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The map label for this gene is kdsB

Identifier: 182681948

GI number: 182681948

Start: 1578725

End: 1579498

Strand: Direct

Name: kdsB

Synonym: XfasM23_1421

Alternate gene names: 182681948

Gene position: 1578725-1579498 (Clockwise)

Preceding gene: 182681946

Following gene: 182681949

Centisome position: 62.26

GC content: 57.24

Gene sequence:

>774_bases
ATGAGTCTCGAAATCGTGCCTTTTGTAGTTGCTATTCCCGCACGTTTTTCCGCATCACGACTGCCTGGAAAACCGCTACG
CCTGCTTGGTGGTCGCCCACTGATTCACAGGGTAGCCGAACGGGCACTTAGTACAGGGGCACGAGAAGTATGGGTTGCAA
CCGACGATGTACGCATCGCTGAGGCCGTTGCTTCTCTGGACGGTGTCCACGTGGCGATGACCGCAAACACTCACCTATCC
GGGAGCGACCGTTTAGCCGAGTGCGCACGCATCGCCGGATGGGATCCTGAGGTGTGCGTGGTCAACCTACAGGGCGATGA
GCCGTTCGCACCAGCGGCGGGGATCCGTGCTGTAGCGGCATTGCTGCATCACAGCAACGCCGACATGGCAACGCTCGCAA
CGACCATCGACAAGAGCGAAGACCTGTTCAACCCGAATATCGTCAAACTAGTGTGCAATACACATGGAGAAGCGTTGTAC
TTCAGCCGTGCACCGATCCCATGGAATCGGGATACCTTCGCCACAACGCGTGAACCAACGCCATTGGGTCCGTGGCTACG
CCACATCGGTCTCTATGCCTGCAACGCTGGGTTCCTGCAACGCTTCACCACGATGCAACCGGGGACCTTGGAGCAGATTG
AATCGTTGGAACAACTGCGTGTGCTGGAAGCAGGGCATCGGATCGCGGTCAGGATCACTCCGGAACATTTCCCGCCCGGT
ATCGATACCCCAGAAGACTTAGCCAAAGCCGAGAAGGCATTGGAGGATGTATGA

Upstream 100 bases:

>100_bases
ATCCACATGAATCAGCCGAAGTATGAATGTGCCCCACATGAGCACAGAAAAACGCCGTTGTTTTTGATGTCGTTTGACTT
GATTTCATAGGAAAGTCTGG

Downstream 100 bases:

>100_bases
GATTGTTGGTCGTTTGCCTGGGTAACATTTGTCGCTCCCCAATGGGCGAGGGAGCATTACGCAAGCACATCACCGCAAGC
GGTAAAAACGAGGAAATCGC

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSLEIVPFVVAIPARFSASRLPGKPLRLLGGRPLIHRVAERALSTGAREVWVATDDVRIAEAVASLDGVHVAMTANTHLS
GSDRLAECARIAGWDPEVCVVNLQGDEPFAPAAGIRAVAALLHHSNADMATLATTIDKSEDLFNPNIVKLVCNTHGEALY
FSRAPIPWNRDTFATTREPTPLGPWLRHIGLYACNAGFLQRFTTMQPGTLEQIESLEQLRVLEAGHRIAVRITPEHFPPG
IDTPEDLAKAEKALEDV

Sequences:

>Translated_257_residues
MSLEIVPFVVAIPARFSASRLPGKPLRLLGGRPLIHRVAERALSTGAREVWVATDDVRIAEAVASLDGVHVAMTANTHLS
GSDRLAECARIAGWDPEVCVVNLQGDEPFAPAAGIRAVAALLHHSNADMATLATTIDKSEDLFNPNIVKLVCNTHGEALY
FSRAPIPWNRDTFATTREPTPLGPWLRHIGLYACNAGFLQRFTTMQPGTLEQIESLEQLRVLEAGHRIAVRITPEHFPPG
IDTPEDLAKAEKALEDV
>Mature_256_residues
SLEIVPFVVAIPARFSASRLPGKPLRLLGGRPLIHRVAERALSTGAREVWVATDDVRIAEAVASLDGVHVAMTANTHLSG
SDRLAECARIAGWDPEVCVVNLQGDEPFAPAAGIRAVAALLHHSNADMATLATTIDKSEDLFNPNIVKLVCNTHGEALYF
SRAPIPWNRDTFATTREPTPLGPWLRHIGLYACNAGFLQRFTTMQPGTLEQIESLEQLRVLEAGHRIAVRITPEHFPPGI
DTPEDLAKAEKALEDV

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=242, Percent_Identity=49.1735537190083, Blast_Score=216, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_XYLF2 (B2I6C4)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830108.1
- GeneID:   6202325
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1421
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- ProtClustDB:   PRK05450
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 27942; Mature: 27811

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEIVPFVVAIPARFSASRLPGKPLRLLGGRPLIHRVAERALSTGAREVWVATDDVRIA
CCCEEEEEEEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCHHHH
EAVASLDGVHVAMTANTHLSGSDRLAECARIAGWDPEVCVVNLQGDEPFAPAAGIRAVAA
HHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHH
LLHHSNADMATLATTIDKSEDLFNPNIVKLVCNTHGEALYFSRAPIPWNRDTFATTREPT
HHHCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCC
PLGPWLRHIGLYACNAGFLQRFTTMQPGTLEQIESLEQLRVLEAGHRIAVRITPEHFPPG
CCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
IDTPEDLAKAEKALEDV
CCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLEIVPFVVAIPARFSASRLPGKPLRLLGGRPLIHRVAERALSTGAREVWVATDDVRIA
CCEEEEEEEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCHHHH
EAVASLDGVHVAMTANTHLSGSDRLAECARIAGWDPEVCVVNLQGDEPFAPAAGIRAVAA
HHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHH
LLHHSNADMATLATTIDKSEDLFNPNIVKLVCNTHGEALYFSRAPIPWNRDTFATTREPT
HHHCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCC
PLGPWLRHIGLYACNAGFLQRFTTMQPGTLEQIESLEQLRVLEAGHRIAVRITPEHFPPG
CCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
IDTPEDLAKAEKALEDV
CCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA