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The map label for this gene is hemL

Identifier: 182681946

GI number: 182681946

Start: 1575949

End: 1577283

Strand: Direct

Name: hemL

Synonym: XfasM23_1419

Alternate gene names: 182681946

Gene position: 1575949-1577283 (Clockwise)

Preceding gene: 182681943

Following gene: 182681948

Centisome position: 62.15

GC content: 56.78

Gene sequence:

>1335_bases
ATGAATCATTCCCGCTCTCATGCCCTCTTTGTCCAGGCCCAAACACGCATCCCTGGTGGCGTTAATTCACCAGTCCGTGC
GTTCCGCTCAGTTGGCGGCGAACCGTTCTTCGTTGCACGTGCCGACGGCCCTTATCTGTTTGACGTCGACGGCCACCGCT
ACATTGATTATGTCGGCTCTTGGGGACCGATGATCGTCGGCCACAACCATCCAGCGGTGCGTGAAGCAGTGCAAGTGGCG
ATTAGCAACGGCCTGTCCTACGGTGCCCCATGTGCCGCCGAGGTGACCATGGCAGAAACCATTGCGCGGCTCGTGCCATC
CTGCGAAATGGTGCGCATGGTCAACTCTGGCACTGAAGCCACATTGTCAGCGATCCGACTAGCGCGCGGTGCCACTGGAC
GCAATTACATCGTTAAATTCGAAGGCTGTTACCACGGCCACGGCGACTCATTCCTAGTCAAAGGCGGCAGCGGCATGCTG
ACCCTTGGCATACCAAGCTCACCAGGGGTGCCTGCCGAGCTGAGCAAACTAACGATCACGTTGACTTACAACGATTTCGA
TGCGGCCACGGCACTGTTTGAGGAGATGGGACACCACATCGCGGCCGTCATTGTTGAACCTGTCATCGGCAACGCCAACT
GCATTCCGCCGCAACCAGGCTATCTGCAACATTTACGCACCCTGTGCACCCAGTACGCAGTGCTGTTGATCTTTGATGAA
GTCATGACCGGCTTTCGCGTCGCACTTGGCGGCGCGCAAGCGCTGTACGGAGTGACACCGGACCTGACCACCTTCGGTAA
GATCATCGGTGGCGGCATGCCGGTCGGTGCCTATGGTGGCCGCCGCGATCTGATGCAACACATTGCCCCAACCGGTCCAA
TCTATCAGGCAGGCACCCTCTCCGGTAACCCAGTGGCAATGGCGGCTGGTCTGGCCATGCTGGAGCTAATACAGGCTCCG
GATTTCTATACGCATCTGAGCAACGCCGCCGCAGCCCTGTGTACCGGTTTGCAACAAGCAGCCAGCCAGGCAGGCATTGC
AATGACCACACAACAGATTGGCGGTATGTTCGGACTGTTTTTTACCGATCAACAGGTGGAAACCTATGCCCAAGCGACCG
CCTGCAATACCGACCGCTTCAACCGCTTCTTCCACGCGATGCTGCAACGCGGCGTGTTTTTTGCCCCATCAGCGTATGAA
GCAGGATTTATCTCCAGCGCCCATTCACCAAACATCATAGAGACCACGCTGGAAGCTGCGCGTACAGCCTTCCAAACAAT
AGCCAACGAAGCAGCGATCCTGAGTAAATCAGAAACACCTATAAAAATGCGATGA

Upstream 100 bases:

>100_bases
TGTCACTAATGCGACAGAGCCGTCGCTGCCGCAACAATAGACATGCTGTACGCATCCACTCATTCATCCCACTACCCGTG
CTGCAAGGTCTCCGCTCCCC

Downstream 100 bases:

>100_bases
CTAAATAAAGTAAAACGCACTTCAGCAACGCACCAAGAAAGTGAGCCTATTGCAGACATTACTGTTTTCAGCAACACAGC
ATATTGCCGCACCGCTCAAC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MNHSRSHALFVQAQTRIPGGVNSPVRAFRSVGGEPFFVARADGPYLFDVDGHRYIDYVGSWGPMIVGHNHPAVREAVQVA
ISNGLSYGAPCAAEVTMAETIARLVPSCEMVRMVNSGTEATLSAIRLARGATGRNYIVKFEGCYHGHGDSFLVKGGSGML
TLGIPSSPGVPAELSKLTITLTYNDFDAATALFEEMGHHIAAVIVEPVIGNANCIPPQPGYLQHLRTLCTQYAVLLIFDE
VMTGFRVALGGAQALYGVTPDLTTFGKIIGGGMPVGAYGGRRDLMQHIAPTGPIYQAGTLSGNPVAMAAGLAMLELIQAP
DFYTHLSNAAAALCTGLQQAASQAGIAMTTQQIGGMFGLFFTDQQVETYAQATACNTDRFNRFFHAMLQRGVFFAPSAYE
AGFISSAHSPNIIETTLEAARTAFQTIANEAAILSKSETPIKMR

Sequences:

>Translated_444_residues
MNHSRSHALFVQAQTRIPGGVNSPVRAFRSVGGEPFFVARADGPYLFDVDGHRYIDYVGSWGPMIVGHNHPAVREAVQVA
ISNGLSYGAPCAAEVTMAETIARLVPSCEMVRMVNSGTEATLSAIRLARGATGRNYIVKFEGCYHGHGDSFLVKGGSGML
TLGIPSSPGVPAELSKLTITLTYNDFDAATALFEEMGHHIAAVIVEPVIGNANCIPPQPGYLQHLRTLCTQYAVLLIFDE
VMTGFRVALGGAQALYGVTPDLTTFGKIIGGGMPVGAYGGRRDLMQHIAPTGPIYQAGTLSGNPVAMAAGLAMLELIQAP
DFYTHLSNAAAALCTGLQQAASQAGIAMTTQQIGGMFGLFFTDQQVETYAQATACNTDRFNRFFHAMLQRGVFFAPSAYE
AGFISSAHSPNIIETTLEAARTAFQTIANEAAILSKSETPIKMR
>Mature_444_residues
MNHSRSHALFVQAQTRIPGGVNSPVRAFRSVGGEPFFVARADGPYLFDVDGHRYIDYVGSWGPMIVGHNHPAVREAVQVA
ISNGLSYGAPCAAEVTMAETIARLVPSCEMVRMVNSGTEATLSAIRLARGATGRNYIVKFEGCYHGHGDSFLVKGGSGML
TLGIPSSPGVPAELSKLTITLTYNDFDAATALFEEMGHHIAAVIVEPVIGNANCIPPQPGYLQHLRTLCTQYAVLLIFDE
VMTGFRVALGGAQALYGVTPDLTTFGKIIGGGMPVGAYGGRRDLMQHIAPTGPIYQAGTLSGNPVAMAAGLAMLELIQAP
DFYTHLSNAAAALCTGLQQAASQAGIAMTTQQIGGMFGLFFTDQQVETYAQATACNTDRFNRFFHAMLQRGVFFAPSAYE
AGFISSAHSPNIIETTLEAARTAFQTIANEAAILSKSETPIKMR

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=324, Percent_Identity=28.7037037037037, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI24119277, Length=362, Percent_Identity=27.3480662983425, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI284507298, Length=238, Percent_Identity=29.8319327731092, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=60.9411764705882, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI1789016, Length=352, Percent_Identity=31.5340909090909, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788044, Length=299, Percent_Identity=33.4448160535117, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787560, Length=350, Percent_Identity=29.4285714285714, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789759, Length=336, Percent_Identity=28.2738095238095, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI145693181, Length=333, Percent_Identity=28.8288288288288, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1786991, Length=380, Percent_Identity=26.5789473684211, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25144271, Length=307, Percent_Identity=29.3159609120521, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=371, Percent_Identity=25.8760107816712, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI25144274, Length=142, Percent_Identity=35.9154929577465, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=305, Percent_Identity=27.8688524590164, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324432, Length=368, Percent_Identity=24.7282608695652, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21357415, Length=297, Percent_Identity=28.956228956229, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_XYLF2 (B2I6C2)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830106.1
- ProteinModelPortal:   B2I6C2
- SMR:   B2I6C2
- GeneID:   6202331
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1419
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 47176; Mature: 47176

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSRSHALFVQAQTRIPGGVNSPVRAFRSVGGEPFFVARADGPYLFDVDGHRYIDYVGS
CCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCEEEEECCC
WGPMIVGHNHPAVREAVQVAISNGLSYGAPCAAEVTMAETIARLVPSCEMVRMVNSGTEA
CCCEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHH
TLSAIRLARGATGRNYIVKFEGCYHGHGDSFLVKGGSGMLTLGIPSSPGVPAELSKLTIT
HHHHHHHHCCCCCCCEEEEEECEECCCCCEEEEECCCCEEEEECCCCCCCCCEEEEEEEE
LTYNDFDAATALFEEMGHHIAAVIVEPVIGNANCIPPQPGYLQHLRTLCTQYAVLLIFDE
EEECCHHHHHHHHHHHHHHEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VMTGFRVALGGAQALYGVTPDLTTFGKIIGGGMPVGAYGGRRDLMQHIAPTGPIYQAGTL
HHHHHHHHHCCCHHEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCC
SGNPVAMAAGLAMLELIQAPDFYTHLSNAAAALCTGLQQAASQAGIAMTTQQIGGMFGLF
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCEEEEE
FTDQQVETYAQATACNTDRFNRFFHAMLQRGVFFAPSAYEAGFISSAHSPNIIETTLEAA
EECHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHHHHHH
RTAFQTIANEAAILSKSETPIKMR
HHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNHSRSHALFVQAQTRIPGGVNSPVRAFRSVGGEPFFVARADGPYLFDVDGHRYIDYVGS
CCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCEEEEECCC
WGPMIVGHNHPAVREAVQVAISNGLSYGAPCAAEVTMAETIARLVPSCEMVRMVNSGTEA
CCCEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHH
TLSAIRLARGATGRNYIVKFEGCYHGHGDSFLVKGGSGMLTLGIPSSPGVPAELSKLTIT
HHHHHHHHCCCCCCCEEEEEECEECCCCCEEEEECCCCEEEEECCCCCCCCCEEEEEEEE
LTYNDFDAATALFEEMGHHIAAVIVEPVIGNANCIPPQPGYLQHLRTLCTQYAVLLIFDE
EEECCHHHHHHHHHHHHHHEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VMTGFRVALGGAQALYGVTPDLTTFGKIIGGGMPVGAYGGRRDLMQHIAPTGPIYQAGTL
HHHHHHHHHCCCHHEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCC
SGNPVAMAAGLAMLELIQAPDFYTHLSNAAAALCTGLQQAASQAGIAMTTQQIGGMFGLF
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCEEEEE
FTDQQVETYAQATACNTDRFNRFFHAMLQRGVFFAPSAYEAGFISSAHSPNIIETTLEAA
EECHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHHHHHH
RTAFQTIANEAAILSKSETPIKMR
HHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA