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The map label for this gene is gph [H]

Identifier: 182677161

GI number: 182677161

Start: 189641

End: 190315

Strand: Direct

Name: gph [H]

Synonym: Bind_0160

Alternate gene names: 182677161

Gene position: 189641-190315 (Clockwise)

Preceding gene: 182677160

Following gene: 182677162

Centisome position: 4.55

GC content: 59.56

Gene sequence:

>675_bases
ATGGATATGCGGCCGGTCTTTCTCTTCGACCTTGATGGAACGCTTGTCGATAGTGTGTATCAGCATGTGCTCGCCTGGCA
GGAGGCCCTGGACATCGAAGGAATCCCTTTGTCCGTCTGGCGCATTCATCGCAAGATCGGCATGAGCGGCGGTCTGTTTA
CCCATCAATTGCTGAGGGAAACGAAGGTCGAAATCAGCCCGGAGCGGATCGACCGGCTGCGTCGCCTCCATGCGGAGGCT
TATCGGCACCATGCCGCGAAGATAAAGCCGCTGCCCGGTGCCCGCGAATTGCTGGATACTTTGACGCGGGAGCAAATTCC
CTGGGCGATCGCCACCAGCGGACGGATGGAGACCGCTGCCAAAAATCTCGAGGCGCTTGGCGTCGATCCGTCGAAATCGC
CGGTCGTCACACGCGATGACGTCAAATATGCCAAGCCCGATCCGGATCTGTTTCTGGCCGCCGCGGCAAGGCTTGATACG
CCGACGACCGCTTCGATTGTGGTGGGAGACAGTATCTGGGACATTCTCGCGGCGCGCCGTTGTCAGGCGCTTGGTGTCGG
CGTTCTCTCAGGAGGTTACGGCGCCGATGAACTGGAGCGAGCCGGAGCCTATCGGGTCTATGAAGATCCGGCCGATATTC
TTTTGCATCTCGATGAATTGGGCGGGCGCCGCTGA

Upstream 100 bases:

>100_bases
TCGAGGCGCTCGGCGTGCCGGTGCGCAGCCTGATCTCTTTTGAAGGGCATTAAACAGCTTCGGGAATGTCGCGCGCCAAT
CCAAACAATCGGAGGTCAGG

Downstream 100 bases:

>100_bases
TCGACGCTCCATAACGGAAAGTGTGCATCCTTTTGGGAGCGGAACTGGCTGAGCGTCCGATGTCTTAAACCATGAGGTGC
GGCGATCCATGAAATTCAGC

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MDMRPVFLFDLDGTLVDSVYQHVLAWQEALDIEGIPLSVWRIHRKIGMSGGLFTHQLLRETKVEISPERIDRLRRLHAEA
YRHHAAKIKPLPGARELLDTLTREQIPWAIATSGRMETAAKNLEALGVDPSKSPVVTRDDVKYAKPDPDLFLAAAARLDT
PTTASIVVGDSIWDILAARRCQALGVGVLSGGYGADELERAGAYRVYEDPADILLHLDELGGRR

Sequences:

>Translated_224_residues
MDMRPVFLFDLDGTLVDSVYQHVLAWQEALDIEGIPLSVWRIHRKIGMSGGLFTHQLLRETKVEISPERIDRLRRLHAEA
YRHHAAKIKPLPGARELLDTLTREQIPWAIATSGRMETAAKNLEALGVDPSKSPVVTRDDVKYAKPDPDLFLAAAARLDT
PTTASIVVGDSIWDILAARRCQALGVGVLSGGYGADELERAGAYRVYEDPADILLHLDELGGRR
>Mature_224_residues
MDMRPVFLFDLDGTLVDSVYQHVLAWQEALDIEGIPLSVWRIHRKIGMSGGLFTHQLLRETKVEISPERIDRLRRLHAEA
YRHHAAKIKPLPGARELLDTLTREQIPWAIATSGRMETAAKNLEALGVDPSKSPVVTRDDVKYAKPDPDLFLAAAARLDT
PTTASIVVGDSIWDILAARRCQALGVGVLSGGYGADELERAGAYRVYEDPADILLHLDELGGRR

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI87082080, Length=185, Percent_Identity=34.5945945945946, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24839; Mature: 24839

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMRPVFLFDLDGTLVDSVYQHVLAWQEALDIEGIPLSVWRIHRKIGMSGGLFTHQLLRE
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
TKVEISPERIDRLRRLHAEAYRHHAAKIKPLPGARELLDTLTREQIPWAIATSGRMETAA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHH
KNLEALGVDPSKSPVVTRDDVKYAKPDPDLFLAAAARLDTPTTASIVVGDSIWDILAARR
HHHHHCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECHHHHHHHHHHH
CQALGVGVLSGGYGADELERAGAYRVYEDPADILLHLDELGGRR
HHHHCCEEECCCCCHHHHHHCCCCEEECCHHHHHEEHHHHCCCC
>Mature Secondary Structure
MDMRPVFLFDLDGTLVDSVYQHVLAWQEALDIEGIPLSVWRIHRKIGMSGGLFTHQLLRE
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
TKVEISPERIDRLRRLHAEAYRHHAAKIKPLPGARELLDTLTREQIPWAIATSGRMETAA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHH
KNLEALGVDPSKSPVVTRDDVKYAKPDPDLFLAAAARLDTPTTASIVVGDSIWDILAARR
HHHHHCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECHHHHHHHHHHH
CQALGVGVLSGGYGADELERAGAYRVYEDPADILLHLDELGGRR
HHHHCCEEECCCCCHHHHHHCCCCEEECCHHHHHEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]