Click here to switch to the map view.

The map label for this gene is glcD [H]

Identifier: 182682318

GI number: 182682318

Start: 2009454

End: 2010839

Strand: Direct

Name: glcD [H]

Synonym: XfasM23_1801

Alternate gene names: 182682318

Gene position: 2009454-2010839 (Clockwise)

Preceding gene: 182682309

Following gene: 182682319

Centisome position: 79.25

GC content: 54.83

Gene sequence:

>1386_bases
ATGACCACTCAATTGCCCGATGTACTGATCACTACTCTGTCCACTCTCCTGGGCCCGGAAGGCTGGCGCCTTGATGCCAT
CAGTCGCCGCCAGTACAGCGAAGACGACTCGCAGTGCTTTGCCTGTGCCGATGCAGTGGCGCTGCCACGTGACCTCAACG
AGGTAGTGGCAATCGTACGTGCCTGCCATCAACATCGCGTGCCAGTGATCCCACGAGGTACCGGGACTGGCACCGTGGGT
GGTGCAGTGCCGCTCCATGGCGGCATCGTTCTGTCATTGGCACGTATGAACCGTATCGTCGCACTGCTGCCAAATGATCG
CTGCGCAGTGGTACAGCCTGGGGTGATCAACAATGACTTACAACAAGCACTGGCACCACATGGGCTTTTTTGGCCACCAG
ATCCGTCCAGTACAGCGATGTGCAGTATCGGCGGCAATCTTGCAACGAATGCCGGTGGCCCGCGTACGGTGAAGTACGGT
GCCACACGAGATAACGTGCTTGGCGTCGTCGCAGTGACCGGTGTCGGCGATGTAATCCGCTGTGGCGGTGCTTATACGAA
GAACGCCACCGGTTACGATCTGGCACATCTGTTGATTGGCAGTGAAGGCACATTAGCAATCATCGTCGAGGCCACGTTAA
AGCTGGCACCACAACCACAAGCTATCGCTGGACTGCGTGCGTTCTATCAAGATACGGCCGCTGCGGCTGCCGCAGTGTCA
CGACTGATGTCACAACCGGCAATACCAGCAACGCTGGAGTTCATGGACCACAAAGCAATCGAGCTGCTGTATCGCAATGG
CAGCAATATTCCTATGGCCGGCGCGATGCTGCTGATTGAAGGAGACGGCAATCACGACACGCTTCCCTATGTATTACAAG
CACTGGCGCAGGCCGCTTCGGGTGAGGGGCTGATAGACCTTGACATGGCCATGGATGGAGCCGCACGCGATCAACTATGG
GCAGCACGCAAGTCACTATCGCCAATGCTTCGCACGATTAAACCTGGAAAACTAAACGAAGACGTTGTTGTTCCCGTCTC
GCGTATTCCTGATTTGGTTGCTGGCATAGAAGTATTGGCTACCGAGAGCGGATTACCCATTGTCACCTTTGGGCACGCCG
GTAACGGAAATCTACATGTGAATATCATGTATGACGATCAGGATGCGGAAGACATTATGCGGGCGCAGGCAACGCTACCA
AAGCTATTTAAGTTAGTTTTGACTCTTGGAGGCACCCTGTCAGGTGAGCACGGGATTGGCGTAGCCAAACGTGACTTCAT
GACACAAGCATTCGACACACCAACGCTAGCAGCAATGAGTGCGATCAAGGCCGCATTCGATCCAGACGGTATTCTCAATC
CAGGTAAAGTGCTACCCAGAGTCTAA

Upstream 100 bases:

>100_bases
GCGAAAATGATTCCACAAAACAGACTCCAATGAATCAGCCTTGCTGCTGGCATGAAATGGATACTCTTTTTTCTAACCAC
GATGTGTTGCTCTGTTCCTG

Downstream 100 bases:

>100_bases
GAAACATATTACCCCCAGAACTCTCCTGAGACGTTTAAACAGGTGAAGACTGAGGCGGTAATGCTTGAGTCTAACGAAAT
ACCACATCACGCATGGAGAT

Product: FAD linked oxidase domain-containing protein

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTTQLPDVLITTLSTLLGPEGWRLDAISRRQYSEDDSQCFACADAVALPRDLNEVVAIVRACHQHRVPVIPRGTGTGTVG
GAVPLHGGIVLSLARMNRIVALLPNDRCAVVQPGVINNDLQQALAPHGLFWPPDPSSTAMCSIGGNLATNAGGPRTVKYG
ATRDNVLGVVAVTGVGDVIRCGGAYTKNATGYDLAHLLIGSEGTLAIIVEATLKLAPQPQAIAGLRAFYQDTAAAAAAVS
RLMSQPAIPATLEFMDHKAIELLYRNGSNIPMAGAMLLIEGDGNHDTLPYVLQALAQAASGEGLIDLDMAMDGAARDQLW
AARKSLSPMLRTIKPGKLNEDVVVPVSRIPDLVAGIEVLATESGLPIVTFGHAGNGNLHVNIMYDDQDAEDIMRAQATLP
KLFKLVLTLGGTLSGEHGIGVAKRDFMTQAFDTPTLAAMSAIKAAFDPDGILNPGKVLPRV

Sequences:

>Translated_461_residues
MTTQLPDVLITTLSTLLGPEGWRLDAISRRQYSEDDSQCFACADAVALPRDLNEVVAIVRACHQHRVPVIPRGTGTGTVG
GAVPLHGGIVLSLARMNRIVALLPNDRCAVVQPGVINNDLQQALAPHGLFWPPDPSSTAMCSIGGNLATNAGGPRTVKYG
ATRDNVLGVVAVTGVGDVIRCGGAYTKNATGYDLAHLLIGSEGTLAIIVEATLKLAPQPQAIAGLRAFYQDTAAAAAAVS
RLMSQPAIPATLEFMDHKAIELLYRNGSNIPMAGAMLLIEGDGNHDTLPYVLQALAQAASGEGLIDLDMAMDGAARDQLW
AARKSLSPMLRTIKPGKLNEDVVVPVSRIPDLVAGIEVLATESGLPIVTFGHAGNGNLHVNIMYDDQDAEDIMRAQATLP
KLFKLVLTLGGTLSGEHGIGVAKRDFMTQAFDTPTLAAMSAIKAAFDPDGILNPGKVLPRV
>Mature_460_residues
TTQLPDVLITTLSTLLGPEGWRLDAISRRQYSEDDSQCFACADAVALPRDLNEVVAIVRACHQHRVPVIPRGTGTGTVGG
AVPLHGGIVLSLARMNRIVALLPNDRCAVVQPGVINNDLQQALAPHGLFWPPDPSSTAMCSIGGNLATNAGGPRTVKYGA
TRDNVLGVVAVTGVGDVIRCGGAYTKNATGYDLAHLLIGSEGTLAIIVEATLKLAPQPQAIAGLRAFYQDTAAAAAAVSR
LMSQPAIPATLEFMDHKAIELLYRNGSNIPMAGAMLLIEGDGNHDTLPYVLQALAQAASGEGLIDLDMAMDGAARDQLWA
ARKSLSPMLRTIKPGKLNEDVVVPVSRIPDLVAGIEVLATESGLPIVTFGHAGNGNLHVNIMYDDQDAEDIMRAQATLPK
LFKLVLTLGGTLSGEHGIGVAKRDFMTQAFDTPTLAAMSAIKAAFDPDGILNPGKVLPRV

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=459, Percent_Identity=34.8583877995643, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI37595754, Length=482, Percent_Identity=33.8174273858921, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI119964728, Length=433, Percent_Identity=33.4872979214781, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI4501993, Length=458, Percent_Identity=23.5807860262009, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1789351, Length=417, Percent_Identity=38.8489208633094, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1787977, Length=544, Percent_Identity=29.2279411764706, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI48994907, Length=437, Percent_Identity=23.3409610983982, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994914, Length=139, Percent_Identity=33.0935251798561, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71992373, Length=465, Percent_Identity=31.3978494623656, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI71992381, Length=465, Percent_Identity=29.8924731182796, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI17534361, Length=462, Percent_Identity=30.0865800865801, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI71992392, Length=382, Percent_Identity=30.6282722513089, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17556096, Length=218, Percent_Identity=24.7706422018349, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320023, Length=450, Percent_Identity=29.1111111111111, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6320764, Length=440, Percent_Identity=30.4545454545455, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6320027, Length=433, Percent_Identity=29.0993071593533, Blast_Score=169, Evalue=9e-43,
Organism=Drosophila melanogaster, GI18921117, Length=429, Percent_Identity=32.4009324009324, Blast_Score=204, Evalue=9e-53,
Organism=Drosophila melanogaster, GI24639277, Length=429, Percent_Identity=32.4009324009324, Blast_Score=204, Evalue=9e-53,
Organism=Drosophila melanogaster, GI24639275, Length=429, Percent_Identity=32.4009324009324, Blast_Score=204, Evalue=9e-53,
Organism=Drosophila melanogaster, GI24653753, Length=225, Percent_Identity=32, Blast_Score=95, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.1.28

Molecular weight: Translated: 48399; Mature: 48268

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQLPDVLITTLSTLLGPEGWRLDAISRRQYSEDDSQCFACADAVALPRDLNEVVAIVR
CCCCCHHHHHHHHHHHHCCCCCEECHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
ACHQHRVPVIPRGTGTGTVGGAVPLHGGIVLSLARMNRIVALLPNDRCAVVQPGVINNDL
HHHHCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCEEEECCCCCCHHH
QQALAPHGLFWPPDPSSTAMCSIGGNLATNAGGPRTVKYGATRDNVLGVVAVTGVGDVIR
HHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCHHHH
CGGAYTKNATGYDLAHLLIGSEGTLAIIVEATLKLAPQPQAIAGLRAFYQDTAAAAAAVS
CCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RLMSQPAIPATLEFMDHKAIELLYRNGSNIPMAGAMLLIEGDGNHDTLPYVLQALAQAAS
HHHHCCCCCHHHHHHHHHHHHHHEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCC
GEGLIDLDMAMDGAARDQLWAARKSLSPMLRTIKPGKLNEDVVVPVSRIPDLVAGIEVLA
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCHHHHHHHHEE
TESGLPIVTFGHAGNGNLHVNIMYDDQDAEDIMRAQATLPKLFKLVLTLGGTLSGEHGIG
ECCCCCEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VAKRDFMTQAFDTPTLAAMSAIKAAFDPDGILNPGKVLPRV
CHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
TTQLPDVLITTLSTLLGPEGWRLDAISRRQYSEDDSQCFACADAVALPRDLNEVVAIVR
CCCCHHHHHHHHHHHHCCCCCEECHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
ACHQHRVPVIPRGTGTGTVGGAVPLHGGIVLSLARMNRIVALLPNDRCAVVQPGVINNDL
HHHHCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCEEEECCCCCCHHH
QQALAPHGLFWPPDPSSTAMCSIGGNLATNAGGPRTVKYGATRDNVLGVVAVTGVGDVIR
HHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCHHHH
CGGAYTKNATGYDLAHLLIGSEGTLAIIVEATLKLAPQPQAIAGLRAFYQDTAAAAAAVS
CCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RLMSQPAIPATLEFMDHKAIELLYRNGSNIPMAGAMLLIEGDGNHDTLPYVLQALAQAAS
HHHHCCCCCHHHHHHHHHHHHHHEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCC
GEGLIDLDMAMDGAARDQLWAARKSLSPMLRTIKPGKLNEDVVVPVSRIPDLVAGIEVLA
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCHHHHHHHHEE
TESGLPIVTFGHAGNGNLHVNIMYDDQDAEDIMRAQATLPKLFKLVLTLGGTLSGEHGIG
ECCCCCEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VAKRDFMTQAFDTPTLAAMSAIKAAFDPDGILNPGKVLPRV
CHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]