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The map label for this gene is pglA [H]

Identifier: 182682092

GI number: 182682092

Start: 1742752

End: 1744386

Strand: Reverse

Name: pglA [H]

Synonym: XfasM23_1570

Alternate gene names: 182682092

Gene position: 1744386-1742752 (Counterclockwise)

Preceding gene: 182682093

Following gene: 182682081

Centisome position: 68.79

GC content: 52.91

Gene sequence:

>1635_bases
ATGAACCTTGACCGTTTCCTCCCCCTAGTGTTTGGACTGCACTGTTTTGCCGCAATGGGCGGCACTGCTGATGTCTTTCG
AGTGTCACAGCTGTCCTCATCAGATCTTTCCGAGCCGGTATCGGTGAAAACGCTATGGGGCGAGGTGCAACGTCCCAGCC
TACCTACCCACGTGTGTACGGTATTGCCTGCGCGCCTGACTCCTAAACATGGCTCCATTGACGCACTCGATGCCAATCCA
AAGGTTTCCAAGCCTGATACCAAGCGTTTACAGGATGCCATTGATGATTGTCCCGCCGGTAGCGCGGTCAAACTAGTGAT
CGACAGTCATCGCAAATCCGGCTTTTTGAGTGGGCCGTTGCACTTGAAATCTGGGGTGACGTTGTGGATTGACGAGGGTG
TTACGCTGTTTGCATCGCGCAACCCCAAGGATTACGACAAAGGCAATGGTACGTGCGGTACTGCAACGTCAACTCATGAG
TTCAGTTGTATGCCTTTGATCTCCGCGATCAACACCACCGGTAGCGGTATCGTGGGTGGGGGTGTCATTGATGGGCGTGG
CGGTAGCATTTTGACTGGCGGTAAGTATGCTCGGCAGCGTACCTGGTGGGACCTGGCCTATCAGAACAAGCGCCATGCAC
TGCATCAACAGGTACCACGGTTGATCCAAATCAGGGGCGGTAACGACTTCACGTTGTACCGTGTTGCCATCGAAAATGCA
CCTAACTTCCATGTGGTTGCTGATACGGTTAGCGGTGTCACCGCATGGGGCATCCGCATCCTGACCCCAAGCTTGGTCTA
TACCACGCCGGGGTACCACTGTCCGCCCGGTACCACGCCGGATGTTGTGACGCCGGCGACATGCTTTACCCCGGACACGG
TCAAGAACACCGATGGCTTTGATCCTGGGCAATCAAACCACGTGCTGCTAGCGTACTCCTACATCAGCACTGGGGATGAC
CACGTTGCCATCAAAGCGCGGGGCAAGATGCCAAGCTACGCTCTGAGCTTCTTACACAACCACTTTGGCTATGGACATGG
CATGTCCATCGGTAGCGACACCGAGTCAGGTGTACACGATATGGAAGTCAGTGATTTGAGTATCGACGGCTTTGACAGCC
CTAACAGCAATGGTTTGCAGATGAAATCTGATGCCGATCATGGCGGAGTGGTCGACCATGTCACCTACTCCAAAATATGC
ATGCGTCGTCTGAAACGTCCGCTGGCGTTTGACACCTTCTACAAACCTTCCAACGGGAATTCTTATCCACTCTTCAAAAA
TATCGTGCTGCAAGATATTCATGTGCTTGAAAGTCCTGTCTTTGGTGCTGGTCAGCTGCTTTTCATGGGGATCCTGGGCA
GTGGGAATAATCTGCCAATGACGCTGAGCATGGATAACGTCGTATTCGACGGTTTTTTGCCAACATTGATAGCGCCTCCC
AGCAGTGTCGTATTCGCCAATCCGCAGGCAGTGCATTTTCACTTCGGTCCCGGTCCGGTCAGTTTCGCTCCTTTGATTAC
CCCCTCGGTTGCTTACGATGTGACTGTGAGTGGAAGTCCAGGTGTGGGCAACCCATACGATTGTTCGGCGGCATTTATCA
ATTTTTCCAGCGTATTTCCTGATTCGCCTATCTAA

Upstream 100 bases:

>100_bases
TGTGACGGTGAGTCTCTCCCTAGTGCTGGCCTAACGATGTTGGTCCTTTTTGCCTCCGGTGCGACTGCTTCCTTACCCTT
TTTTTCAGAGTGTTTTTCTT

Downstream 100 bases:

>100_bases
TCAATGTGTGTCCAATCGCAGCTGTACAGCGGCCATGCGGATGTTGAAACAGCAGTGGCCGGACGTTGATCCAAGCAACA
CAGCTGCTGTCGGTCACAAT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: PGA; Pectinase [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MNLDRFLPLVFGLHCFAAMGGTADVFRVSQLSSSDLSEPVSVKTLWGEVQRPSLPTHVCTVLPARLTPKHGSIDALDANP
KVSKPDTKRLQDAIDDCPAGSAVKLVIDSHRKSGFLSGPLHLKSGVTLWIDEGVTLFASRNPKDYDKGNGTCGTATSTHE
FSCMPLISAINTTGSGIVGGGVIDGRGGSILTGGKYARQRTWWDLAYQNKRHALHQQVPRLIQIRGGNDFTLYRVAIENA
PNFHVVADTVSGVTAWGIRILTPSLVYTTPGYHCPPGTTPDVVTPATCFTPDTVKNTDGFDPGQSNHVLLAYSYISTGDD
HVAIKARGKMPSYALSFLHNHFGYGHGMSIGSDTESGVHDMEVSDLSIDGFDSPNSNGLQMKSDADHGGVVDHVTYSKIC
MRRLKRPLAFDTFYKPSNGNSYPLFKNIVLQDIHVLESPVFGAGQLLFMGILGSGNNLPMTLSMDNVVFDGFLPTLIAPP
SSVVFANPQAVHFHFGPGPVSFAPLITPSVAYDVTVSGSPGVGNPYDCSAAFINFSSVFPDSPI

Sequences:

>Translated_544_residues
MNLDRFLPLVFGLHCFAAMGGTADVFRVSQLSSSDLSEPVSVKTLWGEVQRPSLPTHVCTVLPARLTPKHGSIDALDANP
KVSKPDTKRLQDAIDDCPAGSAVKLVIDSHRKSGFLSGPLHLKSGVTLWIDEGVTLFASRNPKDYDKGNGTCGTATSTHE
FSCMPLISAINTTGSGIVGGGVIDGRGGSILTGGKYARQRTWWDLAYQNKRHALHQQVPRLIQIRGGNDFTLYRVAIENA
PNFHVVADTVSGVTAWGIRILTPSLVYTTPGYHCPPGTTPDVVTPATCFTPDTVKNTDGFDPGQSNHVLLAYSYISTGDD
HVAIKARGKMPSYALSFLHNHFGYGHGMSIGSDTESGVHDMEVSDLSIDGFDSPNSNGLQMKSDADHGGVVDHVTYSKIC
MRRLKRPLAFDTFYKPSNGNSYPLFKNIVLQDIHVLESPVFGAGQLLFMGILGSGNNLPMTLSMDNVVFDGFLPTLIAPP
SSVVFANPQAVHFHFGPGPVSFAPLITPSVAYDVTVSGSPGVGNPYDCSAAFINFSSVFPDSPI
>Mature_544_residues
MNLDRFLPLVFGLHCFAAMGGTADVFRVSQLSSSDLSEPVSVKTLWGEVQRPSLPTHVCTVLPARLTPKHGSIDALDANP
KVSKPDTKRLQDAIDDCPAGSAVKLVIDSHRKSGFLSGPLHLKSGVTLWIDEGVTLFASRNPKDYDKGNGTCGTATSTHE
FSCMPLISAINTTGSGIVGGGVIDGRGGSILTGGKYARQRTWWDLAYQNKRHALHQQVPRLIQIRGGNDFTLYRVAIENA
PNFHVVADTVSGVTAWGIRILTPSLVYTTPGYHCPPGTTPDVVTPATCFTPDTVKNTDGFDPGQSNHVLLAYSYISTGDD
HVAIKARGKMPSYALSFLHNHFGYGHGMSIGSDTESGVHDMEVSDLSIDGFDSPNSNGLQMKSDADHGGVVDHVTYSKIC
MRRLKRPLAFDTFYKPSNGNSYPLFKNIVLQDIHVLESPVFGAGQLLFMGILGSGNNLPMTLSMDNVVFDGFLPTLIAPP
SSVVFANPQAVHFHFGPGPVSFAPLITPSVAYDVTVSGSPGVGNPYDCSAAFINFSSVFPDSPI

Specific function: Contributes to the wilt disease production on tomato [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 28 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 58252; Mature: 58252

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDRFLPLVFGLHCFAAMGGTADVFRVSQLSSSDLSEPVSVKTLWGEVQRPSLPTHVCT
CCHHHHHHHHHHHHHHHHCCCCHHHEEEHHCCCCCCCCCEEEEEHHCCCCCCCCCHHHHH
VLPARLTPKHGSIDALDANPKVSKPDTKRLQDAIDDCPAGSAVKLVIDSHRKSGFLSGPL
HCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCE
HLKSGVTLWIDEGVTLFASRNPKDYDKGNGTCGTATSTHEFSCMPLISAINTTGSGIVGG
EECCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEC
GVIDGRGGSILTGGKYARQRTWWDLAYQNKRHALHQQVPRLIQIRGGNDFTLYRVAIENA
CEEECCCCCEEECCHHHHHCCEEHHHHCCHHHHHHHHCCEEEEEECCCCEEEEEEEECCC
PNFHVVADTVSGVTAWGIRILTPSLVYTTPGYHCPPGTTPDVVTPATCFTPDTVKNTDGF
CCEEEEEECCCCHHHCCEEEECCEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
DPGQSNHVLLAYSYISTGDDHVAIKARGKMPSYALSFLHNHFGYGHGMSIGSDTESGVHD
CCCCCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
MEVSDLSIDGFDSPNSNGLQMKSDADHGGVVDHVTYSKICMRRLKRPLAFDTFYKPSNGN
EEECEEEECCCCCCCCCCEEECCCCCCCCEEHHHHHHHHHHHHHCCCCEEECEECCCCCC
SYPLFKNIVLQDIHVLESPVFGAGQLLFMGILGSGNNLPMTLSMDNVVFDGFLPTLIAPP
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEECCEEECCCHHHHCCCC
SSVVFANPQAVHFHFGPGPVSFAPLITPSVAYDVTVSGSPGVGNPYDCSAAFINFSSVFP
CCEEEECCCEEEEEECCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCEEEEEHHHCCC
DSPI
CCCC
>Mature Secondary Structure
MNLDRFLPLVFGLHCFAAMGGTADVFRVSQLSSSDLSEPVSVKTLWGEVQRPSLPTHVCT
CCHHHHHHHHHHHHHHHHCCCCHHHEEEHHCCCCCCCCCEEEEEHHCCCCCCCCCHHHHH
VLPARLTPKHGSIDALDANPKVSKPDTKRLQDAIDDCPAGSAVKLVIDSHRKSGFLSGPL
HCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCE
HLKSGVTLWIDEGVTLFASRNPKDYDKGNGTCGTATSTHEFSCMPLISAINTTGSGIVGG
EECCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEC
GVIDGRGGSILTGGKYARQRTWWDLAYQNKRHALHQQVPRLIQIRGGNDFTLYRVAIENA
CEEECCCCCEEECCHHHHHCCEEHHHHCCHHHHHHHHCCEEEEEECCCCEEEEEEEECCC
PNFHVVADTVSGVTAWGIRILTPSLVYTTPGYHCPPGTTPDVVTPATCFTPDTVKNTDGF
CCEEEEEECCCCHHHCCEEEECCEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
DPGQSNHVLLAYSYISTGDDHVAIKARGKMPSYALSFLHNHFGYGHGMSIGSDTESGVHD
CCCCCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
MEVSDLSIDGFDSPNSNGLQMKSDADHGGVVDHVTYSKICMRRLKRPLAFDTFYKPSNGN
EEECEEEECCCCCCCCCCEEECCCCCCCCEEHHHHHHHHHHHHHCCCCEEECEECCCCCC
SYPLFKNIVLQDIHVLESPVFGAGQLLFMGILGSGNNLPMTLSMDNVVFDGFLPTLIAPP
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEECCEEECCCHHHHCCCC
SSVVFANPQAVHFHFGPGPVSFAPLITPSVAYDVTVSGSPGVGNPYDCSAAFINFSSVFP
CCEEEECCCEEEEEECCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCEEEEEHHHCCC
DSPI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2193922 [H]