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The map label for this gene is purD [H]

Identifier: 182681425

GI number: 182681425

Start: 1034736

End: 1036028

Strand: Reverse

Name: purD [H]

Synonym: XfasM23_0875

Alternate gene names: 182681425

Gene position: 1036028-1034736 (Counterclockwise)

Preceding gene: 182681426

Following gene: 182681424

Centisome position: 40.86

GC content: 57.46

Gene sequence:

>1293_bases
ATGAAACTCCTTGTTATTGGTTCTGGTGGCCGTGAACATGCGCTGGTCTGGAAACTCGCCCACTCTCGACGTGTCTCTGA
AATCATCGTGGCGCCCGGTAACGCAGGCACTGCTACAGAAACTAAATGCCGTAATGCACCCGTCCAGGTCACCGACCTGG
ACGGGCTGTTGGCGTTGGCACAGCGTGAAGCGGTGAACATCACCGTGGTAGGTCCTGAAGTGCCACTAGTAGCGGGCATT
GTTGATTGCTTCCGCGCCGCCGGGATGCGTATTTTTGGGCCGACAGCCGCCGCCGCACAGTTAGAGGGCAGTAAAGCCTA
TGCAAAAGACTTTATGGCTCGTCACGGTATCCCCACGGCGCGCTATGCGGTGCATACCAATGTCGACGCTGCAATCAGCG
ATGTGCGGCAGCAGGGTGCCCCCATCGTCATCAAAGCCGATGGGTTGGCGGCAGGTAAAGGTGTCATTGTGGCGATGACC
GTGACCGAGGCTGAAGCGGCCATACGTGACATGTTGTCCGGCAATGCGTTCGGTCATGCAGGGGCACGGGTGGTCATTGA
GGAATATCTTGATGGAGAAGAAGCCAGTTTCATCTCAATGGTCGACGGCACACACGCGCTGCCGATGGCCACCTCGCAAG
ATCATAAGCGCGTCAGTGATGGCGACATAGGCCCGAATACGGGGGGCATGGGTGCTTACTCACCAGCACCAATCATCACG
GACGAGATCCATGCGCGGGTGATGCGTGAGATCGTTAACCCAACCGTGGCGGGGATGATCGCTGACGGTACGCCGTTCAT
GGGATTTCTTTACGCTGGTCTGATGATTGATGTCCATGGCGCTCCCAAAGTCATTGAGTTCAATGTACGTTTTGGGGATC
CGGAAACTCAACCGGTAATGATGCGTTTGCAATCAGATCTGTTGGATCTGATTGAAGCGGCACTCAATGGCGATCTTGAC
AAGGTGCAGGCGCAGTGGGACCCGCACCCATCGCTAGGTGTGGTGATGGCGGCACGGCCGTATCCTGAAATGCCTATCAC
CGGGGAGGTCATCTCTGGCTTGGACGCGCTCCCAGCCAATGTGAAGGTATTTCATGCGGGCACCGCTCTCGACGTGGCGG
GACGCGTCGTCACGGCAGGTGGGCGTGTGCTTTGTGTAACCGCACTCGGAAGCAATGTGTCCGAAGCACAGCGGCATGCC
TATGGCGGTGTGGCATCGCTGCATTGGGCCAACGCATTCCAGCGCAGTGATATTGGCTGGCGCGCCATCATGCGGGAGCA
CACTGTACGTTGA

Upstream 100 bases:

>100_bases
CAAAACCAACCGTGACAGACCAGGACGCTTCCCACAACACCATTGATGCGAACGCCATGCTGCAATCCACTTCTCTCTCC
ATTCTTTCATTTCATTCCCT

Downstream 100 bases:

>100_bases
GCGTCCATGGCCCTGCGTGACTCAGGAGCAGAATCGGTTTGTATCTATATAGGACCTGGGAACGGCTTGCTCATGAACGC
GCGCTGGCTAGCAACACTAC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKLLVIGSGGREHALVWKLAHSRRVSEIIVAPGNAGTATETKCRNAPVQVTDLDGLLALAQREAVNITVVGPEVPLVAGI
VDCFRAAGMRIFGPTAAAAQLEGSKAYAKDFMARHGIPTARYAVHTNVDAAISDVRQQGAPIVIKADGLAAGKGVIVAMT
VTEAEAAIRDMLSGNAFGHAGARVVIEEYLDGEEASFISMVDGTHALPMATSQDHKRVSDGDIGPNTGGMGAYSPAPIIT
DEIHARVMREIVNPTVAGMIADGTPFMGFLYAGLMIDVHGAPKVIEFNVRFGDPETQPVMMRLQSDLLDLIEAALNGDLD
KVQAQWDPHPSLGVVMAARPYPEMPITGEVISGLDALPANVKVFHAGTALDVAGRVVTAGGRVLCVTALGSNVSEAQRHA
YGGVASLHWANAFQRSDIGWRAIMREHTVR

Sequences:

>Translated_430_residues
MKLLVIGSGGREHALVWKLAHSRRVSEIIVAPGNAGTATETKCRNAPVQVTDLDGLLALAQREAVNITVVGPEVPLVAGI
VDCFRAAGMRIFGPTAAAAQLEGSKAYAKDFMARHGIPTARYAVHTNVDAAISDVRQQGAPIVIKADGLAAGKGVIVAMT
VTEAEAAIRDMLSGNAFGHAGARVVIEEYLDGEEASFISMVDGTHALPMATSQDHKRVSDGDIGPNTGGMGAYSPAPIIT
DEIHARVMREIVNPTVAGMIADGTPFMGFLYAGLMIDVHGAPKVIEFNVRFGDPETQPVMMRLQSDLLDLIEAALNGDLD
KVQAQWDPHPSLGVVMAARPYPEMPITGEVISGLDALPANVKVFHAGTALDVAGRVVTAGGRVLCVTALGSNVSEAQRHA
YGGVASLHWANAFQRSDIGWRAIMREHTVR
>Mature_430_residues
MKLLVIGSGGREHALVWKLAHSRRVSEIIVAPGNAGTATETKCRNAPVQVTDLDGLLALAQREAVNITVVGPEVPLVAGI
VDCFRAAGMRIFGPTAAAAQLEGSKAYAKDFMARHGIPTARYAVHTNVDAAISDVRQQGAPIVIKADGLAAGKGVIVAMT
VTEAEAAIRDMLSGNAFGHAGARVVIEEYLDGEEASFISMVDGTHALPMATSQDHKRVSDGDIGPNTGGMGAYSPAPIIT
DEIHARVMREIVNPTVAGMIADGTPFMGFLYAGLMIDVHGAPKVIEFNVRFGDPETQPVMMRLQSDLLDLIEAALNGDLD
KVQAQWDPHPSLGVVMAARPYPEMPITGEVISGLDALPANVKVFHAGTALDVAGRVVTAGGRVLCVTALGSNVSEAQRHA
YGGVASLHWANAFQRSDIGWRAIMREHTVR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=48.4705882352941, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=48.4705882352941, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=48.4705882352941, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=48.4705882352941, Blast_Score=392, Evalue=1e-109,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=64.5687645687646, Blast_Score=556, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=38.4433962264151, Blast_Score=282, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6321203, Length=446, Percent_Identity=43.2735426008969, Blast_Score=338, Evalue=1e-93,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=43.953488372093, Blast_Score=324, Evalue=8e-89,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=44.1860465116279, Blast_Score=323, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45339; Mature: 45339

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVIGSGGREHALVWKLAHSRRVSEIIVAPGNAGTATETKCRNAPVQVTDLDGLLALA
CEEEEEECCCCCCEEEEEEHHCCCCEEEEEECCCCCCCCCHHHCCCCEEEECCHHHHHHH
QREAVNITVVGPEVPLVAGIVDCFRAAGMRIFGPTAAAAQLEGSKAYAKDFMARHGIPTA
CCCEEEEEEECCCCHHHHHHHHHHHHCCCEEECCCCHHHHCCCCHHHHHHHHHHCCCCCC
RYAVHTNVDAAISDVRQQGAPIVIKADGLAAGKGVIVAMTVTEAEAAIRDMLSGNAFGHA
EEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCCC
GARVVIEEYLDGEEASFISMVDGTHALPMATSQDHKRVSDGDIGPNTGGMGAYSPAPIIT
CHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
DEIHARVMREIVNPTVAGMIADGTPFMGFLYAGLMIDVHGAPKVIEFNVRFGDPETQPVM
HHHHHHHHHHHHCCHHEEEEECCCCHHHHHHHCEEEEECCCCEEEEEEEEECCCCCCHHH
MRLQSDLLDLIEAALNGDLDKVQAQWDPHPSLGVVMAARPYPEMPITGEVISGLDALPAN
HHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCC
VKVFHAGTALDVAGRVVTAGGRVLCVTALGSNVSEAQRHAYGGVASLHWANAFQRSDIGW
EEEEECCCCHHHCCEEEECCCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHH
RAIMREHTVR
HHHHHHHCCC
>Mature Secondary Structure
MKLLVIGSGGREHALVWKLAHSRRVSEIIVAPGNAGTATETKCRNAPVQVTDLDGLLALA
CEEEEEECCCCCCEEEEEEHHCCCCEEEEEECCCCCCCCCHHHCCCCEEEECCHHHHHHH
QREAVNITVVGPEVPLVAGIVDCFRAAGMRIFGPTAAAAQLEGSKAYAKDFMARHGIPTA
CCCEEEEEEECCCCHHHHHHHHHHHHCCCEEECCCCHHHHCCCCHHHHHHHHHHCCCCCC
RYAVHTNVDAAISDVRQQGAPIVIKADGLAAGKGVIVAMTVTEAEAAIRDMLSGNAFGHA
EEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCCC
GARVVIEEYLDGEEASFISMVDGTHALPMATSQDHKRVSDGDIGPNTGGMGAYSPAPIIT
CHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
DEIHARVMREIVNPTVAGMIADGTPFMGFLYAGLMIDVHGAPKVIEFNVRFGDPETQPVM
HHHHHHHHHHHHCCHHEEEEECCCCHHHHHHHCEEEEECCCCEEEEEEEEECCCCCCHHH
MRLQSDLLDLIEAALNGDLDKVQAQWDPHPSLGVVMAARPYPEMPITGEVISGLDALPAN
HHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCC
VKVFHAGTALDVAGRVVTAGGRVLCVTALGSNVSEAQRHAYGGVASLHWANAFQRSDIGW
EEEEECCCCHHHCCEEEECCCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHH
RAIMREHTVR
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]