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The map label for this gene is bioH

Identifier: 182681184

GI number: 182681184

Start: 740776

End: 741543

Strand: Reverse

Name: bioH

Synonym: XfasM23_0627

Alternate gene names: 182681184

Gene position: 741543-740776 (Counterclockwise)

Preceding gene: 182681185

Following gene: 182681182

Centisome position: 29.24

GC content: 56.25

Gene sequence:

>768_bases
ATGTATATCGAAGTTACCGGATATGGTCCGGCACTAGTTTTGATCCACGGATGGGCAATGCACAGCGGTGTATTCGCACC
GCTGGTCGAACAACTCAGGCCACACCATACACTCTATTTGGTCGACCTCCCCGGACACGGCTACAACCACACCACTCCCA
CGCCACTGGCCCTACCGCAGGTGGTTCACGCAATCGCAGCGGCGACCCCTCCAGCAGTGTGGTTGGGCTGGTCACTTGGT
GGATTATTCGCACTTCACGCCGCAGCGACCCTGCCCCAAGTACGTGGCCTCATCATGCTGGCCGCTACCCCATGCTTCGT
ACGGCGTGAAGATTGGCCACACGCCGTTGAAGTGAGCATATTGACCCAATTCGCCCAAGATCTGAAACAAAACTATACAG
AGACCATCAACCGCTTTCTTGCACTGGACACACTTGGATCAACACACGCACAAAGCGAACTGCGCCAATTACGGAAAATT
TTAAATGCACGCCACACACCCAACACCGCAACACTCCAAGCAGGTCTGGAGCTGCTCGCACATACCGACTTACGTCGCGC
CCTGATTGACCTCACACCTCCAAGCCTATGGATCGCCGGGCAACGCGATCGACTGGTTCCCGCTGCGAGCATCCAGGCAG
CAACGGTACTGGCACCGAGTGACCAGACCGAATTACTCACCATCACCGGCGGTGGCCACGCCCCGTTCCTCAGTCATGCC
AATCAAATGACGGCAGCATTACAGCACTTCATTGCCACTCTCCCCTAA

Upstream 100 bases:

>100_bases
AAGCACGACACTGGCCCCCTGACCATTCAAGCACCTTAGCGCCGATAACTGATCGACCAATAGATCACCCAGATTCCTCC
TATCAGCAACACATCTAACA

Downstream 100 bases:

>100_bases
CCCCTAAATCACCTCGATTCCAATTTACACATCACGGCCCTCCAACCTGGATGAACTACAGTCGTACCATCGTCCCGCGT
CCCCAAAGAGCCACCAAACA

Product: bioH protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MYIEVTGYGPALVLIHGWAMHSGVFAPLVEQLRPHHTLYLVDLPGHGYNHTTPTPLALPQVVHAIAAATPPAVWLGWSLG
GLFALHAAATLPQVRGLIMLAATPCFVRREDWPHAVEVSILTQFAQDLKQNYTETINRFLALDTLGSTHAQSELRQLRKI
LNARHTPNTATLQAGLELLAHTDLRRALIDLTPPSLWIAGQRDRLVPAASIQAATVLAPSDQTELLTITGGGHAPFLSHA
NQMTAALQHFIATLP

Sequences:

>Translated_255_residues
MYIEVTGYGPALVLIHGWAMHSGVFAPLVEQLRPHHTLYLVDLPGHGYNHTTPTPLALPQVVHAIAAATPPAVWLGWSLG
GLFALHAAATLPQVRGLIMLAATPCFVRREDWPHAVEVSILTQFAQDLKQNYTETINRFLALDTLGSTHAQSELRQLRKI
LNARHTPNTATLQAGLELLAHTDLRRALIDLTPPSLWIAGQRDRLVPAASIQAATVLAPSDQTELLTITGGGHAPFLSHA
NQMTAALQHFIATLP
>Mature_255_residues
MYIEVTGYGPALVLIHGWAMHSGVFAPLVEQLRPHHTLYLVDLPGHGYNHTTPTPLALPQVVHAIAAATPPAVWLGWSLG
GLFALHAAATLPQVRGLIMLAATPCFVRREDWPHAVEVSILTQFAQDLKQNYTETINRFLALDTLGSTHAQSELRQLRKI
LNARHTPNTATLQAGLELLAHTDLRRALIDLTPPSLWIAGQRDRLVPAASIQAATVLAPSDQTELLTITGGGHAPFLSHA
NQMTAALQHFIATLP

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family

Homologues:

Organism=Escherichia coli, GI1789817, Length=242, Percent_Identity=37.603305785124, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOH_XYLF2 (B2I9H6)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829344.1
- ProteinModelPortal:   B2I9H6
- GeneID:   6203659
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0627
- HOGENOM:   HBG748003
- OMA:   CTIASSP
- ProtClustDB:   CLSK446229
- GO:   GO:0005737
- HAMAP:   MF_01260
- InterPro:   IPR000073
- InterPro:   IPR010076
- TIGRFAMs:   TIGR01738

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.1.1.1

Molecular weight: Translated: 27658; Mature: 27658

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: ACT_SITE 78-78 ACT_SITE 203-203 ACT_SITE 233-233

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIEVTGYGPALVLIHGWAMHSGVFAPLVEQLRPHHTLYLVDLPGHGYNHTTPTPLALPQ
CEEEEECCCCEEEEEECHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHH
VVHAIAAATPPAVWLGWSLGGLFALHAAATLPQVRGLIMLAATPCFVRREDWPHAVEVSI
HHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEECCCCCCHHHHHH
LTQFAQDLKQNYTETINRFLALDTLGSTHAQSELRQLRKILNARHTPNTATLQAGLELLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
HTDLRRALIDLTPPSLWIAGQRDRLVPAASIQAATVLAPSDQTELLTITGGGHAPFLSHA
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHH
NQMTAALQHFIATLP
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MYIEVTGYGPALVLIHGWAMHSGVFAPLVEQLRPHHTLYLVDLPGHGYNHTTPTPLALPQ
CEEEEECCCCEEEEEECHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHH
VVHAIAAATPPAVWLGWSLGGLFALHAAATLPQVRGLIMLAATPCFVRREDWPHAVEVSI
HHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEECCCCCCHHHHHH
LTQFAQDLKQNYTETINRFLALDTLGSTHAQSELRQLRKILNARHTPNTATLQAGLELLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
HTDLRRALIDLTPPSLWIAGQRDRLVPAASIQAATVLAPSDQTELLTITGGGHAPFLSHA
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHH
NQMTAALQHFIATLP
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA