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The map label for this gene is nuoF [H]

Identifier: 182680810

GI number: 182680810

Start: 321120

End: 322454

Strand: Direct

Name: nuoF [H]

Synonym: XfasM23_0245

Alternate gene names: 182680810

Gene position: 321120-322454 (Clockwise)

Preceding gene: 182680809

Following gene: 182680811

Centisome position: 12.66

GC content: 53.78

Gene sequence:

>1335_bases
ATGGGACATCCTCACGTATCCAAGGGACCGGTTGGCCCGGCACCTCAGCCGCACCAAGTGATCTATACCACGTTGCATTA
TGAAATTCCTTGGTCGTATGAAAGCTATTTGAAGACCGGCGGCTACACTGCGTTACGCAAGATTTTTGAGGAAAAGATCC
CTCCGGAGCAGGTCATAGACATGGTCAAGCAGTCGAATCTGCGGGGTCGTGGTGGTGCAGGTTTTCCGACGGGTCTCAAA
TGGTCCTTCATGCCGAAGGGCACCATGCAAAAATATATTCTGTGTAACTCGGACGAGTCCGAACCGGGAACCTGTAAAGA
TCGTGACATCCTGCGCTATAACCCGCATTCCGTGGTGGAGGGGATGGCGATTGCATGTTATGCCACTGGTTCCACTGTGG
CTTACAACTATTTGCGTGGGGAGTTCCACCACGAGCCGTTCGAACATTTTGAACAGGCGCTGGCCGATGCTTACACACAT
GGTTGGCTTGGTAAGAGCGTTTTGGGAAGTGGCGTAGATATCGATATCTATGCGGTGCTTGGTGCTGGTGCCTACATCTG
TGGTGAAGAGACTGCGCTCATGGAGTCTCTGGAAGGCAAGAAGGGGCAGCCTCGCTACAAGCCCCCCTTTCCTGCCAATT
TTGGCTTGTACGGCAAGCCATCAACGATCAACAACACTGAGACGTATGCCGCAGTGCCAGCCATTATTCGCAATGGTCCG
GAGTGGTTTTTGTCTCTGAGCAAGACTAAAAACGGTGGCTCTAAGATCTTCTCCGTCTCGGGGTGCGTGCAGCAAGGGGG
TAACTTCGAGGTACCCTTAGGCACCACCTTTGATGAATTGCTGGAGATGGCTGGTGGCCTGCGGCCAGGTCGCGCTCTGA
AAGCTGCAATTCCGGGGGGAGTATCCATGCCGGTATTGAAAGCCGAGCAGTTGCAGGGCCTTCAGATGGACTACGACACA
CTGCGTGTGCTGGGTAGCGGTTTAGGCTCGGGGGGGATTGTTGTGCTTGATGACAGTGTCTGTTGTGTCCGTTTTGCCTG
CCGCATCTCGCAGTTTTTTCATAAGGAATCGTGTGGCCAGTGTACTCCGTGCCGTGAGGGGACCGGTTGGATGCACCGCG
TTTTGGAGCGTATCGTTGCTGGCCAGGCCACGATGGAGGATCTGCACCAGCTTAAAGTTGTGGCGGGGCAGATTGAGGGG
CATACCATTTGTGCGTTTGGTGAAGCTGCAGCTTGGCCAATCCAAGGGTTCCTGCGCCAATTCTGGGATGAATTTGAGTA
CTACATTGTTCATGGTCGGTCGATTGTGGATGACAAGGCGAGAGTGGCTGCATGA

Upstream 100 bases:

>100_bases
GCTTGTGCTGGTGCTCCGATGATGGTGATTAACGGTCATTATCATGAGCGTCTGACCAAGGAGAAGGTTGACCAGTTGTT
AGACGGCTTGGAGTAACGTC

Downstream 100 bases:

>100_bases
GTGACCAATCTGTGAATCCTGAGGTGCGGCCTGATCGTGTCACCATTGAAATTGATGGCCAGAATTTAGTTGTACCGAAA
GGTTCAATGATTATTCAGGC

Product: NADH-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MGHPHVSKGPVGPAPQPHQVIYTTLHYEIPWSYESYLKTGGYTALRKIFEEKIPPEQVIDMVKQSNLRGRGGAGFPTGLK
WSFMPKGTMQKYILCNSDESEPGTCKDRDILRYNPHSVVEGMAIACYATGSTVAYNYLRGEFHHEPFEHFEQALADAYTH
GWLGKSVLGSGVDIDIYAVLGAGAYICGEETALMESLEGKKGQPRYKPPFPANFGLYGKPSTINNTETYAAVPAIIRNGP
EWFLSLSKTKNGGSKIFSVSGCVQQGGNFEVPLGTTFDELLEMAGGLRPGRALKAAIPGGVSMPVLKAEQLQGLQMDYDT
LRVLGSGLGSGGIVVLDDSVCCVRFACRISQFFHKESCGQCTPCREGTGWMHRVLERIVAGQATMEDLHQLKVVAGQIEG
HTICAFGEAAAWPIQGFLRQFWDEFEYYIVHGRSIVDDKARVAA

Sequences:

>Translated_444_residues
MGHPHVSKGPVGPAPQPHQVIYTTLHYEIPWSYESYLKTGGYTALRKIFEEKIPPEQVIDMVKQSNLRGRGGAGFPTGLK
WSFMPKGTMQKYILCNSDESEPGTCKDRDILRYNPHSVVEGMAIACYATGSTVAYNYLRGEFHHEPFEHFEQALADAYTH
GWLGKSVLGSGVDIDIYAVLGAGAYICGEETALMESLEGKKGQPRYKPPFPANFGLYGKPSTINNTETYAAVPAIIRNGP
EWFLSLSKTKNGGSKIFSVSGCVQQGGNFEVPLGTTFDELLEMAGGLRPGRALKAAIPGGVSMPVLKAEQLQGLQMDYDT
LRVLGSGLGSGGIVVLDDSVCCVRFACRISQFFHKESCGQCTPCREGTGWMHRVLERIVAGQATMEDLHQLKVVAGQIEG
HTICAFGEAAAWPIQGFLRQFWDEFEYYIVHGRSIVDDKARVAA
>Mature_443_residues
GHPHVSKGPVGPAPQPHQVIYTTLHYEIPWSYESYLKTGGYTALRKIFEEKIPPEQVIDMVKQSNLRGRGGAGFPTGLKW
SFMPKGTMQKYILCNSDESEPGTCKDRDILRYNPHSVVEGMAIACYATGSTVAYNYLRGEFHHEPFEHFEQALADAYTHG
WLGKSVLGSGVDIDIYAVLGAGAYICGEETALMESLEGKKGQPRYKPPFPANFGLYGKPSTINNTETYAAVPAIIRNGPE
WFLSLSKTKNGGSKIFSVSGCVQQGGNFEVPLGTTFDELLEMAGGLRPGRALKAAIPGGVSMPVLKAEQLQGLQMDYDTL
RVLGSGLGSGGIVVLDDSVCCVRFACRISQFFHKESCGQCTPCREGTGWMHRVLERIVAGQATMEDLHQLKVVAGQIEGH
TICAFGEAAAWPIQGFLRQFWDEFEYYIVHGRSIVDDKARVAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=410, Percent_Identity=45.8536585365854, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI260656005, Length=410, Percent_Identity=45.8536585365854, Blast_Score=377, Evalue=1e-104,
Organism=Escherichia coli, GI1788620, Length=418, Percent_Identity=41.866028708134, Blast_Score=321, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI17531727, Length=410, Percent_Identity=43.9024390243902, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19920794, Length=422, Percent_Identity=44.5497630331754, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45550452, Length=440, Percent_Identity=43.6363636363636, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24653819, Length=417, Percent_Identity=35.4916067146283, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24653817, Length=358, Percent_Identity=35.7541899441341, Blast_Score=231, Evalue=9e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 48589; Mature: 48458

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHPHVSKGPVGPAPQPHQVIYTTLHYEIPWSYESYLKTGGYTALRKIFEEKIPPEQVID
CCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHH
MVKQSNLRGRGGAGFPTGLKWSFMPKGTMQKYILCNSDESEPGTCKDRDILRYNPHSVVE
HHHHCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEECCHHHHC
GMAIACYATGSTVAYNYLRGEFHHEPFEHFEQALADAYTHGWLGKSVLGSGVDIDIYAVL
CCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEEE
GAGAYICGEETALMESLEGKKGQPRYKPPFPANFGLYGKPSTINNTETYAAVPAIIRNGP
CCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCH
EWFLSLSKTKNGGSKIFSVSGCVQQGGNFEVPLGTTFDELLEMAGGLRPGRALKAAIPGG
HHEEEEECCCCCCCEEEEEHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCCCEEEECCCC
VSMPVLKAEQLQGLQMDYDTLRVLGSGLGSGGIVVLDDSVCCVRFACRISQFFHKESCGQ
CCCCEECHHHHCCCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCC
CTPCREGTGWMHRVLERIVAGQATMEDLHQLKVVAGQIEGHTICAFGEAAAWPIQGFLRQ
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
FWDEFEYYIVHGRSIVDDKARVAA
HHHCCEEEEEECCEEECCHHHHCC
>Mature Secondary Structure 
GHPHVSKGPVGPAPQPHQVIYTTLHYEIPWSYESYLKTGGYTALRKIFEEKIPPEQVID
CCCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHH
MVKQSNLRGRGGAGFPTGLKWSFMPKGTMQKYILCNSDESEPGTCKDRDILRYNPHSVVE
HHHHCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEECCHHHHC
GMAIACYATGSTVAYNYLRGEFHHEPFEHFEQALADAYTHGWLGKSVLGSGVDIDIYAVL
CCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEEE
GAGAYICGEETALMESLEGKKGQPRYKPPFPANFGLYGKPSTINNTETYAAVPAIIRNGP
CCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCH
EWFLSLSKTKNGGSKIFSVSGCVQQGGNFEVPLGTTFDELLEMAGGLRPGRALKAAIPGG
HHEEEEECCCCCCCEEEEEHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCCCEEEECCCC
VSMPVLKAEQLQGLQMDYDTLRVLGSGLGSGGIVVLDDSVCCVRFACRISQFFHKESCGQ
CCCCEECHHHHCCCCCCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCC
CTPCREGTGWMHRVLERIVAGQATMEDLHQLKVVAGQIEGHTICAFGEAAAWPIQGFLRQ
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
FWDEFEYYIVHGRSIVDDKARVAA
HHHCCEEEEEECCEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA