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The map label for this gene is yusZ [H]

Identifier: 182677576

GI number: 182677576

Start: 644655

End: 645548

Strand: Direct

Name: yusZ [H]

Synonym: Bind_0581

Alternate gene names: 182677576

Gene position: 644655-645548 (Clockwise)

Preceding gene: 182677575

Following gene: 182677587

Centisome position: 15.46

GC content: 63.98

Gene sequence:

>894_bases
ATGGGACAGGTTATCGTCATCACCGGCGCTTCCAGCGGCTTCGGAGCGCTGACGGCTCGAGCCCTCGCCGATGCCGGGCA
TATTGTCTATGCCGGTATGCGCGACACCGCCGGACGCAATGCGCCGCAGGTCGAAGCCGCCAAAGCTTATGCGAAAGAAC
GCGCCGTCGATCTCCATACGGTCGAGATGGATGTTCAATCCGAAGCGTCCGTTGAGGCCGCGATCGCGACGATTCTGCGA
GAGAACAGCCGGCTCGACACTTTGGTCCATAACGCCGGGCACATGGTGTTTGGCCCGGCCGAGGCCTTCACTCCGGAACA
ATATGCTCAGCTCTATGATGTGAACGTGCTGGGCACCCAACGCGTCAACCGCGCCGCGCTGCCGATCCTGAGACGGCAAG
GCAAGGGTCTGCTCGTGTGGGTCTCCTCGACCAGCACGCGCGGCGGCACACCACCATGGCTTGCGCCCTATTTTGCTGCC
AAGGCGGCGATGGATTCGCTGGCGGTCAGCTATGCCGGCGAACTGGCGCGCTGGGGAATCGAGACCTCGATCATTGTGCC
CGGTGCCTTCACGAAGGGTACCAGCCACTTCGCCCATGCTGGTGCCCCCGCTGATACGGCGAGGGCAAAAGACTATGCCA
ACGGGCCGACGGCGGATATTCCCGATGTCGCCATGAAGGGGTTGAGCGCGCTCGAACCGGCTGATGCCGACGCGGGTGAG
GTGGCTAAGGCGATCACGCGGGTCGTGGACTTGCCGTTTGGCAGGAGGCCGTTTCGGGTCCACATCGATCCCTCGCAGGA
TGGTGCGGAGATCGTCAACGGCGTGGCGGACCGCGTCCGGGCCGAGTTGCTGCGGCGGATCGGCCTGGAGGACATCCTGC
ATCCGCGTGCATGA

Upstream 100 bases:

>100_bases
CCGCCGTCGCGTCGCTGCTGACCGAGGACAATCGCTGGATCAACGCCCAGCGGATCGAAGTCTCCGGCGGCATGTTCGTG
TGATCGAGAGGGGGACCGAC

Downstream 100 bases:

>100_bases
AAATAGCAGCGACAGGACGGCCCATTTTCCGAGTCCGTCCTGTCATTCCCGACCCTCTTATAAATCCTTGTAATCGCTCA
GCCAGCCCTGGACATTGATC

Product: short-chain dehydrogenase/reductase SDR

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: ORFA [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MGQVIVITGASSGFGALTARALADAGHIVYAGMRDTAGRNAPQVEAAKAYAKERAVDLHTVEMDVQSEASVEAAIATILR
ENSRLDTLVHNAGHMVFGPAEAFTPEQYAQLYDVNVLGTQRVNRAALPILRRQGKGLLVWVSSTSTRGGTPPWLAPYFAA
KAAMDSLAVSYAGELARWGIETSIIVPGAFTKGTSHFAHAGAPADTARAKDYANGPTADIPDVAMKGLSALEPADADAGE
VAKAITRVVDLPFGRRPFRVHIDPSQDGAEIVNGVADRVRAELLRRIGLEDILHPRA

Sequences:

>Translated_297_residues
MGQVIVITGASSGFGALTARALADAGHIVYAGMRDTAGRNAPQVEAAKAYAKERAVDLHTVEMDVQSEASVEAAIATILR
ENSRLDTLVHNAGHMVFGPAEAFTPEQYAQLYDVNVLGTQRVNRAALPILRRQGKGLLVWVSSTSTRGGTPPWLAPYFAA
KAAMDSLAVSYAGELARWGIETSIIVPGAFTKGTSHFAHAGAPADTARAKDYANGPTADIPDVAMKGLSALEPADADAGE
VAKAITRVVDLPFGRRPFRVHIDPSQDGAEIVNGVADRVRAELLRRIGLEDILHPRA
>Mature_296_residues
GQVIVITGASSGFGALTARALADAGHIVYAGMRDTAGRNAPQVEAAKAYAKERAVDLHTVEMDVQSEASVEAAIATILRE
NSRLDTLVHNAGHMVFGPAEAFTPEQYAQLYDVNVLGTQRVNRAALPILRRQGKGLLVWVSSTSTRGGTPPWLAPYFAAK
AAMDSLAVSYAGELARWGIETSIIVPGAFTKGTSHFAHAGAPADTARAKDYANGPTADIPDVAMKGLSALEPADADAGEV
AKAITRVVDLPFGRRPFRVHIDPSQDGAEIVNGVADRVRAELLRRIGLEDILHPRA

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI223718074, Length=190, Percent_Identity=37.8947368421053, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI44680136, Length=202, Percent_Identity=30.1980198019802, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI44680133, Length=202, Percent_Identity=30.1980198019802, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI17738292, Length=202, Percent_Identity=30.1980198019802, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI4504503, Length=192, Percent_Identity=30.7291666666667, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI7657478, Length=187, Percent_Identity=30.4812834224599, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI119392083, Length=190, Percent_Identity=31.0526315789474, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI150247226, Length=197, Percent_Identity=33.502538071066, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786812, Length=189, Percent_Identity=31.7460317460317, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787820, Length=216, Percent_Identity=27.3148148148148, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082100, Length=188, Percent_Identity=30.3191489361702, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786701, Length=189, Percent_Identity=30.1587301587302, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788459, Length=194, Percent_Identity=29.3814432989691, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI2367175, Length=212, Percent_Identity=30.188679245283, Blast_Score=61, Evalue=9e-11,
Organism=Escherichia coli, GI87082160, Length=191, Percent_Identity=25.6544502617801, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17557780, Length=197, Percent_Identity=33.502538071066, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115534660, Length=193, Percent_Identity=35.2331606217617, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994604, Length=186, Percent_Identity=31.7204301075269, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=193, Percent_Identity=30.5699481865285, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=233, Percent_Identity=29.1845493562232, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=197, Percent_Identity=30.4568527918782, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24651139, Length=193, Percent_Identity=31.6062176165803, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357041, Length=189, Percent_Identity=32.8042328042328, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571526, Length=190, Percent_Identity=34.7368421052632, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24644339, Length=229, Percent_Identity=30.1310043668122, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI23397609, Length=188, Percent_Identity=31.9148936170213, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 31411; Mature: 31280

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQVIVITGASSGFGALTARALADAGHIVYAGMRDTAGRNAPQVEAAKAYAKERAVDLHT
CCCEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCEEEE
VEMDVQSEASVEAAIATILRENSRLDTLVHNAGHMVFGPAEAFTPEQYAQLYDVNVLGTQ
EEECCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCHHHCCHHHHHHHHCCEEECCH
RVNRAALPILRRQGKGLLVWVSSTSTRGGTPPWLAPYFAAKAAMDSLAVSYAGELARWGI
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETSIIVPGAFTKGTSHFAHAGAPADTARAKDYANGPTADIPDVAMKGLSALEPADADAGE
CEEEEECCCCCCCCCHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHH
VAKAITRVVDLPFGRRPFRVHIDPSQDGAEIVNGVADRVRAELLRRIGLEDILHPRA
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCC
>Mature Secondary Structure 
GQVIVITGASSGFGALTARALADAGHIVYAGMRDTAGRNAPQVEAAKAYAKERAVDLHT
CCEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCEEEE
VEMDVQSEASVEAAIATILRENSRLDTLVHNAGHMVFGPAEAFTPEQYAQLYDVNVLGTQ
EEECCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCHHHCCHHHHHHHHCCEEECCH
RVNRAALPILRRQGKGLLVWVSSTSTRGGTPPWLAPYFAAKAAMDSLAVSYAGELARWGI
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETSIIVPGAFTKGTSHFAHAGAPADTARAKDYANGPTADIPDVAMKGLSALEPADADAGE
CEEEEECCCCCCCCCHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHH
VAKAITRVVDLPFGRRPFRVHIDPSQDGAEIVNGVADRVRAELLRRIGLEDILHPRA
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 8396117 [H]