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The map label for this gene is pilT [H]

Identifier: 182681764

GI number: 182681764

Start: 1354813

End: 1355943

Strand: Direct

Name: pilT [H]

Synonym: XfasM23_1222

Alternate gene names: 182681764

Gene position: 1354813-1355943 (Clockwise)

Preceding gene: 182681763

Following gene: 182681766

Centisome position: 53.43

GC content: 46.33

Gene sequence:

>1131_bases
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCACATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Upstream 100 bases:

>100_bases
GCGCCCTAGTACAGTGGACATTGAAAGCTGGAGACACACCGCAACATGGAGCGCCTGCTCCTCTCCAGCTATCGGCATAG
CAGCAAAGGAGCAACACCCG

Downstream 100 bases:

>100_bases
ATCTATTATGAGGTTATATAATCAATAGTGATGATTAATACCTTCATAATAACCACCCGCCGACGATGCAATTAGAGTGA
CACACCTAGATATCTATCCA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DHIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Sequences:

>Translated_376_residues
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DHIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS
>Mature_375_residues
TTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITSI
GRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVTI
EDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAID
HIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTFD
QSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=33.0218068535826, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1789723, Length=153, Percent_Identity=39.2156862745098, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1786296, Length=251, Percent_Identity=26.2948207171315, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42461; Mature: 42330

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSE
CCCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCCCCEECCCCCCCCCHHHHHHHHH
AQREEFEKVHECNFAIDITSIGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKE
HHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHH
LVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVTIEDPIEFIHKHEKCIITQRE
HHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHCCCCEEEEHHH
IGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
CCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHH
DHIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVG
HHHHHHCCHHHHHHEEHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EIHKLKEVMQNHNDLGMTTFDQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEEECCCCCH
KTLSQGFDGIEIAEMS
HHHHCCCCCEEEEECC
>Mature Secondary Structure 
TTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSE
CCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCCCCEECCCCCCCCCHHHHHHHHH
AQREEFEKVHECNFAIDITSIGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKE
HHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHH
LVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVTIEDPIEFIHKHEKCIITQRE
HHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHCCCCEEEEHHH
IGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
CCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHH
DHIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVG
HHHHHHCCHHHHHHEEHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EIHKLKEVMQNHNDLGMTTFDQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEEECCCCCH
KTLSQGFDGIEIAEMS
HHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]