Click here to switch to the map view.

The map label for this gene is purH

Identifier: 182681426

GI number: 182681426

Start: 1036221

End: 1037804

Strand: Reverse

Name: purH

Synonym: XfasM23_0876

Alternate gene names: 182681426

Gene position: 1037804-1036221 (Counterclockwise)

Preceding gene: 182681427

Following gene: 182681425

Centisome position: 40.93

GC content: 54.99

Gene sequence:

>1584_bases
ATGGCATCCGATTTTCTGCCCGTGCATCGTGCACTGTTATCTGTTTCCGACAAAACAGGGTTGGTCGAGTTGGCACGCGT
GCTGCTGGCCTACAACATTGAATTACTGTCTACCGGTGGGACCGCGACAATCATCCGCGAGGCTGGTCTCCCAGTGCAGG
ATGTGGCCGACCTGACAGACTTCCCGGAAATGATGGACGGCCGCGTAAAAACACTGCACCCCGTGGTACACGGTGGTTTG
CTCGGACGTGCCGGCATCGACGACGCCGTGATGGCCAAACACGGCATCGCACCAATCGACCTGCTGATAGTGAATCTGTA
TCCCTTCGAACAGATCACTGCCAAAAAAGATTGCACGCTGGCCGATGCAGTGGACACCATTGATATTGGTGGCCCGGCGA
TGCTGCGCTCGGCGGCAAAGAATTTCGCGCGTGTGGCTGTGGCAACGTCACCGGATCAATATCCTGATCTGCTTGCTGAA
CTGCAGGCGCACCACGGCCAGCTTTCCGCCGAAAAGCGTTTCGCATTGGCCGTGGCGGCGTTCAACCACGTTGCCCAGTA
CGATGCGGCCATCAGCAACTATCTTTCGAGCGTGTCCGACATGCACACAACGTTGCCGTTGCGCCATGAATTCCCAACTC
AGTTAAACAATACCTTCGTAAAGATGACAGATCTCCGTTATGGGGAAAATCCGCATCAAAGCGGTGCGTTCTACCGCGAT
GTACATCCGCAACCTGGGACGCTGGCCACCTTCAAGCAACTCCAAGGCAAGAGACTCAGCTACAACAATCTGGTCGATGC
CGACGCCGCATGGGAATGCGTACGTCAATTCGAAGCGCCGGCCTGCGTCATCGTCAAACATGCCAACCCTTGTGGCGTCG
CAGTCGGGACGGCGTGCAGTGATGCTTATGAAGCAGCGTATGCCACTGATCCGACGAGTGCATTTGGCGGCATTATTGCT
TTTAACCGCACGTTAGATGCGGTCACTATGCAAAGCATTCTAGACCGTCAATTTGTCGAGGTATTGATTGCGCCGTATTA
CGATGCAGACGCCCTCGCCTATGCGACCAAGAAGGCCAATGTGCGCGTGCTGCGTATCCCCAGCACTGGCGCGATGAATC
GCTATGATTTCAAACGGATCGGCTCTGGACTATTGGTGCAAAGCGCCGACAGTCTGAACATCCATTCGGGTGCACTGAAG
GTGGTCACTCAGCTTGCTCCCACCTATGCACAACAACGTGATCTGTTGTTTGCATGGCATGTCGCCAAGTATGTCAAGTC
AAATGCCATTGTTTACGCCAAGGATAATCGCACGATTGGTATTGGCGCCGGACAAATGAGTCGCGTGTATTCAGCGCGTA
TCGCTGGTATCAAGGCAGCCGATGCACATTTGGCTGTCACAGGCTCAGTGATGGCCAGCGATGCGTTCTTTCCATTCCGC
GATGGCATTGATGCCGCTGCCGCGACTGGAATCAAGGCAGTGATTCAACCGGGCGGTTCGATGCGCGATAACGAGGTGAT
CGCGGCGGCTGATGAACACGGCATTGCCATGGTCTTCACAGGGATACGGCATTTCCGGCATTGA

Upstream 100 bases:

>100_bases
CCGGTGCGTTGAGAGCAATGACCCAGGCATTCCAGGATCCCATCCTGACACTGGGAAGACCTACAATGCTACCTCACTCC
ACTCCCGGCGGTTTTGCTTT

Downstream 100 bases:

>100_bases
ACGGAAATGGGTTCCGCGGATGCTTGACAATGTGTTTTTGGCTTGAATAGCTGCATATGACACAAGTACTGCAATGCATC
CGATGCAAATAGCAAAACCA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MASDFLPVHRALLSVSDKTGLVELARVLLAYNIELLSTGGTATIIREAGLPVQDVADLTDFPEMMDGRVKTLHPVVHGGL
LGRAGIDDAVMAKHGIAPIDLLIVNLYPFEQITAKKDCTLADAVDTIDIGGPAMLRSAAKNFARVAVATSPDQYPDLLAE
LQAHHGQLSAEKRFALAVAAFNHVAQYDAAISNYLSSVSDMHTTLPLRHEFPTQLNNTFVKMTDLRYGENPHQSGAFYRD
VHPQPGTLATFKQLQGKRLSYNNLVDADAAWECVRQFEAPACVIVKHANPCGVAVGTACSDAYEAAYATDPTSAFGGIIA
FNRTLDAVTMQSILDRQFVEVLIAPYYDADALAYATKKANVRVLRIPSTGAMNRYDFKRIGSGLLVQSADSLNIHSGALK
VVTQLAPTYAQQRDLLFAWHVAKYVKSNAIVYAKDNRTIGIGAGQMSRVYSARIAGIKAADAHLAVTGSVMASDAFFPFR
DGIDAAAATGIKAVIQPGGSMRDNEVIAAADEHGIAMVFTGIRHFRH

Sequences:

>Translated_527_residues
MASDFLPVHRALLSVSDKTGLVELARVLLAYNIELLSTGGTATIIREAGLPVQDVADLTDFPEMMDGRVKTLHPVVHGGL
LGRAGIDDAVMAKHGIAPIDLLIVNLYPFEQITAKKDCTLADAVDTIDIGGPAMLRSAAKNFARVAVATSPDQYPDLLAE
LQAHHGQLSAEKRFALAVAAFNHVAQYDAAISNYLSSVSDMHTTLPLRHEFPTQLNNTFVKMTDLRYGENPHQSGAFYRD
VHPQPGTLATFKQLQGKRLSYNNLVDADAAWECVRQFEAPACVIVKHANPCGVAVGTACSDAYEAAYATDPTSAFGGIIA
FNRTLDAVTMQSILDRQFVEVLIAPYYDADALAYATKKANVRVLRIPSTGAMNRYDFKRIGSGLLVQSADSLNIHSGALK
VVTQLAPTYAQQRDLLFAWHVAKYVKSNAIVYAKDNRTIGIGAGQMSRVYSARIAGIKAADAHLAVTGSVMASDAFFPFR
DGIDAAAATGIKAVIQPGGSMRDNEVIAAADEHGIAMVFTGIRHFRH
>Mature_526_residues
ASDFLPVHRALLSVSDKTGLVELARVLLAYNIELLSTGGTATIIREAGLPVQDVADLTDFPEMMDGRVKTLHPVVHGGLL
GRAGIDDAVMAKHGIAPIDLLIVNLYPFEQITAKKDCTLADAVDTIDIGGPAMLRSAAKNFARVAVATSPDQYPDLLAEL
QAHHGQLSAEKRFALAVAAFNHVAQYDAAISNYLSSVSDMHTTLPLRHEFPTQLNNTFVKMTDLRYGENPHQSGAFYRDV
HPQPGTLATFKQLQGKRLSYNNLVDADAAWECVRQFEAPACVIVKHANPCGVAVGTACSDAYEAAYATDPTSAFGGIIAF
NRTLDAVTMQSILDRQFVEVLIAPYYDADALAYATKKANVRVLRIPSTGAMNRYDFKRIGSGLLVQSADSLNIHSGALKV
VTQLAPTYAQQRDLLFAWHVAKYVKSNAIVYAKDNRTIGIGAGQMSRVYSARIAGIKAADAHLAVTGSVMASDAFFPFRD
GIDAAAATGIKAVIQPGGSMRDNEVIAAADEHGIAMVFTGIRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=484, Percent_Identity=37.8099173553719, Blast_Score=265, Evalue=8e-71,
Organism=Escherichia coli, GI1790439, Length=530, Percent_Identity=60, Blast_Score=632, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=612, Percent_Identity=32.1895424836601, Blast_Score=281, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI71985574, Length=353, Percent_Identity=26.0623229461756, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=61, Percent_Identity=54.0983606557377, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323768, Length=477, Percent_Identity=37.3165618448637, Blast_Score=273, Evalue=5e-74,
Organism=Saccharomyces cerevisiae, GI6323056, Length=485, Percent_Identity=35.8762886597938, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24649832, Length=609, Percent_Identity=35.7963875205254, Blast_Score=305, Evalue=5e-83,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56725; Mature: 56594

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDFLPVHRALLSVSDKTGLVELARVLLAYNIELLSTGGTATIIREAGLPVQDVADLTD
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEECCCCEEEEECCCCCHHHHHHHHC
FPEMMDGRVKTLHPVVHGGLLGRAGIDDAVMAKHGIAPIDLLIVNLYPFEQITAKKDCTL
CHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEEEEECCCHHHHCCCCCCCH
ADAVDTIDIGGPAMLRSAAKNFARVAVATSPDQYPDLLAELQAHHGQLSAEKRFALAVAA
HHCCCEECCCCHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FNHVAQYDAAISNYLSSVSDMHTTLPLRHEFPTQLNNTFVKMTDLRYGENPHQSGAFYRD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCCEEEE
VHPQPGTLATFKQLQGKRLSYNNLVDADAAWECVRQFEAPACVIVKHANPCGVAVGTACS
CCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEECCHHH
DAYEAAYATDPTSAFGGIIAFNRTLDAVTMQSILDRQFVEVLIAPYYDADALAYATKKAN
HHHHHHCCCCCCHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCC
VRVLRIPSTGAMNRYDFKRIGSGLLVQSADSLNIHSGALKVVTQLAPTYAQQRDLLFAWH
EEEEECCCCCCCCCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
VAKYVKSNAIVYAKDNRTIGIGAGQMSRVYSARIAGIKAADAHLAVTGSVMASDAFFPFR
HHHHHHCCEEEEEECCCEEEECCCHHHHHHHHHHCCCEECCCEEEEECCHHCCCCCCCCC
DGIDAAAATGIKAVIQPGGSMRDNEVIAAADEHGIAMVFTGIRHFRH
CCCCHHHHHCCHHEECCCCCCCCCCEEEEECCCCEEHEEHHHHHHCC
>Mature Secondary Structure 
ASDFLPVHRALLSVSDKTGLVELARVLLAYNIELLSTGGTATIIREAGLPVQDVADLTD
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEECCCCEEEEECCCCCHHHHHHHHC
FPEMMDGRVKTLHPVVHGGLLGRAGIDDAVMAKHGIAPIDLLIVNLYPFEQITAKKDCTL
CHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEEEEECCCHHHHCCCCCCCH
ADAVDTIDIGGPAMLRSAAKNFARVAVATSPDQYPDLLAELQAHHGQLSAEKRFALAVAA
HHCCCEECCCCHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FNHVAQYDAAISNYLSSVSDMHTTLPLRHEFPTQLNNTFVKMTDLRYGENPHQSGAFYRD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCCEEEE
VHPQPGTLATFKQLQGKRLSYNNLVDADAAWECVRQFEAPACVIVKHANPCGVAVGTACS
CCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEECCHHH
DAYEAAYATDPTSAFGGIIAFNRTLDAVTMQSILDRQFVEVLIAPYYDADALAYATKKAN
HHHHHHCCCCCCHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCC
VRVLRIPSTGAMNRYDFKRIGSGLLVQSADSLNIHSGALKVVTQLAPTYAQQRDLLFAWH
EEEEECCCCCCCCCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
VAKYVKSNAIVYAKDNRTIGIGAGQMSRVYSARIAGIKAADAHLAVTGSVMASDAFFPFR
HHHHHHCCEEEEEECCCEEEECCCHHHHHHHHHHCCCEECCCEEEEECCHHCCCCCCCCC
DGIDAAAATGIKAVIQPGGSMRDNEVIAAADEHGIAMVFTGIRHFRH
CCCCHHHHHCCHHEECCCCCCCCCCEEEEECCCCEEHEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA