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The map label for this gene is ykoU [H]

Identifier: 182677378

GI number: 182677378

Start: 440927

End: 442861

Strand: Direct

Name: ykoU [H]

Synonym: Bind_0382

Alternate gene names: 182677378

Gene position: 440927-442861 (Clockwise)

Preceding gene: 182677377

Following gene: 182677379

Centisome position: 10.57

GC content: 55.14

Gene sequence:

>1935_bases
ATGGCCGCTCTCGACACCCATCACCAGAAACGCGCGAAAGCATCACCCTTTTTAGATTCTCTCGCCATCCAGGGAGCAAA
AAAGGCCTCGCTGCCCGAATTTGTCGAGCCGATGCTGGCGAGCCTCTTAACGGCCCCACCCGCTGGAAAAGACTGGATTC
ATGAAATCAAATTCGACGGCTATCGCCTGCAGGCACGGATCGAAGCCGGTCGCGTCAAACTGTTGACCCGTCGTGGACTG
GATTGGACGCAGAAGTTCGGCCCAAAGATCGTGACTATCTTGCAGGCTTTGCCCGTGGAAACAGCTTTGATCGATGGCGA
ACTCGTGGTCGAGAACAGCTCCGGTGTCTCTGACTTTTCCGCGCTGCAGGCGGCCTTAAGTGAGGGGCAGTTTGATCGCT
TTCTCTTTTATGCTTTCGATCTGCTTTATCTCGATGGCTATGATCTGTGTGATTTGTCACTAAGCAAACGCCGGGACATG
CTCGAACATCTTGTCGGCAGCGACGCTGGTCCTCTCCGCATGAGCCAACGCTTTGAAGAAGAGGGCCGTCTGGTCCTGCA
GCATGCGTGTCGTCTCGGTCTCGAAGGCATCATCTCCAAGCGGAAGGAGAGTGTCTACCGATCCGGACGTGGCAAGGATT
GGATCAAATCGAAATGTTCGGCGCGTCAGGAATTCGTCATCGCCGGTTATGTGCCCTCTTCCACGTCCCGCAAGGCGATC
GGTTCGCTTGTGCTCGGCGTCTATGACGGAGACAGGCTTGATTATGTTGGCCGGGTCGGAACAGGTTTTACAATGGCCAT
CGCCGAGGATCTCTTTCGCAAGCTCGAGGCGATGCAGATCGACAAAAGGCCTTTCGCGAAACCTCTGGCTTCGGCCGAAG
CGCGTCATGTGCGCTATGTCCATCCCGAGCTTGTTGCGGAGATTGAATTTTCTGGATGGACCGCTGACGAACGTCTTCGG
CACGCTTCGTTTCAAGGGCTGAGGGAAGACAAAGATGCTCGGGAGGTTGTGCGCGAGGGGTCAAGGAGCCTAACCTTCAC
GGCAAAGCCACAACGACGCCGGGCCGTATTCACGCATTCAGACCGTATCTATTGGCCGGATGAGGGTGTGACCAAGGAAG
GCCTGGCGGATTATTATACGGAGGTCTGGCGGTTCATCGCTCCCTTTATCGTTGGGCGCCCTTTGGCTCTGCTTCGTTGT
CCGGATGGCATTACTGGACCGCAATTCTTTCAAAAACATGCCTGGAAGAAGGTCTATTCCCATCTGGTTCTGGTCAACGA
TCCTAAAGAGAAATCGGAGCCTCTCCTCGCACTTCATGATCTCGATGGTTTACTCGAGCTTGTGCAATCAGCTGTCCTGG
AGATTCATCCCTGGCAATCCACCCTTACTGACTGGGAACGGCCGGATCGAATCATCATGGATCTTGACCCTGGTGAAGGC
GTTCCATGGGAAATGGTTATCGCGGCCGCCGAGGAGATGCGTGATCGTTTACAGGAAGTTGGCCTCGCGGCCTTTGTCAA
AACATCGGGTGGCAAAGGCTTGCATGTCGTCTCGCCTCTAAAACCGCTGGCGACTTGGCCCATGGTGAAGACTTTCACCA
AAACCCTGGCTGATGCCATGGCCGCTGACAATCCTGACCGTTTCATCTCAACGAGCACCAAGAGCAAGCGGCAAGGCAAG
ATTTTCATCGATTATCTGCGCAATCAACGAGGGATGACCGCTGTTGCTGCTTATTCGACCAGGGCGCGGCCTGGAGCTTC
TGTGTCGGTGCCTTTGGACTGGAAGGAGTTGAGCCCAAATATCGGACCAGCCTATTTTACGGTGGAGAATACACCGATCC
GCTTGGCTGCACTCACGGGAGATCCATGGGCGGAGTTTCAGGCGGCTGCGGTTGCTCTCGAAGAGCCAAGATCGACGAGA
CGCCGACATGGCTAG

Upstream 100 bases:

>100_bases
TTGGTGGCGAAAAAAGCCGCCTCATCGTCTTCTGGCAAGTCGAAAGCGACGGCCCCAAAGGAAAAGGCCACGCCTCGTCG
ACGCAAGGCGAGTTAAGGGC

Downstream 100 bases:

>100_bases
TATCGAGAATTACAGATTCTCGATACGCCCCTTTTTAAACCGTATCCACCTCATTGAACCCGACTCGTCGCGGTTCAATG
AGGGTGGATACTAGAGCATT

Product: DNA ligase D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MAALDTHHQKRAKASPFLDSLAIQGAKKASLPEFVEPMLASLLTAPPAGKDWIHEIKFDGYRLQARIEAGRVKLLTRRGL
DWTQKFGPKIVTILQALPVETALIDGELVVENSSGVSDFSALQAALSEGQFDRFLFYAFDLLYLDGYDLCDLSLSKRRDM
LEHLVGSDAGPLRMSQRFEEEGRLVLQHACRLGLEGIISKRKESVYRSGRGKDWIKSKCSARQEFVIAGYVPSSTSRKAI
GSLVLGVYDGDRLDYVGRVGTGFTMAIAEDLFRKLEAMQIDKRPFAKPLASAEARHVRYVHPELVAEIEFSGWTADERLR
HASFQGLREDKDAREVVREGSRSLTFTAKPQRRRAVFTHSDRIYWPDEGVTKEGLADYYTEVWRFIAPFIVGRPLALLRC
PDGITGPQFFQKHAWKKVYSHLVLVNDPKEKSEPLLALHDLDGLLELVQSAVLEIHPWQSTLTDWERPDRIIMDLDPGEG
VPWEMVIAAAEEMRDRLQEVGLAAFVKTSGGKGLHVVSPLKPLATWPMVKTFTKTLADAMAADNPDRFISTSTKSKRQGK
IFIDYLRNQRGMTAVAAYSTRARPGASVSVPLDWKELSPNIGPAYFTVENTPIRLAALTGDPWAEFQAAAVALEEPRSTR
RRHG

Sequences:

>Translated_644_residues
MAALDTHHQKRAKASPFLDSLAIQGAKKASLPEFVEPMLASLLTAPPAGKDWIHEIKFDGYRLQARIEAGRVKLLTRRGL
DWTQKFGPKIVTILQALPVETALIDGELVVENSSGVSDFSALQAALSEGQFDRFLFYAFDLLYLDGYDLCDLSLSKRRDM
LEHLVGSDAGPLRMSQRFEEEGRLVLQHACRLGLEGIISKRKESVYRSGRGKDWIKSKCSARQEFVIAGYVPSSTSRKAI
GSLVLGVYDGDRLDYVGRVGTGFTMAIAEDLFRKLEAMQIDKRPFAKPLASAEARHVRYVHPELVAEIEFSGWTADERLR
HASFQGLREDKDAREVVREGSRSLTFTAKPQRRRAVFTHSDRIYWPDEGVTKEGLADYYTEVWRFIAPFIVGRPLALLRC
PDGITGPQFFQKHAWKKVYSHLVLVNDPKEKSEPLLALHDLDGLLELVQSAVLEIHPWQSTLTDWERPDRIIMDLDPGEG
VPWEMVIAAAEEMRDRLQEVGLAAFVKTSGGKGLHVVSPLKPLATWPMVKTFTKTLADAMAADNPDRFISTSTKSKRQGK
IFIDYLRNQRGMTAVAAYSTRARPGASVSVPLDWKELSPNIGPAYFTVENTPIRLAALTGDPWAEFQAAAVALEEPRSTR
RRHG
>Mature_643_residues
AALDTHHQKRAKASPFLDSLAIQGAKKASLPEFVEPMLASLLTAPPAGKDWIHEIKFDGYRLQARIEAGRVKLLTRRGLD
WTQKFGPKIVTILQALPVETALIDGELVVENSSGVSDFSALQAALSEGQFDRFLFYAFDLLYLDGYDLCDLSLSKRRDML
EHLVGSDAGPLRMSQRFEEEGRLVLQHACRLGLEGIISKRKESVYRSGRGKDWIKSKCSARQEFVIAGYVPSSTSRKAIG
SLVLGVYDGDRLDYVGRVGTGFTMAIAEDLFRKLEAMQIDKRPFAKPLASAEARHVRYVHPELVAEIEFSGWTADERLRH
ASFQGLREDKDAREVVREGSRSLTFTAKPQRRRAVFTHSDRIYWPDEGVTKEGLADYYTEVWRFIAPFIVGRPLALLRCP
DGITGPQFFQKHAWKKVYSHLVLVNDPKEKSEPLLALHDLDGLLELVQSAVLEIHPWQSTLTDWERPDRIIMDLDPGEGV
PWEMVIAAAEEMRDRLQEVGLAAFVKTSGGKGLHVVSPLKPLATWPMVKTFTKTLADAMAADNPDRFISTSTKSKRQGKI
FIDYLRNQRGMTAVAAYSTRARPGASVSVPLDWKELSPNIGPAYFTVENTPIRLAALTGDPWAEFQAAAVALEEPRSTRR
RHG

Specific function: Probably involved in the repair of DNA double-strand breaks by non-homologous-end joining (NHEJ) during spore germination [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Drosophila melanogaster, GI24641829, Length=224, Percent_Identity=26.7857142857143, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR014146
- InterPro:   IPR014145
- InterPro:   IPR014143 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 72115; Mature: 71984

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALDTHHQKRAKASPFLDSLAIQGAKKASLPEFVEPMLASLLTAPPAGKDWIHEIKFDG
CCCCCCHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
YRLQARIEAGRVKLLTRRGLDWTQKFGPKIVTILQALPVETALIDGELVVENSSGVSDFS
EEEEEEECCCCEEEEHHCCCCHHHHHCHHHHHHHHHCCCHHEEECCEEEEECCCCCHHHH
ALQAALSEGQFDRFLFYAFDLLYLDGYDLCDLSLSKRRDMLEHLVGSDAGPLRMSQRFEE
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
EGRLVLQHACRLGLEGIISKRKESVYRSGRGKDWIKSKCSARQEFVIAGYVPSSTSRKAI
HHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCHHHH
GSLVLGVYDGDRLDYVGRVGTGFTMAIAEDLFRKLEAMQIDKRPFAKPLASAEARHVRYV
HHHEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEE
HPELVAEIEFSGWTADERLRHASFQGLREDKDAREVVREGSRSLTFTAKPQRRRAVFTHS
CHHHHEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCHHCEEEEEC
DRIYWPDEGVTKEGLADYYTEVWRFIAPFIVGRPLALLRCPDGITGPQFFQKHAWKKVYS
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHH
HLVLVNDPKEKSEPLLALHDLDGLLELVQSAVLEIHPWQSTLTDWERPDRIIMDLDPGEG
CEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHEECCCHHHHCCCCCCCEEEEECCCCCC
VPWEMVIAAAEEMRDRLQEVGLAAFVKTSGGKGLHVVSPLKPLATWPMVKTFTKTLADAM
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
AADNPDRFISTSTKSKRQGKIFIDYLRNQRGMTAVAAYSTRARPGASVSVPLDWKELSPN
HCCCCCCEEECCCCCCCCCCEEEEHHHCCCCCEEEEEECCCCCCCCEEECCCCHHHCCCC
IGPAYFTVENTPIRLAALTGDPWAEFQAAAVALEEPRSTRRRHG
CCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure 
AALDTHHQKRAKASPFLDSLAIQGAKKASLPEFVEPMLASLLTAPPAGKDWIHEIKFDG
CCCCCHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
YRLQARIEAGRVKLLTRRGLDWTQKFGPKIVTILQALPVETALIDGELVVENSSGVSDFS
EEEEEEECCCCEEEEHHCCCCHHHHHCHHHHHHHHHCCCHHEEECCEEEEECCCCCHHHH
ALQAALSEGQFDRFLFYAFDLLYLDGYDLCDLSLSKRRDMLEHLVGSDAGPLRMSQRFEE
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
EGRLVLQHACRLGLEGIISKRKESVYRSGRGKDWIKSKCSARQEFVIAGYVPSSTSRKAI
HHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCHHHH
GSLVLGVYDGDRLDYVGRVGTGFTMAIAEDLFRKLEAMQIDKRPFAKPLASAEARHVRYV
HHHEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEE
HPELVAEIEFSGWTADERLRHASFQGLREDKDAREVVREGSRSLTFTAKPQRRRAVFTHS
CHHHHEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCHHCEEEEEC
DRIYWPDEGVTKEGLADYYTEVWRFIAPFIVGRPLALLRCPDGITGPQFFQKHAWKKVYS
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHH
HLVLVNDPKEKSEPLLALHDLDGLLELVQSAVLEIHPWQSTLTDWERPDRIIMDLDPGEG
CEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHEECCCHHHHCCCCCCCEEEEECCCCCC
VPWEMVIAAAEEMRDRLQEVGLAAFVKTSGGKGLHVVSPLKPLATWPMVKTFTKTLADAM
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
AADNPDRFISTSTKSKRQGKIFIDYLRNQRGMTAVAAYSTRARPGASVSVPLDWKELSPN
HCCCCCCEEECCCCCCCCCCEEEEHHHCCCCCEEEEEECCCCCCCCEEECCCCHHHCCCC
IGPAYFTVENTPIRLAALTGDPWAEFQAAAVALEEPRSTRRRHG
CCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]