Click here to switch to the map view.

The map label for this gene is htrB [H]

Identifier: 182681180

GI number: 182681180

Start: 733200

End: 734147

Strand: Reverse

Name: htrB [H]

Synonym: XfasM23_0622

Alternate gene names: 182681180

Gene position: 734147-733200 (Counterclockwise)

Preceding gene: 182681182

Following gene: 182681171

Centisome position: 28.95

GC content: 51.79

Gene sequence:

>948_bases
ATGAACGCAAACTCCAAAATCCGCTGGTTATATCGGGTCGCATCATTGGTAGGGCACATCCCCTGGCCACTCCTCAAACG
ACTGTCAGATCTGATCGCTTACTGCTGGCTTTGTTTGAACGCCCGGGAAAGTCGTGTGACACAACGCAATTTGGAACTGA
CCTATCCAGAACTATCTCCCCAGGAGCACACGCGCCTACATCACCAAATCATTTATTCCACAGTGCGCCAAGCGTTCGAA
ATGCTACATATCTGGACCCACCCAGAGACAGAAAACTTGGCGCGACTACGTGAATACCACGGCGTAGATTTGTACAGTGC
AGCCCTAGCCCGCAACCGAGGGATGATTATCGTTGTCCCCCATTTTGGCAACTGGGAATTACTGAAACAGTACCTGTCCG
ATCTTGCGCCGCTCACGCTCATGTACCGTCCAGCGAATTCGGCCGTGTTAGATGGCTTTCTACAACGAGTACGTGGTGGC
AACAATGTCCATCAAGTTCGTGCAGAAGGGATGGCAGTACGGCATCTTTTCAAAGTGCTCAAAAATGGCGGCACAATCGG
AATCCTTCCAGATCAACAACCGAAGATCGGCGAAGGTGTATTTGCACCATTCTTCGGCATCGAAGCATTGACCATGACTC
TTATACAGCGTTTAGCGGAACGGACCGGCGCAACCGTGCTATACGCCTGGTGCGAGCGAATTAGTCCTCATCTAGAGTTC
GCCTTGCATATGGAACACGCCGACCCAGCGGTGGCGGATCCAGATCCACTGATCGCGGCGACCGCACTTAATGCAGGGAT
CGAACGTATCGCCAGACGCGACCCAACTCAGTATCAGTGGACTTACAAGCGCTACAGCTTGCGCCCACCGGATAGCGGCG
AACACAACCCCTATGAAATAAAACACCAATCACACTCAATAGCGAAAACACTCAAGCATCCTGATTGA

Upstream 100 bases:

>100_bases
CAGAAGACATGAATCACACACCAGTACACCACAGAACGATATGACTGATCGCAACCTAATCTCCAACTCGTGAAGATAGC
GTCCTTATAAAATTGCATAA

Downstream 100 bases:

>100_bases
TGATGCACTTATCAAAGCAAACCCACTGAATTAAATAACAAACGTAGCGTTTGAAACGACTTGATTGCACAAACTGAGCG
GAAGATCACCCCGCACAAAT

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNANSKIRWLYRVASLVGHIPWPLLKRLSDLIAYCWLCLNARESRVTQRNLELTYPELSPQEHTRLHHQIIYSTVRQAFE
MLHIWTHPETENLARLREYHGVDLYSAALARNRGMIIVVPHFGNWELLKQYLSDLAPLTLMYRPANSAVLDGFLQRVRGG
NNVHQVRAEGMAVRHLFKVLKNGGTIGILPDQQPKIGEGVFAPFFGIEALTMTLIQRLAERTGATVLYAWCERISPHLEF
ALHMEHADPAVADPDPLIAATALNAGIERIARRDPTQYQWTYKRYSLRPPDSGEHNPYEIKHQSHSIAKTLKHPD

Sequences:

>Translated_315_residues
MNANSKIRWLYRVASLVGHIPWPLLKRLSDLIAYCWLCLNARESRVTQRNLELTYPELSPQEHTRLHHQIIYSTVRQAFE
MLHIWTHPETENLARLREYHGVDLYSAALARNRGMIIVVPHFGNWELLKQYLSDLAPLTLMYRPANSAVLDGFLQRVRGG
NNVHQVRAEGMAVRHLFKVLKNGGTIGILPDQQPKIGEGVFAPFFGIEALTMTLIQRLAERTGATVLYAWCERISPHLEF
ALHMEHADPAVADPDPLIAATALNAGIERIARRDPTQYQWTYKRYSLRPPDSGEHNPYEIKHQSHSIAKTLKHPD
>Mature_315_residues
MNANSKIRWLYRVASLVGHIPWPLLKRLSDLIAYCWLCLNARESRVTQRNLELTYPELSPQEHTRLHHQIIYSTVRQAFE
MLHIWTHPETENLARLREYHGVDLYSAALARNRGMIIVVPHFGNWELLKQYLSDLAPLTLMYRPANSAVLDGFLQRVRGG
NNVHQVRAEGMAVRHLFKVLKNGGTIGILPDQQPKIGEGVFAPFFGIEALTMTLIQRLAERTGATVLYAWCERISPHLEF
ALHMEHADPAVADPDPLIAATALNAGIERIARRDPTQYQWTYKRYSLRPPDSGEHNPYEIKHQSHSIAKTLKHPD

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=278, Percent_Identity=26.6187050359712, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI87082094, Length=287, Percent_Identity=26.4808362369338, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 36081; Mature: 36081

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANSKIRWLYRVASLVGHIPWPLLKRLSDLIAYCWLCLNARESRVTQRNLELTYPELSP
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCCC
QEHTRLHHQIIYSTVRQAFEMLHIWTHPETENLARLREYHGVDLYSAALARNRGMIIVVP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEE
HFGNWELLKQYLSDLAPLTLMYRPANSAVLDGFLQRVRGGNNVHQVRAEGMAVRHLFKVL
CCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KNGGTIGILPDQQPKIGEGVFAPFFGIEALTMTLIQRLAERTGATVLYAWCERISPHLEF
HCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHE
ALHMEHADPAVADPDPLIAATALNAGIERIARRDPTQYQWTYKRYSLRPPDSGEHNPYEI
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEE
KHQSHSIAKTLKHPD
CCCHHHHHHHHCCCC
>Mature Secondary Structure
MNANSKIRWLYRVASLVGHIPWPLLKRLSDLIAYCWLCLNARESRVTQRNLELTYPELSP
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCCC
QEHTRLHHQIIYSTVRQAFEMLHIWTHPETENLARLREYHGVDLYSAALARNRGMIIVVP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEE
HFGNWELLKQYLSDLAPLTLMYRPANSAVLDGFLQRVRGGNNVHQVRAEGMAVRHLFKVL
CCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KNGGTIGILPDQQPKIGEGVFAPFFGIEALTMTLIQRLAERTGATVLYAWCERISPHLEF
HCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHE
ALHMEHADPAVADPDPLIAATALNAGIERIARRDPTQYQWTYKRYSLRPPDSGEHNPYEI
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEE
KHQSHSIAKTLKHPD
CCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]