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The map label for this gene is bioF

Identifier: 182681185

GI number: 182681185

Start: 741700

End: 742905

Strand: Reverse

Name: bioF

Synonym: XfasM23_0628

Alternate gene names: 182681185

Gene position: 742905-741700 (Counterclockwise)

Preceding gene: 182681188

Following gene: 182681184

Centisome position: 29.3

GC content: 57.46

Gene sequence:

>1206_bases
GTGACCCGACCCGATTTGAATGAACGCATACTGTCACTACGCAAACTGCGTCTAGCACAATGCCGCACACGCACACGACG
CACAATCGAAAGGCGTAACGGCGTTCGGCTCGAAATCAACGGAAGCTGGCTGGTCGAATTCTGTTCCAACGACTACCTAG
GACTCGCGCAACACTTCGAAATCATCGCAGCACTACAGGATGCAGCCGCCCGCAATGGCATCGGTGCCACTGCTTCGCAT
CTGATCTGCGGCCACCACGCAATACATAAGGCACTGGAATATGAATTAGCCGAATGGTTGGGCTACCCACGCGCATTACT
GTTCGGTAGCGGCTTCACCGCCAACCTGGCAGTGCAACAAGCTCTGCTCACCAAAGAAAACGACATCTGCGTTCAAGACC
GACTCAACCATGCCAGCCTGATAGATGCTACCCGTCTGGCAGGCTGCCGCCTACGCCGCTACCCGCACCTTGATGTGGAT
GGAGCGGCACATCAACTCAAGAACGCACCCGAAGGTGCAGCAATGCTAGCAACCGATGGCATCTTTAGCATGGATGGCGA
CATCGCTCCATTACGCGCCCTCAGCCTGGTCGCACGCACCCAACAAGCACTGATGTATGTCGATGACGCACACGGCATCG
GAGTGACCGGACCCCAAGGGCGTGGCTGTATCGCAGCTGCTTGGCTCAGCGTAGAGGAAGTCCCCTTGCAATTAGTGACC
CTCAGCAAAGCCCTAGGTGGCTACGGTGCGGCCGTACTCGGCTCTGCCACACTGATCCAACACTTGGCCGAAACCGCACG
CCCCTACATATACACCACTGCACTGCCTCCTGCCCAAGCCGCCGCCGCACTGACAGCAATCCGTATTGCACGGCGTGACG
AATGGCGCCGCCAGCGCCTACAGGAACTTGTGGAGCGTTTCCGCGAGAATAGCCGCCGTCACGGCCTGGAGATCATGGAT
TCAGAGACACCTATCCAACCCTTGCAATGTGGAGATGAAACCACAACGATGGCGATGTCCGCGGCATTGGAACGCGAAGG
GTTCCTAGTCAACGCAATCCGGCCACCCACCGTACCCGAGGGGAAATCACGGTTGCGAGTGACCTTGTCCGCACTGCATA
CCACTGAACAAATCGATACATTAGTGCAAGCGCTTGCCCGCTCCCGCGATGCCCTCGCGACCGAAGCCGCACCTGTAAAA
GTATAA

Upstream 100 bases:

>100_bases
GACCACCAAGCTTGACAAACTCCGGAACACCCACTGACTGGGCGACATGGCAGTTGACGCGCTAGGCTAACGGACTCAAC
GCTCACATAGGGATGCCGGC

Downstream 100 bases:

>100_bases
ATACGCGATATCAAGGCAGGGGGGGGGGCTCCCCCACATCTCAGCGCAAGACAAAAAAGCACGACACTGGCCCCCTGACC
ATTCAAGCACCTTAGCGCCG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTRPDLNERILSLRKLRLAQCRTRTRRTIERRNGVRLEINGSWLVEFCSNDYLGLAQHFEIIAALQDAAARNGIGATASH
LICGHHAIHKALEYELAEWLGYPRALLFGSGFTANLAVQQALLTKENDICVQDRLNHASLIDATRLAGCRLRRYPHLDVD
GAAHQLKNAPEGAAMLATDGIFSMDGDIAPLRALSLVARTQQALMYVDDAHGIGVTGPQGRGCIAAAWLSVEEVPLQLVT
LSKALGGYGAAVLGSATLIQHLAETARPYIYTTALPPAQAAAALTAIRIARRDEWRRQRLQELVERFRENSRRHGLEIMD
SETPIQPLQCGDETTTMAMSAALEREGFLVNAIRPPTVPEGKSRLRVTLSALHTTEQIDTLVQALARSRDALATEAAPVK
V

Sequences:

>Translated_401_residues
MTRPDLNERILSLRKLRLAQCRTRTRRTIERRNGVRLEINGSWLVEFCSNDYLGLAQHFEIIAALQDAAARNGIGATASH
LICGHHAIHKALEYELAEWLGYPRALLFGSGFTANLAVQQALLTKENDICVQDRLNHASLIDATRLAGCRLRRYPHLDVD
GAAHQLKNAPEGAAMLATDGIFSMDGDIAPLRALSLVARTQQALMYVDDAHGIGVTGPQGRGCIAAAWLSVEEVPLQLVT
LSKALGGYGAAVLGSATLIQHLAETARPYIYTTALPPAQAAAALTAIRIARRDEWRRQRLQELVERFRENSRRHGLEIMD
SETPIQPLQCGDETTTMAMSAALEREGFLVNAIRPPTVPEGKSRLRVTLSALHTTEQIDTLVQALARSRDALATEAAPVK
V
>Mature_400_residues
TRPDLNERILSLRKLRLAQCRTRTRRTIERRNGVRLEINGSWLVEFCSNDYLGLAQHFEIIAALQDAAARNGIGATASHL
ICGHHAIHKALEYELAEWLGYPRALLFGSGFTANLAVQQALLTKENDICVQDRLNHASLIDATRLAGCRLRRYPHLDVDG
AAHQLKNAPEGAAMLATDGIFSMDGDIAPLRALSLVARTQQALMYVDDAHGIGVTGPQGRGCIAAAWLSVEEVPLQLVTL
SKALGGYGAAVLGSATLIQHLAETARPYIYTTALPPAQAAAALTAIRIARRDEWRRQRLQELVERFRENSRRHGLEIMDS
ETPIQPLQCGDETTTMAMSAALEREGFLVNAIRPPTVPEGKSRLRVTLSALHTTEQIDTLVQALARSRDALATEAAPVKV

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=366, Percent_Identity=36.3387978142077, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI284448556, Length=346, Percent_Identity=37.2832369942197, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI83977444, Length=347, Percent_Identity=31.700288184438, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI83977442, Length=347, Percent_Identity=31.700288184438, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI83977440, Length=347, Percent_Identity=31.700288184438, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4502025, Length=358, Percent_Identity=31.0055865921788, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI40316939, Length=358, Percent_Identity=31.0055865921788, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI4758668, Length=370, Percent_Identity=28.9189189189189, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI119220554, Length=367, Percent_Identity=26.7029972752044, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI5454084, Length=266, Percent_Identity=23.6842105263158, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786993, Length=354, Percent_Identity=45.7627118644068, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1790046, Length=353, Percent_Identity=36.5439093484419, Blast_Score=218, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI71994529, Length=369, Percent_Identity=36.0433604336043, Blast_Score=234, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI32566772, Length=365, Percent_Identity=27.9452054794521, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17560912, Length=383, Percent_Identity=25.8485639686684, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560914, Length=386, Percent_Identity=26.4248704663212, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71982625, Length=278, Percent_Identity=23.021582733813, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71982617, Length=278, Percent_Identity=23.021582733813, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320438, Length=363, Percent_Identity=29.2011019283747, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320267, Length=414, Percent_Identity=24.1545893719807, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323954, Length=294, Percent_Identity=24.1496598639456, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24662918, Length=365, Percent_Identity=36.4383561643836, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI17137420, Length=351, Percent_Identity=33.048433048433, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17136286, Length=364, Percent_Identity=26.0989010989011, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24653280, Length=389, Percent_Identity=21.8508997429306, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653276, Length=389, Percent_Identity=21.8508997429306, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653278, Length=389, Percent_Identity=21.8508997429306, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_XYLF2 (B2I9H7)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829345.1
- ProteinModelPortal:   B2I9H7
- GeneID:   6203572
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0628
- HOGENOM:   HBG649839
- OMA:   ATVMAMS
- ProtClustDB:   PRK05958
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 43891; Mature: 43760

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 24-24 BINDING 137-137 BINDING 183-183 BINDING 357-357

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPDLNERILSLRKLRLAQCRTRTRRTIERRNGVRLEINGSWLVEFCSNDYLGLAQHFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCHHHHHHHH
IIAALQDAAARNGIGATASHLICGHHAIHKALEYELAEWLGYPRALLFGSGFTANLAVQQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHH
ALLTKENDICVQDRLNHASLIDATRLAGCRLRRYPHLDVDGAAHQLKNAPEGAAMLATDG
HHHHCCCCEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCEEEEECC
IFSMDGDIAPLRALSLVARTQQALMYVDDAHGIGVTGPQGRGCIAAAWLSVEEVPLQLVT
CEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LSKALGGYGAAVLGSATLIQHLAETARPYIYTTALPPAQAAAALTAIRIARRDEWRRQRL
HHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
QELVERFRENSRRHGLEIMDSETPIQPLQCGDETTTMAMSAALEREGFLVNAIRPPTVPE
HHHHHHHHHHHHHCCCEEECCCCCCCHHHCCCHHHHHHHHHHHHHCCEEEECCCCCCCCC
GKSRLRVTLSALHTTEQIDTLVQALARSRDALATEAAPVKV
CCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TRPDLNERILSLRKLRLAQCRTRTRRTIERRNGVRLEINGSWLVEFCSNDYLGLAQHFE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCHHHHHHHH
IIAALQDAAARNGIGATASHLICGHHAIHKALEYELAEWLGYPRALLFGSGFTANLAVQQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHH
ALLTKENDICVQDRLNHASLIDATRLAGCRLRRYPHLDVDGAAHQLKNAPEGAAMLATDG
HHHHCCCCEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCEEEEECC
IFSMDGDIAPLRALSLVARTQQALMYVDDAHGIGVTGPQGRGCIAAAWLSVEEVPLQLVT
CEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LSKALGGYGAAVLGSATLIQHLAETARPYIYTTALPPAQAAAALTAIRIARRDEWRRQRL
HHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
QELVERFRENSRRHGLEIMDSETPIQPLQCGDETTTMAMSAALEREGFLVNAIRPPTVPE
HHHHHHHHHHHHHCCCEEECCCCCCCHHHCCCHHHHHHHHHHHHHCCEEEECCCCCCCCC
GKSRLRVTLSALHTTEQIDTLVQALARSRDALATEAAPVKV
CCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA