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The map label for this gene is pilT [H]

Identifier: 182681763

GI number: 182681763

Start: 1353613

End: 1354647

Strand: Direct

Name: pilT [H]

Synonym: XfasM23_1221

Alternate gene names: 182681763

Gene position: 1353613-1354647 (Clockwise)

Preceding gene: 182681759

Following gene: 182681764

Centisome position: 53.38

GC content: 49.86

Gene sequence:

>1035_bases
ATGGATATTGCTGAACTGTTAGCGTTTTCAGTCAAAAACAATGCTTCGGACCTGCACCTGTCAGCAGGCCTGCCACCAAT
GATCAGGGTGGACGGCGATGTACGCCGTATCAATATCCCCGCATTCGACCATAAGCAGGTGCAATCGCTAATTTACGACA
TCATGTCGGATAAGCAACGACGCGACTATGAGGAAGCCCTGGAGACGGATTTTTCGTTCGAAATCCCTTCACTCTCCCGC
TTCCGTGTCAATGCATTCAACCAAGAGCGTGGTGCTGGTGCCGTGTTTCGCACCATTCCCAATAAGGTGATGACATTGGA
CGAACTCGGCTGTTTGCCGGTGTTCCGCAAACTCATTGAAGAACCGCAGGGGTTGATTCTGGTGACTGGGCCGACTGGCT
CTGGAAAATCGACCACCCTCGCAGCCATGATCGACCATATCAACAAAAATGCTCACGGGCATATTCTCACGATAGAGGAC
CCGATTGAGTTCGTTCACACCTCACAGAAGTGCTTGATTAACCAACGTGAGGTACATCGCGATACTCACGACTTCAACAA
AGCACTCAGCTCAGCACTGCGCGAAGATCCAGACATCATCTTGATTGGAGAATTGCGTGACCTAGAAACGATCCGTCTAG
CACTGACTGCAGCCGAAACTGGCCACTTGGTATTTGGCACCCTGCACACCAATTCAGCAGCTAAAAGTATTAACCGCATC
ATCGACGTGTTTCCCGCTGCTGAAAAACCGATGGTCCGCTCTATGCTTTCCGAATCGCTCTGCGGGATCATCTCGCAAAT
GTTGTTGAAAAAAGTTGGTGGTGGACGTACCGCAGCCTGGGAAATTATGGTTGGCACCCCCGCAATCCGAAATTTGATCC
GTGAGGATAAAGTCGCACAGATGTATTCATCCATCCAAACTGGCCAGCAATATGGCATGCAGACCCTTGACCAGCATTTA
CAGGATCTCATCAAACGCAACCTGATCACACGACAGCAAGCACGTGAATACGCCAAGGATAAAGCGAATTTTTGA

Upstream 100 bases:

>100_bases
AAGCACTTGATAACTGCACACCACCACTGTGATGCGTGCGCTATACTGCGCGCCGAATAGGATTCTTCCATTCGTCGTCT
ATGCAAGGGGCAAAGAACAT

Downstream 100 bases:

>100_bases
TTAAATACCGCACTATCACACATAAGATACAAACTAAGCAGTTCCCCCTTCAAAACGCCCTAGTGGCGCCCTAGTACAGT
GGACATTGAAAGCTGGAGAC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDVRRINIPAFDHKQVQSLIYDIMSDKQRRDYEEALETDFSFEIPSLSR
FRVNAFNQERGAGAVFRTIPNKVMTLDELGCLPVFRKLIEEPQGLILVTGPTGSGKSTTLAAMIDHINKNAHGHILTIED
PIEFVHTSQKCLINQREVHRDTHDFNKALSSALREDPDIILIGELRDLETIRLALTAAETGHLVFGTLHTNSAAKSINRI
IDVFPAAEKPMVRSMLSESLCGIISQMLLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLIKRNLITRQQAREYAKDKANF

Sequences:

>Translated_344_residues
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDVRRINIPAFDHKQVQSLIYDIMSDKQRRDYEEALETDFSFEIPSLSR
FRVNAFNQERGAGAVFRTIPNKVMTLDELGCLPVFRKLIEEPQGLILVTGPTGSGKSTTLAAMIDHINKNAHGHILTIED
PIEFVHTSQKCLINQREVHRDTHDFNKALSSALREDPDIILIGELRDLETIRLALTAAETGHLVFGTLHTNSAAKSINRI
IDVFPAAEKPMVRSMLSESLCGIISQMLLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLIKRNLITRQQAREYAKDKANF
>Mature_344_residues
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDVRRINIPAFDHKQVQSLIYDIMSDKQRRDYEEALETDFSFEIPSLSR
FRVNAFNQERGAGAVFRTIPNKVMTLDELGCLPVFRKLIEEPQGLILVTGPTGSGKSTTLAAMIDHINKNAHGHILTIED
PIEFVHTSQKCLINQREVHRDTHDFNKALSSALREDPDIILIGELRDLETIRLALTAAETGHLVFGTLHTNSAAKSINRI
IDVFPAAEKPMVRSMLSESLCGIISQMLLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLIKRNLITRQQAREYAKDKANF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=47.9750778816199, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1789723, Length=285, Percent_Identity=35.7894736842105, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786296, Length=281, Percent_Identity=33.8078291814947, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38498; Mature: 38498

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDVRRINIPAFDHKQVQSLIYDIMSDKQR
CCHHHHHHHHHCCCCCCEEEECCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHH
RDYEEALETDFSFEIPSLSRFRVNAFNQERGAGAVFRTIPNKVMTLDELGCLPVFRKLIE
CCHHHHHCCCCCCCCCCCCHHEECCCCCCCCCCHHHHHCCHHHHHHHHCCHHHHHHHHHC
EPQGLILVTGPTGSGKSTTLAAMIDHINKNAHGHILTIEDPIEFVHTSQKCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCHHHHHCHHHHHH
DTHDFNKALSSALREDPDIILIGELRDLETIRLALTAAETGHLVFGTLHTNSAAKSINRI
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEEECCHHHHHHHHH
IDVFPAAEKPMVRSMLSESLCGIISQMLLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHH
MYSSIQTGQQYGMQTLDQHLQDLIKRNLITRQQAREYAKDKANF
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDVRRINIPAFDHKQVQSLIYDIMSDKQR
CCHHHHHHHHHCCCCCCEEEECCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHH
RDYEEALETDFSFEIPSLSRFRVNAFNQERGAGAVFRTIPNKVMTLDELGCLPVFRKLIE
CCHHHHHCCCCCCCCCCCCHHEECCCCCCCCCCHHHHHCCHHHHHHHHCCHHHHHHHHHC
EPQGLILVTGPTGSGKSTTLAAMIDHINKNAHGHILTIEDPIEFVHTSQKCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCHHHHHCHHHHHH
DTHDFNKALSSALREDPDIILIGELRDLETIRLALTAAETGHLVFGTLHTNSAAKSINRI
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEEECCCCCEEEEEEECCHHHHHHHHH
IDVFPAAEKPMVRSMLSESLCGIISQMLLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHH
MYSSIQTGQQYGMQTLDQHLQDLIKRNLITRQQAREYAKDKANF
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]