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The map label for this gene is oprF [H]

Identifier: 182682319

GI number: 182682319

Start: 2012763

End: 2013890

Strand: Direct

Name: oprF [H]

Synonym: XfasM23_1802

Alternate gene names: 182682319

Gene position: 2012763-2013890 (Clockwise)

Preceding gene: 182682318

Following gene: 182682322

Centisome position: 79.38

GC content: 51.42

Gene sequence:

>1128_bases
ATGAAAAAGAAAATCCTTACTGCTGCGCTGCTGGGTGGTATAGCTATCATCCAAGTTGCTTCTGCACAGGAATTCGATGA
CCGGTGGTATCTCGCTGGTTCCACCGGGTTTAACTTCCAAGATAAGCGTCGTCTTACTAACGATGCTCCTTTTTTCGCTC
TTGGAATTGGTAAGTTCATCAATCCAGTTTGGTCGATCGACGGCACACTGAACTATCAGAACCCGCAGTTCAAAAGAAAC
AAAGATCTAAACTGGAGCCAATATGGCTTCTCTGTCGATTTTCGTCGCCATTTCATCCAGGATAACCGCGGCTGGAATCC
TTACTTATTGTTAGGAGCGGGGTACCAACGTTCAGAGGAGGAATTTGATAACACCCCAAACGTAAACTCCCCTGGCGAAC
GTAAGAAAGGCGGCTTTGCTGCTAAGGTAGGCGCTGGCTTGCAGACAACATTCAGAAGCCGTGTCGCCGTCCGTGCTGAA
GTAGCTTATCGTGGTGACCATGATAAGGATAGCGTGCGTCAGCCGGTGGCTGGTGTTGCGGCACCTTCCAGCAAGAAATG
GTTTGGTGATACCTTGGCTTCGATTGGTGTCGTGATCCCATTTGGTCCAGCCGCTTCCTCACCAACGCCACCACCTGCTC
CGCCTGCTCCAACACCGCCGCCGCCACCGCCTGCTCCAAGCTGTGCTGAATTGGATAGCGATGGAGATGGAGTCAACGAC
TGTGACGACAAGTGCCCGAACTCACAACCAGGCCAGACGATCGGACCAGATGGATGTCCGGTGCCAGTGTCGATTGACTT
AAAAGGCGTAAACTTCGATTTTGACAAGTCTAAGCTTCGTCCTGACGCCGTTGCGGTTCTTAAGGAGGCTACCGAAATCT
TGAGACGCTACCCTGACTTGCACGTAGAGGTGGCTGGCCATACCGATTCGACAGGTCCGGCTGCTTATAACAAAAAGCTA
TCGGAACGTCGCGCTAAAGTCGTGTATGACTACCTGCGTAACAATGGTATCGACGCATCTCGCCTGATTGGGCCAGTCGG
ATATGGGGAGACCCATCCAATTGATACAAACAAAACCCCAGCAGGTCGTGCGAAGAACCGCCGTACTGAGTTGAACGTTC
AGAATTGA

Upstream 100 bases:

>100_bases
CGCCAATGGTGTGTGGGACTGCTGTCTGGATGTGACAAGCATCATGTACTAACACAACATGCCCTGCAGTTTTTTATATC
CCTTGACTCAAGGAGCTGTT

Downstream 100 bases:

>100_bases
TCTGAATTGTAATGTGCTCCATAGCAAAGCCTGGCTTATGCCAGGCTTTGTTTTAGAAAGAAAAAAACGCTGTAACGCAT
CTTCTATACGGGAATCTTTC

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKKKILTAALLGGIAIIQVASAQEFDDRWYLAGSTGFNFQDKRRLTNDAPFFALGIGKFINPVWSIDGTLNYQNPQFKRN
KDLNWSQYGFSVDFRRHFIQDNRGWNPYLLLGAGYQRSEEEFDNTPNVNSPGERKKGGFAAKVGAGLQTTFRSRVAVRAE
VAYRGDHDKDSVRQPVAGVAAPSSKKWFGDTLASIGVVIPFGPAASSPTPPPAPPAPTPPPPPPAPSCAELDSDGDGVND
CDDKCPNSQPGQTIGPDGCPVPVSIDLKGVNFDFDKSKLRPDAVAVLKEATEILRRYPDLHVEVAGHTDSTGPAAYNKKL
SERRAKVVYDYLRNNGIDASRLIGPVGYGETHPIDTNKTPAGRAKNRRTELNVQN

Sequences:

>Translated_375_residues
MKKKILTAALLGGIAIIQVASAQEFDDRWYLAGSTGFNFQDKRRLTNDAPFFALGIGKFINPVWSIDGTLNYQNPQFKRN
KDLNWSQYGFSVDFRRHFIQDNRGWNPYLLLGAGYQRSEEEFDNTPNVNSPGERKKGGFAAKVGAGLQTTFRSRVAVRAE
VAYRGDHDKDSVRQPVAGVAAPSSKKWFGDTLASIGVVIPFGPAASSPTPPPAPPAPTPPPPPPAPSCAELDSDGDGVND
CDDKCPNSQPGQTIGPDGCPVPVSIDLKGVNFDFDKSKLRPDAVAVLKEATEILRRYPDLHVEVAGHTDSTGPAAYNKKL
SERRAKVVYDYLRNNGIDASRLIGPVGYGETHPIDTNKTPAGRAKNRRTELNVQN
>Mature_375_residues
MKKKILTAALLGGIAIIQVASAQEFDDRWYLAGSTGFNFQDKRRLTNDAPFFALGIGKFINPVWSIDGTLNYQNPQFKRN
KDLNWSQYGFSVDFRRHFIQDNRGWNPYLLLGAGYQRSEEEFDNTPNVNSPGERKKGGFAAKVGAGLQTTFRSRVAVRAE
VAYRGDHDKDSVRQPVAGVAAPSSKKWFGDTLASIGVVIPFGPAASSPTPPPAPPAPTPPPPPPAPSCAELDSDGDGVND
CDDKCPNSQPGQTIGPDGCPVPVSIDLKGVNFDFDKSKLRPDAVAVLKEATEILRRYPDLHVEVAGHTDSTGPAAYNKKL
SERRAKVVYDYLRNNGIDASRLIGPVGYGETHPIDTNKTPAGRAKNRRTELNVQN

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=104, Percent_Identity=40.3846153846154, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786962, Length=102, Percent_Identity=39.2156862745098, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 40818; Mature: 40818

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKILTAALLGGIAIIQVASAQEFDDRWYLAGSTGFNFQDKRRLTNDAPFFALGIGKFI
CCCHHHHHHHHHCEEEEEECCCCCCCCCEEEECCCCCCCHHHHHCCCCCCEEEEEHHHHH
NPVWSIDGTLNYQNPQFKRNKDLNWSQYGFSVDFRRHFIQDNRGWNPYLLLGAGYQRSEE
CHHEECCCEEECCCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHH
EFDNTPNVNSPGERKKGGFAAKVGAGLQTTFRSRVAVRAEVAYRGDHDKDSVRQPVAGVA
HCCCCCCCCCCCCCCCCCEEEHHCCCHHHHHHHHHEEEEEEEECCCCCHHHHHCCHHHCC
APSSKKWFGDTLASIGVVIPFGPAASSPTPPPAPPAPTPPPPPPAPSCAELDSDGDGVND
CCCCCCHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCC
CDDKCPNSQPGQTIGPDGCPVPVSIDLKGVNFDFDKSKLRPDAVAVLKEATEILRRYPDL
HHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCC
HVEVAGHTDSTGPAAYNKKLSERRAKVVYDYLRNNGIDASRLIGPVGYGETHPIDTNKTP
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCC
AGRAKNRRTELNVQN
CCCCCCCCCEECCCC
>Mature Secondary Structure
MKKKILTAALLGGIAIIQVASAQEFDDRWYLAGSTGFNFQDKRRLTNDAPFFALGIGKFI
CCCHHHHHHHHHCEEEEEECCCCCCCCCEEEECCCCCCCHHHHHCCCCCCEEEEEHHHHH
NPVWSIDGTLNYQNPQFKRNKDLNWSQYGFSVDFRRHFIQDNRGWNPYLLLGAGYQRSEE
CHHEECCCEEECCCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHH
EFDNTPNVNSPGERKKGGFAAKVGAGLQTTFRSRVAVRAEVAYRGDHDKDSVRQPVAGVA
HCCCCCCCCCCCCCCCCCEEEHHCCCHHHHHHHHHEEEEEEEECCCCCHHHHHCCHHHCC
APSSKKWFGDTLASIGVVIPFGPAASSPTPPPAPPAPTPPPPPPAPSCAELDSDGDGVND
CCCCCCHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCC
CDDKCPNSQPGQTIGPDGCPVPVSIDLKGVNFDFDKSKLRPDAVAVLKEATEILRRYPDL
HHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCC
HVEVAGHTDSTGPAAYNKKLSERRAKVVYDYLRNNGIDASRLIGPVGYGETHPIDTNKTP
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCC
AGRAKNRRTELNVQN
CCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]