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The map label for this gene is glnS [H]

Identifier: 182681171

GI number: 182681171

Start: 725144

End: 726907

Strand: Reverse

Name: glnS [H]

Synonym: XfasM23_0613

Alternate gene names: 182681171

Gene position: 726907-725144 (Counterclockwise)

Preceding gene: 182681180

Following gene: 182681169

Centisome position: 28.67

GC content: 55.61

Gene sequence:

>1764_bases
ATGCACACCGTTCGACCCAGCATGTCCGAGATCACCACCACCGACGCCCAAGCCCAACCTGAGAAGAAAGACTTTATCCG
GCAGATCATCAGAGAAGACTTAGCCCACGGCACACACACTCACATCCACACCCGTTTTCCCCCAGAACCCAACGGTTACC
TGCACATTGGCCACGCCAAAGCAATCTGCCTGGATTTTGGCGTCGCCGCGGAATTCGGCGGACACTGCACCCTCCGCATG
GACGACACCAATCCGAGCAAAGAAGACCCCGCCTTCGCCGCTGCAATCCAGGAAGACGTCTCTTGGTTAGGCTTCCATTG
GAACGCACTACGACATACATCGGATTATTTCGAAGTACTGTACCTAGCTGCAGAAAAACTCATTGCCGATGGAAAAGCCT
ACGTCTGTGACCTCAATAGCCAACAGGTACGCGAATACCGCGGCACCCTGACAGAAGCGGGCCGTCCCTCACCATGGCGT
GAACGCAGCCCTGATGAAAATTTAGAACTATTCCGACAAATGCGCGCCGGCACGTTTCCAGACGGAACACGTACCCTGCG
TGCCAAAATCGACATGGCTAGCGGCAACATCAACCTGCGGGACCCAGCGCTCTACCGCATTAAACACGTCGAACATCAAA
ACACCGGCAACACCTGGCCGATCTACCCCATGTATGACTTCGCACACGCACTGAGCGATGCGATCGAAGGCATTACCCAT
TCATTGTGCACCCTTGAGTTCGAAGACCACCGCCCCCTGTACGACTGGTGTATCAACCATGTCGACCTACCAAACAACTC
ACACTTACTCAAACCGTTACTGGACAAAGGCTTCCCTCAAGAGCCCAGCCAACCTCGGCAAATCGAGTTTTCGCGCCTGA
ACATCAACTACACCGTGATGAGCAAACGCAAACTCACCGCACTGGTGGACGAAAAACTCGTCGAAGGGTGGGACGACCCC
CGCATGTACACCCTGCAAGGCCTACGCCGCCGTGGTTACACCCCAGCGGCGATGCGCCTATTCGTAGAGCGGATCGGCAT
CAGCAAACAGAACTCAATCATTGATTTTTCAGTACTAGAAAACTGCTTACGCGAGAACCTGGACACCATTGCACCACGCC
GGATGGCAACCATTGCACCAATGAAACTCGTGCTGACCAACCTAGCCGAAGACCATGAAGAACAACTGATCTTCCCCAAC
CATCCCAAAGACGACACACAAGGCACCCGAACAGTGCCGTTCTCACGCGAACTATGGATCGAACGCGATGATTTTTCCGA
AGCCCCCCCCAAGGGCTGGAAACGGCTGATTCCAGGCGGAGAAGTACGCCTACGCGGTGCCGGCATCGCACGTATAGATG
AGGTGGTGAAAAACGCAGAAGGCCACGTCATCGCACTACACGGCTGGCTTGATCCGACCTCACGTCCCGGCATGGAAGGC
GCTCACCGCAAAGTCAAAGGCACCATCCACTGGGTCAGCGCACCACACGCTGTAGCAGCGGAAATCCGCCTGTACGACCG
GCTGTTCTCCATTGAAAAACCAGACGACAACACCGACGGAAAAACCTACCGCGACTTCCTCAACCCAGACTCCAAACGCG
TCGTGCATGGCTACATCGAACCAGCGGCGGCACAAACCGCACCAGAACACGCATTCCAATTTGAACGCTTGGGCTACTTC
GTCACAGACCGGCATGACCACGATGCCACACACCCCGTCTTTAATCGCAGCGTCACGCTGCGCGACACCTGGCAGCGCGA
CTGA

Upstream 100 bases:

>100_bases
CACCATTCACACCCAAGATGCCGCACAAACAAAAATAAAATCCCTGCACACCTTGACGCATCCCGGGGCAAAACCTACGA
AGTTTAGCTAAACTCGCCAC

Downstream 100 bases:

>100_bases
ACCGCCGCGCCCAACATCAAAAACCAATATTAGTATTGCCAGTGCTCACCGTAGAGTGCCTGCAGCATGACATCCCATCG
ACCGCAAGAAAATTCTGCGT

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MHTVRPSMSEITTTDAQAQPEKKDFIRQIIREDLAHGTHTHIHTRFPPEPNGYLHIGHAKAICLDFGVAAEFGGHCTLRM
DDTNPSKEDPAFAAAIQEDVSWLGFHWNALRHTSDYFEVLYLAAEKLIADGKAYVCDLNSQQVREYRGTLTEAGRPSPWR
ERSPDENLELFRQMRAGTFPDGTRTLRAKIDMASGNINLRDPALYRIKHVEHQNTGNTWPIYPMYDFAHALSDAIEGITH
SLCTLEFEDHRPLYDWCINHVDLPNNSHLLKPLLDKGFPQEPSQPRQIEFSRLNINYTVMSKRKLTALVDEKLVEGWDDP
RMYTLQGLRRRGYTPAAMRLFVERIGISKQNSIIDFSVLENCLRENLDTIAPRRMATIAPMKLVLTNLAEDHEEQLIFPN
HPKDDTQGTRTVPFSRELWIERDDFSEAPPKGWKRLIPGGEVRLRGAGIARIDEVVKNAEGHVIALHGWLDPTSRPGMEG
AHRKVKGTIHWVSAPHAVAAEIRLYDRLFSIEKPDDNTDGKTYRDFLNPDSKRVVHGYIEPAAAQTAPEHAFQFERLGYF
VTDRHDHDATHPVFNRSVTLRDTWQRD

Sequences:

>Translated_587_residues
MHTVRPSMSEITTTDAQAQPEKKDFIRQIIREDLAHGTHTHIHTRFPPEPNGYLHIGHAKAICLDFGVAAEFGGHCTLRM
DDTNPSKEDPAFAAAIQEDVSWLGFHWNALRHTSDYFEVLYLAAEKLIADGKAYVCDLNSQQVREYRGTLTEAGRPSPWR
ERSPDENLELFRQMRAGTFPDGTRTLRAKIDMASGNINLRDPALYRIKHVEHQNTGNTWPIYPMYDFAHALSDAIEGITH
SLCTLEFEDHRPLYDWCINHVDLPNNSHLLKPLLDKGFPQEPSQPRQIEFSRLNINYTVMSKRKLTALVDEKLVEGWDDP
RMYTLQGLRRRGYTPAAMRLFVERIGISKQNSIIDFSVLENCLRENLDTIAPRRMATIAPMKLVLTNLAEDHEEQLIFPN
HPKDDTQGTRTVPFSRELWIERDDFSEAPPKGWKRLIPGGEVRLRGAGIARIDEVVKNAEGHVIALHGWLDPTSRPGMEG
AHRKVKGTIHWVSAPHAVAAEIRLYDRLFSIEKPDDNTDGKTYRDFLNPDSKRVVHGYIEPAAAQTAPEHAFQFERLGYF
VTDRHDHDATHPVFNRSVTLRDTWQRD
>Mature_587_residues
MHTVRPSMSEITTTDAQAQPEKKDFIRQIIREDLAHGTHTHIHTRFPPEPNGYLHIGHAKAICLDFGVAAEFGGHCTLRM
DDTNPSKEDPAFAAAIQEDVSWLGFHWNALRHTSDYFEVLYLAAEKLIADGKAYVCDLNSQQVREYRGTLTEAGRPSPWR
ERSPDENLELFRQMRAGTFPDGTRTLRAKIDMASGNINLRDPALYRIKHVEHQNTGNTWPIYPMYDFAHALSDAIEGITH
SLCTLEFEDHRPLYDWCINHVDLPNNSHLLKPLLDKGFPQEPSQPRQIEFSRLNINYTVMSKRKLTALVDEKLVEGWDDP
RMYTLQGLRRRGYTPAAMRLFVERIGISKQNSIIDFSVLENCLRENLDTIAPRRMATIAPMKLVLTNLAEDHEEQLIFPN
HPKDDTQGTRTVPFSRELWIERDDFSEAPPKGWKRLIPGGEVRLRGAGIARIDEVVKNAEGHVIALHGWLDPTSRPGMEG
AHRKVKGTIHWVSAPHAVAAEIRLYDRLFSIEKPDDNTDGKTYRDFLNPDSKRVVHGYIEPAAAQTAPEHAFQFERLGYF
VTDRHDHDATHPVFNRSVTLRDTWQRD

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=549, Percent_Identity=42.8051001821494, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI62241042, Length=559, Percent_Identity=33.4525939177102, Blast_Score=283, Evalue=3e-76,
Organism=Escherichia coli, GI1786895, Length=574, Percent_Identity=53.6585365853659, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1788743, Length=107, Percent_Identity=28.9719626168224, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17543332, Length=548, Percent_Identity=42.7007299270073, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17511007, Length=539, Percent_Identity=33.0241187384045, Blast_Score=252, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6324742, Length=607, Percent_Identity=36.5733113673806, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6321192, Length=545, Percent_Identity=32.8440366972477, Blast_Score=280, Evalue=5e-76,
Organism=Drosophila melanogaster, GI17864488, Length=558, Percent_Identity=39.7849462365591, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24649466, Length=535, Percent_Identity=32.1495327102804, Blast_Score=247, Evalue=2e-65,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 67171; Mature: 67171

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTVRPSMSEITTTDAQAQPEKKDFIRQIIREDLAHGTHTHIHTRFPPEPNGYLHIGHAK
CCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCE
AICLDFGVAAEFGGHCTLRMDDTNPSKEDPAFAAAIQEDVSWLGFHWNALRHTSDYFEVL
EEEEECCHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLAAEKLIADGKAYVCDLNSQQVREYRGTLTEAGRPSPWRERSPDENLELFRQMRAGTFP
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
DGTRTLRAKIDMASGNINLRDPALYRIKHVEHQNTGNTWPIYPMYDFAHALSDAIEGITH
CCCEEEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SLCTLEFEDHRPLYDWCINHVDLPNNSHLLKPLLDKGFPQEPSQPRQIEFSRLNINYTVM
HHEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEE
SKRKLTALVDEKLVEGWDDPRMYTLQGLRRRGYTPAAMRLFVERIGISKQNSIIDFSVLE
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEHHHHH
NCLRENLDTIAPRRMATIAPMKLVLTNLAEDHEEQLIFPNHPKDDTQGTRTVPFSRELWI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCEECCCCHHHEE
ERDDFSEAPPKGWKRLIPGGEVRLRGAGIARIDEVVKNAEGHVIALHGWLDPTSRPGMEG
ECCCCCCCCCHHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCC
AHRKVKGTIHWVSAPHAVAAEIRLYDRLFSIEKPDDNTDGKTYRDFLNPDSKRVVHGYIE
CCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEEEEECC
PAAAQTAPEHAFQFERLGYFVTDRHDHDATHPVFNRSVTLRDTWQRD
CHHHCCCHHHHHHHHHCCEEEECCCCCCCCCCHHCCEEEECCCCCCC
>Mature Secondary Structure
MHTVRPSMSEITTTDAQAQPEKKDFIRQIIREDLAHGTHTHIHTRFPPEPNGYLHIGHAK
CCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCE
AICLDFGVAAEFGGHCTLRMDDTNPSKEDPAFAAAIQEDVSWLGFHWNALRHTSDYFEVL
EEEEECCHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLAAEKLIADGKAYVCDLNSQQVREYRGTLTEAGRPSPWRERSPDENLELFRQMRAGTFP
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
DGTRTLRAKIDMASGNINLRDPALYRIKHVEHQNTGNTWPIYPMYDFAHALSDAIEGITH
CCCEEEEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SLCTLEFEDHRPLYDWCINHVDLPNNSHLLKPLLDKGFPQEPSQPRQIEFSRLNINYTVM
HHEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEE
SKRKLTALVDEKLVEGWDDPRMYTLQGLRRRGYTPAAMRLFVERIGISKQNSIIDFSVLE
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEHHHHH
NCLRENLDTIAPRRMATIAPMKLVLTNLAEDHEEQLIFPNHPKDDTQGTRTVPFSRELWI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCEECCCCHHHEE
ERDDFSEAPPKGWKRLIPGGEVRLRGAGIARIDEVVKNAEGHVIALHGWLDPTSRPGMEG
ECCCCCCCCCHHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCC
AHRKVKGTIHWVSAPHAVAAEIRLYDRLFSIEKPDDNTDGKTYRDFLNPDSKRVVHGYIE
CCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEEEEECC
PAAAQTAPEHAFQFERLGYFVTDRHDHDATHPVFNRSVTLRDTWQRD
CHHHCCCHHHHHHHHHCCEEEECCCCCCCCCCHHCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]