAATTCTACCTACTTGCTAAAAGTATAGTACCCGATTT
CACATTTAGGGCGGGGAAGC

Downstream 100 bases:

>100_bases
TCTGTTACTAAGACCAAGATAGACTGTCAATAAACTGTCGATAGGACATAGTCACGGGTTATGGTTCCAATATTGACGAG
TAAACTGTTCAAAGGGACAT

Product: chromosomal replication initiator protein DnaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MDIRWEEILEEISKQIPPKYFSNFIAPLRFDKWENQVVHLMAPSGGIKRHVETKYIGFIEDAVYQVVGDRFKVSILTESE
TSSHVLKEVIQSKFDDSDSDLNPEYIFSNYITSDSNRIAFTAAKSVVEQPGKYNPLYLFGPVGVGKTHLLHSIGNEIKKK
DPWKTVRYVNSTSFLNEFIFTVRQNNRESLESFKIRYQSYNVLLFDDIQFLNGGAEKTQEEFFALFNFLYDRKRQIVIAS
DRPSYELPLHDRLKSRFVHGLQADIKSHDLELRKSLLKSNFSEFNIPASDNLLHWLAERLEGDSRALIGIVNDLVMYKKA
YEYFLLTEDKIKEIAEARFLTNKKRIGFSPDMVIDLVCERTNVARKDLLGKSRKADFIPPRHLCMLLLHDVLNVPKAQIG
RIFSTTHSTVIHGIDKFKERMKSEAQWEDLFHTIKHKISFQ

Sequences:

>Translated_441_residues
MDIRWEEILEEISKQIPPKYFSNFIAPLRFDKWENQVVHLMAPSGGIKRHVETKYIGFIEDAVYQVVGDRFKVSILTESE
TSSHVLKEVIQSKFDDSDSDLNPEYIFSNYITSDSNRIAFTAAKSVVEQPGKYNPLYLFGPVGVGKTHLLHSIGNEIKKK
DPWKTVRYVNSTSFLNEFIFTVRQNNRESLESFKIRYQSYNVLLFDDIQFLNGGAEKTQEEFFALFNFLYDRKRQIVIAS
DRPSYELPLHDRLKSRFVHGLQADIKSHDLELRKSLLKSNFSEFNIPASDNLLHWLAERLEGDSRALIGIVNDLVMYKKA
YEYFLLTEDKIKEIAEARFLTNKKRIGFSPDMVIDLVCERTNVARKDLLGKSRKADFIPPRHLCMLLLHDVLNVPKAQIG
RIFSTTHSTVIHGIDKFKERMKSEAQWEDLFHTIKHKISFQ
>Mature_441_residues
MDIRWEEILEEISKQIPPKYFSNFIAPLRFDKWENQVVHLMAPSGGIKRHVETKYIGFIEDAVYQVVGDRFKVSILTESE
TSSHVLKEVIQSKFDDSDSDLNPEYIFSNYITSDSNRIAFTAAKSVVEQPGKYNPLYLFGPVGVGKTHLLHSIGNEIKKK
DPWKTVRYVNSTSFLNEFIFTVRQNNRESLESFKIRYQSYNVLLFDDIQFLNGGAEKTQEEFFALFNFLYDRKRQIVIAS
DRPSYELPLHDRLKSRFVHGLQADIKSHDLELRKSLLKSNFSEFNIPASDNLLHWLAERLEGDSRALIGIVNDLVMYKKA
YEYFLLTEDKIKEIAEARFLTNKKRIGFSPDMVIDLVCERTNVARKDLLGKSRKADFIPPRHLCMLLLHDVLNVPKAQIG
RIFSTTHSTVIHGIDKFKERMKSEAQWEDLFHTIKHKISFQ

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=469, Percent_Identity=29.8507462686567, Blast_Score=186, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_LEPBA (B0S907)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961129.1
- ProteinModelPortal:   B0S907
- GeneID:   6388583
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0004
- HOGENOM:   HBG695666
- OMA:   DEVAFLI
- ProtClustDB:   PRK00149
- BioCyc:   LBIF355278:LBF_0004-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 51326; Mature: 51326

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRWEEILEEISKQIPPKYFSNFIAPLRFDKWENQVVHLMAPSGGIKRHVETKYIGFIE
CCCCHHHHHHHHHHHCCHHHHHHHHCCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
DAVYQVVGDRFKVSILTESETSSHVLKEVIQSKFDDSDSDLNPEYIFSNYITSDSNRIAF
HHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEECCCCEEEH
TAAKSVVEQPGKYNPLYLFGPVGVGKTHLLHSIGNEIKKKDPWKTVRYVNSTSFLNEFIF
HHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHH
TVRQNNRESLESFKIRYQSYNVLLFDDIQFLNGGAEKTQEEFFALFNFLYDRKRQIVIAS
HHHCCCHHHHHHHHHHHEECCEEEEECHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEC
DRPSYELPLHDRLKSRFVHGLQADIKSHDLELRKSLLKSNFSEFNIPASDNLLHWLAERL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
EGDSRALIGIVNDLVMYKKAYEYFLLTEDKIKEIAEARFLTNKKRIGFSPDMVIDLVCER
CCCCHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
TNVARKDLLGKSRKADFIPPRHLCMLLLHDVLNVPKAQIGRIFSTTHSTVIHGIDKFKER
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MKSEAQWEDLFHTIKHKISFQ
HHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIRWEEILEEISKQIPPKYFSNFIAPLRFDKWENQVVHLMAPSGGIKRHVETKYIGFIE
CCCCHHHHHHHHHHHCCHHHHHHHHCCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
DAVYQVVGDRFKVSILTESETSSHVLKEVIQSKFDDSDSDLNPEYIFSNYITSDSNRIAF
HHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEECCCCEEEH
TAAKSVVEQPGKYNPLYLFGPVGVGKTHLLHSIGNEIKKKDPWKTVRYVNSTSFLNEFIF
HHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHH
TVRQNNRESLESFKIRYQSYNVLLFDDIQFLNGGAEKTQEEFFALFNFLYDRKRQIVIAS
HHHCCCHHHHHHHHHHHEECCEEEEECHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEC
DRPSYELPLHDRLKSRFVHGLQADIKSHDLELRKSLLKSNFSEFNIPASDNLLHWLAERL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
EGDSRALIGIVNDLVMYKKAYEYFLLTEDKIKEIAEARFLTNKKRIGFSPDMVIDLVCER
CCCCHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
TNVARKDLLGKSRKADFIPPRHLCMLLLHDVLNVPKAQIGRIFSTTHSTVIHGIDKFKER
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MKSEAQWEDLFHTIKHKISFQ
HHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA