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The map label for this gene is priA [H]

Identifier: 182682659

GI number: 182682659

Start: 2427073

End: 2429274

Strand: Direct

Name: priA [H]

Synonym: XfasM23_2149

Alternate gene names: 182682659

Gene position: 2427073-2429274 (Clockwise)

Preceding gene: 182682650

Following gene: 182682667

Centisome position: 95.72

GC content: 54.18

Gene sequence:

>2202_bases
ATGCCCCCTACGATCCTACGTGTGGCGTTGCCAATCCCTTTACCGCAATGCTTCGACTATCTGCCGCCAAGCGGCATACC
ACTCCCAGATTCAACCTTAGGCTGCCGCGTACGTGTGCCATTCGGGAAACGTGAGCTGATCGGTGTAGTCGTCGAACTTC
CATCAGCCGAAATGTCAGTACCATTACGCGAGGCATTCGCCTGGATTGACCGTATACCAATTCTAGATGGCGAGCTGGCA
CAATCGTTACAGTGGCTGTCACACTACACGCATACCCCACTAGGTGAGATATTAGCTACGGCTCTTCCCGGGCTGTTACG
TCATGGTAAACCCTTACCAGAGACCTGGACCTGCTGGGCTTGGCGTCTAACCGAAACCGGGCGCACTGCCGCTCCCAAGC
TGCGTCGCAACAGCCGCTCAGCACAAATGGCTGAATTACTCACCGCCAGTGATCAAACGGAAGATGCACTTAACACTCGG
ATGAATCGCTGGCGCGCAGTTGCACACAACCTAACCAAGCGTGGTTATGTCGAACGCATCAAGAAACCTACAACGGTACC
TGTAATCATCCAGACCACAAGCAACACCGTACCTGTACTCAACACTGAACAACAGAAAGCAGTTGAGACACTAAACGCTA
ACGTAGGCTTCAAAACTTATCTTCTAGATGGAGTCACCGGCAGCGGTAAAACAGAAGTCTACCTACAAGCCATCGCTACC
TGCTTAGCTGCGAGTAAACAAGCATTGGTCTTAGTCCCTGAGATCGGCTTGACACCTCAACTTCTAACCAACTTCCATGC
CCGCCTCGGAATACCCGTGCATGCCCTGCACTCTAAATTGGCAGACAGCGAACGCGCCCGCGTGTGGGCGACGGCACGAT
GTGGCGAGGCACGACTGGTACTAGGAACCCGCTCAGCGGTGTTCACCCCCCTACCTAATGCCGGACTAATTATAGTAGAT
GAGGAACACGACACGAGTTACAAGCAGCAAGACGGGATACGTTACAACGCGCGCGATTTCGCATTGGTGCGCGGCAAGGC
ACTGAACGTACCGGTCATCCTCGGGAGTGCCACTCCGTCACTGGAATCACTGTACAACGCGCATAACGGACGCTACAAGC
ACCTCCGCCTCAGCTACCGTGCCGGTCAAGCGTATGCCCCTCAGGTGCGCGTACTAGACGTGCGCAAAAGACCACTGAAA
GACGGCCTATCACCCGAGGTATTAACAGGAATTAACGCAACTTTAGCGCGTGATGAGCAGGTACTCGTATTCAAGAACCG
GTGCGGCTATTCACCAGTGTTGCTATGTCACGACTGCGGCTGGACCGCTACCTGTCACCAGTGCAGTACATCAGTGCACC
ACACACCAATGACTGTATACAGCGGCGGAACCTATTTACAGTGCCACCACTGCGGCGCACGCCAGCCAATGCCACTATCA
TGCCCAGACTGCGGCAGTACAGCGCTACAGCCACAGGGCATAGGCACCGAACGCCTAGAAGCTCGCCTGATTGAGGCTTT
CCCTAAACATCCTGTGGTGCGTATTGATCGCAACACAACCAAGCACCGCGACGGACTAAAAACCCAATTCACCAAACTCG
GCAATGCCGCTGGCATTTTAGTAGGCACACAGATTCTAGCCAAAGGGCATCACCTACCACGCTTAACGATGGTCGTGGTC
GTTGGAATCGACGAAGGCCTATTCTCAGCCGACTTCCGTGCCACTGAAAAGCTCTCCCAGCAACTGATCCAAGTTGCTGG
CCGCGCTGGCCGTGCTAAACACGCCGGAGAAGTCTGGCTACAAACTCACCACCCTGATCACCCTCTGTTGCAAACACTAA
TCAACGGCGGCTACCACACCTTTGCTAATACGGAGCTACAACAACGCCAAGCCGCTTGGTTACCACCATTCGCACACTTG
GCACTCCTGCGTGCTGAAGCGAAAGACACAACCTCAACACAACAGTTTCTCACCACGATACGCAAACACATGCCAACCAA
CAATGCAGTCGAATGCTACGGCCCAATGTCGGCTCCCATGCCGCGCCGCGCCGGCTACGAGCGCTACCAATTATTGCTGT
CCAGCTCCCAACGTAACGCACTACACCGCACACTCAATGTCTTGATACCAGCAATTCATGCACTGCCAGAAGCACGCCGA
GTCCGCTGGTCGTTAGACGTGGATCCAGTTGATCTTTACTAA

Upstream 100 bases:

>100_bases
AAATAGAACGATAAGTTACCTTTTGTTTATGAGAACAACCACGAGAATAGAGATCAAACTACACACTCTGTTATCGGCAT
CCGATCACTAAACCTTCTCC

Downstream 100 bases:

>100_bases
GGTAATACTTCCAAAAATTTAACAAAAAATAAAATAATAAACCGGCATCACCAAAAATAAAAAATTCCAAAAAAATTCTC
TGGTAACCAGCAACGATGGT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MPPTILRVALPIPLPQCFDYLPPSGIPLPDSTLGCRVRVPFGKRELIGVVVELPSAEMSVPLREAFAWIDRIPILDGELA
QSLQWLSHYTHTPLGEILATALPGLLRHGKPLPETWTCWAWRLTETGRTAAPKLRRNSRSAQMAELLTASDQTEDALNTR
MNRWRAVAHNLTKRGYVERIKKPTTVPVIIQTTSNTVPVLNTEQQKAVETLNANVGFKTYLLDGVTGSGKTEVYLQAIAT
CLAASKQALVLVPEIGLTPQLLTNFHARLGIPVHALHSKLADSERARVWATARCGEARLVLGTRSAVFTPLPNAGLIIVD
EEHDTSYKQQDGIRYNARDFALVRGKALNVPVILGSATPSLESLYNAHNGRYKHLRLSYRAGQAYAPQVRVLDVRKRPLK
DGLSPEVLTGINATLARDEQVLVFKNRCGYSPVLLCHDCGWTATCHQCSTSVHHTPMTVYSGGTYLQCHHCGARQPMPLS
CPDCGSTALQPQGIGTERLEARLIEAFPKHPVVRIDRNTTKHRDGLKTQFTKLGNAAGILVGTQILAKGHHLPRLTMVVV
VGIDEGLFSADFRATEKLSQQLIQVAGRAGRAKHAGEVWLQTHHPDHPLLQTLINGGYHTFANTELQQRQAAWLPPFAHL
ALLRAEAKDTTSTQQFLTTIRKHMPTNNAVECYGPMSAPMPRRAGYERYQLLLSSSQRNALHRTLNVLIPAIHALPEARR
VRWSLDVDPVDLY

Sequences:

>Translated_733_residues
MPPTILRVALPIPLPQCFDYLPPSGIPLPDSTLGCRVRVPFGKRELIGVVVELPSAEMSVPLREAFAWIDRIPILDGELA
QSLQWLSHYTHTPLGEILATALPGLLRHGKPLPETWTCWAWRLTETGRTAAPKLRRNSRSAQMAELLTASDQTEDALNTR
MNRWRAVAHNLTKRGYVERIKKPTTVPVIIQTTSNTVPVLNTEQQKAVETLNANVGFKTYLLDGVTGSGKTEVYLQAIAT
CLAASKQALVLVPEIGLTPQLLTNFHARLGIPVHALHSKLADSERARVWATARCGEARLVLGTRSAVFTPLPNAGLIIVD
EEHDTSYKQQDGIRYNARDFALVRGKALNVPVILGSATPSLESLYNAHNGRYKHLRLSYRAGQAYAPQVRVLDVRKRPLK
DGLSPEVLTGINATLARDEQVLVFKNRCGYSPVLLCHDCGWTATCHQCSTSVHHTPMTVYSGGTYLQCHHCGARQPMPLS
CPDCGSTALQPQGIGTERLEARLIEAFPKHPVVRIDRNTTKHRDGLKTQFTKLGNAAGILVGTQILAKGHHLPRLTMVVV
VGIDEGLFSADFRATEKLSQQLIQVAGRAGRAKHAGEVWLQTHHPDHPLLQTLINGGYHTFANTELQQRQAAWLPPFAHL
ALLRAEAKDTTSTQQFLTTIRKHMPTNNAVECYGPMSAPMPRRAGYERYQLLLSSSQRNALHRTLNVLIPAIHALPEARR
VRWSLDVDPVDLY
>Mature_732_residues
PPTILRVALPIPLPQCFDYLPPSGIPLPDSTLGCRVRVPFGKRELIGVVVELPSAEMSVPLREAFAWIDRIPILDGELAQ
SLQWLSHYTHTPLGEILATALPGLLRHGKPLPETWTCWAWRLTETGRTAAPKLRRNSRSAQMAELLTASDQTEDALNTRM
NRWRAVAHNLTKRGYVERIKKPTTVPVIIQTTSNTVPVLNTEQQKAVETLNANVGFKTYLLDGVTGSGKTEVYLQAIATC
LAASKQALVLVPEIGLTPQLLTNFHARLGIPVHALHSKLADSERARVWATARCGEARLVLGTRSAVFTPLPNAGLIIVDE
EHDTSYKQQDGIRYNARDFALVRGKALNVPVILGSATPSLESLYNAHNGRYKHLRLSYRAGQAYAPQVRVLDVRKRPLKD
GLSPEVLTGINATLARDEQVLVFKNRCGYSPVLLCHDCGWTATCHQCSTSVHHTPMTVYSGGTYLQCHHCGARQPMPLSC
PDCGSTALQPQGIGTERLEARLIEAFPKHPVVRIDRNTTKHRDGLKTQFTKLGNAAGILVGTQILAKGHHLPRLTMVVVV
GIDEGLFSADFRATEKLSQQLIQVAGRAGRAKHAGEVWLQTHHPDHPLLQTLINGGYHTFANTELQQRQAAWLPPFAHLA
LLRAEAKDTTSTQQFLTTIRKHMPTNNAVECYGPMSAPMPRRAGYERYQLLLSSSQRNALHRTLNVLIPAIHALPEARRV
RWSLDVDPVDLY

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=743, Percent_Identity=43.3378196500673, Blast_Score=578, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81040; Mature: 80909

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPTILRVALPIPLPQCFDYLPPSGIPLPDSTLGCRVRVPFGKRELIGVVVELPSAEMSV
CCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCC
PLREAFAWIDRIPILDGELAQSLQWLSHYTHTPLGEILATALPGLLRHGKPLPETWTCWA
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
WRLTETGRTAAPKLRRNSRSAQMAELLTASDQTEDALNTRMNRWRAVAHNLTKRGYVERI
EEEECCCCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKPTTVPVIIQTTSNTVPVLNTEQQKAVETLNANVGFKTYLLDGVTGSGKTEVYLQAIAT
CCCCCEEEEEEECCCCCCEECCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHH
CLAASKQALVLVPEIGLTPQLLTNFHARLGIPVHALHSKLADSERARVWATARCGEARLV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCEEEE
LGTRSAVFTPLPNAGLIIVDEEHDTSYKQQDGIRYNARDFALVRGKALNVPVILGSATPS
EECCCEEECCCCCCCEEEEECCCCCCCHHHCCCEECCCCEEEECCCCCCCCEEECCCCCH
LESLYNAHNGRYKHLRLSYRAGQAYAPQVRVLDVRKRPLKDGLSPEVLTGINATLARDEQ
HHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEECCCCHHHCCCCHHHHHCCCCEECCCCE
VLVFKNRCGYSPVLLCHDCGWTATCHQCSTSVHHTPMTVYSGGTYLQCHHCGARQPMPLS
EEEEECCCCCCCEEEEECCCCCCHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCCCCCC
CPDCGSTALQPQGIGTERLEARLIEAFPKHPVVRIDRNTTKHRDGLKTQFTKLGNAAGIL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHCCHHHHHHHCCCCCCHH
VGTQILAKGHHLPRLTMVVVVGIDEGLFSADFRATEKLSQQLIQVAGRAGRAKHAGEVWL
HHHHHHHCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
QTHHPDHPLLQTLINGGYHTFANTELQQRQAAWLPPFAHLALLRAEAKDTTSTQQFLTTI
ECCCCCCHHHHHHHCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHH
RKHMPTNNAVECYGPMSAPMPRRAGYERYQLLLSSSQRNALHRTLNVLIPAIHALPEARR
HHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
VRWSLDVDPVDLY
EEEECCCCCCCCC
>Mature Secondary Structure 
PPTILRVALPIPLPQCFDYLPPSGIPLPDSTLGCRVRVPFGKRELIGVVVELPSAEMSV
CCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCC
PLREAFAWIDRIPILDGELAQSLQWLSHYTHTPLGEILATALPGLLRHGKPLPETWTCWA
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
WRLTETGRTAAPKLRRNSRSAQMAELLTASDQTEDALNTRMNRWRAVAHNLTKRGYVERI
EEEECCCCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKPTTVPVIIQTTSNTVPVLNTEQQKAVETLNANVGFKTYLLDGVTGSGKTEVYLQAIAT
CCCCCEEEEEEECCCCCCEECCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHH
CLAASKQALVLVPEIGLTPQLLTNFHARLGIPVHALHSKLADSERARVWATARCGEARLV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCEEEE
LGTRSAVFTPLPNAGLIIVDEEHDTSYKQQDGIRYNARDFALVRGKALNVPVILGSATPS
EECCCEEECCCCCCCEEEEECCCCCCCHHHCCCEECCCCEEEECCCCCCCCEEECCCCCH
LESLYNAHNGRYKHLRLSYRAGQAYAPQVRVLDVRKRPLKDGLSPEVLTGINATLARDEQ
HHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEECCCCHHHCCCCHHHHHCCCCEECCCCE
VLVFKNRCGYSPVLLCHDCGWTATCHQCSTSVHHTPMTVYSGGTYLQCHHCGARQPMPLS
EEEEECCCCCCCEEEEECCCCCCHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCCCCCC
CPDCGSTALQPQGIGTERLEARLIEAFPKHPVVRIDRNTTKHRDGLKTQFTKLGNAAGIL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHCCHHHHHHHCCCCCCHH
VGTQILAKGHHLPRLTMVVVVGIDEGLFSADFRATEKLSQQLIQVAGRAGRAKHAGEVWL
HHHHHHHCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
QTHHPDHPLLQTLINGGYHTFANTELQQRQAAWLPPFAHLALLRAEAKDTTSTQQFLTTI
ECCCCCCHHHHHHHCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHH
RKHMPTNNAVECYGPMSAPMPRRAGYERYQLLLSSSQRNALHRTLNVLIPAIHALPEARR
HHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
VRWSLDVDPVDLY
EEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]