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The map label for this gene is recF

Identifier: 183219430

GI number: 183219430

Start: 2831

End: 3934

Strand: Direct

Name: recF

Synonym: LEPBI_I0003

Alternate gene names: 183219430

Gene position: 2831-3934 (Clockwise)

Preceding gene: 183219429

Following gene: 183219431

Centisome position: 0.08

GC content: 35.69

Gene sequence:

>1104_bases
ATGTTTCTAAAAAAGATTTACATAAAGAATTTTCGTAACCACGAAGAAACTCAACTTACATTTAAATCACGTCTTGTCTT
CTTCATTGGTAACAATGGGGAAGGTAAGACGAACTTACTCGAATCCATTTCTCTCTTATCATACTTAAAAAGTTTTCGTG
AATCAGACCAAAACCAATTGTTGCGTTGGGATACTTCTGATACTTTTATCAGAGCAGAATTTGAATCCGAAGGGAATGAA
TATTTATTCGAATACGGAATTGAACATTCCCAAACGAAACGAAAAAAATTAAAAGTGAATGGGGAAGAATTCAAAAAAAT
TTCTGACTATGTTGGATACTTTCGCTCCATAGTGATGAGCCCTCCAGACATCCTCATCATAGAAGATGGAAATGTGGAAC
GTAGACGATTTTTAGATGCATTTATCTCTTCCACAAATCGTTATTATTTAAAACAACTGATTGAATATGAAAGGTTAATT
AAACAAAGGAATGCGGCTCTCAAAAAAGAAAATGCCAGTGATCGAGAAATTGGCATTTGGGATGAACCGATCATCGAACA
CGATTCGGAAATCAGAGAGATCCGAACTAAAACAATCCAAAGTTTGGCTGGATATTTCCATCAAAATTTATTACAACTCA
GTTCCGGAAAAGATCCATATTTTCTCACATACAAACCTAATATCACTTCGAAAGAAGAACACAAACAAAAGTTAATCGAT
AATTTACGAAAAGATAAAGCGATCGGGTATACAAGTTGTGGAAACCATCGAGACACACTTCCTATTGGATTTGATGACAA
AGACTTGAGTGGGTTTGGATCCCAAGGCCAAAAACGAAGTGCTGTGATTGCCTTAAAGACTGCTTGTTTTCAAATGATTC
GAGACACAACTGGAGAAGCTCCCGTTTTACTCATTGATGATATTATCCGTGAACTCGATGTCAAACGAAGGGAGTATTTC
GTAAATTTGATCTCTGAATGTGGACAGGCATTTTTTACCACCACAGATTTGGAAGGAATCAATGAGTATGTGGGAAACCT
AACTGTTGATAAAGAAATTTACATCATTGATTCAGGAAAAGTGAAGGTCTTTACTGAGATATGA

Upstream 100 bases:

>100_bases
CAGAAGTGATTTTGGAATTCACCACTTCAAGTGCTCCCGTACTGTTTAAGGATCCTTCGGACAGTGACTTTGTTTCTGTC
ATTATGCCAATGAAACTTTA

Downstream 100 bases:

>100_bases
AAAAAGTCGAACTTTCAGAACTCTTCCAAAGTTTAGAAAAATTAGGTCTCGATCGAGAATCAGTCTTTCAAGACCAAATT
TTAAAAAAACTAAGGAACCA

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MFLKKIYIKNFRNHEETQLTFKSRLVFFIGNNGEGKTNLLESISLLSYLKSFRESDQNQLLRWDTSDTFIRAEFESEGNE
YLFEYGIEHSQTKRKKLKVNGEEFKKISDYVGYFRSIVMSPPDILIIEDGNVERRRFLDAFISSTNRYYLKQLIEYERLI
KQRNAALKKENASDREIGIWDEPIIEHDSEIREIRTKTIQSLAGYFHQNLLQLSSGKDPYFLTYKPNITSKEEHKQKLID
NLRKDKAIGYTSCGNHRDTLPIGFDDKDLSGFGSQGQKRSAVIALKTACFQMIRDTTGEAPVLLIDDIIRELDVKRREYF
VNLISECGQAFFTTTDLEGINEYVGNLTVDKEIYIIDSGKVKVFTEI

Sequences:

>Translated_367_residues
MFLKKIYIKNFRNHEETQLTFKSRLVFFIGNNGEGKTNLLESISLLSYLKSFRESDQNQLLRWDTSDTFIRAEFESEGNE
YLFEYGIEHSQTKRKKLKVNGEEFKKISDYVGYFRSIVMSPPDILIIEDGNVERRRFLDAFISSTNRYYLKQLIEYERLI
KQRNAALKKENASDREIGIWDEPIIEHDSEIREIRTKTIQSLAGYFHQNLLQLSSGKDPYFLTYKPNITSKEEHKQKLID
NLRKDKAIGYTSCGNHRDTLPIGFDDKDLSGFGSQGQKRSAVIALKTACFQMIRDTTGEAPVLLIDDIIRELDVKRREYF
VNLISECGQAFFTTTDLEGINEYVGNLTVDKEIYIIDSGKVKVFTEI
>Mature_367_residues
MFLKKIYIKNFRNHEETQLTFKSRLVFFIGNNGEGKTNLLESISLLSYLKSFRESDQNQLLRWDTSDTFIRAEFESEGNE
YLFEYGIEHSQTKRKKLKVNGEEFKKISDYVGYFRSIVMSPPDILIIEDGNVERRRFLDAFISSTNRYYLKQLIEYERLI
KQRNAALKKENASDREIGIWDEPIIEHDSEIREIRTKTIQSLAGYFHQNLLQLSSGKDPYFLTYKPNITSKEEHKQKLID
NLRKDKAIGYTSCGNHRDTLPIGFDDKDLSGFGSQGQKRSAVIALKTACFQMIRDTTGEAPVLLIDDIIRELDVKRREYF
VNLISECGQAFFTTTDLEGINEYVGNLTVDKEIYIIDSGKVKVFTEI

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=365, Percent_Identity=23.5616438356164, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_LEPBA (B0S909)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961131.1
- GeneID:   6388670
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0006
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK573281
- BioCyc:   LBIF355278:LBF_0006-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42701; Mature: 42701

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLKKIYIKNFRNHEETQLTFKSRLVFFIGNNGEGKTNLLESISLLSYLKSFRESDQNQL
CCHHHHHHHHCCCCHHHHEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
LRWDTSDTFIRAEFESEGNEYLFEYGIEHSQTKRKKLKVNGEEFKKISDYVGYFRSIVMS
EEECCCCCEEEEEECCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
PPDILIIEDGNVERRRFLDAFISSTNRYYLKQLIEYERLIKQRNAALKKENASDREIGIW
CCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DEPIIEHDSEIREIRTKTIQSLAGYFHQNLLQLSSGKDPYFLTYKPNITSKEEHKQKLID
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHH
NLRKDKAIGYTSCGNHRDTLPIGFDDKDLSGFGSQGQKRSAVIALKTACFQMIRDTTGEA
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
PVLLIDDIIRELDVKRREYFVNLISECGQAFFTTTDLEGINEYVGNLTVDKEIYIIDSGK
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCEEECCEEEEEECCC
VKVFTEI
EEEEEEC
>Mature Secondary Structure
MFLKKIYIKNFRNHEETQLTFKSRLVFFIGNNGEGKTNLLESISLLSYLKSFRESDQNQL
CCHHHHHHHHCCCCHHHHEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
LRWDTSDTFIRAEFESEGNEYLFEYGIEHSQTKRKKLKVNGEEFKKISDYVGYFRSIVMS
EEECCCCCEEEEEECCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
PPDILIIEDGNVERRRFLDAFISSTNRYYLKQLIEYERLIKQRNAALKKENASDREIGIW
CCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DEPIIEHDSEIREIRTKTIQSLAGYFHQNLLQLSSGKDPYFLTYKPNITSKEEHKQKLID
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHH
NLRKDKAIGYTSCGNHRDTLPIGFDDKDLSGFGSQGQKRSAVIALKTACFQMIRDTTGEA
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
PVLLIDDIIRELDVKRREYFVNLISECGQAFFTTTDLEGINEYVGNLTVDKEIYIIDSGK
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCEEECCEEEEEECCC
VKVFTEI
EEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA