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The map label for this gene is folE

Identifier: 182681421

GI number: 182681421

Start: 1024313

End: 1024924

Strand: Reverse

Name: folE

Synonym: XfasM23_0871

Alternate gene names: 182681421

Gene position: 1024924-1024313 (Counterclockwise)

Preceding gene: 182681424

Following gene: 182681420

Centisome position: 40.42

GC content: 51.63

Gene sequence:

>612_bases
ATGGACCATTCCAAACAACAAAACGCATCCATCACCCAAGCTCAGGCAGAAGAGGCCGTACGCACACTTCTACGTTGGGC
CGGCGAAGATCCCACTCGTGAAGGCTTGCTCGACACACCACGCCGTGTCGTTGAAGCATACGGAGATTGGTTCAGCGGTT
ACCGCGAAGATCCTCATGACTACCTGCAACGCACTTTCGAGGAAATCTCTTGCTACGACGAGATGATCGTACTACGTAAC
ATTACCTATGAAAGCCACTGCGAACATCACATGGCACCCATCATCGGTAAAGTTCACGTTGGCTACTTGCCAAACGGCAA
AGTCGTCGGCATCAGCAAATTAGCACGCGTTGTTGAATCCTATGCGCGCCGCTTCCAGATACAGGAAAAAATGACTGCGC
AAATTGCAGCATGTATCCAAGACACATTGACCCCACGTGGCGTCGGTGTCGTGATTGAAGGTGCACACGCATGTATGACT
ACCCGAGGTATACACAAGCGTGGTGTATCAATGGTCACCTCAAAGATGCTCGGCACCTTCCGCGAAGACGCACGCACCCG
CGCCGAGTTCTTACAGTTCATCGAAGTAGGTACCAACGTGATCGACTTATGA

Upstream 100 bases:

>100_bases
TCTAAAATTCATTATCAGCCGTATTTAACATGTACCCCCCTTACCTTTCAAAGAGAGTGAGTTCAGGTACCATGCATGTT
ACACATACTGTTTGCTCATC

Downstream 100 bases:

>100_bases
ATGACTGCGGCCACATCGGCAGCTACCGTTCCCTTCGCTACCAAGCACTCTAGAAGCTGTGGTTTCTGCCGATCAGGTTC
CTAAAATCATCGGATTTTTT

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MDHSKQQNASITQAQAEEAVRTLLRWAGEDPTREGLLDTPRRVVEAYGDWFSGYREDPHDYLQRTFEEISCYDEMIVLRN
ITYESHCEHHMAPIIGKVHVGYLPNGKVVGISKLARVVESYARRFQIQEKMTAQIAACIQDTLTPRGVGVVIEGAHACMT
TRGIHKRGVSMVTSKMLGTFREDARTRAEFLQFIEVGTNVIDL

Sequences:

>Translated_203_residues
MDHSKQQNASITQAQAEEAVRTLLRWAGEDPTREGLLDTPRRVVEAYGDWFSGYREDPHDYLQRTFEEISCYDEMIVLRN
ITYESHCEHHMAPIIGKVHVGYLPNGKVVGISKLARVVESYARRFQIQEKMTAQIAACIQDTLTPRGVGVVIEGAHACMT
TRGIHKRGVSMVTSKMLGTFREDARTRAEFLQFIEVGTNVIDL
>Mature_203_residues
MDHSKQQNASITQAQAEEAVRTLLRWAGEDPTREGLLDTPRRVVEAYGDWFSGYREDPHDYLQRTFEEISCYDEMIVLRN
ITYESHCEHHMAPIIGKVHVGYLPNGKVVGISKLARVVESYARRFQIQEKMTAQIAACIQDTLTPRGVGVVIEGAHACMT
TRGIHKRGVSMVTSKMLGTFREDARTRAEFLQFIEVGTNVIDL

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=177, Percent_Identity=50.2824858757062, Blast_Score=183, Evalue=8e-47,
Organism=Homo sapiens, GI4503949, Length=177, Percent_Identity=50.2824858757062, Blast_Score=183, Evalue=8e-47,
Organism=Homo sapiens, GI66932970, Length=147, Percent_Identity=46.2585034013605, Blast_Score=139, Evalue=1e-33,
Organism=Homo sapiens, GI66932972, Length=140, Percent_Identity=48.5714285714286, Blast_Score=137, Evalue=5e-33,
Organism=Escherichia coli, GI1788476, Length=175, Percent_Identity=36, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=46.3276836158192, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6321706, Length=178, Percent_Identity=51.123595505618, Blast_Score=180, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24656782, Length=175, Percent_Identity=46.8571428571429, Blast_Score=156, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24656796, Length=195, Percent_Identity=43.5897435897436, Blast_Score=156, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24656791, Length=195, Percent_Identity=43.5897435897436, Blast_Score=156, Evalue=1e-38,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_XYLF2 (B2I4L2)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829581.1
- ProteinModelPortal:   B2I4L2
- SMR:   B2I4L2
- GeneID:   6202208
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0871
- HOGENOM:   HBG370191
- OMA:   IQERLTT
- ProtClustDB:   PRK09347
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 22983; Mature: 22983

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHSKQQNASITQAQAEEAVRTLLRWAGEDPTREGLLDTPRRVVEAYGDWFSGYREDPHD
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
YLQRTFEEISCYDEMIVLRNITYESHCEHHMAPIIGKVHVGYLPNGKVVGISKLARVVES
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCEECHHHHHHHHHH
YARRFQIQEKMTAQIAACIQDTLTPRGVGVVIEGAHACMTTRGIHKRGVSMVTSKMLGTF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
REDARTRAEFLQFIEVGTNVIDL
HHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MDHSKQQNASITQAQAEEAVRTLLRWAGEDPTREGLLDTPRRVVEAYGDWFSGYREDPHD
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
YLQRTFEEISCYDEMIVLRNITYESHCEHHMAPIIGKVHVGYLPNGKVVGISKLARVVES
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCEECHHHHHHHHHH
YARRFQIQEKMTAQIAACIQDTLTPRGVGVVIEGAHACMTTRGIHKRGVSMVTSKMLGTF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
REDARTRAEFLQFIEVGTNVIDL
HHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA